BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,368,258	A→G	intergenic (‑268/‑8)	yjeH ← / → mopB	putative transport/GroES

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,368,258	0	A	G	99.6%	865.3 / NA	267	intergenic (‑268/‑8)	yjeH/mopB	putative transport/GroES
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (117/149); total (117/150)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 277 total aligned reads displayed.

GAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCTGA  >  NC_000913/4368168‑4368347

 aaGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGtt                                                                                        >  2:4816996/1‑93 (MQ=255)

  aGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTa                                                                                       <  2:3710340/93‑1 (MQ=255)

   gCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTAt                                                                                      >  1:5170555/1‑93 (MQ=255)

    ccTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATc                                                                                     <  2:3398714/93‑1 (MQ=255)

      tCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCaa                                                                                   >  1:2834154/1‑93 (MQ=255)

        aTCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATg                                                                                 <  2:166003/93‑1 (MQ=255)

         tCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGa                                                                                <  2:2891210/93‑1 (MQ=255)

         tCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGa                                                                                >  2:4660808/1‑93 (MQ=255)

            ccATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAata                                                                             <  1:5601246/93‑1 (MQ=255)

             cATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAatat                                                                            <  2:6032698/93‑1 (MQ=255)

             cATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAatat                                                                            <  1:1874397/93‑1 (MQ=255)

              aTTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATAtt                                                                           <  2:1942803/93‑1 (MQ=255)

               tttCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTc                                                                          >  2:3200539/1‑93 (MQ=255)

                ttCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCg                                                                         <  2:625406/93‑1 (MQ=255)

                 tctcTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGt                                                                        >  1:1686207/1‑93 (MQ=255)

                 tcgctGGTCACCAGCCGGGAAACCACGTAAGCTCCGGAGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGt                                                                        <  1:684457/90‑1 (MQ=255)

                   tctGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTcc                                                                      <  2:2647001/93‑1 (MQ=255)

                    ctGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCa                                                                     <  1:1378880/93‑1 (MQ=255)

                     tGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCAt                                                                    >  2:2661149/1‑93 (MQ=255)

                     tGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCAt                                                                    <  2:4688219/93‑1 (MQ=255)

                      ggTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCAtt                                                                   >  1:5738028/1‑93 (MQ=255)

                      ggTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCAtt                                                                   <  1:5494071/93‑1 (MQ=255)

                      ggTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCAtt                                                                   <  1:1770455/93‑1 (MQ=255)

                       gTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTg                                                                  >  2:2101461/1‑93 (MQ=255)

                        tCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGc                                                                 >  1:3104331/1‑93 (MQ=255)

                         cACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCa                                                                <  2:5968488/93‑1 (MQ=255)

                             aGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGAt                                                            <  1:3742564/93‑1 (MQ=255)

                             aGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGAt                                                            <  2:6191415/93‑1 (MQ=255)

                             aGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGAt                                                            <  1:264819/93‑1 (MQ=255)

                             aGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGAt                                                            <  1:1659406/93‑1 (MQ=255)

                               ccGGTAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATcg                                                          <  1:2503287/93‑1 (MQ=255)

                               ccGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATcg                                                          <  2:2940612/93‑1 (MQ=255)

                               ccGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATcc                                                          <  2:218525/93‑2 (MQ=255)

                                 gggAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATcgcg                                                        >  1:6169760/1‑93 (MQ=255)

                                  ggAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGt                                                       >  1:1334226/1‑93 (MQ=255)

                                    aaaCCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTgt                                                     >  1:1539782/1‑92 (MQ=255)

                                    aaaCCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGa                                                     <  2:4065135/93‑1 (MQ=255)

                                     aaCCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGAt                                                    >  1:1088306/1‑93 (MQ=255)

                                     aaCCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGAt                                                    <  1:527562/93‑1 (MQ=255)

                                     aaCCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGAt                                                    <  1:4160192/93‑1 (MQ=255)

                                       ccACGTAAGCTCCGGCGTCACCCATAACAGATTCGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCg                                                  <  2:5777806/93‑1 (MQ=255)

                                       ccACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCg                                                  <  1:4043195/93‑1 (MQ=255)

                                         aCGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTc                                                >  1:4598397/1‑93 (MQ=255)

                                         aCGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTc                                                >  2:4961753/1‑93 (MQ=255)

                                          cGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCa                                               <  2:452564/93‑1 (MQ=255)

                                          cGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCa                                               <  1:1264247/93‑1 (MQ=255)

                                           gTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCaa                                              <  1:5968135/93‑1 (MQ=255)

                                           gTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCaa                                              <  1:2398641/93‑1 (MQ=255)

                                            tAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAg                                             >  2:5577751/1‑93 (MQ=255)

                                              aGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCg                                           <  1:228591/93‑1 (MQ=255)

