BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	539,651	A→G	L427L (TTA→TTG)	ybbX →	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	539,651	0	A	G	99.5%	687.5 / NA	212	L427L (TTA→TTG)	ybbX	putative hydrolase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (109/102); minor base C (0/1); total (109/103)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

TGTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCC  >  NC_000913/539561‑539740

tGTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCg                                                                                         >  1:5601869/1‑93 (MQ=255)

 gTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgt                                                                                        >  1:3342823/1‑93 (MQ=255)

  ttCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTCCCATTGGCGCGCGTATCACGAAAACCATCTTGCgtg                                                                                       <  2:795000/93‑1 (MQ=255)

  ttCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtg                                                                                       >  2:2026027/1‑93 (MQ=255)

   tCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtgg                                                                                      <  2:4261685/93‑1 (MQ=255)

     ttACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCgtggtg                                                                                    >  2:5126185/1‑93 (MQ=255)

      tACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtga                                                                                   >  2:3433978/1‑93 (MQ=255)

        ccAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatg                                                                                 >  1:1253346/1‑93 (MQ=255)

        ccAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatg                                                                                 >  1:5275287/1‑93 (MQ=255)

         cAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgt                                                                                >  2:5431163/1‑93 (MQ=255)

            tGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtgat                                                                             >  1:1534332/1‑93 (MQ=255)

            tGACGATCTGGAATATCGCCACAAAGTCAGCCCGCATGTCGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGgtgatgtgat                                                                             >  1:4567676/1‑93 (MQ=255)

              aCGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAttt                                                                           <  1:5659072/93‑1 (MQ=255)

              aCGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGAttt                                                                           >  1:2662542/1‑93 (MQ=255)

               cGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTa                                                                          <  1:873425/93‑1 (MQ=255)

                 aTCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACg                                                                        >  1:785696/1‑93 (MQ=255)

                 aTCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACg                                                                        >  1:2578415/1‑93 (MQ=255)

                  tCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGa                                                                       >  1:5998914/1‑93 (MQ=255)

                  tCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGa                                                                       >  1:5606113/1‑93 (MQ=255)

                  tCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGa                                                                       >  2:4425416/1‑93 (MQ=255)

                   cTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGAc                                                                      <  2:4877626/93‑1 (MQ=255)

                    tGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACa                                                                     >  2:2057876/1‑93 (MQ=255)

                     ggAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAt                                                                    >  1:4653027/1‑93 (MQ=255)

                     ggAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAt                                                                    >  2:1498798/1‑93 (MQ=255)

                     ggAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAt                                                                    >  1:4546648/1‑93 (MQ=255)

                      gAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAtt                                                                   >  2:5265574/1‑93 (MQ=255)

                      gAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACAtt                                                                   <  1:1570931/93‑1 (MQ=255)

                       aaTATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTg                                                                  <  1:323913/93‑1 (MQ=255)

                         tatCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGaa                                                                >  2:4964326/1‑93 (MQ=255)

                          agcGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAAc                                                               <  2:2692930/91‑1 (MQ=255)

                             gCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAgg                                                            <  2:113757/93‑1 (MQ=255)

                             gCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAgg                                                            <  1:5874403/93‑1 (MQ=255)

                             gCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAgg                                                            >  1:4474746/1‑93 (MQ=255)

                              ccACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAggg                                                           >  2:3931614/1‑93 (MQ=255)

                               cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                          >  2:4386207/1‑93 (MQ=255)

                               cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                          <  1:2250177/93‑1 (MQ=255)

                               cacaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGc                                                          >  1:2901025/1‑93 (MQ=255)

                                acaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCt                                                         <  2:3961907/93‑1 (MQ=255)

                                acaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCt                                                         >  2:4546275/1‑93 (MQ=255)

                                acaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCt                                                         >  1:993291/1‑93 (MQ=255)

                                acaAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCt                                                         <  1:1400472/93‑1 (MQ=255)

                                  aaaGTCATCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTc                                                       <  2:3040798/93‑1 (MQ=255)

                                  aaaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTc                                                       >  1:3599510/1‑93 (MQ=255)

                                   aaGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTcc                                                      >  2:1261297/1‑93 (MQ=255)

                                    aGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTccc                                                     <  1:5117432/93‑1 (MQ=255)

                                    aGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTccc                                                     <  1:3655736/93‑1 (MQ=255)

                                       cAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACTAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGt                                                  <  1:4125499/93‑1 (MQ=255)

                                         gCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTg                                                <  2:3545627/93‑1 (MQ=255)

                                         gCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTg                                                <  2:160041/93‑1 (MQ=255)

                                          cccGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgc                                               >  2:2524789/1‑93 (MQ=255)

                                           ccGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgtg                                              >  2:3836427/1‑91 (MQ=255)

