BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	862,272	T→C	T164A (ACT→GCT)	ybiY ←	putative pyruvate formate‑lyase activating enzyme 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	862,272	0	T	C	100.0%	702.8 / NA	217	T164A (ACT→GCT)	ybiY	putative pyruvate formate‑lyase activating enzyme 2
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (119/98); total (119/98)

Only 100 of 221 total aligned reads displayed.

CGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGC  >  NC_000913/862182‑862361

 gtgtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATg                                                                                        >  2:3620276/1‑93 (MQ=255)

 gtgtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATg                                                                                        >  1:2153927/1‑93 (MQ=255)

   tttttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCGCATGCAGACAGGTTTCTACCGCAGCATGaa                                                                                      <  2:1299442/92‑1 (MQ=255)

   gtTTTAAATCGGCAAGAAACAGGTCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGaa                                                                                      >  1:1600990/1‑93 (MQ=255)

   gtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGaa                                                                                      >  1:3726598/1‑93 (MQ=255)

   gtTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGaa                                                                                      <  2:5624267/93‑1 (MQ=255)

   gtTTTAAAGCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTGCCGCAGCATGaa                                                                                      <  2:2605347/93‑1 (MQ=255)

      ttAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgc                                                                                   <  2:4024844/93‑1 (MQ=255)

          aTCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAAtgcctgc                                                                               >  1:1488568/1‑93 (MQ=255)

            cGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATgcctgcct                                                                             <  1:971983/93‑1 (MQ=255)

               cAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGt                                                                          >  1:440914/1‑93 (MQ=255)

                aaGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtg                                                                         >  1:5383719/1‑93 (MQ=255)

                aaGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtg                                                                         >  2:3658609/1‑93 (MQ=255)

                 aGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtgg                                                                        >  1:4469981/1‑93 (MQ=255)

                 aGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtgg                                                                        >  1:6097680/1‑93 (MQ=255)

                 aGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtgg                                                                        <  1:1986817/93‑1 (MQ=255)

                 aGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtgg                                                                        <  2:3354502/93‑1 (MQ=255)

                   aaaCAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggct                                                                      >  1:3994728/1‑93 (MQ=255)

                      gaGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggctagc                                                                   <  2:5375304/92‑1 (MQ=255)

                      cAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggctggc                                                                   <  2:6074660/93‑1 (MQ=255)

                      cAGATCGATATAGGGCAGAGAAGGCGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGtggcttgc                                                                   <  2:1790057/93‑1 (MQ=255)

                        gATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCtt                                                                 >  1:693179/1‑93 (MQ=255)

                          tCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGc                                                               >  1:469451/1‑93 (MQ=255)

                          tCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGc                                                               <  1:4654682/93‑1 (MQ=255)

                           cGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCGGCCTCGTGGCTGGCTTGCa                                                              <  2:6121024/93‑1 (MQ=255)

                             atatAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGt                                                            >  2:2023823/1‑93 (MQ=255)

                             atatAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGt                                                            <  2:5175235/93‑1 (MQ=255)

                              tataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTa                                                           <  2:115477/93‑1 (MQ=255)

                              tataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTa                                                           >  2:1680761/1‑93 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          <  1:1890304/93‑1 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          >  1:2609746/1‑93 (MQ=255)

                               ataGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAg                                                          <  2:158788/93‑1 (MQ=255)

                                taGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgc                                                         <  1:2946303/93‑1 (MQ=255)

                                 aGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcg                                                        >  1:2473186/1‑93 (MQ=255)

                                  gggCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAgcgc                                                       >  1:6021909/1‑93 (MQ=255)

                                   ggCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgcc                                                      <  2:4908221/93‑1 (MQ=255)

                                    gCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgcca                                                     <  1:4165315/93‑1 (MQ=255)

                                      agagAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatc                                                   <  2:1214598/93‑1 (MQ=255)

                                      agagAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatc                                                   <  2:1137535/93‑1 (MQ=255)

                                      agagAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatc                                                   >  1:2693531/1‑93 (MQ=255)

                                        agaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccctcgc                                                 >  2:1849236/1‑93 (MQ=255)

                                         gaAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgcc                                                >  1:3072847/1‑93 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              >  1:5884044/1‑93 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              <  1:2287948/93‑1 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              >  1:6273866/1‑93 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              >  1:762306/1‑93 (MQ=255)

                                           aGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGcgccatcgccat                                              <  2:3255741/93‑1 (MQ=255)

                                            ggggCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCAtt                                             >  1:3218293/1‑93 (MQ=255)

                                              ggCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTc                                           <  1:5767012/93‑1 (MQ=255)

                                              ggCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTc                                           <  2:1422699/93‑1 (MQ=255)

                                              ggCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTc                                           <  1:2905366/93‑1 (MQ=255)

