BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,284,190	T→C	N254S (AAC→AGC)	acs ←	acetyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,284,190	0	T	C	100.0%	634.2 / NA	196	N254S (AAC→AGC)	acs	acetyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (97/99); total (97/99)

Only 100 of 200 total aligned reads displayed.

TACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCG  >  NC_000913/4284100‑4284279

tACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTc                                                                                         >  2:2505792/1‑93 (MQ=255)

 acacCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCa                                                                                        <  2:4836977/93‑1 (MQ=255)

   acCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCAtc                                                                                      <  1:254493/93‑1 (MQ=255)

    ccAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCAtct                                                                                     >  2:1490561/1‑93 (MQ=255)

     cAGATATCCCCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCAtctc                                                                                    <  2:3340910/93‑1 (MQ=255)

      aGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATctct                                                                                   <  2:3972447/93‑1 (MQ=255)

      aGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATctct                                                                                   >  1:3404782/1‑93 (MQ=255)

       gATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCtt                                                                                  <  2:408455/93‑1 (MQ=255)

       gATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCtt                                                                                  >  1:950029/1‑93 (MQ=255)

       gATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGCAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCtt                                                                                  >  1:4439009/1‑93 (MQ=255)

        aTAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTc                                                                                 <  1:1141281/93‑1 (MQ=255)

        aTAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTc                                                                                 <  2:3376906/93‑1 (MQ=255)

        aTAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTc                                                                                 <  1:4977540/93‑1 (MQ=255)

        aTAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTc                                                                                 <  1:3074269/93‑1 (MQ=255)

        aTAACCGCCGGTAGTACGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTc                                                                                 <  1:2621443/93‑1 (MQ=255)

         tAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTcc                                                                                >  2:5992420/1‑93 (MQ=255)

            ccgccgGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGcc                                                                             <  2:1908898/93‑1 (MQ=255)

            ccgccgGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGcc                                                                             <  1:2016018/93‑1 (MQ=255)

            ccgccgGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTGCGGCGCTCATCTCTTCCGcc                                                                             <  2:1822167/93‑1 (MQ=255)

             cgccgGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCt                                                                            >  1:5820677/1‑93 (MQ=255)

              gcgggTAGTATGC‑GCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTgg                                                                          <  1:3081391/90‑1 (MQ=255)

                cgGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTTATCTCTTCCGCCTggg                                                                         >  2:455728/1‑92 (MQ=255)

                 ggTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTg                                                                        >  1:1388846/1‑93 (MQ=255)

                 ggTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTg                                                                        >  1:1961849/1‑93 (MQ=255)

                 ggTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTg                                                                        <  1:6066952/93‑1 (MQ=255)

                  gtagtaTGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGc                                                                       <  2:670658/93‑1 (MQ=255)

                   tagtaTGCAGCACACCTTTTGGCTTACCGGTAGATCCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCt                                                                      <  1:1825006/93‑1 (MQ=255)

                   tagtaTGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCt                                                                      <  1:3421205/93‑1 (MQ=255)

                   tagtaTGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCt                                                                      >  1:4388174/1‑93 (MQ=255)

                    agtaTGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTg                                                                     <  2:4135000/93‑1 (MQ=255)

                    agtaTGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTg                                                                     >  2:5003187/1‑93 (MQ=255)

                     gtaTGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGa                                                                    <  1:2963925/93‑1 (MQ=255)

                     gtaTGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGGGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGa                                                                    >  2:3416369/1‑93 (MQ=255)

                      taTGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGAt                                                                   <  1:1680705/93‑1 (MQ=255)

                       aTGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCGTCGGCGCTCATCGCTTCCGCCTGGTGCTGAtc                                                                  <  1:4685601/93‑1 (MQ=255)

                         gcagcaCACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGAtcgc                                                                <  2:5649539/93‑1 (MQ=255)

                         gcagcaCACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGAtcgc                                                                >  1:2114716/1‑93 (MQ=255)

                          cagcaCACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGAtcgct                                                               <  2:5765979/93‑1 (MQ=255)

                           agcaCACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGAtcgctc                                                              <  1:1281954/93‑1 (MQ=255)

                            gcaCACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGAtcgctcg                                                             >  2:4255589/1‑93 (MQ=255)

                            gcaCACCTTTTGGCTTACCGGTAGAACCGGAGGGGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGAtcgctcg                                                             >  1:357902/1‑93 (MQ=255)

                             cacaCCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGAtcgctcgc                                                            >  2:773404/1‑93 (MQ=255)

                              acacCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATcgctcgct                                                           >  1:4091408/1‑93 (MQ=255)

                               cacCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCtt                                                          <  1:4047315/93‑1 (MQ=255)

                                acCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTg                                                         <  2:3796690/93‑1 (MQ=255)

                                acCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTg                                                         >  1:2793002/1‑93 (MQ=255)

                                 ccTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGc                                                        <  1:5545353/93‑1 (MQ=255)

                                  cTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTGGCTTGCt                                                       >  2:1427147/1‑93 (MQ=255)

                                   ttttGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTc                                                      >  2:5286337/1‑93 (MQ=255)

