BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	974,364	A→G	I275V (ATT→GTT)	mukF →	MukF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	974,364	0	A	G	99.5%	670.0 / NA	210	I275V (ATT→GTT)	mukF	MukF
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (114/94); minor base C (0/1); total (114/96)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTC  >  NC_000913/974274‑974452

gATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGtt                                                                                        >  2:4589166/1‑93 (MQ=255)

 aTCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTg                                                                                       >  1:5043453/1‑93 (MQ=255)

 aTCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTg                                                                                       >  2:2573469/1‑93 (MQ=255)

  tCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTgg                                                                                      >  1:4452571/1‑93 (MQ=255)

  tCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTgg                                                                                      >  1:1866519/1‑93 (MQ=255)

  tCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTgg                                                                                      <  2:3766921/93‑1 (MQ=255)

   cTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGc                                                                                     <  1:4169779/93‑1 (MQ=255)

    tGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCt                                                                                    >  1:819592/1‑93 (MQ=255)

    tGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCt                                                                                    >  1:3400012/1‑93 (MQ=255)

       aTTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGGTGGCTACg                                                                                 >  2:1791170/1‑93 (MQ=255)

        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCACTCCATCGACTTGTGGGTTGGCTACGa                                                                                <  1:5456772/93‑1 (MQ=255)

        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACgc                                                                                >  1:3867380/1‑92 (MQ=255)

        tttCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGa                                                                                <  1:5837302/93‑1 (MQ=255)

          tcgtcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGAcc                                                                              <  1:3260489/93‑1 (MQ=255)

            gtcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGc                                                                            >  2:5486974/1‑93 (MQ=255)

            gtcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGc                                                                            >  1:3493194/1‑93 (MQ=255)

             tcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGcc                                                                           >  2:172391/1‑93 (MQ=255)

             tcgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGcc                                                                           >  2:1378594/1‑93 (MQ=255)

              cgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCa                                                                          <  2:1023354/93‑1 (MQ=255)

              cgATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCa                                                                          >  1:3580819/1‑93 (MQ=255)

               gATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCAc                                                                         >  1:5971732/1‑93 (MQ=255)

                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACg                                                                        >  2:4297206/1‑93 (MQ=255)

                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACg                                                                        >  2:1208199/1‑93 (MQ=255)

                aTCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACg                                                                        >  1:1609279/1‑93 (MQ=255)

                  cGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTa                                                                      <  1:209766/93‑1 (MQ=255)

                  cGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTa                                                                      <  1:3111764/93‑1 (MQ=255)

                   gTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTac                                                                     >  2:5145220/1‑93 (MQ=255)

                    tCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTaca                                                                    >  1:3426018/1‑93 (MQ=255)

                      tGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTAcaca                                                                  >  2:5337328/1‑93 (MQ=255)

                          gtTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAtt                                                              <  2:2749040/93‑1 (MQ=255)

                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                             >  2:6001082/1‑93 (MQ=255)

                           ttCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAAttt                                                             >  2:498121/1‑93 (MQ=255)

                            tCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTa                                                            >  1:2846156/1‑93 (MQ=255)

                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                         <  2:597072/93‑1 (MQ=255)

                               aTCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTc                                                         <  1:4974057/93‑1 (MQ=255)

                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                        <  1:1667054/93‑1 (MQ=255)

                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                        >  1:2951998/1‑93 (MQ=255)

                                tCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCg                                                        >  2:2960889/1‑93 (MQ=255)

                                 cTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGt                                                       >  1:3176431/1‑93 (MQ=255)

                                 cTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGt                                                       <  2:3693922/93‑1 (MQ=255)

                                  tggagagCAAGCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTGGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGtg                                                      <  1:1924131/90‑2 (MQ=255)

                                  tGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTa                                                      <  1:4127249/93‑1 (MQ=255)

                                   gCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTAc                                                     >  2:2099070/1‑93 (MQ=255)

                                     agagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcg                                                   <  2:2459323/93‑1 (MQ=255)

                                     agagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcg                                                   >  2:6217674/1‑93 (MQ=255)

                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                  <  1:3819451/93‑1 (MQ=255)

                                      gagCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTCGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACcgc                                                  <  1:3384558/93‑1 (MQ=255)

                                         cAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgat                                               >  1:4731146/1‑93 (MQ=255)

                                         cAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgat                                               <  1:3922901/93‑1 (MQ=255)

