BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,441,383	T→C	S475S (AGT→AGC)	ytfM →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,441,383	0	T	C	100.0%	697.5 / NA	217	S475S (AGT→AGC)	ytfM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (100/117); total (100/117)

Only 100 of 224 total aligned reads displayed.

GTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGG  >  NC_000913/4441294‑4441471

gTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCAtt                                                                                       <  1:2985054/93‑1 (MQ=255)

gTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGGTTCTTCGCCGGGGGCGACCGCAGCAtt                                                                                       >  1:4225196/1‑93 (MQ=255)

 ttACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTc                                                                                      <  2:2257979/93‑1 (MQ=255)

 ttACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTc                                                                                      <  1:3657980/93‑1 (MQ=255)

   acacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGt                                                                                    <  1:1004538/93‑1 (MQ=255)

   acacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGt                                                                                    <  1:2553040/93‑1 (MQ=255)

   acacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGt                                                                                    >  2:615142/1‑93 (MQ=255)

    cacGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTg                                                                                   >  2:5351270/1‑93 (MQ=255)

         ggCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtac                                                                              <  2:2915952/93‑1 (MQ=255)

            aCGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaa                                                                           >  1:4941425/1‑93 (MQ=255)

            aCGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaa                                                                           <  2:1366494/93‑1 (MQ=255)

                tgggctggATTGAAACCGGTGATTTCGACCAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaataca                                                                       >  1:2775726/1‑93 (MQ=255)

                tgggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaataca                                                                       >  1:834146/1‑93 (MQ=255)

                 gggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaatacaa                                                                      >  2:1079620/1‑93 (MQ=255)

                  ggctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCtacaaatacaaa                                                                     >  1:4800263/1‑93 (MQ=255)

                    ctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATc                                                                   <  2:3643766/93‑1 (MQ=255)

                    ctggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATc                                                                   <  2:3286590/93‑1 (MQ=255)

                     tggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCt                                                                  >  1:1274251/1‑93 (MQ=255)

                     tggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCt                                                                  <  1:2143831/93‑1 (MQ=255)

                      ggATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTa                                                                 <  2:845652/93‑1 (MQ=255)

                       gATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTAt                                                                <  2:4155890/93‑1 (MQ=255)

                         atGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCg                                                              <  2:4618824/92‑1 (MQ=255)

                          tGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGc                                                             <  2:6116552/93‑1 (MQ=255)

                            aaaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTc                                                           <  1:5507176/93‑1 (MQ=255)

                             aaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTcc                                                          >  2:3226922/1‑93 (MQ=255)

                             aaCCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTcc                                                          >  2:241000/1‑93 (MQ=255)

                               ccGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGa                                                        >  1:972806/1‑93 (MQ=255)

                               acGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGa                                                        <  1:4256847/92‑1 (MQ=255)

                                cGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATATATGGCTCCGaa                                                       >  2:3735500/1‑93 (MQ=255)

                                 ggTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGaaa                                                      <  2:2190955/93‑1 (MQ=255)

                                  gTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAt                                                     >  2:1153478/1‑93 (MQ=255)

                                  gTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAAt                                                     <  1:1904388/93‑1 (MQ=255)

                                   tGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATa                                                    <  1:6239847/93‑1 (MQ=255)

                                   tGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATa                                                    <  1:4586508/93‑1 (MQ=255)

                                    gATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATAc                                                   <  1:1357717/93‑1 (MQ=255)

                                       ttCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGcc                                                <  2:3281052/93‑1 (MQ=255)

                                        tCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCa                                               <  1:2429312/93‑1 (MQ=255)

                                         cGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCaa                                              <  1:4074297/93‑1 (MQ=255)

                                          gACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAAc                                             <  1:2464902/93‑1 (MQ=255)

                                            cAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACgg                                           >  1:336356/1‑93 (MQ=255)

                                            cAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACgg                                           >  1:5650303/1‑93 (MQ=255)

                                            cAAAGTACCGCCGGATCTGCGTTTCTGCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACgg                                           <  1:1389050/93‑1 (MQ=255)

                                             aaaGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACggg                                          >  1:1836200/1‑92 (MQ=255)

                                             aaaGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGACAACGGt                                          >  1:1089790/1‑93 (MQ=255)

                                              aaGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTg                                         <  1:3909166/93‑1 (MQ=255)

                                               aGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGa                                        >  1:2068651/1‑93 (MQ=255)

                                                gTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGAc                                       >  2:4106764/1‑93 (MQ=255)

                                                  aCCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCt                                     <  2:5038819/93‑1 (MQ=255)

                                                    cgccgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGa                                   >  2:5139384/1‑93 (MQ=255)

                                                     gccgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaa                                  <  2:191087/93‑1 (MQ=255)

                                                      ccgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGaaa                                 <  1:3734482/93‑1 (MQ=255)

                                                       cgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAg                                >  1:5436246/1‑93 (MQ=255)

