BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,544,170	T→C	I502I (ATT→ATC)	fimD →	FimD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,544,170	0	T	C	99.6%	793.0 / NA	243	I502I (ATT→ATC)	fimD	FimD
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (131/111); minor base A (1/0); total (132/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAC  >  NC_000913/4544080‑4544259

gAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGa                                                                                         >  2:891017/1‑93 (MQ=255)

 aaTATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGaa                                                                                        >  1:5271471/1‑93 (MQ=255)

  atatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGaaa                                                                                       >  2:3919011/1‑93 (MQ=255)

   tatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGCATGGCTAGAACATCGAAac                                                                                      >  1:2569209/1‑93 (MQ=255)

   tatTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAac                                                                                      <  2:168428/93‑1 (MQ=255)

     ttCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAacac                                                                                    >  2:714634/1‑93 (MQ=255)

       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATTGCTACAACATCGAAACACAg                                                                                  >  2:6294717/1‑93 (MQ=255)

       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAg                                                                                  >  1:503744/1‑93 (MQ=255)

       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAg                                                                                  <  1:1419816/93‑1 (MQ=255)

       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAg                                                                                  <  2:1109358/93‑1 (MQ=255)

       cAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAg                                                                                  <  1:2475304/93‑1 (MQ=255)

        aGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAgg                                                                                 <  2:4876477/93‑1 (MQ=255)

         gTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGa                                                                                >  1:4630638/1‑93 (MQ=255)

         gTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGa                                                                                <  2:1901434/93‑1 (MQ=255)

         gTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGa                                                                                >  2:3977773/1‑93 (MQ=255)

           tAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACg                                                                              <  1:2615883/93‑1 (MQ=255)

            aGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACgg                                                                             >  1:3765361/1‑93 (MQ=255)

            aGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACgg                                                                             >  1:4150033/1‑93 (MQ=255)

             gTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGa                                                                            <  2:1923080/93‑1 (MQ=255)

             gTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGa                                                                            <  2:4102798/93‑1 (MQ=255)

               tggTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGt                                                                          <  1:703694/92‑1 (MQ=255)

                  ttACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTAt                                                                       >  2:2616114/1‑93 (MQ=255)

                    aCCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTc                                                                     >  1:4107422/1‑93 (MQ=255)

                     ccGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCa                                                                    <  2:2347104/93‑1 (MQ=255)

                      cGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAg                                                                   <  2:3306565/93‑1 (MQ=255)

                       gTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAAGACGGAGTTATTCAgg                                                                  >  2:5826228/1‑93 (MQ=255)

                        ttATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGt                                                                 <  1:1233887/93‑1 (MQ=255)

                         tATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGtt                                                                >  1:1610247/1‑93 (MQ=255)

                           ttCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTaa                                                              <  1:1000714/93‑1 (MQ=255)

                           ttCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTaa                                                              >  1:5159406/1‑93 (MQ=255)

                             cGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGc                                                            >  1:5426609/1‑93 (MQ=255)

                                ccAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGa                                                         >  1:1486542/1‑93 (MQ=255)

                                  aGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACTGAGGTATTCAGGTTAAGCCGaaa                                                       >  2:2199546/1‑93 (MQ=255)

                                  aGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGaaa                                                       <  2:918865/93‑1 (MQ=255)

                                   gCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAt                                                      <  1:4732050/93‑1 (MQ=255)

                                   gCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAAt                                                      >  1:4073989/1‑93 (MQ=255)

                                    cGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGaaact                                                     >  1:2011336/1‑91 (MQ=255)

                                     ggATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTc                                                    >  1:4933701/1‑93 (MQ=255)

                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  <  1:2132126/93‑1 (MQ=255)

                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  >  1:5594838/1‑93 (MQ=255)

                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  <  1:2736070/93‑1 (MQ=255)

                                       atatTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCAc                                                  >  2:1418805/1‑93 (MQ=255)

                                          ttttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGa                                               <  1:5850160/93‑1 (MQ=255)

                                          ttttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATAGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTTACTGa                                               >  2:3072066/1‑93 (MQ=255)

                                           tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAc                                              <  1:1091248/93‑1 (MQ=255)

                                           tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAc                                              >  2:4606534/1‑93 (MQ=255)

                                           tttAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGAc                                              <  1:5323577/93‑1 (MQ=255)

                                                tttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACATAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTa                                         >  2:6215975/1‑93 (MQ=255)

                                                tttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTa                                         >  2:1741607/1‑93 (MQ=255)

