BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	79,855	A→G	V337A (GTG→GCG)	leuC ←	3‑isopropylmalate isomerase (dehydratase) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	79,855	0	A	G	100.0%	742.3 / NA	233	V337A (GTG→GCG)	leuC	3‑isopropylmalate isomerase (dehydratase) subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (126/107); total (126/107)

Only 100 of 242 total aligned reads displayed.

ACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCC  >  NC_000913/79765‑79944

aCTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCt                                                                                         >  1:3973105/1‑93 (MQ=255)

 cTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCTAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCtt                                                                                        >  1:624449/1‑93 (MQ=255)

 cTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCtt                                                                                        >  1:1424145/1‑93 (MQ=255)

 cTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCtt                                                                                        <  1:520320/93‑1 (MQ=255)

 cTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCtt                                                                                        <  1:3576220/93‑1 (MQ=255)

  ttttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTc                                                                                       >  2:293745/1‑93 (MQ=255)

  ttttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTc                                                                                       <  1:1633099/93‑1 (MQ=255)

   tttCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCg                                                                                      <  2:3898328/93‑1 (MQ=255)

     tCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGt                                                                                    >  1:5669732/1‑93 (MQ=255)

     tCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGt                                                                                    <  2:209436/93‑1 (MQ=255)

         ccTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGc                                                                                <  2:3383000/93‑1 (MQ=255)

         ccTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGc                                                                                <  1:3889361/93‑1 (MQ=255)

          cTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCg                                                                               <  1:2217726/93‑1 (MQ=255)

          cTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCg                                                                               >  1:2448773/1‑93 (MQ=255)

          cTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCg                                                                               >  1:200680/1‑93 (MQ=255)

          cTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCg                                                                               >  2:190988/1‑93 (MQ=255)

           tttGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCgg                                                                              >  2:2586152/1‑93 (MQ=255)

             tGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGaa                                                                            <  1:4406160/93‑1 (MQ=255)

              ggCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAAt                                                                           <  1:3040152/93‑1 (MQ=255)

                cGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAc                                                                         <  2:1151414/93‑1 (MQ=255)

                 gATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAcc                                                                        <  1:4098782/93‑1 (MQ=255)

                  aTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAccc                                                                       >  1:2030745/1‑93 (MQ=255)

                  aTCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATAccc                                                                       >  2:5961603/1‑93 (MQ=255)

                    ctcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCgg                                                                     >  1:3432754/1‑93 (MQ=255)

                     tcCGCTGCCGCGCGTAAATCTTCCATGCGCGAGTTGGGACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCATCGGAATACGCGGt                                                                    >  2:2406384/1‑93 (MQ=255)

                     tcCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGt                                                                    <  1:5416593/93‑1 (MQ=255)

                       cGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGttt                                                                  >  1:154355/1‑93 (MQ=255)

                        gCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTc                                                                 <  1:5244325/93‑1 (MQ=255)

                        gCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTc                                                                 >  1:3304890/1‑93 (MQ=255)

                         cTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCa                                                                >  2:5910497/1‑93 (MQ=255)

                         cTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCa                                                                <  1:3138994/93‑1 (MQ=255)

                         cTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCa                                                                <  1:1092580/93‑1 (MQ=255)

                           gCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGGACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGc                                                              >  2:1182655/1‑93 (MQ=255)

                              gcgcgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGcccc                                                           >  2:52410/1‑93 (MQ=255)

                              gcgcgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGcccc                                                           <  1:4419556/93‑1 (MQ=255)

                               cgcgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCa                                                          <  1:3247708/93‑1 (MQ=255)

                                gcgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCat                                                         >  2:2159000/1‑93 (MQ=255)

                                gcgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCat                                                         >  1:4561723/1‑93 (MQ=255)

                                 cgTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCata                                                        <  2:5042760/93‑1 (MQ=255)

                                  gTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCatat                                                       >  1:2247523/1‑93 (MQ=255)

                                  gTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCatat                                                       >  2:3400348/1‑93 (MQ=255)

                                   tAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCAtata                                                      >  1:4353453/1‑93 (MQ=255)

                                    aaaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAg                                                     <  2:4675009/93‑1 (MQ=255)

                                     aaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGTTTTCAGCCCCATATAgg                                                    >  2:6041246/1‑93 (MQ=255)

                                     aaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAgg                                                    >  2:3299195/1‑93 (MQ=255)

                                     aaTCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAgg                                                    <  1:6112941/93‑1 (MQ=255)

                                       tCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGcc                                                  >  1:5959629/1‑93 (MQ=255)

                                          tCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAgc                                               >  2:5717853/1‑93 (MQ=255)

                                          tCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAgc                                               >  1:3784954/1‑93 (MQ=255)