                                                       cgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAg                                <  2:4566487/93‑1 (MQ=255)

                                                       cgGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTACCGCTCCGAAATACGCCAACGGTGACCTGAAAg                                <  2:732681/93‑1 (MQ=255)

                                                        ggATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCCACGGTGACCTGAAAgg                               >  2:1697279/1‑93 (MQ=255)

                                                        ggATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAgg                               >  1:2688234/1‑93 (MQ=255)

                                                         gATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                              <  1:5184798/93‑1 (MQ=255)

                                                         gATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                              >  1:5778879/1‑93 (MQ=255)

                                                         gATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                              >  1:2249394/1‑93 (MQ=255)

                                                         gATCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAggg                              >  2:4248049/1‑93 (MQ=255)

                                                           tCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGc                            >  1:2847441/1‑93 (MQ=255)

                                                           tCTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGc                            >  2:3022001/1‑93 (MQ=255)

                                                            cTGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGcc                           <  1:5691097/93‑1 (MQ=255)

                                                             tGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCt                          >  2:631762/1‑93 (MQ=255)

                                                             tGCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCt                          >  2:1460184/1‑93 (MQ=255)

                                                             tGCGTTTCTTCACCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCt                          <  2:2506831/93‑1 (MQ=255)

                                                              gCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTc                         >  2:2350047/1‑93 (MQ=255)

                                                              gCGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTc                         <  1:4504702/93‑1 (MQ=255)

                                                               cGTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCg                        >  2:4935122/1‑93 (MQ=255)

                                                                gTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGa                       >  1:6054760/1‑93 (MQ=255)

                                                                gTTTCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGa                       >  2:1895954/1‑93 (MQ=255)

                                                                 tttCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGaa                      >  2:3118173/1‑93 (MQ=255)

                                                                 tttCTTCGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGaa                      >  2:2754445/1‑93 (MQ=255)

                                                                  ttcttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAg                     <  2:5193346/93‑1 (MQ=255)

                                                                   tcttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGCAGt                    >  2:549309/1‑93 (MQ=255)

                                                                   tcttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGt                    >  2:2642114/1‑93 (MQ=255)

                                                                   tcttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGt                    <  2:2850716/93‑1 (MQ=255)

                                                                   tcttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGt                    <  2:1590801/93‑1 (MQ=255)

                                                                    cttcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGtt                   <  2:5927683/93‑1 (MQ=255)

                                                                      tcGCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGa                 <  1:4600235/93‑1 (MQ=255)

                                                                        gCCGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATTTATCGCTCCGAAATACGCCAACGGGGACCTGAAAGGGGCCTCGAAGTTGATa               >  2:6144425/1‑93 (MQ=255)

                                                                         ccGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATaa              <  1:1129122/93‑1 (MQ=255)

                                                                          cGGGGGCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAc             <  1:5840231/93‑1 (MQ=255)

                                                                           gggggCGACCGCAGCATTCGTGGCTACAAATACTAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAcc            >  1:908963/1‑93 (MQ=255)

                                                                           gggggCGACCGCAGCATGCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAAcc            <  2:4488966/93‑1 (MQ=255)

                                                                            ggggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCg           <  2:6012093/93‑1 (MQ=255)

                                                                            ggggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCg           >  1:4351928/1‑93 (MQ=255)

                                                                             gggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCgg          <  1:5904245/93‑1 (MQ=255)

                                                                              ggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGa         <  2:4515525/93‑1 (MQ=255)

                                                                              ggCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGa         <  1:1498038/93‑1 (MQ=255)

                                                                               gCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGAt        <  2:674201/93‑1 (MQ=255)

                                                                               gCGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGAt        <  2:891529/93‑1 (MQ=255)

                                                                                cGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATc       <  2:2684364/93‑1 (MQ=255)

                                                                                cGACCGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATc       <  1:5115762/93‑1 (MQ=255)

                                                                                    cGCAGCATTCGTGGCTACAAATACAAATGTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTg   >  2:2052328/1‑93 (MQ=255)

                                                                                    cGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTg   >  1:2083922/1‑93 (MQ=255)

                                                                                    cGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTg   <  1:4711292/93‑1 (MQ=255)

                                                                                    cGCAGCATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGGTGATAACCGGATCGCTg   >  2:2308110/1‑93 (MQ=255)

                                                                                     gcagcaTTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTgg  >  1:1238803/1‑93 (MQ=255)

                                                                                     gcagcaTTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTgg  >  1:5123140/1‑93 (MQ=255)

GTTACACGCGGCACGCTGGGCTGGATTGAAACCGGTGATTTCGACAAAGTACCGCCGGATCTGCGTTTCTTCGCCGGGGGCGACCGCAGTATTCGTGGCTACAAATACAAATCTATCGCTCCGAAATACGCCAACGGTGACCTGAAAGGGGCCTCGAAGTTGATAACCGGATCGCTGG  >  NC_000913/4441294‑4441471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`