BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	988,555	A→G	L342P (CTG→CCG)	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	988,555	0	A	G	100.0%	578.5 / NA	181	L342P (CTG→CCG)	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (92/89); total (92/89)

Only 100 of 190 total aligned reads displayed.

CTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAG  >  NC_000913/988465‑988644

gtGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCggg                                                                                         <  2:3069473/92‑1 (MQ=255)

cTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCggg                                                                                         <  1:2956559/93‑1 (MQ=255)

 tGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGc                                                                                        >  2:5941416/1‑93 (MQ=255)

  gTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCg                                                                                       <  1:1606773/93‑1 (MQ=255)

   tCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGa                                                                                      <  1:2327835/93‑1 (MQ=255)

    cAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAg                                                                                     <  1:3783723/93‑1 (MQ=255)

    cAGAAAGTTTCGCCACCGGATTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAg                                                                                     >  2:1721826/1‑93 (MQ=255)

     aGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGt                                                                                    >  2:66579/1‑93 (MQ=255)

     aGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGt                                                                                    >  1:314655/1‑93 (MQ=255)

     aGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGt                                                                                    >  1:342316/1‑93 (MQ=255)

        aaGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTccc                                                                                 <  2:2712927/93‑1 (MQ=255)

          gTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCaa                                                                               <  2:5674213/93‑1 (MQ=255)

          gTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCaa                                                                               >  2:1825755/1‑93 (MQ=255)

           tttCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAAt                                                                              <  2:2348228/93‑1 (MQ=255)

           tttCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAAt                                                                              <  2:1183105/93‑1 (MQ=255)

           tttCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAAt                                                                              <  1:1456895/93‑1 (MQ=255)

             tCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATAc                                                                            >  2:5565224/1‑93 (MQ=255)

             tCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATAc                                                                            >  2:48227/1‑93 (MQ=255)

              cGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATAcc                                                                           <  2:5530167/93‑1 (MQ=255)

                ccACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaa                                                                         >  1:5778166/1‑93 (MQ=255)

                  aCCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaaa                                                                       <  1:881443/93‑1 (MQ=255)

                  aCCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaaa                                                                       >  2:5243143/1‑93 (MQ=255)

                  aCCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaaa                                                                       <  1:5210877/93‑1 (MQ=255)

                  aCCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaaa                                                                       >  2:3873352/1‑93 (MQ=255)

                  aCCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCaaaa                                                                       >  2:4082019/1‑93 (MQ=255)

                         tACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAAtt                                                                <  1:2118742/93‑1 (MQ=255)

                            cGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCa                                                             <  2:1408443/93‑1 (MQ=255)

                            cGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCa                                                             <  2:3523736/93‑1 (MQ=255)

                             gTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAc                                                            >  2:1442344/1‑93 (MQ=255)

                              ttACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcg                                                           <  1:1768180/93‑1 (MQ=255)

                              ttACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcg                                                           >  2:3578131/1‑93 (MQ=255)

                                acacTCTACCAACTTAATGACAATATTCAGGTGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCAcgcg                                                         <  2:2040240/93‑1 (MQ=255)

                                 cacTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCgg                                                        <  2:5755160/93‑1 (MQ=255)

                                   ctctACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGa                                                      <  1:2703742/93‑1 (MQ=255)

                                    tctACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGTGGGaa                                                     <  2:4785979/93‑1 (MQ=255)

                                    tctACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGaa                                                     <  1:5322979/93‑1 (MQ=255)

                                      tACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAaga                                                   <  2:5578413/93‑1 (MQ=255)

                                      tACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAaga                                                   >  1:6003245/1‑93 (MQ=255)

                                       aCCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAagag                                                  <  1:1683888/93‑1 (MQ=255)

                                        ccAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAgaga                                                 <  1:3886228/93‑1 (MQ=255)

                                        ccAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAgaga                                                 >  1:4596691/1‑93 (MQ=255)

                                         cAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGaa                                                <  2:3817766/93‑1 (MQ=255)

                                          aaCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAg                                               <  1:1927502/93‑1 (MQ=255)

                                           aattAATGACAATATTCAGGGGGTTTACCTGGGGGATATCGCAGGTCGGGCGAGGCCCAATACCAAAAACTAATTGCACGCGGGAAgaggagg                                              >  2:5880567/3‑92 (MQ=255)

                                           aCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAgg                                              >  2:4915842/1‑92 (MQ=255)

                                           aCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAga                                              >  2:2789071/1‑92 (MQ=255)

                                           aCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGt                                              >  1:3244556/1‑93 (MQ=255)

                                           aCTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGt                                              >  2:3986387/1‑93 (MQ=255)

                                            cTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTg                                             >  1:339403/1‑93 (MQ=255)

                                             ttAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTgg                                            <  1:426682/93‑1 (MQ=255)

                                             ttAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTgg                                            <  2:3560722/93‑1 (MQ=255)

