BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,244,543	T→C	F61L (TTC→CTC)	malK →	ATP‑binding component of transport system for maltose

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,244,543	0	T	C	100.0%	744.6 / NA	229	F61L (TTC→CTC)	malK	ATP‑binding component of transport system for maltose
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (101/128); total (101/128)

Only 100 of 231 total aligned reads displayed.

TTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTG  >  NC_000913/4244453‑4244632

ttCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTc                                                                                         >  2:1326296/1‑93 (MQ=255)

 tCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCa                                                                                        <  2:5964617/93‑1 (MQ=255)

  cGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCAt                                                                                       <  1:4030280/93‑1 (MQ=255)

   gtggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATc                                                                                      <  1:1598443/93‑1 (MQ=255)

    tggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCg                                                                                     >  2:6154152/1‑93 (MQ=255)

    tggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCg                                                                                     <  2:1208201/93‑1 (MQ=255)

    tggtgTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCg                                                                                     <  2:4201764/93‑1 (MQ=255)

        gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 >  2:4679699/1‑93 (MQ=255)

        gtTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTga                                                                                 <  2:4265764/93‑1 (MQ=255)

         tttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTgag                                                                                >  1:3733277/1‑93 (MQ=255)

         tttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTgag                                                                                <  2:4103895/93‑1 (MQ=255)

          ttGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTgaga                                                                               <  2:4438643/93‑1 (MQ=255)

           tGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGaa                                                                              <  1:2524235/93‑1 (MQ=255)

             tCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAAc                                                                            >  2:165777/1‑93 (MQ=255)

               ggcccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGAGTGCCGGGCTTGAGAAGATCACCAGCGGCGCCCTGCTCATCCATGAGAAACgg                                                                          >  2:2137512/4‑93 (MQ=37)

               ggACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACgg                                                                          >  1:1183272/1‑93 (MQ=255)

               ggACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACgg                                                                          >  1:3984815/1‑93 (MQ=255)

                 aCCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGat                                                                        <  1:2471815/93‑1 (MQ=255)

                  ccGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatg                                                                       <  1:1330178/93‑1 (MQ=255)

                   cGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatga                                                                      <  1:3203323/93‑1 (MQ=255)

                     tCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaat                                                                    >  1:3912185/1‑93 (MQ=255)

                      cTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatg                                                                   <  2:134458/93‑1 (MQ=255)

                       tGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatgc                                                                  >  1:2587410/1‑92 (MQ=255)

                       tGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGatgaatga                                                                  >  2:2815218/1‑93 (MQ=255)

                        ggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGac                                                                 >  1:2150499/1‑93 (MQ=255)

                        ggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGac                                                                 >  2:5808567/1‑93 (MQ=255)

                        ggCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGac                                                                 <  2:4773921/93‑1 (MQ=255)

                         gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                                <  1:625592/93‑1 (MQ=255)

                         gCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGaca                                                                >  1:4525011/1‑93 (MQ=255)

                           tGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACt                                                              >  2:1859948/1‑93 (MQ=255)

                            gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTc                                                             <  2:1664501/93‑1 (MQ=255)

                            gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTc                                                             <  2:3815548/93‑1 (MQ=255)

                            gCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTc                                                             <  1:6069908/93‑1 (MQ=255)

                              ggTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCg                                                           >  1:3669982/1‑93 (MQ=255)

                               gTAACTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGc                                                          <  1:2970544/93‑1 (MQ=255)

                               gTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGc                                                          <  2:891703/93‑1 (MQ=255)

                                tAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGcc                                                         <  2:2446825/93‑1 (MQ=255)

                                   aTCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagc                                                      >  1:1000062/1‑93 (MQ=255)

                                    tCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagca                                                     <  1:1275523/93‑1 (MQ=255)

                                    tCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCcagca                                                     <  2:3046942/93‑1 (MQ=255)

                                       aCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaa                                                  <  2:2659772/93‑1 (MQ=255)

                                       aCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaa                                                  >  2:1549489/1‑93 (MQ=255)

                                       aCTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGaa                                                  <  1:981492/93‑1 (MQ=255)

                                        cTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAc                                                 <  1:1893433/93‑1 (MQ=255)

                                        cTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAc                                                 >  2:3519787/1‑93 (MQ=255)

                                         tttACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcg                                                >  1:2862989/1‑93 (MQ=255)

                                         tttACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcg                                                >  2:1295353/1‑93 (MQ=255)

                                          ttACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcgc                                               <  1:4388097/93‑1 (MQ=255)

                                          ttACTGCGCATGATTGCCGGGCTTGAGACGAGCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAAcgc                                               <  2:3189696/93‑1 (MQ=255)

                                           tACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGAAACTCGGACAGCAGAAcgcg                                              >  2:3097659/1‑93 (MQ=37)

                                            aCTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcgg                                             <  2:2904932/93‑1 (MQ=255)

                                            aCTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcgg                                             >  1:3775354/1‑93 (MQ=255)

