BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,435,689	C→T	D135D (GAC→GAT)	ytfI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,435,689	0	C	T	100.0%	869.7 / NA	230	D135D (GAC→GAT)	ytfI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (122/108); total (122/108)

Only 100 of 230 total aligned reads displayed.

TGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG  >  NC_000913/4435602‑4435778

 gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTg                                                                                      <  1:5969670/93‑1 (MQ=255)

 gAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTg                                                                                      <  2:4744360/93‑1 (MQ=255)

   aTACGAAGAAATACCCATAACAATAAACGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTa                                                                                    <  1:5551074/93‑1 (MQ=255)

    tACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTAt                                                                                   <  1:2604009/93‑1 (MQ=255)

     aCGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATg                                                                                  >  2:5869251/1‑93 (MQ=255)

         agaaATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTg                                                                              <  1:4253953/93‑1 (MQ=255)

            aaTACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGa                                                                           <  2:1228693/93‑1 (MQ=255)

              tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGata                                                                         >  1:1422085/1‑93 (MQ=255)

              tACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGata                                                                         <  2:5760521/93‑1 (MQ=255)

                 ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTc                                                                      <  2:5722360/93‑1 (MQ=255)

                 ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTc                                                                      <  2:4847926/93‑1 (MQ=255)

                 ccATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTc                                                                      <  2:3173410/93‑1 (MQ=255)

                 ccATAACAATAAATGAATATAACTCATTCATGGATTCCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTc                                                                      <  2:4936625/93‑1 (MQ=255)

                  cATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCt                                                                     >  2:1549674/1‑93 (MQ=255)

                   aTAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTa                                                                    >  2:4601651/1‑93 (MQ=255)

                   aTAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATACTCTa                                                                    >  2:3017546/1‑93 (MQ=255)

                    tAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGAGATATTCTAc                                                                   >  1:4423418/1‑93 (MQ=255)

                     aaCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACa                                                                  <  2:3867839/93‑1 (MQ=255)

                      aCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCTTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAg                                                                 <  1:4436106/93‑1 (MQ=255)

                      aCAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAg                                                                 >  2:2489339/1‑93 (MQ=255)

                        aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaa                                                               >  1:984677/1‑93 (MQ=255)

                        aaTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaa                                                               >  2:2928708/1‑93 (MQ=255)

                         aTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaa                                                              <  1:5519224/93‑1 (MQ=255)

                         aTAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaa                                                              >  2:1455386/1‑93 (MQ=255)

                          tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                             <  1:307915/93‑1 (MQ=255)

                          tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                             >  1:1847354/1‑93 (MQ=255)

                          tAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaa                                                             >  2:2185641/1‑93 (MQ=255)

                           aaaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaa                                                            >  1:6055644/1‑93 (MQ=255)

                            aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                           >  2:532730/1‑93 (MQ=255)

                            aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                           <  2:5317290/93‑1 (MQ=255)

                            aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                           <  1:2852877/93‑1 (MQ=255)

                            aaTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGaaaaaa                                                           >  2:4970298/1‑93 (MQ=255)

                             aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                          <  2:5977119/93‑1 (MQ=255)

                             aTGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAAc                                                          >  2:4555223/1‑93 (MQ=255)

                              tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                                         >  2:5510981/1‑93 (MQ=255)

                              tGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACa                                                         <  2:1110530/93‑1 (MQ=255)

                               gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAGAACAg                                                        >  2:2566897/1‑93 (MQ=255)

                               gAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAg                                                        <  2:5671121/93‑1 (MQ=255)

                                 atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                                      <  1:324567/93‑1 (MQ=255)

                                 atatAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTc                                                      >  2:236205/1‑93 (MQ=255)

                                  tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                                     >  2:5117117/1‑93 (MQ=255)

                                  tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                                     >  1:414832/1‑93 (MQ=255)

                                  tataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCt                                                     <  1:2386505/93‑1 (MQ=255)

                                   ataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTg                                                    <  1:4834974/93‑1 (MQ=255)

                                   ataACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTg                                                    <  1:3263487/93‑1 (MQ=255)

                                    taACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                                   <  1:3055930/93‑1 (MQ=255)

                                    taACTCATTCATGAATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGa                                                   <  1:4188947/93‑1 (MQ=255)

                                     aaCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAc                                                  >  2:5910998/1‑93 (MQ=255)

                                     aaCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGAc                                                  >  1:3681176/1‑93 (MQ=255)

                                      aCTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACa                                                 >  2:2078161/1‑93 (MQ=255)

