BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,171,379	C→T	A240V (GCC→GTC)	birA →	biotin‑[acetyl‑CoA carboxylase] holoenzyme synthetase and biotin operon repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,171,379	0	C	T	100.0%	853.3 / NA	234	A240V (GCC→GTC)	birA	biotin‑[acetyl‑CoA carboxylase] holoenzyme synthetase and biotin operon repressor
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (108/126); total (108/126)

Only 100 of 246 total aligned reads displayed.

CAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATA  >  NC_000913/4171289‑4171468

cAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCa                                                                                         >  1:2037397/1‑93 (MQ=255)

 aaTGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCAt                                                                                        >  2:2482708/1‑93 (MQ=255)

 aaTGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCAt                                                                                        >  1:1984352/1‑93 (MQ=255)

 aaTGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCAt                                                                                        >  2:5326399/1‑93 (MQ=255)

  aTGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATg                                                                                       >  2:2022206/1‑93 (MQ=255)

  aTGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATg                                                                                       >  2:3114509/1‑93 (MQ=255)

  aTGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATg                                                                                       >  1:2934297/1‑93 (MQ=255)

   tGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGc                                                                                      >  1:3592584/1‑93 (MQ=255)

    gcgcCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCt                                                                                     <  1:3837634/93‑1 (MQ=255)

     cgcCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATTAATCTCGATCGTAATACGTTGGCGGTCATGCTa                                                                                    <  1:6144149/93‑1 (MQ=255)

     cgcCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTa                                                                                    <  1:5886498/93‑1 (MQ=255)

      gcCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTaa                                                                                   <  2:4833541/93‑1 (MQ=255)

      gcCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTaa                                                                                   <  2:5174040/93‑1 (MQ=255)

      gcCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTaa                                                                                   <  2:5379362/93‑1 (MQ=255)

       ccGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAAt                                                                                  <  2:464963/93‑1 (MQ=255)

       ccGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAAt                                                                                  >  2:882651/1‑93 (MQ=255)

        cGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATa                                                                                 >  2:5858160/1‑93 (MQ=255)

         gtgtTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATAc                                                                                >  1:362574/1‑93 (MQ=255)

          tgtTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACg                                                                               >  2:5620666/1‑93 (MQ=255)

          tgtTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACg                                                                               >  2:3867237/1‑93 (MQ=255)

          tgtTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACg                                                                               <  2:5789215/93‑1 (MQ=255)

            ttGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTg                                                                             >  2:4511172/1‑93 (MQ=255)

              gAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGaa                                                                           <  2:3360983/93‑1 (MQ=255)

                agagagTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAAtt                                                                         >  2:3452834/1‑93 (MQ=255)

                   gagTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACg                                                                      <  2:3027556/93‑1 (MQ=255)

                      tgtCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGtgc                                                                   <  2:2237712/93‑1 (MQ=255)

                       gtCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGtgct                                                                  <  2:5372392/93‑1 (MQ=255)

                        tCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGtgctg                                                                 >  1:5452718/1‑93 (MQ=255)

                         cGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGtgctgc                                                                <  1:2903037/93‑1 (MQ=255)

                         cGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGtgctgc                                                                <  1:4038656/93‑1 (MQ=255)

                         cGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGtgctgc                                                                <  2:2057632/93‑1 (MQ=255)

                          gTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCg                                                               >  2:2651776/1‑93 (MQ=255)

                              aTCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTgg                                                           <  1:4239503/93‑1 (MQ=255)

                              aTCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGAGAATTACGTGCTGCGTTgg                                                           <  2:6145580/93‑1 (MQ=255)

                                cAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGaa                                                         <  2:60973/93‑1 (MQ=255)

                                cAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGaa                                                         >  1:552226/1‑93 (MQ=255)

                                 aGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAAc                                                        >  2:1349549/1‑93 (MQ=255)

                                 aGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAAc                                                        >  2:3600247/1‑93 (MQ=255)

                                 aGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAAc                                                        <  1:5705852/93‑1 (MQ=255)

                                 aGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAAc                                                        >  1:4921436/1‑93 (MQ=255)

                                 aGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAAc                                                        <  2:5055197/93‑1 (MQ=255)

                                  ggggTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAAct                                                       >  1:172346/1‑93 (MQ=255)

                                  ggggTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAAct                                                       >  1:648812/1‑93 (MQ=255)

                                     gTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCtt                                                    >  1:4380163/1‑93 (MQ=255)

                                     gTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATCACGTGCTGCGTTGGAACTCtt                                                    <  2:4010472/93‑1 (MQ=255)

                                       ggATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCg                                                  >  2:1777439/1‑93 (MQ=255)

                                       ggATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCg                                                  >  2:1239731/1‑93 (MQ=255)

                                        gATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGa                                                 <  2:3192141/93‑1 (MQ=255)

                                        gATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGa                                                 >  1:452304/1‑93 (MQ=255)

                                         aTCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGaa                                                <  1:4682173/93‑1 (MQ=255)

