Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 639,910 T→C V322A (GTA→GCA)  ahpF → alkyl hydroperoxide reductase F52a subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913639,9100TC99.6% 788.8 / NA 241V322A (GTA→GCA) ahpFalkyl hydroperoxide reductase F52a subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (102/138);  minor base A (1/0);  total (103/138)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

TTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCG  >  NC_000913/639820‑639999
                                                                                          |                                                                                         
ttGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCAc                                                                                         <  2:1798637/93‑1 (MQ=255)
ttGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCAc                                                                                         <  1:726053/93‑1 (MQ=255)
ttGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCAc                                                                                         <  2:135541/93‑1 (MQ=255)
ttGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCAc                                                                                         <  1:5092995/93‑1 (MQ=255)
 tGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACt                                                                                        <  1:4694661/93‑1 (MQ=255)
   aTGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGa                                                                                      >  2:2136568/1‑93 (MQ=255)
   aTGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGa                                                                                      <  2:1289829/93‑1 (MQ=255)
    tgtgATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGaa                                                                                     <  1:2224036/93‑1 (MQ=255)
     gtgATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGaaa                                                                                    <  2:6198796/93‑1 (MQ=255)
        aTCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCa                                                                                 >  1:5194810/1‑93 (MQ=255)
         tCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCAc                                                                                >  1:5946866/1‑93 (MQ=255)
         tCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCAc                                                                                >  2:349687/1‑93 (MQ=255)
          cGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACg                                                                               >  2:11941/1‑93 (MQ=255)
             cAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACgcag                                                                            >  1:1695651/1‑93 (MQ=255)
                ccAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCAt                                                                         >  1:2512678/1‑93 (MQ=255)
                  agagCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTa                                                                       <  1:3878262/93‑1 (MQ=255)
                  agagCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTa                                                                       <  2:256411/93‑1 (MQ=255)
                  agagCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTa                                                                       <  1:3222169/93‑1 (MQ=255)
                   gagCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTAt                                                                      <  2:4837639/93‑1 (MQ=255)
                     gcgcCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCg                                                                    <  2:1957903/93‑1 (MQ=255)
                     gcgcCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCg                                                                    >  1:3520224/1‑93 (MQ=255)
                      cgcCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGt                                                                   <  2:2947949/93‑1 (MQ=255)
                      cgcCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGt                                                                   <  1:2550458/93‑1 (MQ=255)
                      cgcCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGt                                                                   <  2:5155039/93‑1 (MQ=255)
                       gcCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTg                                                                  <  2:3892665/93‑1 (MQ=255)
                        ccAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTgg                                                                 >  2:3996060/1‑93 (MQ=255)
                          aGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCg                                                               >  2:2760550/1‑93 (MQ=255)
                          aGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCg                                                               <  1:4480120/93‑1 (MQ=255)
                            cAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGAc                                                             >  2:5026713/1‑93 (MQ=255)
                              aaCTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCg                                                           <  1:1563309/93‑1 (MQ=255)
                              aaCTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCg                                                           >  2:2442565/1‑93 (MQ=255)
                                  gATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGAACTGAAAGCACGCAGCATTATCGTGGCGACGGGTGc                                                       >  2:5237653/1‑93 (MQ=255)
                                    tCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCaa                                                     >  2:5080671/1‑93 (MQ=255)
                                     cccAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCaaa                                                    >  2:3461346/1‑93 (MQ=255)
                                      ccAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCaaaa                                                   >  1:4382846/1‑93 (MQ=255)
                                       cagcagcagTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAAt                                                  >  2:3656511/1‑93 (MQ=255)
                                       cagcagcagTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAAt                                                  >  1:5623719/1‑93 (MQ=255)
                                           agcagTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGgcg                                              >  1:4920539/1‑93 (MQ=255)
                                            gcagTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCCCTGCAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGtcgc                                             >  1:1568337/1‑93 (MQ=255)
                                            gcagTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGgcgc                                             >  1:2183975/1‑93 (MQ=255)
                                            gcagTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGgcgc                                             <  1:4186956/93‑1 (MQ=255)
                                              agTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCaa                                           <  2:1070192/93‑1 (MQ=255)
                                              agTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCaa                                           >  2:4361762/1‑93 (MQ=255)
                                               gTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAAc                                          >  2:5586439/1‑93 (MQ=255)
                                                ttGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACa                                         <  2:769304/93‑1 (MQ=255)
                                                   aaGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGa                                      >  1:2962970/1‑93 (MQ=255)
                                                   aaGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGa                                      <  2:3541703/93‑1 (MQ=255)
                                                   aaGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGa                                      <  1:3317799/93‑1 (MQ=255)
                                                   aaGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGa                                      <  2:3188167/93‑1 (MQ=255)
