BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,035,719	C→T	T48M (ACG→ATG)	hyaE →	HyaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,035,719	0	C	T	99.6%	859.0 / NA	231	T48M (ACG→ATG)	hyaE	HyaE
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (110/120); total (110/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

GCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTG  >  NC_000913/1035629‑1035808

gCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAacgcatgc                                                                                         >  1:4922609/1‑93 (MQ=255)

   ggACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCag                                                                                      >  1:5475959/1‑93 (MQ=255)

   ggACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCag                                                                                      <  1:2115025/93‑1 (MQ=255)

    gACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCaga                                                                                     <  1:4043336/93‑1 (MQ=255)

    gACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCaga                                                                                     <  2:1009662/93‑1 (MQ=255)

    gACGCCAGTCAGTGAACCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCaga                                                                                     <  2:2440744/93‑1 (MQ=255)

       gCCAGTCAGTTAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGt                                                                                  <  2:641009/93‑1 (MQ=255)

        ccAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTc                                                                                 <  2:322925/93‑1 (MQ=255)

        ccAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTc                                                                                 <  1:5064182/93‑1 (MQ=255)

         cagtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCa                                                                                >  2:82334/1‑93 (MQ=255)

         cagtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCa                                                                                <  2:534057/93‑1 (MQ=255)

          agtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAg                                                                               >  1:1954220/1‑93 (MQ=255)

           gtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGc                                                                              <  1:4791391/93‑1 (MQ=255)

           gtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGc                                                                              <  1:3623677/93‑1 (MQ=255)

           gtcagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGCGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGc                                                                              >  1:1514902/1‑93 (MQ=255)

            ccagtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGGGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCg                                                                             >  2:4067801/2‑93 (MQ=255)

              agtGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGAt                                                                           <  1:919196/93‑1 (MQ=255)

                tGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATaa                                                                         >  1:1293627/1‑93 (MQ=255)

                  aaTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATc                                                                       <  2:5410243/93‑1 (MQ=255)

                  aaTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGGCAGCGATAATc                                                                       >  1:1387011/1‑93 (MQ=255)

                   aTCCCGTCTTGACGACTGTCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATcc                                                                      <  2:5546031/93‑1 (MQ=255)

                   aTCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATcc                                                                      <  2:1309621/93‑1 (MQ=255)

                    tCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCg                                                                     >  2:1369987/1‑93 (MQ=255)

                    acccGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCg                                                                     <  1:2021680/92‑1 (MQ=255)

                     cccGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCgg                                                                    >  1:1725779/1‑93 (MQ=255)

                     cccGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAAGTCAGCGATAATCCgg                                                                    >  2:1983906/1‑93 (MQ=255)

                      ccGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGt                                                                   >  2:4536374/1‑93 (MQ=255)

                       cGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTa                                                                  >  2:1590776/1‑93 (MQ=255)

                       cGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTa                                                                  >  2:1795136/1‑93 (MQ=255)

                        gTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTaa                                                                 >  1:3651347/1‑93 (MQ=255)

                        gTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTaa                                                                 >  1:76349/1‑93 (MQ=255)

                         tCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAAt                                                                >  1:1310662/1‑93 (MQ=255)

                         tCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAAt                                                                >  1:1089588/1‑93 (MQ=255)

                             gacgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGAtt                                                            >  1:6203646/1‑93 (MQ=255)

                              acgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTg                                                           <  2:5833546/93‑1 (MQ=255)

                              acgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTg                                                           <  1:6104048/93‑1 (MQ=255)

                               cgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTgg                                                          <  2:5249200/93‑1 (MQ=255)

                               cgacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTgg                                                          <  1:247256/93‑1 (MQ=255)

                                gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGc                                                         >  2:2223189/1‑93 (MQ=255)

                                gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGc                                                         >  1:3531901/1‑93 (MQ=255)

                                gacTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGc                                                         <  2:390555/93‑1 (MQ=255)

                                 acTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCg                                                        <  1:386133/93‑1 (MQ=255)

                                  cTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGa                                                       <  2:4157038/93‑1 (MQ=255)

                                  cTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGa                                                       <  1:2917672/93‑1 (MQ=255)

                                  cTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGa                                                       >  1:2555558/1‑93 (MQ=255)

                                   tGGCTTCCGCAGGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGaa                                                      <  2:5444349/93‑1 (MQ=255)

                                   tGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGaa                                                      >  2:3696364/1‑93 (MQ=255)

                                    ggCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAt                                                     >  1:867789/1‑93 (MQ=255)

                                     gCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAAtt                                                    >  2:3308092/1‑93 (MQ=255)

                                      cTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTa                                                   <  1:6042714/93‑1 (MQ=255)

                                      cTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTa                                                   <  1:2686066/93‑1 (MQ=255)