                                             cTGCGCATGATTGCCGGGCTTGAGACGATCGCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggc                                            <  2:5913338/93‑1 (MQ=255)

                                             cTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggc                                            <  2:3033778/93‑1 (MQ=255)

                                              tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggcg                                           <  1:1132276/93‑1 (MQ=255)

                                              tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggcg                                           <  1:4712163/93‑1 (MQ=255)

                                              tGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACgcggcg                                           >  1:2734477/1‑93 (MQ=255)

                                                    tGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTa                                     >  2:2397948/1‑93 (MQ=255)

                                                    tGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTa                                     <  2:2763121/93‑1 (MQ=255)

                                                     gATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTAt                                    >  1:3246957/1‑93 (MQ=255)

                                                       ttGCCGGGCTTGATACGATCACCATCGGCGACCTACTCATCGGTGAGAAACGGATGAATGACACACCGCCAGCAGAACGCGGCGTTGGTATgg                                  <  2:4148328/93‑1 (MQ=255)

                                                       ttGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATgg                                  <  2:1642475/93‑1 (MQ=255)

                                                        tGCCGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgt                                 <  2:3606200/93‑1 (MQ=255)

                                                          ccGGGCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGgtgt                               >  1:5049916/1‑93 (MQ=255)

                                                            gggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGTGGCGTTGGTATGGTGttt                             >  1:1971368/1‑93 (MQ=255)

                                                             ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTc                            <  2:5306330/93‑1 (MQ=255)

                                                             ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTc                            >  2:2801442/1‑93 (MQ=255)

                                                             ggCTTGAGACGATCACCAGCGGCGACCTGCTCATCCGTGAGAAACGGATGAATGACAATCCGCCAGCAGAACGCTGCGATGGTATGGTGTTTc                            >  2:2263851/1‑93 (MQ=255)

                                                              gCTTGAGACTATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCa                           <  2:5405693/93‑1 (MQ=255)

                                                              gCTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCa                           >  1:591362/1‑93 (MQ=255)

                                                               cTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAg                          >  1:2062463/1‑93 (MQ=255)

                                                               cTTGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAg                          <  1:2236572/93‑1 (MQ=255)

                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         <  2:755490/93‑1 (MQ=255)

                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         <  2:5485346/93‑1 (MQ=255)

                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         >  1:5577932/1‑93 (MQ=255)

                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         >  2:366876/1‑93 (MQ=255)

                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         >  2:5129513/1‑93 (MQ=255)

                                                                ttGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGt                         >  1:1627736/1‑93 (MQ=255)

                                                                 tGAGACGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTc                        <  2:1599905/93‑1 (MQ=255)

                                                                    gaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTa                     <  1:1039718/93‑1 (MQ=255)

                                                                    gaCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTa                     <  1:5389176/93‑1 (MQ=255)

                                                                     aCGATCGCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACGCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAc                    <  1:2208839/93‑1 (MQ=255)

                                                                     aCGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAc                    <  1:5149149/93‑1 (MQ=255)

                                                                      cGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcg                   <  1:4424934/93‑1 (MQ=255)

                                                                      cGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcg                   <  2:4559878/93‑1 (MQ=255)

                                                                      cGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcg                   <  1:3902821/93‑1 (MQ=255)

                                                                      cGATCACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTAcc                   >  1:447977/1‑92 (MQ=255)

                                                                          cACCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGct               <  2:3649010/93‑1 (MQ=255)

                                                                           cccAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGctc              <  1:1693978/92‑1 (MQ=255)

                                                                           aCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGctc              <  1:1286806/93‑1 (MQ=255)

                                                                           aCCAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGctc              <  1:498994/93‑1 (MQ=255)

                                                                             cAGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTa            >  1:3323054/1‑93 (MQ=255)

                                                                              aGCGGCGACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTAt           <  2:397422/93‑1 (MQ=255)

                                                                               gcggcgACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATc          <  2:520080/93‑1 (MQ=255)

                                                                                    gACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAc     >  1:2027624/1‑93 (MQ=255)

                                                                                    gACCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAc     <  2:3517580/93‑1 (MQ=255)

                                                                                     aCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAcc    <  2:4484131/93‑1 (MQ=255)

                                                                                     aCCTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCAcc    <  1:5334662/93‑1 (MQ=255)

                                                                                      ccTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCt   <  1:4602430/93‑1 (MQ=255)

                                                                                       cTGCTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTg  >  1:3482097/1‑93 (MQ=255)

TTCGTGGTGTTTGTCGGACCGTCTGGCTGCGGTAAATCGACTTTACTGCGCATGATTGCCGGGCTTGAGACGATCACCAGCGGCGACCTGTTCATCGGTGAGAAACGGATGAATGACACTCCGCCAGCAGAACGCGGCGTTGGTATGGTGTTTCAGTCTTACGCGCTCTATCCCCACCTG  >  NC_000913/4244453‑4244632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`