                                     ttGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCaa                                                    <  1:3237055/93‑1 (MQ=255)

                                        gCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCa                                                 <  2:5427826/93‑1 (MQ=255)

                                        gCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCa                                                 <  2:2265433/93‑1 (MQ=255)

                                         cTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAg                                                <  2:4186910/93‑1 (MQ=255)

                                          ttACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAgg                                               >  2:2303578/1‑93 (MQ=255)

                                           tACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGt                                              >  1:102015/1‑93 (MQ=255)

                                             ccGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCg                                            >  1:3319662/1‑93 (MQ=255)

                                              cGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGt                                           >  2:4019757/1‑93 (MQ=255)

                                               ggTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTg                                          <  2:465824/93‑1 (MQ=255)

                                                gTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGAGCGCTCGCTTGCTCAACCAGGTCGTGc                                         >  2:5092663/1‑93 (MQ=255)

                                                 tAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGcc                                        >  2:6207500/1‑93 (MQ=255)

                                                  aGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGcca                                       >  1:5334653/1‑93 (MQ=255)

                                                     aCCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGccacca                                    >  2:5546851/1‑93 (MQ=255)

                                                      ccGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCcaccac                                   <  2:1271622/93‑1 (MQ=255)

                                                       cGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACcaca                                  >  2:295859/1‑93 (MQ=255)

                                                       cGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCACTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACcaca                                  <  1:3259790/93‑1 (MQ=255)

                                                        ggAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAg                                 >  2:536262/1‑93 (MQ=255)

                                                         gAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAgg                                <  1:678720/93‑1 (MQ=255)

                                                         gAGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAgg                                <  1:639601/93‑1 (MQ=255)

                                                          aGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGt                               <  2:934360/93‑1 (MQ=255)

                                                          aGGTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGt                               <  2:4238381/93‑1 (MQ=255)

                                                           ggTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTc                              >  1:4909578/1‑93 (MQ=255)

                                                           ggTGTAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTc                              >  1:5884094/1‑93 (MQ=255)

                                                            gtgtAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTcg                             >  2:4347757/1‑93 (MQ=255)

                                                             tgtAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTcgc                            >  1:5051902/1‑93 (MQ=255)

                                                             tgtAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTcgc                            <  2:5785665/93‑1 (MQ=255)

                                                              gtAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTcgcg                           >  2:2573494/1‑93 (MQ=255)

                                                              gtAGAGAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGGcgcg                           >  2:378022/1‑93 (MQ=255)

                                                                agagAATAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGcc                         >  1:2481050/1‑93 (MQ=255)

                                                                    aaTAAACAGCGGATGTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTc                     >  2:1696025/1‑93 (MQ=255)

                                                                     aTAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTcc                    <  1:2805808/93‑1 (MQ=255)

                                                                     aTAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTcc                    <  1:3473183/93‑1 (MQ=255)

                                                                     aTAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTcc                    <  1:2185123/93‑1 (MQ=255)

                                                                      tAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCt                   <  1:2057911/93‑1 (MQ=255)

                                                                      tAAACAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGAGCCCTTCCt                   >  2:2995810/1‑93 (MQ=255)

                                                                       aaaCAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTg                  <  1:5368136/93‑1 (MQ=255)

                                                                          cAGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCa               <  1:3519659/93‑1 (MQ=255)

                                                                           aGCGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAg              >  2:1705456/1‑93 (MQ=255)

                                                                             cGGATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTc            <  1:4226746/93‑1 (MQ=255)

                                                                               gATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCaa          >  1:2293694/1‑93 (MQ=255)

                                                                               gATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCaa          <  1:2355543/93‑1 (MQ=255)

                                                                               gATCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCaa          <  1:6175526/93‑1 (MQ=255)

                                                                                aTCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAAt         <  2:4465937/93‑1 (MQ=255)

                                                                                 tCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAAtt        >  1:5295898/1‑93 (MQ=255)

                                                                                 tCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAAtt        >  2:5144322/1‑93 (MQ=255)

                                                                                 tCTTCGGCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAAtt        >  2:4614497/1‑93 (MQ=255)

                                                                                      ggCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTccc   <  1:5212133/93‑1 (MQ=255)

                                                                                      ggCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTccc   >  2:1978255/1‑93 (MQ=255)

                                                                                      ggCGCTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTccc   <  2:2380266/93‑1 (MQ=255)

                                                                                       gcgcTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCg  >  1:3701388/1‑93 (MQ=255)

                                                                                       gcgcTCATCTCTTCCGACTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGGCGCGCCCTTACTGCCAGTCAATTTTCCCg  >  2:3442804/1‑93 (MQ=255)

TACACCAGATAACCGCCGGTAGTATGCAGCACACCTTTTGGCTTACCGGTAGAACCGGAGGTGTAGAGAATAAACAGCGGATCTTCGGCGTTCATCTCTTCCGCCTGGTGCTGATCGCTCGCTTGCTCAACCAGGTCGTGCCACCACAGGTCGCGCCCTTCCTGCCAGTCAATTTTCCCG  >  NC_000913/4284100‑4284279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`