                                           ccGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTgcg                                              >  2:827319/1‑93 (MQ=255)

                                               aTGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGa                                          >  2:2464339/1‑93 (MQ=255)

                                                tGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaa                                         >  2:677776/1‑93 (MQ=255)

                                                 gTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaaa                                        <  2:2296218/93‑1 (MQ=255)

                                                 gTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGaaa                                        <  2:1832083/93‑1 (MQ=255)

                                                  ttGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAg                                       <  2:878810/93‑1 (MQ=255)

                                                  ttGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAg                                       <  1:852821/93‑1 (MQ=255)

                                                   tGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAgg                                      <  2:5608772/93‑1 (MQ=255)

                                                     gCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTc                                    <  1:1017602/93‑1 (MQ=255)

                                                      ccGTCCCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCa                                   <  2:1687907/93‑1 (MQ=255)

                                                      ccGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCa                                   >  1:2394348/1‑93 (MQ=255)

                                                      ccGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCa                                   <  1:4842725/93‑1 (MQ=255)

                                                       catACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCaa                                  <  2:2200358/91‑1 (MQ=255)

                                                       cGTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCaa                                  <  1:5415465/93‑1 (MQ=255)

                                                        gTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAt                                 >  2:1411016/1‑93 (MQ=255)

                                                        gTACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAAt                                 <  1:1141296/93‑1 (MQ=255)

                                                         tACCATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAACGGTCAAtt                                >  1:1442251/1‑93 (MQ=255)

                                                           ccATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTa                              <  2:6025483/93‑1 (MQ=255)

                                                           ccATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTa                              >  2:3337818/1‑93 (MQ=255)

                                                            cATTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTAt                             >  2:4136191/1‑93 (MQ=255)

                                                             aTTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGTTCAATTTATc                            >  2:6239655/1‑93 (MQ=255)

                                                             aTTGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATc                            <  2:5496792/93‑1 (MQ=255)

                                                               tGGCGCGCGTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCt                          <  1:3083227/93‑1 (MQ=255)

                                                                   gcgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTaaa                      <  1:4359469/93‑1 (MQ=255)

                                                                   gcgcgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTaaa                      >  1:3959068/1‑93 (MQ=255)

                                                                      cgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACAt                   <  2:3415347/93‑1 (MQ=255)

                                                                      cgTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACAt                   >  1:1592395/1‑93 (MQ=255)

                                                                       gTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACAta                  >  2:2951844/1‑92 (MQ=255)

                                                                       gTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATc                  >  2:5189204/1‑93 (MQ=255)

                                                                       gTATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATc                  >  1:1953998/1‑93 (MQ=255)

                                                                        tATCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATca                 <  1:2040597/93‑1 (MQ=255)

                                                                          tCACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagc               <  1:1473777/93‑1 (MQ=255)

                                                                           cACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagca              >  2:693354/1‑93 (MQ=255)

                                                                           cACGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagca              >  1:5923038/1‑93 (MQ=255)

                                                                            aCGAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATcagcag             <  1:2022603/93‑1 (MQ=255)

                                                                              gAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTa           <  1:210345/93‑1 (MQ=255)

                                                                              gAAAACCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTa           <  2:328353/93‑1 (MQ=255)

                                                                                 aaCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATc        >  2:4472082/1‑93 (MQ=255)

                                                                                  aCCATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCt       <  2:1339114/93‑1 (MQ=255)

                                                                                   ccATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTg      <  1:733473/93‑1 (MQ=255)

                                                                                   ccATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTg      <  1:4172819/93‑1 (MQ=255)

                                                                                    cATCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTgg     >  1:5361202/1‑93 (MQ=255)

                                                                                     aTCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGc    <  1:33860/93‑1 (MQ=255)

                                                                                     aTCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGc    <  1:6240157/93‑1 (MQ=255)

                                                                                     aTCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGc    >  1:1219453/1‑93 (MQ=255)

                                                                                      tCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGcc   >  1:1357555/1‑93 (MQ=255)

                                                                                      tCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGcc   <  2:4838844/93‑1 (MQ=255)

                                                                                      tCTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGcc   >  2:2110924/1‑93 (MQ=255)

                                                                                       cTTGCGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGccc  >  1:5836448/1‑93 (MQ=255)

TGTTCTTACCAATGACGATCTGGAATATCGCCACAAAGTCAGCCCGTATGTTGGCCGTACCATTGGCGCGCGTATCACGAAAACCATCTTACGTGGTGATGTGATTTACGACATTGAACAGGGCTTCCCTGTTGCGCCGAAAGGTCAATTTATCCTTAAACATCAGCAGTAATCTGGCCC  >  NC_000913/539561‑539740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`