Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 905,051 T→C E321G (GAG→GGG)  b0868 ← putative nucleotide di‑P‑sugar epimerase or dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913905,0510TC99.5% 611.9 / NA 190E321G (GAG→GGG) b0868putative nucleotide di‑P‑sugar epimerase or dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (87/102);  minor base G (1/0);  total (88/102)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

GATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTG  >  NC_000913/904961‑905140
                                                                                          |                                                                                         
   tAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTcc                                                                                      <  2:1786189/93‑1 (MQ=255)
   tAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTcc                                                                                      <  1:3774804/93‑1 (MQ=255)
    aGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACTGGCTGATAACCTAACGCTTCCt                                                                                     >  2:149316/1‑93 (MQ=255)
    aGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCt                                                                                     <  2:3013790/93‑1 (MQ=255)
     gcgcGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTg                                                                                    <  1:3263300/93‑1 (MQ=255)
      cgcgGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTgc                                                                                   >  2:5566246/1‑93 (MQ=255)
       gcgGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTgcg                                                                                  >  2:4269753/1‑93 (MQ=255)
       gcgGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTgcg                                                                                  >  1:5221117/1‑93 (MQ=255)
         ggCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCa                                                                                <  1:5634311/93‑1 (MQ=255)
         ggCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCa                                                                                >  1:2608067/1‑93 (MQ=255)
            aGTTTTCCGTGGTCGCGTAGCCAGGTGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCAcgc                                                                             >  1:3116765/1‑93 (MQ=255)
             gTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCAcgcg                                                                            <  2:955641/93‑1 (MQ=255)
              ttttCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGt                                                                           >  2:2156890/1‑93 (MQ=255)
               tttCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTa                                                                          >  1:1129206/1‑93 (MQ=255)
                ttCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTaa                                                                         <  1:2885292/93‑1 (MQ=255)
                ttCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTaa                                                                         <  2:2013652/93‑1 (MQ=255)
                  ccGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAata                                                                       >  2:6302455/1‑93 (MQ=255)
                  ccGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAata                                                                       >  1:1686172/1‑93 (MQ=255)
                  ccGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAata                                                                       <  1:166291/93‑1 (MQ=255)
                  ccGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAata                                                                       <  2:5884359/93‑1 (MQ=255)
                    gTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATc                                                                     <  2:615480/93‑1 (MQ=255)
                      ggTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCa                                                                   >  1:5467582/1‑93 (MQ=255)
                       gTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAg                                                                  <  1:4393608/93‑1 (MQ=255)
                       gTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAg                                                                  <  2:5666517/93‑1 (MQ=255)
                            gTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTaa                                                             >  2:4373521/1‑93 (MQ=255)
                             tAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTaaa                                                            >  2:5182863/1‑93 (MQ=255)
                             tAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTaaa                                                            <  2:6123966/93‑1 (MQ=255)
                              aGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAg                                                           <  2:3821858/93‑1 (MQ=255)
                                  aGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaa                                                       >  1:814640/1‑93 (MQ=255)
                                  aGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaa                                                       >  2:5750783/1‑93 (MQ=255)
                                   ggcggcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaaa                                                      >  1:5986014/1‑93 (MQ=255)
                                    gcggcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaaaa                                                     <  1:5177225/93‑1 (MQ=255)
                                      ggcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAAtt                                                   <  1:4937396/93‑1 (MQ=255)
                                       gcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTc                                                  >  1:5811381/1‑93 (MQ=255)
                                       gcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTc                                                  >  2:2754041/1‑93 (MQ=255)
                                       gcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTc                                                  <  2:4995172/93‑1 (MQ=255)
                                       gcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTc                                                  >  1:4261848/1‑93 (MQ=255)
                                       gcAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTc                                                  >  1:4151750/1‑93 (MQ=255)
                                         aGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCTAGCGTAAAGTCAAAATTCaa                                                >  2:3361717/1‑93 (MQ=255)
                                          gTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAAc                                               >  2:5267840/1‑93 (MQ=255)
                                          gTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAAc                                               <  2:488209/93‑1 (MQ=255)
                                          gTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAAc                                               <  1:3467281/93‑1 (MQ=255)
                                           ttttCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACt                                              >  1:1806682/1‑93 (MQ=255)
                                             ttCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTg                                            <  1:1753555/93‑1 (MQ=255)
                                             ttCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCATTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTg                                            >  2:4117522/1‑93 (MQ=255)
                                              tctcGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTgg                                           <  2:5456099/93‑1 (MQ=255)
                                               ctcGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGga                                          <  1:3508939/93‑1 (MQ=255)
                                                tcGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGgag                                         >  1:3688416/1‑93 (MQ=255)
                                                 cGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGgaga                                        <  2:4779371/93‑1 (MQ=255)