                                               aaTGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCg                                          >  1:2170936/1‑93 (MQ=255)

                                                aTGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCgg                                         <  2:3316659/93‑1 (MQ=255)

                                                aTGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCgg                                         >  2:5048282/1‑93 (MQ=255)

                                                  gACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGta                                       <  1:1878779/93‑1 (MQ=255)

                                                  gACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGta                                       <  2:6178313/93‑1 (MQ=255)

                                                  gACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGta                                       >  2:102996/1‑93 (MQ=255)

                                                   aCAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGtat                                      <  2:4874275/93‑1 (MQ=255)

                                                    cAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGtata                                     <  2:1496411/93‑1 (MQ=255)

                                                      atatTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGc                                   >  1:833433/1‑93 (MQ=255)

                                                        atTCAGGGGTTTTACCTGGGTGATAGCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCATGCGGGAAGAGAAGTGGCGGTATAGCTc                                 <  1:1537720/93‑1 (MQ=255)

                                                        atTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTc                                 <  1:6161410/93‑1 (MQ=255)

                                                        atTCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTc                                 <  2:4165193/93‑1 (MQ=255)

                                                         ttCAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCa                                >  1:5009585/1‑93 (MQ=255)

                                                           cAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAc                              >  1:3500641/1‑93 (MQ=255)

                                                           cAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAc                              <  1:3642401/93‑1 (MQ=255)

                                                           cAGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAc                              >  2:253693/1‑93 (MQ=255)

                                                           cAGGGGTGTTACCTGGGTGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAc                              <  1:3117583/93‑1 (MQ=255)

                                                            aGGGGTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAAcc                             >  2:2130995/1‑93 (MQ=255)

                                                             ggggTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCg                            >  1:420655/1‑93 (MQ=255)

                                                             ggggTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCg                            >  2:1572651/1‑93 (MQ=255)

                                                                gTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCtt                         <  1:5955520/93‑1 (MQ=255)

                                                                gTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCtt                         >  1:2013235/1‑93 (MQ=255)

                                                                gTTTTACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCtt                         >  2:5181144/1‑93 (MQ=255)

                                                                 ttttACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCttt                        <  1:3190752/93‑1 (MQ=255)

                                                                 ttttACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCttt                        <  2:5417583/93‑1 (MQ=255)

                                                                 ttttACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCttt                        <  2:196849/93‑1 (MQ=255)

                                                                    tACCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCg                     >  2:5575135/1‑93 (MQ=255)

                                                                     aCCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGt                    >  1:2059275/1‑93 (MQ=255)

                                                                     aCCTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGt                    >  1:3947313/1‑93 (MQ=255)

                                                                       cTGGGGGATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTaa                  >  1:2464656/1‑93 (MQ=255)

                                                                          ggggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATTGCTCAACCGCTTTGCGTAAATc               >  1:4755526/1‑93 (MQ=255)

                                                                          ggggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATc               >  1:1172850/1‑93 (MQ=255)

                                                                           gggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATcc              >  2:758103/1‑93 (MQ=255)

                                                                            ggATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCa             <  2:2946239/93‑1 (MQ=255)

                                                                             gATATCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAg            <  1:2880523/93‑1 (MQ=255)

                                                                              atatCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGa           <  2:4627685/93‑1 (MQ=255)

                                                                              atatCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGa           >  2:5208088/1‑93 (MQ=255)

                                                                              atatCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGa           <  1:985592/93‑1 (MQ=255)

                                                                               tatCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGAt          >  1:2930062/1‑93 (MQ=255)

                                                                                 tCGCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTg        <  2:3640119/93‑1 (MQ=255)

                                                                                   gCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATGCAGAATGTc      >  1:6056062/1‑93 (MQ=255)

                                                                                   gCAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTc      <  2:242381/93‑1 (MQ=255)

                                                                                    cAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCa     <  1:5483890/93‑1 (MQ=255)

                                                                                    cAGGTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCa     <  1:4891616/93‑1 (MQ=255)

                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTTTCaga   >  2:30989/1‑93 (MQ=255)

                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   <  2:1267141/93‑1 (MQ=255)

                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   <  1:5539518/93‑1 (MQ=255)

                                                                                      ggTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCaga   >  1:108977/1‑93 (MQ=255)

                                                                                       gTCGGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCagag  >  2:4245053/1‑93 (MQ=255)

CTGTCAGAAAGTTTCGCCACCGGTTTACCGTTACACTCTACCAACTTAATGACAATATTCAGGGGTTTTACCTGGGGGATATCGCAGGTCAGGCGAGTCCCAATACCAAAACTTAATTGCACGCGGGAAGAGAAGTGGCGGTATAGCTCAACCGCTTTGCGTAAATCCAGATTGTCAGAG  >  NC_000913/988465‑988644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`