                                       cTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAt                                                >  1:5996705/1‑93 (MQ=255)

                                       cTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACAt                                                <  1:1866143/93‑1 (MQ=255)

                                        tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                               >  2:6207987/1‑93 (MQ=255)

                                        tcattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATa                                               >  2:4154484/1‑93 (MQ=255)

                                         cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACGGAAAAAACAGTCTGACATAc                                              >  2:912880/1‑93 (MQ=255)

                                         cattcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAc                                              >  1:64103/1‑93 (MQ=255)

                                          attcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATAcc                                             >  1:513764/1‑93 (MQ=255)

                                           ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                            >  2:5223498/1‑93 (MQ=255)

                                           ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                            <  2:1879282/93‑1 (MQ=255)

                                           ttcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCg                                            >  2:2904132/1‑93 (MQ=255)

                                            tcatGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGa                                           >  1:5729736/1‑93 (MQ=255)

                                             catGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa                                          >  2:5160297/1‑93 (MQ=255)

                                             catGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaa                                          >  1:2331991/1‑93 (MQ=255)

                                              atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                                         <  1:4043266/93‑1 (MQ=255)

                                              atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                                         <  1:4620203/93‑1 (MQ=255)

                                              atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                                         >  1:4794122/1‑93 (MQ=255)

                                              atGGATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGaaa                                         >  2:5050424/1‑93 (MQ=255)

                                                ggATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAg                                       <  2:2287889/93‑1 (MQ=255)

                                                 gATTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAgg                                      >  1:2770620/1‑93 (MQ=255)

                                                  aTTTCAAAAACCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGa                                     >  2:3146199/1‑93 (MQ=255)

                                                       aaaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                                >  2:2660417/1‑93 (MQ=255)

                                                       aaaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTaaa                                <  2:3711428/93‑1 (MQ=255)

                                                        aaaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAg                               >  2:6132345/1‑93 (MQ=255)

                                                         aaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAgg                              >  1:883053/1‑93 (MQ=255)

                                                         aaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAgg                              >  2:5836842/1‑93 (MQ=255)

                                                         aaaCCGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAgg                              <  2:5291732/93‑1 (MQ=255)

                                                             cGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                          <  1:5959588/93‑1 (MQ=255)

                                                             cGTGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTtat                          <  1:3654633/93‑1 (MQ=255)

                                                               tGAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTatat                        <  1:1302765/93‑1 (MQ=255)

                                                                gAATTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATAtt                       >  1:4486228/1‑93 (MQ=255)

                                                                  aTTTCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCa                     <  2:661822/93‑1 (MQ=255)

                                                                    ttCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCAcc                   <  2:2226247/93‑1 (MQ=255)

                                                                     tCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa                  <  1:1983220/93‑1 (MQ=255)

                                                                     tCCGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCa                  >  2:814189/1‑93 (MQ=255)

                                                                      ccGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAg                 <  1:5101900/93‑1 (MQ=255)

                                                                      ccGTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAg                 <  1:1144820/93‑1 (MQ=255)

                                                                        gTTAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGc               <  1:3325381/93‑1 (MQ=255)

                                                                         ttAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCg              >  2:3325845/1‑93 (MQ=255)

                                                                          tAGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGa             >  2:4191201/1‑93 (MQ=255)

                                                                           aGAACAAAACAGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGAt            <  2:2388298/93‑1 (MQ=255)

                                                                              acaaaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTgg         <  1:667772/90‑1 (MQ=255)

                                                                               caaaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGt        <  1:5073451/91‑1 (MQ=255)

                                                                               caaaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGt        <  2:874212/91‑1 (MQ=255)

                                                                                aaaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTg       >  2:3565154/2‑93 (MQ=255)

                                                                                aaaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTg       >  2:3514298/2‑93 (MQ=255)

                                                                                 aaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGa      <  1:3599995/93‑1 (MQ=255)

                                                                                 aaacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGa      <  1:5416068/93‑1 (MQ=255)

                                                                                  aacaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGAc     >  1:1080479/1‑93 (MQ=255)

                                                                                   acaGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACg    >  2:1928064/1‑93 (MQ=255)

                                                                                     aGATGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACgtg  >  1:5025754/1‑93 (MQ=255)

TGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAAC‑AAACAGACGATTGTATGCCTGGGATATTCTACAGAAAAAACAGTCTGACATACCGAAAAGGATTAAAGGTTATATTCACCAGGCGATTGGTGACGTG  >  NC_000913/4435602‑4435778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`