                                                  gATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGAc                                       <  2:2840838/93‑1 (MQ=255)
                                                   aTACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACg                                      >  1:4152526/1‑93 (MQ=255)
                                                   aTACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACg                                      >  2:3337876/1‑93 (MQ=255)
                                                   aTACCTTCAACCAGGGTGATGACCGGCTGGTAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACg                                      >  2:3168275/1‑93 (MQ=255)
                                                    tACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGc                                     <  1:2962826/93‑1 (MQ=255)
                                                    tACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGc                                     >  1:2834862/1‑93 (MQ=255)
                                                      ccTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCg                                   <  1:4282552/93‑1 (MQ=255)
                                                       cTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGt                                  <  2:3216712/93‑1 (MQ=255)
                                                       cTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGt                                  >  2:2512248/1‑93 (MQ=255)
                                                       cTTCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGt                                  >  2:4735523/1‑93 (MQ=255)
                                                        ttCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTa                                 >  2:3260888/1‑93 (MQ=255)
                                                        ttCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTa                                 >  1:1043919/1‑93 (MQ=255)
                                                         tCATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAg                                <  2:268970/93‑1 (MQ=255)
                                                          cATCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGt                               >  2:4563087/1‑93 (MQ=255)
                                                           aTCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTg                              >  1:1562487/1‑93 (MQ=255)
                                                           aTCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTg                              >  1:4542453/1‑93 (MQ=255)
                                                            tCCAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTgg                             >  2:819309/1‑93 (MQ=255)
                                                              cAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGt                           >  2:876290/1‑93 (MQ=255)
                                                              cAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGt                           >  1:163224/1‑93 (MQ=255)
                                                              cAGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGt                           <  2:1043169/93‑1 (MQ=255)
                                                               aGGGTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTc                          <  2:1116770/93‑1 (MQ=255)
                                                                gggTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCa                         >  1:2237276/1‑93 (MQ=255)
                                                                gggTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCa                         >  2:1506061/1‑93 (MQ=255)
                                                                gggTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCa                         >  2:3845944/1‑93 (MQ=255)
                                                                 ggTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAg                        >  2:2603686/1‑93 (MQ=255)
                                                                  gTGATGACCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgc                       <  1:3036685/93‑1 (MQ=255)
                                                                   tgatgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcg                      <  1:1919448/93‑1 (MQ=255)
                                                                    gatgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcgg                     <  1:2154762/93‑1 (MQ=255)
                                                                    gatgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcgg                     >  1:4040370/1‑93 (MQ=255)
                                                                    gatgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcgg                     >  2:5244910/1‑93 (MQ=255)
                                                                      tgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcggcg                   <  1:2864468/93‑1 (MQ=255)
                                                                      tgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcggcg                   >  2:212901/1‑93 (MQ=255)
                                                                      tgaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAgcggcg                   >  2:4206164/1‑93 (MQ=255)
                                                                       gaCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGcggcgg                  >  1:3915018/1‑93 (MQ=255)
                                                                        aCCGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCggcggc                 <  2:1743801/93‑1 (MQ=255)
                                                                         ccGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGct                <  1:6035296/93‑1 (MQ=255)
                                                                          cGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTTGGTCAGCGGCGGctc               >  1:5112914/1‑93 (MQ=255)
                                                                          cGGCTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGctc               >  1:2032529/1‑93 (MQ=255)
                                                                             cTGATAACCTATCCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCttt            <  2:3563422/93‑1 (MQ=255)
                                                                             cTGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCttt            <  1:913017/93‑1 (MQ=255)
                                                                              tGATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCtttt           <  2:3970153/93‑1 (MQ=255)
                                                                               gATAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTg          >  1:5399899/1‑93 (MQ=255)
                                                                                aTAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGc         <  2:2553396/93‑1 (MQ=255)
                                                                                aTAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGc         <  1:1397549/93‑1 (MQ=255)
                                                                                aTAACCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGc         <  2:5958639/93‑1 (MQ=255)
                                                                                  aaCCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTg       >  1:4825175/1‑93 (MQ=255)
                                                                                   aCCTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGa      >  2:3791297/1‑93 (MQ=255)
                                                                                     cTAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACt    >  2:2473866/1‑93 (MQ=255)
                                                                                      tAACCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACtt   >  1:3979605/1‑93 (MQ=255)
                                                                                       aaCCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACttc  >  1:656054/1‑92 (MQ=255)
                                                                                       aaCCCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTg  >  2:5304264/1‑93 (MQ=255)
                                                                                          |                                                                                         
GATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGACGCCGTAGTGGGTCAGCGGCGGCTCTTTTGCTGACTTG  >  NC_000913/904961‑905140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