                                                 ttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTAc                                        <  2:1990806/93‑1 (MQ=255)

                                                 ttCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTAc                                        <  1:3315294/93‑1 (MQ=255)

                                                  tCGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACa                                       >  2:4414785/1‑93 (MQ=255)

                                                   cGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACaa                                      >  1:2285737/1‑93 (MQ=255)

                                                   cGCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACaa                                      <  2:1770692/93‑1 (MQ=255)

                                                    gCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAc                                     <  1:907913/93‑1 (MQ=255)

                                                    gCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAc                                     <  1:3777888/93‑1 (MQ=255)

                                                    gCTGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAc                                     <  2:5001280/93‑1 (MQ=255)

                                                      tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                   <  2:3738129/93‑1 (MQ=255)

                                                      tGATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCt                                   <  2:4745106/93‑1 (MQ=255)

                                                       gATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTc                                  >  1:3027296/1‑93 (MQ=255)

                                                       gATACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTc                                  <  2:1291071/93‑1 (MQ=255)

                                                         tACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGc                                <  2:2949021/93‑1 (MQ=255)

                                                         tACAACATACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGc                                >  1:1840192/1‑93 (MQ=255)

                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             >  1:1851969/1‑93 (MQ=255)

                                                            aacaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTta                             >  1:6247339/1‑93 (MQ=255)

                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            <  2:5773018/93‑1 (MQ=255)

                                                             acaTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtat                            >  1:1712686/1‑93 (MQ=255)

                                                              caTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtata                           <  2:3694376/93‑1 (MQ=255)

                                                              caTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGCTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTtata                           >  2:2862303/1‑93 (MQ=255)

                                                               aTACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATaa                          >  2:181397/1‑93 (MQ=255)

                                                                tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                         >  2:4348753/1‑93 (MQ=255)

                                                                tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                         >  2:1050063/1‑93 (MQ=255)

                                                                tACAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGGTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAAc                         >  2:5407993/1‑93 (MQ=255)

                                                                 aCAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACa                        >  1:4888072/1‑93 (MQ=255)

                                                                  cAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaa                       >  1:2528900/1‑93 (MQ=255)

                                                                  cAGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaa                       >  1:1957768/1‑93 (MQ=255)

                                                                   aGTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACaaa                      <  1:973225/93‑1 (MQ=255)

                                                                    gTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAc                     >  1:3908831/1‑93 (MQ=255)

                                                                    gTCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAc                     <  2:5537958/93‑1 (MQ=255)

                                                                     tCGAATGAATGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcg                    <  1:421968/93‑1 (MQ=255)

                                                                       gaatgaatGGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAAcgcg                  <  2:1565183/93‑1 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  2:3456183/1‑93 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  1:2214103/1‑93 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  2:4201107/1‑93 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 <  2:1253733/93‑1 (MQ=255)

                                                                        aatgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCgg                 >  1:2986521/1‑93 (MQ=255)

                                                                         atgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCggg                <  2:3418645/93‑1 (MQ=255)

                                                                         atgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCggg                <  2:5068327/93‑1 (MQ=255)

                                                                         atgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAAACTCGCTTATAACAAACGCggg                <  2:4561716/93‑1 (MQ=255)

                                                                          tgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGa               >  1:6160798/1‑93 (MQ=255)

                                                                          tgaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGa               >  2:5740334/1‑93 (MQ=255)

                                                                           gaatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGaa              <  2:704515/93‑1 (MQ=255)

                                                                            aatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGaaa             <  1:4561292/93‑1 (MQ=255)

                                                                            aatgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGaaa             <  1:3628108/93‑1 (MQ=255)

                                                                              tgGCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAAtt           <  2:2625855/93‑1 (MQ=255)

                                                                                gCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAc         <  2:2834625/93‑1 (MQ=255)

                                                                                gCTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTAc         <  2:375040/93‑1 (MQ=255)

                                                                                 cTACAACATCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACa        >  1:2937399/1‑93 (MQ=255)

                                                                                       caTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAc  >  2:2796333/1‑93 (MQ=255)

                                                                                       caTCGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAc  <  1:5672467/93‑1 (MQ=255)

GAATATTCAGTTAGTGGGTTACCGTTATTCGACCAGCGGATATTTTAATTTCGCTGATACAACATACAGTCGAATGAATGGCTACAACATTGAAACACAGGACGGAGTTATTCAGGTTAAGCCGAAATTCACCGACTATTACAACCTCGCTTATAACAAACGCGGGAAATTACAACTCAC  >  NC_000913/4544080‑4544259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`