BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,134,509	A→G	E274E (GAA→GAG)	b3943 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,134,509	0	A	G	99.4%	566.5 / ‑4.9	176	E274E (GAA→GAG)	b3943	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (69/106); total (70/106)
Fisher's exact test for biased strand distribution p-value = 3.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 190 total aligned reads displayed.

CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTT  >  NC_000913/4134419‑4134598

cAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAggg                                                                                         <  1:5542629/93‑1 (MQ=255)

 aGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGt                                                                                        <  2:2530198/93‑1 (MQ=255)

  gTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTa                                                                                       <  1:5305727/93‑1 (MQ=255)

   tATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAg                                                                                      >  1:5127297/1‑93 (MQ=255)

   tATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAg                                                                                      <  2:3533057/93‑1 (MQ=255)

    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGc                                                                                     >  2:4200983/1‑93 (MQ=255)

    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGc                                                                                     <  1:4037329/93‑1 (MQ=255)

    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGc                                                                                     >  2:289559/1‑93 (MQ=255)

    aTTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGc                                                                                     >  2:5941790/1‑93 (MQ=255)

      tGCCAGCTTAAGCACACTGTCCGTTCCCGTTTGCGGCGTCCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCgg                                                                                   <  1:803193/93‑1 (MQ=255)

      tGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCgg                                                                                   <  2:2265966/93‑1 (MQ=255)

        ccAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCggga                                                                                 >  2:2689023/1‑91 (MQ=255)

         cAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTat                                                                                >  1:2044719/1‑93 (MQ=255)

          aGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTCCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTatt                                                                               >  2:4312603/1‑93 (MQ=255)

           gCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattg                                                                              >  2:1172863/1‑93 (MQ=255)

            cTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgt                                                                             <  2:2091089/93‑1 (MQ=255)

              tAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgc                                                                           <  1:3378538/93‑1 (MQ=255)

              tAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgc                                                                           >  2:903956/1‑93 (MQ=255)

               aaGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgct                                                                          >  1:379427/1‑93 (MQ=255)

                 gCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctga                                                                        <  2:1610319/93‑1 (MQ=255)

                  cacaCTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgat                                                                       <  1:3904359/93‑1 (MQ=255)

                       tGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgc                                                                  >  1:5761205/1‑93 (MQ=255)

                        ggCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTattgtgctgattgtgct                                                                 >  1:2586456/1‑93 (MQ=255)

                          ccGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTgg                                                               <  2:4229298/93‑1 (MQ=255)

                           cGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGc                                                              >  1:4404000/1‑93 (MQ=255)

                           cGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGc                                                              >  2:3377746/1‑93 (MQ=255)

                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            >  2:3178827/1‑93 (MQ=255)

                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            >  1:2824915/1‑93 (MQ=255)

                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            >  2:4758740/1‑93 (MQ=255)

                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            >  2:73387/1‑93 (MQ=255)

                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            >  2:7846/1‑93 (MQ=255)

                             ttCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCAc                                                            <  1:1272218/93‑1 (MQ=255)

                               cccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTg                                                          <  2:1398131/93‑1 (MQ=255)

                               cccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTg                                                          >  1:5468159/1‑93 (MQ=255)

                                ccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTgg                                                         <  2:28384/93‑1 (MQ=255)

                                ccGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACCgg                                                         <  1:2555717/93‑1 (MQ=255)

                                  gTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGgcg                                                       >  1:1680129/1‑93 (MQ=255)

                                    ttGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCt                                                     <  1:2409776/93‑1 (MQ=255)

                                     tGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTg                                                    <  2:2310516/93‑1 (MQ=255)

                                      gcggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTgg                                                   <  1:4362966/93‑1 (MQ=255)

                                      gcggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTgg                                                   <  1:4552123/93‑1 (MQ=255)

                                       gggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGt                                                  <  1:3311965/92‑1 (MQ=255)

                                        ggcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTg                                                 >  1:6054405/1‑93 (MQ=255)

                                        cgcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTg                                                 <  1:5937848/92‑1 (MQ=255)

                                         gcgTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGa                                                <  2:4686040/93‑1 (MQ=255)

                                           gTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGc                                              <  2:852352/93‑1 (MQ=255)

                                            tACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGcc                                             >  2:3169889/1‑93 (MQ=255)

                                            tACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGcc                                             <  2:2368144/93‑1 (MQ=255)

                                             aCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCg                                            >  1:457093/1‑93 (MQ=255)

                                             aCTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCg                                            <  2:4353136/93‑1 (MQ=255)

                                              ctctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGt                                           >  1:2119658/1‑93 (MQ=255)