                                                   aaGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGa                                      <  1:4315081/93‑1 (MQ=255)
                                                    aGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGaa                                     >  2:5539721/1‑93 (MQ=255)
                                                    aGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGaa                                     <  2:2277226/93‑1 (MQ=255)
                                                       tggtCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGt                                  >  1:1020524/1‑93 (MQ=255)
                                                        ggtCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGtt                                 <  2:5304082/93‑1 (MQ=255)
                                                        ggtCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGtt                                 <  1:2325920/93‑1 (MQ=255)
                                                        ggtCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGtt                                 <  1:5425412/93‑1 (MQ=255)
                                                        ggtCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGGGCAAAATGGCGCAACATGAACGtt                                 >  2:5378831/1‑93 (MQ=255)
                                                         gtCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTc                                <  2:4673911/93‑1 (MQ=255)
                                                          tCTGCATCAGATTTAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTcc                               <  1:110730/93‑1 (MQ=255)
                                                          tCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTcc                               <  1:4527917/93‑1 (MQ=255)
                                                          tCTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTcc                               <  1:3941940/93‑1 (MQ=255)
                                                           cTGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCg                              >  2:2288776/1‑93 (MQ=255)
                                                            tGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGTTGCAAAATGGCGCAACATGAACGTTCCgg                             <  2:669973/93‑1 (MQ=255)
                                                            tGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCgg                             <  1:4609337/93‑1 (MQ=255)
                                                            tGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCgg                             >  2:441867/1‑93 (MQ=255)
                                                            tGCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCgg                             <  1:5466790/93‑1 (MQ=255)
                                                             gCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCggg                            <  2:1606336/93‑1 (MQ=255)
                                                             gCATCAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCggg                            <  2:2179224/93‑1 (MQ=255)
                                                                 cAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGaa                        >  1:4133010/1‑93 (MQ=255)
                                                                 cAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGaa                        <  2:1865823/93‑1 (MQ=255)
                                                                 cAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGaa                        <  1:2999809/93‑1 (MQ=255)
                                                                 cAGATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGaa                        <  1:767014/93‑1 (MQ=255)
                                                                   gATTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGa                      >  2:6066172/1‑93 (MQ=255)
                                                                    aTTGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGAt                     >  1:6182161/1‑93 (MQ=255)
                                                                     ttGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATc                    >  2:1325357/1‑93 (MQ=255)
                                                                     ttGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATc                    <  1:2435716/93‑1 (MQ=255)
                                                                     ttGAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATc                    <  1:3744406/93‑1 (MQ=255)
                                                                       gAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAg                  >  1:4844138/1‑93 (MQ=255)
                                                                       gAAACAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAg                  <  1:1240432/93‑1 (MQ=255)
                                                                        aaaCAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGt                 <  2:2864533/93‑1 (MQ=255)
                                                                         aaCAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTa                <  1:902676/93‑1 (MQ=255)
                                                                          aCAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATTAACGTTCCGGGCGAAGATCAGTAt               >  2:4938079/1‑93 (MQ=255)
                                                                          aCAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTAt               <  1:3485307/93‑1 (MQ=255)
                                                                          aCAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTAt               >  1:5750990/1‑93 (MQ=255)
                                                                           cAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATc              >  1:5526403/1‑93 (MQ=255)
                                                                           cAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATc              <  1:3764700/93‑1 (MQ=255)
                                                                           cAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATc              <  1:2590476/93‑1 (MQ=255)
                                                                           cAGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATc              >  1:3835462/1‑93 (MQ=255)
                                                                            aGCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCg             >  1:1729068/1‑93 (MQ=255)
                                                                             gCTTCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGc            >  2:5686501/1‑93 (MQ=255)
                                                                               ttCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCAc          >  2:5194466/1‑93 (MQ=255)
                                                                               ttCTGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCAc          >  1:611819/1‑93 (MQ=255)
                                                                                  tGGCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCaa       >  2:5866747/1‑93 (MQ=255)
                                                                                   ggCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCaaa      >  1:2031213/1‑93 (MQ=255)
                                                                                   ggCGCGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCaaa      <  1:3983521/93‑1 (MQ=255)
                                                                                    gcgcGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAg     <  1:1927000/93‑1 (MQ=255)
                                                                                    gcgcGGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAg     <  1:2388245/93‑1 (MQ=255)
                                                                                      gcgGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGc   >  2:4581833/1‑93 (MQ=255)
                                                                                      gcgGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGc   >  1:2782165/1‑93 (MQ=255)
                                                                                      gcgGCACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGc   <  2:3080527/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTGATGTGATCGACAGCCAGAGCGCCAGCAAACTGATCCCAGCAGCAGTTGAAGGTGGTCTGCATCAGATTGAAACAGCTTCTGGCGCGGTACTGAAAGCACGCAGCATTATCGTGGCGACCGGTGCAAAATGGCGCAACATGAACGTTCCGGGCGAAGATCAGTATCGCACCAAAGGCG  >  NC_000913/639820‑639999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