                                              aGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCg                                           >  1:1989831/1‑93 (MQ=255)

                                                 tCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTaa                                        >  1:5292497/1‑93 (MQ=255)

                                                 tCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTaa                                        >  1:2728371/1‑93 (MQ=255)

                                                  ccGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTaaa                                       <  1:4479864/93‑1 (MQ=255)

                                                  ccGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTaaa                                       <  2:5648279/93‑1 (MQ=255)

                                                    gtcGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAaaga                                     <  2:74901/91‑1 (MQ=255)

                                                      cGTCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAaagaag                                   <  1:1242808/93‑1 (MQ=255)

                                                        tCACCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGtt                                 <  2:1342705/93‑1 (MQ=255)

                                                          aCCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGa                               >  2:3355700/1‑93 (MQ=255)

                                                          aCCCATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGa                               >  2:5405180/1‑93 (MQ=255)

                                                            ccATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGaaa                             <  2:328313/93‑1 (MQ=255)

                                                             cATAACAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAAc                            <  1:3942417/93‑1 (MQ=255)

                                                              aTAACAGATACGGACTTTCGCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACt                           <  2:4280672/93‑1 (MQ=255)

                                                                aaCAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTaa                         >  2:275138/1‑93 (MQ=255)

                                                                aaCAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTaa                         <  1:5566407/93‑1 (MQ=255)

                                                                aaCAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTaa                         >  1:6019146/1‑93 (MQ=255)

                                                                 aCAGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTaaa                        >  2:2495800/1‑93 (MQ=255)

                                                                   aGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATc                      <  1:6256175/93‑1 (MQ=255)

                                                                   aGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATc                      <  1:4741760/93‑1 (MQ=255)

                                                                   aGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATc                      <  2:56261/93‑1 (MQ=255)

                                                                   aGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATc                      <  1:3347775/93‑1 (MQ=255)

                                                                   aGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATc                      >  2:361373/1‑93 (MQ=255)

                                                                   aGATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATc                      <  1:3016734/93‑1 (MQ=255)

                                                                    gATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATct                     <  1:3800209/93‑1 (MQ=255)

                                                                    gATACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATct                     <  2:3526403/93‑1 (MQ=255)

                                                                      tACGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATctgc                   <  2:5179589/93‑1 (MQ=255)

                                                                       aCGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATctgct                  >  2:879068/1‑93 (MQ=255)

                                                                       aCGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATctgct                  >  1:4805325/1‑93 (MQ=255)

                                                                       aCGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATctgct                  <  1:5769756/93‑1 (MQ=255)

                                                                        cGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATctgctg                 <  1:759042/93‑1 (MQ=255)

                                                                        cGGACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATctgctg                 <  2:2569315/93‑1 (MQ=255)

                                                                          gACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTggg               >  1:4620759/1‑92 (MQ=255)

                                                                          gACTTTCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTggc               <  1:1592373/93‑1 (MQ=255)

                                                                             tttCTCAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTggcggc            >  1:4935614/1‑93 (MQ=255)

                                                                               tctcAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCAt          >  1:6146735/1‑93 (MQ=255)

                                                                                ctcAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATc         >  1:3566214/1‑93 (MQ=255)

                                                                                ctcAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATc         >  2:5260063/1‑93 (MQ=255)

                                                                                  cAAAGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGt       >  2:1463915/1‑93 (MQ=255)

                                                                                  cAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGt       <  2:2242918/93‑1 (MQ=255)

                                                                                   aaaGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGtt      >  2:4416490/1‑93 (MQ=255)

                                                                                   aaaGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGtt      <  2:1008243/93‑1 (MQ=255)

                                                                                    aaGGAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTc     <  1:2172813/93‑1 (MQ=255)

                                                                                     aggaggGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCt    >  1:436439/1‑93 (MQ=255)

                                                                                     aggaggGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCt    >  1:4639827/1‑93 (MQ=255)

                                                                                     aggaggGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCt    <  2:3116857/93‑1 (MQ=255)

                                                                                     aggaggGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCt    >  2:1097437/1‑93 (MQ=255)

                                                                                     aggaggGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCt    <  1:5791262/93‑1 (MQ=255)

                                                                                     aggaggGTTATCAATGAATATTCGTCCATTGCATGATCGCCTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCt    >  2:5533336/1‑93 (MQ=255)

                                                                                      ggAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCTg   >  2:2394637/1‑93 (MQ=255)

                                                                                      ggAGGGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCTg   >  2:1864947/1‑93 (MQ=255)

GAAGCCTCATCCCCATTTCTCTGGTCACCAGCCGGGAAACCACGTAAGCTCCGGCGTCACCCATAACAGATACGGACTTTCTCAAAGGAGAGTTATCAATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAACTAAATCTGCTGGCGGCATCGTTCTGA  >  NC_000913/4368168‑4368347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`