BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,215	A→G	R522R (CGT→CGC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,215	0	A	G	100.0%	864.3 / NA	268	R522R (CGT→CGC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (125/143); total (125/143)

Only 100 of 281 total aligned reads displayed.

CCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTT  >  NC_000913/64125‑64304

  aGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCa                                                                                       <  2:2190661/93‑1 (MQ=255)

   gACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCAt                                                                                      >  2:5971401/1‑93 (MQ=255)

     cAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATgg                                                                                    >  1:2494869/1‑93 (MQ=255)

     cAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATgg                                                                                    >  1:4418401/1‑93 (MQ=255)

      aaaCGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGt                                                                                   <  1:873502/93‑1 (MQ=255)

      aaaCGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGt                                                                                   >  2:3108020/1‑93 (MQ=255)

        aCGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGa                                                                                 <  1:2715061/93‑1 (MQ=255)

         cGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGAt                                                                                >  2:4759820/1‑93 (MQ=255)

         cGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGAt                                                                                <  1:1652864/93‑1 (MQ=255)

           ttGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcg                                                                              >  1:5190820/1‑93 (MQ=255)

           ttGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcg                                                                              >  1:5328228/1‑93 (MQ=255)

            tGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcga                                                                             <  1:62025/93‑1 (MQ=255)

              aGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcgacg                                                                           <  1:4939163/93‑1 (MQ=255)

              aGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcgacg                                                                           <  2:2125598/93‑1 (MQ=255)

               gTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcgacga                                                                          >  2:1149923/1‑93 (MQ=255)

               gTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATcgacga                                                                          <  1:3331998/93‑1 (MQ=255)

                tCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGAAGAt                                                                         >  1:3178821/1‑93 (MQ=255)

                   gTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGcc                                                                      <  1:6299856/93‑1 (MQ=255)

                    tagcGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCa                                                                     <  1:190010/90‑1 (MQ=255)

                    tATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCa                                                                     <  2:5122268/93‑1 (MQ=255)

                    tATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCa                                                                     >  1:5798515/1‑93 (MQ=255)

                     aTCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAg                                                                    <  2:2003893/93‑1 (MQ=255)

                      tCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGCGATCGACGATGCCAGc                                                                   >  1:5738637/1‑93 (MQ=255)

                       cGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGcc                                                                  <  2:4012192/93‑1 (MQ=255)

                           gTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCgg                                                              >  1:2504661/1‑93 (MQ=255)

                           gTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCgg                                                              >  1:539305/1‑93 (MQ=255)

                            tAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGa                                                             >  2:1667854/1‑93 (MQ=255)

                            tAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGa                                                             <  1:2232392/93‑1 (MQ=255)

                             aGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGAt                                                            <  2:3775920/93‑1 (MQ=255)

                              gATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATc                                                           >  2:2430574/1‑93 (MQ=255)

                                tAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCga                                                         <  2:5274235/93‑1 (MQ=255)

                                  aCGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCgaag                                                       >  1:4858166/1‑93 (MQ=255)

                                   cgtcgtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCgaaga                                                      >  1:503235/1‑93 (MQ=255)

                                   cgtcgtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCgaaga                                                      <  1:3047705/93‑1 (MQ=255)

                                    gtcgtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCgaagaa                                                     >  1:1857335/1‑93 (MQ=255)

                                    gtcgtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCgaagaa                                                     <  1:4149809/93‑1 (MQ=255)

                                    gtcgtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCgaagaa                                                     >  2:5493311/1‑93 (MQ=255)

                                      cgtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGc                                                   >  1:231380/1‑93 (MQ=255)

                                       gtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCg                                                  >  1:1433056/1‑93 (MQ=255)

                                       gtAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCg                                                  <  1:5625756/93‑1 (MQ=255)

                                         aGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGc                                                >  2:543580/1‑93 (MQ=255)

                                          gCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCa                                               <  2:2768524/93‑1 (MQ=255)

                                          gCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCa                                               >  1:1326988/1‑93 (MQ=255)

                                           cccTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAg                                              <  2:154232/93‑1 (MQ=255)

                                            ccTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAgg                                             >  1:78822/1‑93 (MQ=255)

                                            ccTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAgg                                             >  1:5481630/1‑93 (MQ=255)

                                             cTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGTCGCGGATCGAAGAAGCGGCAGGc                                            >  2:480110/1‑93 (MQ=255)

                                             cTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGc                                            >  2:5700505/1‑93 (MQ=255)

                                             cTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGc                                            >  1:4968700/1‑93 (MQ=255)

                                             cTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGc                                            <  1:3731686/93‑1 (MQ=255)

                                              tgtgCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCg                                           >  1:5927914/1‑93 (MQ=255)

                                               gtgCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCgg                                          >  2:4555908/1‑93 (MQ=255)

