BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,815	C→T	S61L (TCG→TTG)	yabB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,815	0	C	T	99.6%	907.9 / NA	240	S61L (TCG→TTG)	yabB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (139/100); minor base G (0/1); total (139/101)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

CCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTC  >  NC_000913/89725‑89904

ccGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATtgc                                                                                         <  2:4208218/93‑1 (MQ=255)

ccGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATtgc                                                                                         <  2:4721479/93‑1 (MQ=255)

 cGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATtgcg                                                                                        >  2:467214/1‑93 (MQ=255)

 cGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATtgcg                                                                                        >  2:5381935/1‑93 (MQ=255)

  ggTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGt                                                                                       >  1:6186908/1‑93 (MQ=255)

   gTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCTCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTc                                                                                      >  1:3000740/1‑93 (MQ=255)

   gTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTc                                                                                      <  1:1074043/93‑1 (MQ=255)

   gTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTc                                                                                      <  2:1137652/93‑1 (MQ=255)

   gTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTc                                                                                      <  2:1777940/93‑1 (MQ=255)

   gTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTc                                                                                      <  2:3177353/93‑1 (MQ=255)

   gTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTc                                                                                      >  2:3625247/1‑93 (MQ=255)

   gTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTc                                                                                      <  1:1215093/93‑1 (MQ=255)

    tCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCt                                                                                     <  1:476813/93‑1 (MQ=255)

    tCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCt                                                                                     <  1:4537574/93‑1 (MQ=255)

     cAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTg                                                                                    >  1:6045893/1‑93 (MQ=255)

       aaTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTc                                                                                  <  2:137941/93‑1 (MQ=255)

        aTGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCg                                                                                 <  2:1318552/93‑1 (MQ=255)

           gTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGc                                                                              >  1:1318917/1‑93 (MQ=255)

            tttGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCa                                                                             >  2:2516358/1‑93 (MQ=255)

              tGCACCAGTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATg                                                                           <  1:2259796/93‑1 (MQ=255)

                cACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGaa                                                                         >  1:5830358/1‑93 (MQ=255)

                 aCCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAAc                                                                        >  2:3832071/1‑93 (MQ=255)

                 aCCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAAc                                                                        <  1:2053606/93‑1 (MQ=255)

                  ccATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAAcc                                                                       <  2:6279383/93‑1 (MQ=255)

                  ccATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAAcc                                                                       >  1:3896235/1‑93 (MQ=255)

                    aTTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCg                                                                     <  2:5047041/93‑1 (MQ=255)

                     ttGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCgg                                                                    >  1:2820418/1‑93 (MQ=255)

                      tGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGt                                                                   <  2:6225687/93‑1 (MQ=255)

                      tGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGt                                                                   <  2:2729006/93‑1 (MQ=255)

                      tGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGt                                                                   >  1:5110302/1‑93 (MQ=255)

                         cATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGa                                                                <  1:3479503/93‑1 (MQ=255)

                          aTTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAg                                                               >  1:3553534/1‑93 (MQ=255)

                           tttATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAgc                                                              <  1:4799362/93‑1 (MQ=255)

                           tttATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAgc                                                              >  1:1004800/1‑93 (MQ=255)

                             tATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAgcgc                                                            >  2:5393748/1‑93 (MQ=255)

                              aTCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGcc                                                           <  2:193361/93‑1 (MQ=255)

                                cACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCgt                                                         >  2:2636379/1‑93 (MQ=255)

                                cACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATCGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGGATGAACCCGGTTGAGCGCCgg                                                         >  2:3345317/1‑92 (MQ=255)

                                 aCCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCgtg                                                        >  1:196355/1‑93 (MQ=255)

                                 aCCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCgtg                                                        >  2:3055475/1‑93 (MQ=255)

                                     gTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCa                                                    >  2:33759/1‑93 (MQ=255)

                                      tGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAg                                                   >  2:2580324/1‑93 (MQ=255)

                                       gCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAgc                                                  >  2:5820047/1‑93 (MQ=255)

                                        ccTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAgcg                                                 >  1:1567484/1‑93 (MQ=255)

                                          tgctgcTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGcc                                               <  1:2475808/93‑1 (MQ=255)

                                          tgctgcTTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGcc                                               <  2:1131319/93‑1 (MQ=255)

                                           gctgctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCt                                              <  2:3523392/93‑1 (MQ=255)

                                            ctgctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCtc                                             >  1:2566023/1‑92 (MQ=255)

                                             tgctTTACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTAc                                            <  2:4392280/93‑1 (MQ=255)

                                                tttACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGt                                         <  2:2096588/93‑1 (MQ=255)

