BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	500,613	A→G	E422G (GAG→GGG)	gsk →	inosine‑guanosine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	500,613	0	A	G	99.6%	710.2 / NA	222	E422G (GAG→GGG)	gsk	inosine‑guanosine kinase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (119/102); total (120/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

CATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCA  >  NC_000913/500523‑500702

  tCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGt                                                                                       >  1:5329996/1‑93 (MQ=255)

   cGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTg                                                                                      >  2:4876697/1‑93 (MQ=255)

   cGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTg                                                                                      <  1:2405605/93‑1 (MQ=255)

     ttAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGaa                                                                                    >  1:397030/1‑93 (MQ=255)

     ttAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGaa                                                                                    <  1:5462173/93‑1 (MQ=255)

     ttAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGaa                                                                                    >  1:321946/1‑93 (MQ=255)

      tAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAg                                                                                   >  1:6042148/1‑93 (MQ=255)

       aGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGa                                                                                  <  2:2472092/93‑1 (MQ=255)

       aGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGa                                                                                  <  1:3008184/93‑1 (MQ=255)

        gcgcAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGAc                                                                                 <  2:348952/93‑1 (MQ=255)

         ggcAGGTGTGTAAATATGCGAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGTCGTGAAGACa                                                                                <  2:5537231/92‑1 (MQ=255)

         cgcAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACa                                                                                >  1:1704840/1‑93 (MQ=255)

          gcAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAg                                                                               >  2:2949433/1‑93 (MQ=255)

          gcAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAg                                                                               >  1:254858/1‑93 (MQ=255)

           cAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGc                                                                              >  2:355575/1‑93 (MQ=255)

            aGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTGCTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGcc                                                                             <  1:1130050/93‑1 (MQ=255)

            aGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGcc                                                                             <  2:1166076/93‑1 (MQ=255)

             ggTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCt                                                                            >  2:5168290/1‑93 (MQ=255)

             ggTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCt                                                                            >  1:1548456/1‑93 (MQ=255)

              gtgtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTg                                                                           <  1:575622/93‑1 (MQ=255)

              gtgtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTg                                                                           <  2:4238915/93‑1 (MQ=255)

              gtgtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTg                                                                           >  1:2353188/1‑93 (MQ=255)

                gtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGa                                                                         <  1:539629/93‑1 (MQ=255)

                gtgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGa                                                                         >  2:2194637/1‑93 (MQ=255)

                 tgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGaa                                                                        <  1:4225884/93‑1 (MQ=255)

                 tgtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGaa                                                                        <  2:3162462/93‑1 (MQ=255)

                  gtAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAag                                                                       <  2:4152111/93‑1 (MQ=255)

                   tAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAaga                                                                      <  1:3459851/93‑1 (MQ=255)

                    aaaTATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAagag                                                                     <  1:4945448/93‑1 (MQ=255)

                     aaTATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGt                                                                    <  2:4349174/93‑1 (MQ=255)

                     aaTATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGt                                                                    >  1:96532/1‑93 (MQ=255)

                     aaTATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGt                                                                    <  2:3937864/93‑1 (MQ=255)

                      atatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTc                                                                   <  2:1927716/93‑1 (MQ=255)

                       tatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCt                                                                  >  2:2871280/1‑93 (MQ=255)

                       tatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCt                                                                  >  2:5384183/1‑93 (MQ=255)

                       tatGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCt                                                                  <  1:2640523/93‑1 (MQ=255)

                        atGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCtt                                                                 <  2:6275271/93‑1 (MQ=255)

                         tGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTa                                                                >  2:4524475/1‑93 (MQ=255)

                          gCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTAc                                                               >  1:4828118/1‑93 (MQ=255)

                           cTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACt                                                              >  2:1502266/1‑93 (MQ=255)

                             aaCCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTgg                                                            >  1:4779654/1‑93 (MQ=255)

                              aCCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTggg                                                           >  1:1814178/1‑93 (MQ=255)

                                 gtgtGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATc                                                        <  1:887789/93‑1 (MQ=255)

                                 gtgtGAGCTATCAGGGACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGGGAAAACAGCCTGGAAGAGACTTACTGGGGTc                                                        >  2:3655859/1‑93 (MQ=255)

                                  tgtgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCg                                                       >  1:2868115/1‑93 (MQ=255)

                                    tgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGtt                                                     >  2:5804309/1‑93 (MQ=255)

                                    tgAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGtt                                                     >  1:1480878/1‑93 (MQ=255)

                                     gAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTa                                                    >  2:1809857/1‑93 (MQ=255)

                                     gAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGGGTTACTGGGGTAGTTa                                                    >  2:2309364/1‑93 (MQ=255)