                                               gCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTcc                                          <  1:5559786/93‑1 (MQ=255)

                                                cGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCg                                         >  1:1122197/1‑93 (MQ=255)

                                                cGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCg                                         >  1:2052342/1‑93 (MQ=255)

                                                 gATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCgg                                        >  1:4045055/1‑93 (MQ=255)

                                                  atatatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCCGc                                       >  1:3756359/1‑93 (MQ=255)

                                                   tatatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTTGCTTGCAGTAGCGCCATCGCCATTTCCGGCt                                      <  2:1771272/93‑1 (MQ=255)

                                                   tatagTTCCACCGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCt                                      <  1:564569/93‑1 (MQ=255)

                                                    atatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTg                                     >  2:307181/1‑93 (MQ=255)

                                                     tatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGc                                    >  1:342185/1‑93 (MQ=255)

                                                     tatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGc                                    <  2:5918212/93‑1 (MQ=255)

                                                     tatTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGc                                    <  1:2032886/93‑1 (MQ=255)

                                                      atTTCCACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCa                                   >  2:5422725/1‑93 (MQ=255)

                                                          ccACGTCATATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaa                               <  2:5834752/93‑1 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaa                               <  2:4089265/93‑1 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaa                               <  2:5270616/93‑1 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaa                               <  1:4538233/93‑1 (MQ=255)

                                                          ccACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATaaa                               <  1:100175/93‑1 (MQ=255)

                                                           cACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAg                              <  2:3497419/93‑1 (MQ=255)

                                                           cACGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAg                              <  2:4409026/93‑1 (MQ=255)

                                                            aCGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCCTAAAgg                             >  1:5031812/1‑93 (MQ=255)

                                                            aCGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAgg                             >  2:2917176/1‑93 (MQ=255)

                                                            aCGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAgg                             <  2:3800628/93‑1 (MQ=255)

                                                             cGGCACATGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAggg                            <  2:1026392/93‑1 (MQ=255)

                                                                cacaTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAggggcc                         >  2:144403/1‑90 (MQ=255)

                                                                  caTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTACGGCTGCATAAAGGGCCcac                       >  2:4805634/1‑93 (MQ=255)

                                                                   aTGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCcacc                      >  2:5483059/1‑93 (MQ=255)

                                                                    tGCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCccacca                     <  1:3838533/93‑1 (MQ=255)

                                                                     gCAGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCcaccac                    >  1:750644/1‑93 (MQ=255)

                                                                       aGACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCAccc                  <  2:499345/93‑1 (MQ=255)

                                                                        gACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCg                 >  1:3528930/1‑93 (MQ=255)

                                                                        gACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCg                 >  2:2622913/1‑93 (MQ=255)

                                                                        gACAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCg                 >  2:5585118/1‑93 (MQ=255)

                                                                          cAGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGaa               >  2:1511866/1‑93 (MQ=255)

                                                                           aGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGaaa              >  1:1278597/1‑93 (MQ=255)

                                                                           aGGTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGaaa              <  2:5705741/93‑1 (MQ=255)

                                                                            ggTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAg             >  2:4285701/1‑93 (MQ=255)

                                                                            ggTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAg             >  2:4687279/1‑93 (MQ=255)

                                                                            ggTTTCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAg             <  1:1843666/93‑1 (MQ=255)

                                                                              tttCTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCg           >  1:5900871/1‑93 (MQ=255)

                                                                                 cTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTa        <  2:2568028/93‑1 (MQ=255)

                                                                                 cTACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTa        >  2:2345440/1‑93 (MQ=255)

                                                                                  tACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTaa       >  2:4027942/1‑93 (MQ=255)

                                                                                  tACCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTaa       >  2:4442556/1‑93 (MQ=255)

                                                                                   aCCGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTaaa      <  2:2750998/93‑1 (MQ=255)

                                                                                    ccGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAAc     >  1:3234573/1‑93 (MQ=255)

                                                                                    ccGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAAc     <  2:6283697/93‑1 (MQ=255)

                                                                                     cGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAAcc    >  1:5154777/1‑93 (MQ=255)

                                                                                     cGCAGCATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGAGTTAAAcc    >  2:2159211/1‑93 (MQ=255)

                                                                                      gcagcaTGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCCCACCACCCGAAAGCGTTAAAccg   <  1:3345598/93‑1 (MQ=255)

CGTGTTTTAAATCGGCAAGAAACAGATCGATATAGGGCAGAGAAGGGGCGATATATTTCCACGGCACATGCAGACAGGTTTCTACCGCAGTATGAATGCCTGCCTCGTGGCTGGCTTGCAGTAGCGCCATCGCCATTTCCGGCTGCATAAAGGGCTCACCACCCGAAAGCGTTAAACCGC  >  NC_000913/862182‑862361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`