BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,785	A→G	noncoding (71/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,785	0	A	G	98.8%	489.2 / ‑4.5	176	noncoding (71/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (97/74); total (98/78)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 199 total aligned reads displayed.

CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603697‑4603874

ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTg                                                                                       >  1:3159587/1‑93 (MQ=255)

     aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATAGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAAcc                                                                                   >  1:1741595/1‑93 (MQ=37)

      gCTAGCGCGTCTACCAATTCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAAcccc                                                                                 <  1:1662271/93‑1 (MQ=255)

      gCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAAccc                                                                                  <  2:1490658/93‑1 (MQ=38)

       cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAAcccc                                                                                 <  1:4618449/93‑1 (MQ=38)

       cTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAAcccc                                                                                 >  1:4103334/1‑93 (MQ=255)

        tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCa                                                                               <  2:6117242/93‑1 (MQ=255)

         aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGG‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCa                                                                               <  2:1531837/93‑1 (MQ=38)

          gcgcGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCAc                                                                              >  2:2748960/1‑93 (MQ=255)

           cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTGGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACg                                                                             <  2:5012346/93‑1 (MQ=38)

            gcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGt                                                                            >  1:3551001/1‑93 (MQ=255)

            ctgTCTACCAATTCCGCCACCTTGACACAGTCATCTTACTTTTTTTGATACCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTc                                                                           <  2:1238115/91‑1 (MQ=37)

            ccgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTc                                                                           <  2:4676407/92‑1 (MQ=255)

             cgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGGGCTTGAACCCCCACGTc                                                                           <  2:2932993/93‑1 (MQ=255)

              gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTcc                                                                          >  1:5885707/1‑93 (MQ=255)

              gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTcc                                                                          >  1:2700141/1‑93 (MQ=255)

              gTCTACCAATCCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCCGGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTcc                                                                          <  2:4797330/93‑1 (MQ=37)

                cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGG‑‑CTTGAACCCCCACGTCCGTaa                                                                      >  1:4125906/1‑93 (MQ=255)

                  aCCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTaa                                                                      >  2:3071124/1‑93 (MQ=255)

                   ccAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATGTCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAg                                                                     >  1:4434944/1‑93 (MQ=37)

                   ccAAGTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGGG‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAg                                                                     <  2:5928417/93‑1 (MQ=37)

                    cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAGGGGGGGGGGG‑CTTGAACCCCCACGGCCCTAAg                                                                     >  1:2304863/1‑93 (MQ=38)

                    cAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCCCGTCCGTAAGa                                                                    >  1:4292414/1‑93 (MQ=38)

                       ttCCGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAaca                                                                 <  1:1483157/93‑1 (MQ=37)

                       ttCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATACCGCCTCGTTTGGG‑GG‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAaca                                                                 <  2:5719590/93‑1 (MQ=37)

                          cGCCCCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTGGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTa                                                              <  1:5742140/93‑1 (MQ=25)

                           gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAac                                                            >  1:2903099/1‑93 (MQ=255)

                             cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAAGACTAacc                                                           >  1:1255363/1‑92 (MQ=37)

                             cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACCCTaaac                                                           >  2:3983699/1‑91 (MQ=37)

                             cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAacac                                                          >  2:618477/1‑93 (MQ=255)

                               ccTTCGCACATTCATCTTATTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                         <  2:4565708/93‑1 (MQ=25)

                               ccTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAAAACAATAACAcc                                                         >  2:1958508/1‑93 (MQ=37)

                                cTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCt                                                        >  1:2817085/1‑93 (MQ=255)

                                 ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                       >  1:6255787/1‑93 (MQ=255)

                                 ttCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                       >  2:5781135/1‑93 (MQ=255)

                                  tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGGACCCCCACGTCCGTAAGAACAGTAACACCTGa                                                      >  1:5803056/1‑93 (MQ=25)

                                  tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa                                                      <  1:4031510/93‑1 (MQ=255)

                                   cGCACAGTCTTCTTTTTTTTTTTGATATCGCTTCTTTGGGG‑GC‑GAG‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGaa                                                     <  1:1203076/93‑1 (MQ=18)

                                   cGCACAGTCATCTTATTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGaa                                                     <  2:4454077/93‑1 (MQ=25)

                                     cacaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGc                                                   >  2:1919608/1‑93 (MQ=255)

                                      acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGCCCGTAAAAACACTAAAACCTGAAGCt                                                  >  2:413668/1‑93 (MQ=25)

                                      acaGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 <  2:5159062/93‑1 (MQ=255)

                                       caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 <  2:3180994/93‑1 (MQ=255)

                                       caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 >  1:3203491/1‑93 (MQ=255)

                                       caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                 >  2:3527407/1‑93 (MQ=255)

                                        aGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAg                                                <  1:817040/93‑1 (MQ=255)

                                         tgtaTCTTACTTTTTTTAATATCGCCTGGTTGGGT‑GC‑GAG‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  1:3304109/90‑1 (MQ=18)

                                         gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  1:1921583/93‑1 (MQ=255)

                                         gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGG‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  2:4264013/93‑1 (MQ=21)

                                         gTCATCTTACTTTTTTCGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgc                                               <  1:273636/93‑1 (MQ=21)

                                          tCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAAACCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                              >  1:2204169/1‑93 (MQ=21)

                                            aTCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                            >  2:5502572/1‑93 (MQ=255)

                                            aTCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGCCCGTAAGAAAACTAACACCTGAAGCTAGcgcg                                            >  2:5275043/1‑93 (MQ=21)

                                             tCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGt                                           >  1:6177298/1‑93 (MQ=255)

                                              cTTACTTTTTTTTATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                         <  1:139000/93‑1 (MQ=255)

                                              cTTACTTTTTTTGATATCGCCTCGTTTGGT‑GCGGG‑‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                          <  1:2609320/93‑1 (MQ=21)

                                               ttACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                         >  1:2284638/1‑93 (MQ=255)

                                               ttACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                         >  2:5951047/1‑93 (MQ=255)

                                               ttACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                        >  1:6213772/1‑93 (MQ=255)

                                               ttACTTTTTTATATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGT‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                         <  1:4198125/93‑1 (MQ=17)

                                                 ttgttttttGATACCGCCTCGTTGGGG‑GG‑GAGGGGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                        <  2:432646/90‑1 (MQ=14)

                                                 aCTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                       >  2:1704337/1‑93 (MQ=255)

                                                   tttttttGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                     >  1:3926931/1‑93 (MQ=255)

                                                     tttttGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                   >  1:473157/1‑93 (MQ=255)

                                                     tttttGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                   >  2:3149510/1‑93 (MQ=255)

                                                     tttttGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                   <  1:3774028/93‑1 (MQ=255)

                                                      ttttGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAAACCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                 >  2:2126853/1‑93 (MQ=255)

                                                        ttGATAGCTCCTCGTGTGGTGGG‑GC‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                <  1:2579740/93‑1 (MQ=14)

                                                         tGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTccc                               >  1:3075200/1‑92 (MQ=17)

                                                         tGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               >  2:1208031/1‑93 (MQ=255)

                                                         tGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               <  1:718356/93‑1 (MQ=255)

                                                         tGATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                               >  2:3589717/1‑93 (MQ=255)

                                                          gATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                              <  2:3682494/93‑1 (MQ=255)

                                                          gATATCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                              >  1:512491/1‑93 (MQ=255)

                                                           atatCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACAGCTGAAGCTAGCGCGTCTACCAATTCCGcc                             >  1:3257421/1‑93 (MQ=17)

                                                           atatCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                             >  2:5462849/1‑93 (MQ=255)

                                                            aagcgccgcGTTTGGT‑GCGGG‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                            <  2:620456/90‑1 (MQ=14)

                                                             tgccccTCTTTGGGT‑GG‑GAG‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                           <  2:3459229/89‑1 (MQ=11)

                                                             atCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAc                           >  1:3659702/1‑93 (MQ=255)

                                                              tCGCCTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCCCGTCTACCAATTCCGCCAcc                          >  2:821100/1‑93 (MQ=14)

                                                               cGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                        >  1:3737849/1‑93 (MQ=255)

                                                               cGCCTCGTTTGGG‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                         <  1:6093550/93‑1 (MQ=14)

                                                                gCCTCGTTTGGG‑GC‑GAGGGGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                         <  2:6283318/93‑1 (MQ=14)

                                                                 gcTCGTTTGGG‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                       <  1:1878041/92‑1 (MQ=14)

                                                                 ccTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                      <  2:5139746/93‑1 (MQ=255)

                                                                 ccTCGTTTGGT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                      >  1:4769310/1‑93 (MQ=255)

                                                                 ccTCGTTTGGC‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCCATTCCGCCACCTTc                       >  1:1074392/1‑93 (MQ=14)

                                                                  cTCGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                      >  1:5163771/1‑93 (MQ=255)

                                                                    cGTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                    >  1:959614/1‑93 (MQ=255)

                                                                     gTTTGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                   >  2:5559125/1‑93 (MQ=255)

                                                                     cttgggT‑GC‑GA‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa                  <  1:5410343/89‑1 (MQ=255)

                                                                      tttGGT‑GC‑GAG‑GGGGGGGGG‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCa                  <  2:4907933/93‑1 (MQ=11)

                                                                      tttGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCGCCCa                  >  2:2627145/1‑93 (MQ=11)

                                                                       ttGGT‑GCGGG‑‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                 <  2:1840166/93‑1 (MQ=11)

                                                                       ttGGT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAg                 <  2:3906980/93‑1 (MQ=255)

                                                                         ggT‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCCCCCCGTg               >  1:4840528/1‑93 (MQ=11)

                                                                           t‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg             >  2:2962243/1‑93 (MQ=255)

                                                                           t‑GC‑GAG‑GGGGGGGGG‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg             <  2:748194/93‑1 (MQ=255)

                                                                                ggagggggggggC‑CTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg           <  2:363390/89‑1 (MQ=14)

                                                                                         gggA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCaa  <  2:3701162/93‑1 (MQ=255)

CCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGT‑GC‑GA‑‑GGGGGGGGA‑CTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAA  >  NC_000913/4603697‑4603874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`