BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,222,747	T→C	C447C (TGT→TGC)	metH →	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,222,747	0	T	C	100.0%	769.0 / NA	234	C447C (TGT→TGC)	metH	B12‑dependent homocysteine‑N5‑methyltetrahydrofolate transmethylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (119/115); total (119/115)

Only 100 of 245 total aligned reads displayed.

CAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGG  >  NC_000913/4222657‑4222836

cAATCTGATTGCCGGTGAACCGGATATCTCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCAt                                                                                         <  2:6300175/93‑1 (MQ=255)

  aTCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATcc                                                                                       <  2:743307/93‑1 (MQ=255)

   tCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCa                                                                                      <  1:4137965/93‑1 (MQ=255)

   tCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCa                                                                                      <  1:634826/93‑1 (MQ=255)

    cTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAg                                                                                     <  2:808678/93‑1 (MQ=255)

    cTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAg                                                                                     <  1:4979506/93‑1 (MQ=255)

     tGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCAAgg                                                                                    >  1:5271958/1‑93 (MQ=255)

      gATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAggg                                                                                   >  2:2853389/1‑93 (MQ=255)

      gATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAggg                                                                                   >  2:5520197/1‑93 (MQ=255)

       aTTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGc                                                                                  >  1:5173530/1‑93 (MQ=255)

       aTTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGc                                                                                  >  1:465658/1‑93 (MQ=255)

          gCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCaaa                                                                               <  1:5953265/93‑1 (MQ=255)

             ggTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGc                                                                            <  2:2460643/93‑1 (MQ=255)

               tGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCAt                                                                          <  2:2050984/93‑1 (MQ=255)

               tGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCAt                                                                          <  1:4449867/93‑1 (MQ=255)

               tGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCAt                                                                          <  1:3078114/93‑1 (MQ=255)

                  aCCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGt                                                                       <  2:4913103/93‑1 (MQ=255)

                  aCCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGt                                                                       >  1:5777973/1‑93 (MQ=255)

                  aCCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGt                                                                       >  1:480499/1‑93 (MQ=255)

                    cGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTa                                                                     <  1:4836073/93‑1 (MQ=255)

                    cGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTa                                                                     >  1:1602631/1‑93 (MQ=255)

                    cGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTa                                                                     >  2:3104244/1‑93 (MQ=255)

                     ggATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTaa                                                                    <  1:4737712/93‑1 (MQ=255)

                     ggATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTaa                                                                    <  2:4860541/93‑1 (MQ=255)

                      gATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAAc                                                                   <  2:1998235/93‑1 (MQ=255)

                      gATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAAc                                                                   >  2:5882746/1‑93 (MQ=255)

                        tatCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAActc                                                                 <  2:3978313/93‑1 (MQ=255)

                         atCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAActct                                                                >  2:5656446/1‑93 (MQ=255)

                         atCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAActct                                                                >  1:3357501/1‑93 (MQ=255)

                         atCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAActct                                                                <  1:1870079/93‑1 (MQ=255)

                          tcgctcgcGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTa                                                               >  2:4572511/1‑93 (MQ=255)

                               cgcgTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCg                                                          >  1:5354264/1‑93 (MQ=255)

                                gcgTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGa                                                         <  1:1506299/93‑1 (MQ=255)

                                gcgTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGa                                                         >  1:4885365/1‑93 (MQ=255)

                                gcgTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGa                                                         <  1:2499047/93‑1 (MQ=255)

                                 cgTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGTGCAAAGGCATTGTTAACTCTATCTCGAt                                                        >  1:2773362/1‑93 (MQ=255)

                                 cgTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGAt                                                        <  1:430179/93‑1 (MQ=255)

                                 cgTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGAt                                                        <  2:5576476/93‑1 (MQ=255)

                                   tGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGa                                                      <  1:2128603/93‑1 (MQ=255)

                                   tGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGa                                                      >  1:2862087/1‑93 (MQ=255)

                                   tGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGa                                                      <  1:1171924/93‑1 (MQ=255)

                                    gCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGaa                                                     >  2:5427568/1‑93 (MQ=255)

                                    gCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAACGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGaa                                                     <  2:6213910/93‑1 (MQ=255)

                                     ccGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGaaa                                                    <  2:400293/93‑1 (MQ=255)

                                     ccGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGaaa                                                    <  2:5054371/93‑1 (MQ=255)

                                       gATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAaga                                                  >  1:2647155/1‑93 (MQ=255)

                                        aTTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAagag                                                 <  2:3169639/93‑1 (MQ=255)

                                        aTTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAagag                                                 >  2:5379061/1‑93 (MQ=255)

                                        aTTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAagag                                                 <  2:3376913/93‑1 (MQ=255)

                                        aTTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAagag                                                 <  2:383817/93‑1 (MQ=255)