                                              ctctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGt                                           <  2:5333538/93‑1 (MQ=255)

                                               tctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTa                                          >  2:3843688/1‑93 (MQ=255)

                                                ctTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTaa                                         <  2:2075229/93‑1 (MQ=255)

                                                 ttttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAg                                        <  1:1577147/93‑1 (MQ=255)

                                                 ttttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAg                                        >  1:4868124/1‑93 (MQ=255)

                                                  tttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGa                                       <  1:2888876/93‑1 (MQ=255)

                                                  tttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGa                                       <  2:5003297/93‑1 (MQ=255)

                                                   ttCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaa                                      <  1:929501/93‑1 (MQ=255)

                                                    tCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaa                                     <  1:1189493/93‑1 (MQ=255)

                                                    tCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaa                                     >  1:6174626/1‑93 (MQ=255)

                                                     ccTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaa                                    <  2:2118005/93‑1 (MQ=255)

                                                      cTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaa                                   <  2:1109588/93‑1 (MQ=255)

                                                      cTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaa                                   <  1:5371166/93‑1 (MQ=255)

                                                       tggtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGaaaaaa                                  >  1:1110659/1‑93 (MQ=255)

                                                        ggtggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaag                                 <  1:4112168/93‑1 (MQ=255)

                                                          tggCTGCTCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAaagaa                               <  1:2263337/93‑1 (MQ=255)

                                                            gctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAACAAGAAGc                             >  1:179543/1‑93 (MQ=255)

                                                            gctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGc                             <  1:1913296/93‑1 (MQ=255)

                                                            gctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGc                             <  1:1721170/93‑1 (MQ=255)

                                                             ctgctCGGCGAGAGTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGcc                            <  1:127684/93‑1 (MQ=255)

                                                             ctgctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGcc                            <  2:6109192/93‑1 (MQ=255)

                                                             ctgctAGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGcc                            <  2:5763472/93‑1 (MQ=255)

                                                              tgctCGGCGAGAATCCGGGGGCCGTTGAGGTTAGCGGTATTGTGCTGATTGTGCTGGCACGGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCg                           <  1:3841969/93‑1 (MQ=255)

                                                               gctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGt                          <  2:3241004/93‑1 (MQ=255)

                                                               gctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGt                          <  1:2619075/93‑1 (MQ=255)

                                                                ctCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTc                         <  2:4171581/93‑1 (MQ=255)

                                                                ctCAGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTc                         <  1:6300140/93‑1 (MQ=255)

                                                                 tgggCGAGAATCCGGGGGCTGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCa                        <  2:1788810/91‑1 (MQ=255)

                                                                 tCGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCa                        <  2:1874848/93‑1 (MQ=255)

                                                                 gcGGCGAGAATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCa                        <  1:4982768/92‑1 (MQ=255)

                                                                        gaATCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaa                 <  1:852062/93‑1 (MQ=255)

                                                                          aTCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTaaaaa               >  1:5229891/1‑93 (MQ=255)

                                                                           tCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAg              <  1:3043353/93‑1 (MQ=255)

                                                                           tCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAg              >  1:2629813/1‑93 (MQ=255)

                                                                           tCCGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAg              <  2:286397/93‑1 (MQ=255)

                                                                            ccGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAgg             <  2:4858479/93‑1 (MQ=255)

                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            <  2:4159076/93‑1 (MQ=255)

                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            >  1:5603255/1‑93 (MQ=255)

                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            <  1:5842155/93‑1 (MQ=255)

                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            <  1:2056877/93‑1 (MQ=255)

                                                                             cGGGGGCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGa            <  2:28188/93‑1 (MQ=255)

                                                                               ggggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATc          >  2:4096378/1‑93 (MQ=255)

                                                                                gggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCt         <  2:926867/93‑1 (MQ=255)

                                                                                 ggCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTg        <  2:1825808/93‑1 (MQ=255)

                                                                                  gCCGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGa       >  1:6042067/1‑93 (MQ=255)

                                                                                    cGTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAt     <  2:2449549/93‑1 (MQ=255)

                                                                                     gTTGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAtt    <  1:2439103/93‑1 (MQ=255)

                                                                                      ttGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAttt   >  1:1964121/1‑93 (MQ=255)

                                                                                      ttGAGGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAAttt   >  2:454337/1‑93 (MQ=255)

CAGTATTGCCAGCTTAAGCACACTGGCCGTTCCCGTTTGCGGCGTACTCTTTTCCTGGTGGCTGCTCGGCGAGAATCCGGGGGCCGTTGAAGGTAGCGGTATTGTGCTGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTT  >  NC_000913/4134419‑4134598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`