                                          aaaCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatc                                              >  1:1230617/1‑93 (MQ=255)

                                          aaaCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatc                                              <  2:5526582/93‑1 (MQ=255)

                                            aCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcga                                            >  1:5769130/1‑93 (MQ=255)

                                            aCTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcga                                            >  2:4263340/1‑93 (MQ=255)

                                             cTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGcgatcgat                                           >  1:1374797/1‑93 (MQ=255)

                                              tCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGata                                          <  1:1191553/93‑1 (MQ=255)

                                                 aTCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATgg                                       >  2:5375972/1‑93 (MQ=255)

                                                  tCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGa                                      >  1:1004643/1‑93 (MQ=255)

                                                   cGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAt                                     >  1:5383670/1‑93 (MQ=255)

                                                   cGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAt                                     >  2:4212128/1‑93 (MQ=255)

                                                   cGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGAt                                     <  2:287134/93‑1 (MQ=255)

                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                  >  1:5340511/1‑93 (MQ=255)

                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                  <  1:1308715/93‑1 (MQ=255)

                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                  >  2:5022914/1‑93 (MQ=255)

                                                      attatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaa                                  >  2:2388656/1‑93 (MQ=255)

                                                       ttatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaa                                 >  1:4043998/1‑93 (MQ=255)

                                                        tatCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATaaaaa                                <  2:5126263/93‑1 (MQ=255)

                                                          tCAGTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAAcc                              >  1:4280582/1‑93 (MQ=255)

                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgcg                           >  2:1556065/1‑93 (MQ=255)

                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgcg                           <  1:6104249/93‑1 (MQ=255)

                                                             gTTGGGGCCAGCAATCCATCGACTTGTGGCTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACcgcg                           <  2:2280448/93‑1 (MQ=255)

                                                               tGGGGCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTc                         >  1:4544672/1‑93 (MQ=255)

                                                                ggggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCt                        <  1:2298168/93‑1 (MQ=255)

                                                                 gggCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCtt                       <  2:1258687/93‑1 (MQ=255)

                                                                   gCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTg                     <  2:2696539/93‑1 (MQ=255)

                                                                   gCCAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTg                     <  1:3069679/93‑1 (MQ=255)

                                                                    ccAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCTTCTTTGc                    <  1:5493554/93‑1 (MQ=255)

                                                                    ccAGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGc                    <  1:3229787/93‑1 (MQ=255)

                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  <  1:4080739/93‑1 (MQ=255)

                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  >  1:5273951/1‑93 (MQ=255)

                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  <  2:5225583/93‑1 (MQ=255)

                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  >  2:4408335/1‑93 (MQ=255)

                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  >  1:6213543/1‑93 (MQ=255)

                                                                      aGCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTc                  >  2:4084058/1‑93 (MQ=255)

                                                                       ttaaTCCATCGACTTGTGGGATGGCTACTACCGCCACGTACTCAAATTTATTTGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTcg                 >  2:6113703/3‑92 (MQ=255)

                                                                       gCAATCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCa                 <  1:2475271/93‑1 (MQ=255)

                                                                         aaTCCATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGc               >  1:148490/1‑93 (MQ=255)

                                                                            ccATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGt            >  2:19371/1‑93 (MQ=255)

                                                                             cATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGtt           >  1:2175017/1‑93 (MQ=255)

                                                                             cATCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGtt           >  2:1647810/1‑93 (MQ=255)

                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa          >  2:3945156/1‑93 (MQ=255)

                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa          <  2:581186/93‑1 (MQ=255)

                                                                              aTCGACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTa          <  1:5939862/93‑1 (MQ=255)

                                                                                 gACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGt       <  2:3653222/93‑1 (MQ=255)

                                                                                 gACTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGt       <  1:2749411/93‑1 (MQ=255)

                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc      <  1:727616/93‑1 (MQ=255)

                                                                                  aCTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTc      <  1:2922893/93‑1 (MQ=255)

                                                                                   cTTGTGGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCa     >  2:3060504/1‑93 (MQ=255)

                                                                                     tgtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGt   <  1:5151751/93‑1 (MQ=255)

                                                                                      gtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTc  <  1:2022692/93‑1 (MQ=255)

                                                                                      gtgGGTTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTc  <  1:4641722/93‑1 (MQ=255)

GATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTCGATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCACGTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGGTTACGTCAGTC  >  NC_000913/974274‑974452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`