                                      cTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTa                                                   <  2:2842871/93‑1 (MQ=255)

                                       ttACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTAc                                                  >  2:4852919/1‑93 (MQ=255)

                                          cGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgc                                               <  1:2165357/93‑1 (MQ=255)

                                           gCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgcg                                              >  1:112054/1‑93 (MQ=255)

                                           gCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTgcg                                              >  1:1176053/1‑93 (MQ=255)

                                              aGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGa                                           <  2:3450283/93‑1 (MQ=255)

                                                 gcCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGtt                                        <  2:2623741/93‑1 (MQ=255)

                                                    aGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTcc                                     <  1:3195839/93‑1 (MQ=255)

                                                    aGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTcc                                     >  2:2765436/1‑93 (MQ=255)

                                                     gACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTccc                                    <  2:3186509/93‑1 (MQ=255)

                                                       cGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGa                                  <  2:4942065/93‑1 (MQ=255)

                                                        ggCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc                                 >  1:2955906/1‑93 (MQ=255)

                                                        ggCGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGAc                                 >  1:4500115/1‑93 (MQ=255)

                                                          cGTGGTGTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACta                               >  1:1837775/1‑93 (MQ=255)

                                                           gtggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtat                              >  2:1508164/1‑93 (MQ=255)

                                                           gtggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtat                              >  1:3694753/1‑93 (MQ=255)

                                                           gtggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtat                              >  1:4077328/1‑93 (MQ=255)

                                                           gtggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACtat                              >  2:6215345/1‑93 (MQ=255)

                                                             ggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAc                            >  1:5545847/1‑93 (MQ=255)

                                                             ggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAc                            >  2:1485333/1‑93 (MQ=255)

                                                             ggtgTTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATAc                            >  2:5885739/1‑93 (MQ=255)

                                                              gtgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACa                           <  1:4807235/93‑1 (MQ=255)

                                                               tgtTATTAAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACAt                          >  1:5805215/1‑93 (MQ=255)

                                                                 ttattaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATgg                        <  1:5124961/93‑1 (MQ=255)

                                                                 ttattaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATgg                        <  2:4499844/93‑1 (MQ=255)

                                                                  tattaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGc                       <  2:256388/93‑1 (MQ=255)

                                                                   attaAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCa                      >  1:2845484/1‑93 (MQ=255)

                                                                     taAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAgg                    >  2:5105073/1‑93 (MQ=255)

                                                                     taAGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAgg                    >  1:4354671/1‑93 (MQ=255)

                                                                      aaGCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGt                   <  1:5212068/93‑1 (MQ=255)

                                                                        gCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTgg                 >  1:4517342/1‑93 (MQ=255)

                                                                        gCAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTgg                 >  2:2193826/1‑93 (MQ=255)

                                                                         cAGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGc                <  2:1848111/93‑1 (MQ=255)

                                                                          aGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCg               >  1:1776204/1‑93 (MQ=255)

                                                                          aGTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGGGGCg               >  2:5627243/1‑93 (MQ=255)

                                                                           gTGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGa              >  2:3826663/1‑93 (MQ=255)

                                                                            tGACCCGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGAt             <  2:4292419/93‑1 (MQ=255)

                                                                               cccGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGc          <  1:23700/93‑1 (MQ=255)

                                                                                ccGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCt         >  2:5299552/1‑93 (MQ=255)

                                                                                 cGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTg        >  1:5901913/1‑93 (MQ=255)

                                                                                 cGAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTg        >  1:3056001/1‑93 (MQ=255)

                                                                                  gAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGa       >  2:1169342/1‑93 (MQ=255)

                                                                                  gAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGa       >  2:3897687/1‑93 (MQ=255)

                                                                                  gAAACGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGa       >  1:4233961/1‑93 (MQ=255)

                                                                                   aaaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAc      <  2:5148945/93‑1 (MQ=255)

                                                                                   aaaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAc      >  1:715222/1‑93 (MQ=255)

                                                                                    aaCGCATGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGAcc     >  2:176639/1‑93 (MQ=255)

                                                                                      cgcatgcCAGAGGTCAGCTATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCtt   <  1:2037618/93‑1 (MQ=255)

                                                                                       gcatgcCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTg  >  2:4721297/1‑93 (MQ=255)

GCTGGACGCCAGTCAGTGAATCCCGTCTTGACGACTGGCTTACGCAAGCGCCAGACGGCGTGGTGTTATTAAGCAGTGACCCGAAACGCACGCCAGAGGTCAGCGATAATCCGGTAATGATTGGCGAATTACTGCGCGAGTTTCCCGACTATACATGGCAGGTGGCGATTGCTGACCTTG  >  NC_000913/1035629‑1035808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`