                                         aTCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGaa                                                <  1:442618/93‑1 (MQ=255)

                                          tCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAAc                                               <  2:5578307/93‑1 (MQ=255)

                                           cACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACa                                              >  2:2915425/1‑93 (MQ=255)

                                           cACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACa                                              <  1:3332997/93‑1 (MQ=255)

                                              gCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGCACTCTTCGAACaaga                                           >  2:3317010/1‑93 (MQ=255)

                                              gCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACaaga                                           >  2:3808547/1‑93 (MQ=255)

                                                tGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACaagaag                                         <  2:2142691/93‑1 (MQ=255)

                                                 gCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAgg                                        <  1:5498359/93‑1 (MQ=255)

                                                 gCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAgg                                        >  1:3694266/1‑93 (MQ=255)

                                                  cAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGa                                       <  1:5794232/93‑1 (MQ=255)

                                                   aGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGAt                                      <  2:2783960/93‑1 (MQ=255)

                                                   aGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGCTGGAACTCTTCGAACAAGAAGGAt                                      <  1:1302314/93‑1 (MQ=255)

                                                    ggAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGAtt                                     >  2:1682805/1‑93 (MQ=255)

                                                    ggAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGAtt                                     <  2:4342057/93‑1 (MQ=255)

                                                     gAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTg                                    <  2:2400563/93‑1 (MQ=255)

                                                     gAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTg                                    <  2:2855379/93‑1 (MQ=255)

                                                     gAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTg                                    >  1:3515319/1‑93 (MQ=255)

                                                     gAAGCGGGGATAAATCTCGATCGTAATAGGTTGGCGGTCATGCTAATTCGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGCATTg                                    >  2:4427195/1‑93 (MQ=255)

                                                          ggcgATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCAcc                               <  1:1922355/90‑1 (MQ=255)

                                                           gggATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCt                              <  1:1376182/93‑1 (MQ=255)

                                                           gggATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCt                              <  1:5267916/93‑1 (MQ=255)

                                                             gATCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTa                            <  1:2733051/93‑1 (MQ=255)

                                                              aTCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTAt                           <  2:6051621/93‑1 (MQ=255)

                                                               tCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATc                          <  2:4789310/93‑1 (MQ=255)

                                                               tCAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATc                          <  1:710764/93‑1 (MQ=255)

                                                                cAATCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCt                         <  1:5651718/93‑1 (MQ=255)

                                                                 aaTCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTg                        >  2:1902983/1‑93 (MQ=255)

                                                                 aaTCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTg                        <  1:4613839/93‑1 (MQ=255)

                                                                  aTCTCGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGt                       >  1:776045/1‑93 (MQ=255)

                                                                      cGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTcgcg                   >  1:6222668/1‑93 (MQ=255)

                                                                      cGATCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTcgcg                   >  2:1613472/1‑93 (MQ=255)

                                                                       gATCGTAATACGTGGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCgcgc                  <  1:5404337/93‑1 (MQ=255)

                                                                        aTCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCt                 <  1:1632182/93‑1 (MQ=255)

                                                                        aTCGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCt                 <  2:2371486/93‑1 (MQ=255)

                                                                          cGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTgg               <  2:3663781/93‑1 (MQ=255)

                                                                          cGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTgg               >  2:3387966/1‑93 (MQ=255)

                                                                          cGTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTgg               <  1:866105/93‑1 (MQ=255)

                                                                           gTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTggg              >  1:487829/1‑93 (MQ=255)

                                                                           gTAATACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTggg              >  1:2354308/1‑93 (MQ=255)

                                                                             aaTACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGaa            >  1:4969812/1‑93 (MQ=255)

                                                                              aTACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGaaa           <  1:558190/93‑1 (MQ=255)

                                                                               tACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGaaaa          <  1:4074674/93‑1 (MQ=255)

                                                                               tACGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGaaaa          >  1:1009723/1‑93 (MQ=255)

                                                                                 cGTTGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGc        <  2:694043/93‑1 (MQ=255)

                                                                                   ttGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTg      <  1:4264653/93‑1 (MQ=255)

                                                                                    tGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTgg     >  1:1862884/1‑93 (MQ=255)

                                                                                    tGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTgg     >  2:3004521/1‑93 (MQ=255)

                                                                                    tGGCGGTCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTgg     <  2:2335572/93‑1 (MQ=255)

                                                                                     ggcggtCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGa    <  2:5936910/93‑1 (MQ=255)

                                                                                      gcggtCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGAt   <  1:2486917/93‑1 (MQ=255)

CAATGCGCCGTGTTGAAGAGAGTGTCGTTAATCAGGGGTGGATCACGCTGCAGGAAGCGGGGATCAATCTCGATCGTAATACGTTGGCGGCCATGCTAATACGTGAATTACGTGCTGCGTTGGAACTCTTCGAACAAGAAGGATTGGCACCTTATCTGTCGCGCTGGGAAAAGCTGGATA  >  NC_000913/4171289‑4171468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`