                                               gtgCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCgg                                          <  2:2933018/93‑1 (MQ=255)

                                               gtgCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCgg                                          <  1:457929/93‑1 (MQ=255)

                                               gtgCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCgg                                          >  1:663900/1‑93 (MQ=255)

                                                 tcTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtg                                        <  2:2533421/92‑1 (MQ=255)

                                                 gCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtg                                        <  1:2184324/93‑1 (MQ=255)

                                                 gCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtg                                        <  1:3859814/93‑1 (MQ=255)

                                                  cTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtgg                                       <  2:1506069/93‑1 (MQ=255)

                                                  cTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtgg                                       <  2:4197260/93‑1 (MQ=255)

                                                   ttCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGGATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtggg                                      >  1:3389233/1‑92 (MQ=255)

                                                   ttCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCggtggt                                      <  2:3678745/93‑1 (MQ=255)

                                                    tcaatcaaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGgtggtg                                     >  1:1025703/1‑93 (MQ=255)

                                                    tcaatcaaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGgtggtg                                     >  2:2500911/1‑93 (MQ=255)

                                                      aatcaaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGcc                                   >  2:1709963/1‑93 (MQ=255)

                                                      aatcaaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGcc                                   >  1:1166862/1‑93 (MQ=255)

                                                       atcaaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCg                                  >  2:318468/1‑93 (MQ=255)

                                                        tcatCGCTTTGTTTTGCCGCATGATCGGATGGCCGCGCATGGTGATCGACGATGCCACCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGa                                 <  2:4252087/93‑1 (MQ=255)

                                                        tcaaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGa                                 <  1:22801/93‑1 (MQ=255)

                                                        tcaaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGa                                 <  2:4931009/93‑1 (MQ=255)

                                                          aaCGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGc                               <  2:1326179/93‑1 (MQ=255)

                                                            cGCTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCAc                             <  1:4322436/93‑1 (MQ=255)

                                                              cTTTGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATCCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGc                           <  2:2225850/93‑1 (MQ=255)

                                                               tttGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGcc                          <  2:3810786/93‑1 (MQ=255)

                                                                ttGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGGCGAGCACGCCa                         >  2:1261460/1‑93 (MQ=255)

                                                                ttGGTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCa                         <  2:3413456/93‑1 (MQ=255)

                                                                  ggTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATa                       <  2:5559399/93‑1 (MQ=255)

                                                                  ggTTTGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATa                       >  2:1207288/1‑93 (MQ=255)

                                                                    tttGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATaaa                     <  1:344954/93‑1 (MQ=255)

                                                                     ttGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATaaaa                    <  1:594442/93‑1 (MQ=255)

                                                                     ttGCCGCATAATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATaaaa                    <  2:6196683/93‑1 (MQ=255)

                                                                      tGCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAg                   >  2:2797196/1‑93 (MQ=255)

                                                                       gCCGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAgg                  <  1:3626668/93‑1 (MQ=255)

                                                                         cGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCa                <  2:3000295/93‑1 (MQ=255)

                                                                         cGCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCa                <  2:4475996/93‑1 (MQ=255)

                                                                          gCATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCAt               >  1:4679926/1‑93 (MQ=255)

                                                                           cATGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCAtt              >  1:4785233/1‑93 (MQ=255)

                                                                            aTGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGCGCACCCCATAAAAGGGATTc             >  2:847919/1‑93 (MQ=255)

                                                                            aTGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTc             <  2:1008688/93‑1 (MQ=255)

                                                                            aTGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTc             <  1:5115284/93‑1 (MQ=255)

                                                                             tGATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCCTTCa            >  2:598880/1‑93 (MQ=255)

                                                                              gATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCat           <  1:3926713/93‑1 (MQ=255)

                                                                              gATCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCat           >  2:5785296/1‑93 (MQ=255)

                                                                               aTCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCatg          >  2:25967/1‑93 (MQ=255)

                                                                               aTCTGATGACCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGGGCCGAGCACGCCATAAAAGGCATTCatg          >  1:1071381/1‑93 (MQ=255)

                                                                                  tgatgaCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCatgatg       <  1:2982632/93‑1 (MQ=255)

                                                                                    atgaCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATgatgat     >  1:2031188/1‑93 (MQ=255)

                                                                                       aCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGAtttt  >  1:3809823/1‑93 (MQ=255)

                                                                                       aCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGAtttt  >  1:2080009/1‑93 (MQ=255)

                                                                                       aCCGCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGAtttt  <  1:15680/93‑1 (MQ=255)

CCAGACAAACGTTGAGTCGGTATCGCCGTAGATAACGTCGTAGCCCTGTGCTTCAATCAACGCTTTGGTTTGCCGCATGATCTGATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTT  >  NC_000913/64125‑64304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`