BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,453	T→G	V39G (GTG→GGG)	yjjJ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,453	0	T	G	99.6%	729.6 / NA	228	V39G (GTG→GGG)	yjjJ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (91/136); minor base C (0/1); total (91/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

TGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACG  >  NC_000913/4619363‑4619542

tGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGa                                                                                         <  1:660932/93‑1 (MQ=255)

tGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGa                                                                                         >  2:2347603/1‑93 (MQ=255)

tGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGa                                                                                         >  1:2881772/1‑93 (MQ=255)

 gCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGAt                                                                                        <  1:1133170/93‑1 (MQ=255)

 gCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGAt                                                                                        <  2:1747951/93‑1 (MQ=255)

   aaGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTc                                                                                      <  2:2740573/93‑1 (MQ=255)

   aaGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTc                                                                                      >  1:2307324/1‑93 (MQ=255)

     gggCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGc                                                                                    >  1:4167151/1‑93 (MQ=255)

      ggCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCt                                                                                   <  2:303522/93‑1 (MQ=255)

        ccGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCttt                                                                                 <  2:2562711/93‑1 (MQ=255)

        ccGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCttt                                                                                 <  2:2986393/93‑1 (MQ=255)

           cgTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGt                                                                              <  2:160952/93‑1 (MQ=255)

           cgTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGt                                                                              >  1:1837468/1‑93 (MQ=255)

            gTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTa                                                                             >  1:4185287/1‑93 (MQ=255)

             ttCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTaa                                                                            >  2:6086229/1‑93 (MQ=255)

             ttCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTaa                                                                            >  1:3704750/1‑93 (MQ=255)

              tCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTaaa                                                                           <  2:6256218/93‑1 (MQ=255)

               cTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTTGTAAAg                                                                          >  1:954174/1‑93 (MQ=255)

                tGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGc                                                                         >  2:2675699/1‑93 (MQ=255)

                 gCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTTACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCa                                                                        >  2:2842501/1‑93 (MQ=255)

                 gCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCa                                                                        <  1:1539296/93‑1 (MQ=255)

                 gCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCa                                                                        <  2:3508710/93‑1 (MQ=255)

                 gCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCa                                                                        >  2:3854635/1‑93 (MQ=255)

                  ccccGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCTTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCAc                                                                       <  1:3386953/93‑1 (MQ=255)

                  ccccGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCAc                                                                       >  2:3307721/1‑93 (MQ=255)

                   cccGGAATTGCGCCAGCGGCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACg                                                                      <  1:3152165/93‑1 (MQ=255)

                    ccGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACgg                                                                     >  2:1491086/1‑93 (MQ=255)

                    ccGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACgg                                                                     >  1:4534109/1‑93 (MQ=255)

                    ccGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACgg                                                                     <  1:5998897/93‑1 (MQ=255)

                    ccGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACgg                                                                     <  1:3428175/93‑1 (MQ=255)

                    ccGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACgg                                                                     <  1:1507265/93‑1 (MQ=255)

                     cGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACggg                                                                    >  2:799124/1‑93 (MQ=255)

                     cGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACggg                                                                    <  2:4356392/93‑1 (MQ=255)

                        aaTTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCaa                                                                 <  1:4718468/93‑1 (MQ=255)

                         aTTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAAc                                                                >  1:4346126/1‑93 (MQ=255)

                           tGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAAcgc                                                              <  1:1819952/93‑1 (MQ=255)

                           tGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAAcgc                                                              <  2:3407141/93‑1 (MQ=255)

                               ccAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGata                                                          <  2:5653049/93‑1 (MQ=255)

                                 aGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATg                                                        >  2:2512508/1‑93 (MQ=255)

                                 aGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATg                                                        <  2:5950385/93‑1 (MQ=255)

                                  gCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGc                                                       >  2:858423/1‑93 (MQ=255)

                                    gTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCAc                                                     >  1:455334/1‑93 (MQ=255)

                                     tCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCAct                                                    <  1:6175606/93‑1 (MQ=255)

                                       tGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCActgc                                                  <  2:4661558/93‑1 (MQ=255)

                                        ggCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCActgct                                                 <  1:4023107/93‑1 (MQ=255)

                                          cAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACtgctgc                                               <  1:1323954/93‑1 (MQ=255)

                                            aTCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGt                                             <  2:4582739/93‑1 (MQ=255)

                                             tcagtcagGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTc                                            >  1:5846033/1‑93 (MQ=255)

                                                gtcagGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCtt                                         <  1:4096042/93‑1 (MQ=255)

                                                 tcagGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTa                                        <  1:1276936/93‑1 (MQ=255)

                                                 tcagGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTa                                        <  2:3755247/93‑1 (MQ=255)