                                                  tACCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTa                                       >  1:1765689/1‑93 (MQ=255)

                                                   aCCCCCTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAg                                      <  1:2892428/93‑1 (MQ=255)

                                                    cccccTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAgg                                     >  2:984262/1‑93 (MQ=255)

                                                    cccccTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAgg                                     <  2:1070025/93‑1 (MQ=255)

                                                     ccccTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGt                                    >  1:1696358/1‑93 (MQ=255)

                                                     ccccTGCCTGAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGt                                    <  1:2668886/93‑1 (MQ=255)

                                                       cctgcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCa                                  >  1:4627273/1‑93 (MQ=255)

                                                        ctgcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCAt                                 <  2:554959/93‑1 (MQ=255)

                                                         tgcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATg                                <  2:1465024/93‑1 (MQ=255)

                                                          gcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGc                               >  2:728870/1‑93 (MQ=255)

                                                          gcctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGc                               >  1:5124850/1‑93 (MQ=255)

                                                           cctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGcc                              <  1:1234048/93‑1 (MQ=255)

                                                            ctgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCa                             <  1:6259775/93‑1 (MQ=255)

                                                             tgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAg                            >  1:3466091/1‑93 (MQ=255)

                                                             tgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAg                            >  2:502575/1‑93 (MQ=255)

                                                             tgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAg                            <  1:2426037/93‑1 (MQ=255)

                                                             tgAATGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAg                            >  2:2197834/1‑93 (MQ=255)

                                                               aaTGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCg                          >  1:5994346/1‑93 (MQ=255)

                                                               aaTGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCg                          >  2:3273237/1‑93 (MQ=255)

                                                                 tGGGAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGaa                        >  2:4459503/1‑93 (MQ=255)

                                                                  gggAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAAt                       >  2:5517795/1‑93 (MQ=255)

                                                                  gggAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAAt                       <  1:3579740/93‑1 (MQ=255)

                                                                   ggAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATg                      >  1:1994857/1‑93 (MQ=255)

                                                                   ggAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATg                      <  1:5231958/93‑1 (MQ=255)

                                                                    gAAATTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGt                     >  1:3738595/1‑93 (MQ=255)

                                                                     aaaTTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTc                    >  1:2498716/1‑93 (MQ=255)

                                                                       aTTATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAg                  <  2:567496/93‑1 (MQ=255)

                                                                        ttATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAga                 <  1:5029315/93‑1 (MQ=255)

                                                                        ttATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAga                 <  2:5219956/93‑1 (MQ=255)

                                                                        ttATCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAga                 <  1:1655374/93‑1 (MQ=255)

                                                                          aTCAAGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatg               >  1:5717697/1‑93 (MQ=255)

                                                                           tcaaGCAAAAATTATTGCGTCTGTCGAGCATGAGCCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatgg              <  1:4123461/90‑1 (MQ=255)

                                                                            caaGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatgga             >  2:3942264/3‑93 (MQ=255)

                                                                            caaGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatgga             >  1:1389903/3‑93 (MQ=255)

                                                                            caaGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatgga             >  2:2963062/3‑93 (MQ=255)

                                                                             aaGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatggat            >  2:3839308/2‑93 (MQ=255)

                                                                             aaGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatggat            >  2:1518319/2‑93 (MQ=255)

                                                                              aGCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAgatggatg           <  2:2021829/93‑1 (MQ=255)

                                                                               gCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGatggatgg          >  2:6156175/1‑93 (MQ=255)

                                                                               gCAAAAATTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGatggatgg          >  1:662159/1‑93 (MQ=255)

                                                                                 aaaaaTTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGgcg        >  1:2345813/1‑93 (MQ=255)

                                                                                   aaaTTATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCa      <  1:4377213/93‑1 (MQ=255)

                                                                                     attattGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAgg    <  1:718248/93‑1 (MQ=255)

                                                                                     attattGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAgg    <  1:5194058/93‑1 (MQ=255)

                                                                                     attattGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAgg    <  1:4956913/93‑1 (MQ=255)

                                                                                      ttATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGt   >  1:1870896/1‑93 (MQ=255)

                                                                                      ttATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGt   <  1:3271732/93‑1 (MQ=255)

                                                                                      ttATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGt   >  2:3770842/1‑93 (MQ=255)

                                                                                       tATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTc  <  1:1526686/93‑1 (MQ=255)

                                                                                       tATTGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTc  >  1:4497583/1‑93 (MQ=255)

CCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAGCAAAAATTATCGCGTCTGTCGAGCATGAACCCGGTTGAGCGCCGTGTGCAGCGCCTACTGTTAGGTCATGCCAGCGAATGTCAGATGGATGGCGCAGGTC  >  NC_000913/89725‑89904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`