                                         ttATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGTGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAgg                                                >  1:3990633/1‑93 (MQ=255)

                                         ttATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAgg                                                >  1:743555/1‑93 (MQ=255)

                                         ttATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAgg                                                >  1:1153033/1‑93 (MQ=255)

                                         ttATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAgg                                                <  1:2585775/93‑1 (MQ=255)

                                         ttATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAgg                                                >  1:4271367/1‑93 (MQ=255)

                                          tATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAggg                                               >  1:2299208/1‑93 (MQ=255)

                                          tATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAggg                                               <  1:3491884/93‑1 (MQ=255)

                                          tATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAggg                                               <  2:4699722/93‑1 (MQ=255)

                                          tATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAggg                                               <  2:3753108/93‑1 (MQ=255)

                                           aTGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGc                                              >  2:5722228/1‑93 (MQ=255)

                                           aTGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGc                                              >  2:2231728/1‑93 (MQ=255)

                                              aTCGACTCCTCAAAATGGGACGTCATTGAAAAAGTTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTc                                           <  2:2398171/93‑1 (MQ=255)

                                              aTCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTc                                           <  1:598519/93‑1 (MQ=255)

                                               tCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCg                                          >  2:6096110/1‑93 (MQ=255)

                                                cGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGa                                         <  2:4131467/93‑1 (MQ=255)

                                                cGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGa                                         <  2:4519363/93‑1 (MQ=255)

                                                 gACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGAt                                        >  1:5891563/1‑93 (MQ=255)

                                                 gACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGAt                                        >  1:3597780/1‑93 (MQ=255)

                                                   ctcctcAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGc                                      >  2:4397242/1‑93 (MQ=255)

                                                     cctcAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCt                                    >  1:2157141/1‑93 (MQ=255)

                                                     cctcAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCt                                    >  1:6107520/1‑93 (MQ=255)

                                                     cctcAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCt                                    <  2:4255559/93‑1 (MQ=255)

                                                      ctcAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCtt                                   >  1:4380662/1‑93 (MQ=255)

                                                        cAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTa                                 <  1:1678751/93‑1 (MQ=255)

                                                        cAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTa                                 <  2:2356286/93‑1 (MQ=255)

                                                          aaaTGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATc                               <  2:1510207/93‑1 (MQ=255)

                                                           aaTGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATcc                              >  2:3407277/1‑93 (MQ=255)

                                                            aTGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCa                             <  1:6227100/93‑1 (MQ=255)

                                                             tGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCAt                            >  1:3964639/1‑93 (MQ=255)

                                                             tGGGACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCAt                            >  2:5988462/1‑93 (MQ=255)

                                                                gACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCAc                         >  2:1301380/1‑93 (MQ=255)

                                                                gACGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCAc                         >  1:3231975/1‑93 (MQ=255)

                                                                  cGTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCAcgc                       >  2:1757848/1‑93 (MQ=255)

                                                                   gTCATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCAcgcg                      >  2:3904063/1‑93 (MQ=255)

                                                                     cATTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGaa                    <  1:1362662/93‑1 (MQ=255)

                                                                      aTTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGaaa                   >  2:3674038/1‑93 (MQ=255)

                                                                      aTTGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGaaa                   >  2:5260804/1‑93 (MQ=255)

                                                                        tGAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAAtt                 <  1:2079511/93‑1 (MQ=255)

                                                                         gAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAAttg                >  1:3406059/1‑93 (MQ=255)

                                                                         gAAAAAGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAAttg                >  1:3765878/1‑93 (MQ=255)

                                                                            aaaGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAAttgttg             >  2:2291808/1‑93 (MQ=255)

                                                                            aaaGGTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAAttgttg             >  2:3568297/1‑93 (MQ=255)

                                                                             aaGCTCTGAAGTGCATCCAGCGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGc            <  2:2582511/93‑1 (MQ=255)

                                                                               ggTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGt          <  1:2542914/93‑1 (MQ=255)

                                                                               ggTCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGt          <  1:2893252/93‑1 (MQ=255)

                                                                                 tCTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCg        >  2:4430979/1‑93 (MQ=255)

                                                                                  cTGAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCGATCACGCGAAATTGTTGCGTCGc       <  1:2167765/93‑1 (MQ=255)

                                                                                    gAAGTGCATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTa     >  2:1400709/1‑93 (MQ=255)

                                                                                      aGTGCATCCAGGGCAAAGCCATTGTTAACTCTATCTCGATGAAAGAGGGCTTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACg   <  2:1040232/93‑1 (MQ=255)

                                                                                       gtgcATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACgg  >  1:1000834/1‑93 (MQ=255)

CAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGG  >  NC_000913/4222657‑4222836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`