                                                  cagGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTAt                                       <  2:222328/93‑1 (MQ=255)

                                                   agGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATc                                      <  1:351955/93‑1 (MQ=255)

                                                   agGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATc                                      <  2:2028462/93‑1 (MQ=255)

                                                     gCGACGTTCTCACGCCTTGTTGCTAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCCCGGGCAACGCGATATGCACTGCTGCGTCCTTATcgc                                    <  2:1237723/93‑1 (MQ=255)

                                                     gCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATcgc                                    <  1:1658789/93‑1 (MQ=255)

                                                     gCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATcgc                                    <  1:1990686/93‑1 (MQ=255)

                                                     gCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATcgc                                    <  1:5657789/93‑1 (MQ=255)

                                                        aCGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGa                                 >  2:6065830/1‑93 (MQ=255)

                                                         cGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGaa                                >  1:586915/1‑93 (MQ=255)

                                                          gTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAAt                               <  1:1958611/93‑1 (MQ=255)

                                                          gTTCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAAt                               >  1:3814921/1‑93 (MQ=255)

                                                           ttCTCACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAAtt                              >  2:5348574/1‑93 (MQ=255)

                                                             ctcACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGa                            >  2:693858/1‑93 (MQ=255)

                                                             ctcACGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGa                            <  2:3981603/93‑1 (MQ=255)

                                                                aCGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCg                         >  2:776119/1‑93 (MQ=255)

                                                                aCGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCg                         >  1:1427181/1‑93 (MQ=255)

                                                                aCGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCg                         <  1:4693136/93‑1 (MQ=255)

                                                                aCGCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCg                         >  1:4966081/1‑93 (MQ=255)

                                                                  gCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTa                       >  1:5212138/1‑93 (MQ=255)

                                                                  gCCTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTa                       <  2:2084146/93‑1 (MQ=255)

                                                                   ccTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTAt                      <  1:4766926/93‑1 (MQ=255)

                                                                   ccTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTAt                      >  1:2081254/1‑93 (MQ=255)

                                                                   ccTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTAt                      <  1:394019/93‑1 (MQ=255)

                                                                    cTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTAtt                     >  2:1943390/1‑93 (MQ=255)

                                                                    cTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTAtt                     >  2:513040/1‑93 (MQ=255)

                                                                    cTTGTTGCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTAtt                     <  2:4762507/93‑1 (MQ=255)

                                                                     ttgttgCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTc                    <  2:5854204/93‑1 (MQ=255)

                                                                     ttgttgCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTc                    <  1:5469689/93‑1 (MQ=255)

                                                                      tgttgCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTcc                   <  2:3333007/93‑1 (MQ=255)

                                                                      tgttgCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTcc                   <  2:5069969/93‑1 (MQ=255)

                                                                      tgttgCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTcc                   <  2:4937350/93‑1 (MQ=255)

                                                                      tgttgCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTcc                   <  1:3599438/93‑1 (MQ=255)

                                                                       gtcgCCAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTccc                  <  1:291122/90‑1 (MQ=255)

                                                                           ccAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCGCGTCt              >  1:6166239/1‑93 (MQ=255)

                                                                           ccAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCt              >  1:6162556/1‑93 (MQ=255)

                                                                            cAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTg             >  2:5554589/1‑93 (MQ=255)

                                                                            cAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTg             >  1:1093799/1‑93 (MQ=255)

                                                                            cAGAGAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTg             >  1:5726042/1‑93 (MQ=255)

                                                                             agagAAGATCGGGGGATTCGCTTTGGTAAAGCACGGTCAACGCAATATGCACTGCTGCGTCCTTATCGCGTAATTGAGCGTATTCCCGTCTgg            <  2:5731552/93‑1 (MQ=255)

                                                                             agagAAGATCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTgg            >  2:1220470/1‑93 (MQ=255)

                                                                                gaagaTCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCgg         <  2:3249098/93‑1 (MQ=255)

                                                                                gaagaTCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCgg         >  2:2753061/1‑93 (MQ=255)

                                                                                gaagaTCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCgg         >  2:1130510/1‑93 (MQ=255)

                                                                                 aagaTCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCggg        <  2:1309543/93‑1 (MQ=255)

                                                                                  agaTCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCgggg       >  1:1046274/1‑92 (MQ=255)

                                                                                  agaTCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGt       <  2:4475104/93‑1 (MQ=255)

                                                                                  agaTCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGt       <  1:5600551/93‑1 (MQ=255)

                                                                                     tCGGGGGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGa    <  1:3058091/93‑1 (MQ=255)

                                                                                       ggggGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACg  <  2:4182897/93‑1 (MQ=255)

TGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACG  >  NC_000913/4619363‑4619542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`