                                        cTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGt                                                 >  1:670237/1‑93 (MQ=255)

                                        cTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGt                                                 <  1:3813060/93‑1 (MQ=255)

                                         tATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGtt                                                >  1:2232148/1‑93 (MQ=255)

                                           tCAGGTACTGAACCAGCATTCCCCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAt                                              <  1:1124992/93‑1 (MQ=255)

                                            cAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtc                                             <  1:5432058/93‑1 (MQ=255)

                                            cAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtc                                             <  1:2805918/93‑1 (MQ=255)

                                             aGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcg                                            <  1:4682685/93‑1 (MQ=255)

                                              ggTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgt                                           >  1:2530792/1‑93 (MQ=255)

                                              ggTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgt                                           <  2:1751780/93‑1 (MQ=255)

                                              ggTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgt                                           >  2:702124/1‑93 (MQ=255)

                                              ggTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgt                                           >  1:4066547/1‑93 (MQ=255)

                                               gTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgtc                                          <  1:5951320/93‑1 (MQ=255)

                                               gTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTAtcgtc                                          <  1:2991598/93‑1 (MQ=255)

                                                 aCTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCgg                                        >  2:5827261/1‑93 (MQ=255)

                                                 aCTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCgg                                        <  2:4543487/93‑1 (MQ=255)

                                                 aCTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCgg                                        <  1:4372259/93‑1 (MQ=255)

                                                  cTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGt                                       <  2:4803882/93‑1 (MQ=255)

                                                  cTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGt                                       <  1:3186557/93‑1 (MQ=255)

                                                     aaCCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCg                                    >  1:4600046/1‑93 (MQ=255)

                                                     aaCCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCg                                    >  2:3987154/1‑93 (MQ=255)

                                                      aCCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGt                                   >  1:3486637/1‑93 (MQ=255)

                                                       ccAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTa                                  <  2:3579682/93‑1 (MQ=255)

                                                       ccAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTa                                  >  2:5675857/1‑93 (MQ=255)

                                                          gCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGc                               <  1:2055975/93‑1 (MQ=255)

                                                          gCATTCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGc                               >  2:1306761/1‑93 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            >  2:5924623/1‑93 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            >  1:176001/1‑93 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            >  2:1560609/1‑93 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAg                            >  2:3794561/1‑93 (MQ=255)

                                                             ttCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGGTAAGTTATCGTCGGTTCGTAGGCCAg                            >  1:4120232/1‑93 (MQ=255)

                                                              tCACCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGa                           >  1:1976177/1‑93 (MQ=255)

                                                                cccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATa                         <  2:3619742/92‑1 (MQ=255)

                                                                aCCTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATa                         >  1:6217705/1‑93 (MQ=255)

                                                                 ccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATaa                        >  2:3672387/1‑93 (MQ=255)

                                                                 ccTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATaa                        >  1:921846/1‑93 (MQ=255)

                                                                  cTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAg                       <  1:4494158/93‑1 (MQ=255)

                                                                  cTCGTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAg                       >  1:2012303/1‑93 (MQ=255)

                                                                     gTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCg                    >  2:782550/1‑93 (MQ=255)

                                                                     gTTTAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCg                    >  1:1371805/1‑93 (MQ=255)

                                                                      tttAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGt                   >  2:368017/1‑93 (MQ=255)

                                                                      tttAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGt                   >  2:4074356/1‑93 (MQ=255)

                                                                      tttAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGt                   >  2:238880/1‑93 (MQ=255)

                                                                       ttAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGtt                  <  1:733247/93‑1 (MQ=255)

                                                                       ttAACGCGCGGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGtt                  >  2:2049293/1‑93 (MQ=255)

                                                                            gcgcgGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCAcgc             <  1:3694570/93‑1 (MQ=255)

                                                                            gcgcgGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCAcgc             <  1:4985573/93‑1 (MQ=255)

                                                                             cgcgGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCAcgcc            >  1:5188565/1‑93 (MQ=255)

                                                                              gcgGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCAcgccg           >  1:3617898/1‑93 (MQ=255)

                                                                               cgGCTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCAcgccgc          >  2:3317602/1‑93 (MQ=255)

                                                                                  cTTGCCGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATTGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATc       <  2:724961/93‑1 (MQ=255)

                                                                                       cGGGGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCa  <  1:3793788/93‑1 (MQ=255)

CATCGTTAGCGCAGGTGTGTAAATATGCTAACCGTGTGAGCTATCAGGTACTGAACCAGCATTCACCTCGTTTAACGCGCGGCTTGCCGGAGCGTGAAGACAGCCTGGAAGAGTCTTACTGGGATCGTTAAGTTATCGTCGGTTCGTAGGCCAGATAAGGCGTTCACGCCGCATCTGGCA  >  NC_000913/500523‑500702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`