                                             aTGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCt                                            <  1:4772820/93‑1 (MQ=255)

                                              tGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCtt                                           <  1:2792515/93‑1 (MQ=255)

                                                cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCtttt                                         <  2:5444652/93‑1 (MQ=255)

                                                cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCtttt                                         <  2:385668/93‑1 (MQ=255)

                                                cgcgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCtttt                                         <  2:4102888/93‑1 (MQ=255)

                                                  cgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTc                                       <  1:6120831/93‑1 (MQ=255)

                                                  cgAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTc                                       <  2:1509668/93‑1 (MQ=255)

                                                   gAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCt                                      >  2:6172734/1‑93 (MQ=255)

                                                   gAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCt                                      >  2:3769360/1‑93 (MQ=255)

                                                   gAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCt                                      >  1:1718666/1‑93 (MQ=255)

                                                     gTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGc                                    <  2:493419/93‑1 (MQ=255)

                                                     gTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGc                                    >  2:4479771/1‑93 (MQ=255)

                                                     gTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGc                                    <  1:2663501/93‑1 (MQ=255)

                                                      ttGGTACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGcc                                   <  2:1751359/93‑1 (MQ=255)

                                                          tACAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcg                               >  1:1440121/1‑93 (MQ=255)

                                                           aCAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgc                              <  1:2523448/93‑1 (MQ=255)

                                                           aCAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgc                              <  2:4707225/93‑1 (MQ=255)

                                                           aCAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgc                              <  1:2896725/93‑1 (MQ=255)

                                                           aCAGGAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGAcgc                              <  1:6097690/93‑1 (MQ=255)

                                                               gAACCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGt                          >  1:5214100/1‑93 (MQ=255)

                                                                aaCCGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGtt                         <  1:82823/93‑1 (MQ=255)

                                                                  ccGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCa                       <  2:136247/93‑1 (MQ=255)

                                                                  ccGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCa                       >  1:5773700/1‑93 (MQ=255)

                                                                  ccGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCa                       <  2:4390245/93‑1 (MQ=255)

                                                                   cGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCaa                      >  2:1432000/1‑93 (MQ=255)

                                                                   cGATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCaa                      >  1:5389384/1‑93 (MQ=255)

                                                                    gATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAc                     <  2:3457622/93‑1 (MQ=255)

                                                                    gATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAc                     <  1:4604218/93‑1 (MQ=255)

                                                                    gATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAc                     >  1:2488769/1‑93 (MQ=255)

                                                                    gATAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAc                     >  2:4096548/1‑93 (MQ=255)

                                                                     aTAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAcc                    <  2:3153554/93‑1 (MQ=255)

                                                                     aTAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAcc                    >  1:508727/1‑93 (MQ=255)

                                                                     aTAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAAcc                    <  1:4461518/93‑1 (MQ=255)

                                                                      tAAACACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCg                   >  2:780916/1‑93 (MQ=255)

                                                                        aaCACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGa                 <  1:1152332/93‑1 (MQ=255)

                                                                        aaCACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGa                 <  1:5881521/93‑1 (MQ=255)

                                                                        aaCACTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGa                 >  1:359553/1‑93 (MQ=255)

                                                                         acacTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGAt                <  2:5849915/93‑1 (MQ=255)

                                                                           acTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCg              >  1:6161283/1‑93 (MQ=255)

                                                                            cTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCgg             <  2:4258236/93‑1 (MQ=255)

                                                                            cTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCgg             <  2:2323145/93‑1 (MQ=255)

                                                                            cTTTGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCgg             >  1:4801841/1‑93 (MQ=255)

                                                                             tttGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGc            <  1:4697224/93‑1 (MQ=255)

                                                                               tGTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCaa          <  2:311998/93‑1 (MQ=255)

                                                                                gTCGATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCaaa         >  2:3332317/1‑93 (MQ=255)

                                                                                 tCGATATCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATTTAGGCATGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAAc        <  2:811931/93‑1 (MQ=255)

                                                                                   gATAGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGa      >  2:3529158/1‑93 (MQ=255)

                                                                                      aGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGc   <  1:1300895/93‑1 (MQ=255)

                                                                                      aGCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCCACGCGCGTTCAACCCGCTCGGCAAACGAAGc   >  2:2782810/1‑93 (MQ=255)

                                                                                       gCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGcc  <  1:4961761/93‑1 (MQ=255)

                                                                                       gCCGCTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGcc  >  1:3017314/1‑93 (MQ=255)

ACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTGTCGATAGCCACTTCGGTCAGCGGAATACCCGGTTTCAGCCCCATATAGGCCAGCGCTTTTTCTGCCGACGCGCGTTCAACCGGATCGGCAAACGAAGCC  >  NC_000913/79765‑79944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`