BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	112,238	A→G	V121A (GTG→GCG)	yacF ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	112,238	0	A	G	99.5%	707.1 / NA	219	V121A (GTG→GCG)	yacF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (116/102); minor base C (0/1); total (116/103)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

CGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATT  >  NC_000913/112149‑112327

  cgcTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCaga                                                                                      >  2:4836526/1‑93 (MQ=255)

    cTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGc                                                                                    >  1:4894586/1‑93 (MQ=255)

      gcgcCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCaa                                                                                  >  2:4634217/1‑93 (MQ=255)

        gcCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATc                                                                                <  1:4249307/93‑1 (MQ=255)

        gcCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATc                                                                                <  2:3307429/93‑1 (MQ=255)

           tGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCaaa                                                                             >  2:1939751/1‑93 (MQ=255)

           tGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCaaa                                                                             <  1:532334/93‑1 (MQ=255)

           tGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCaaa                                                                             >  2:3849753/1‑93 (MQ=255)

           tGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCaaa                                                                             <  1:789824/93‑1 (MQ=255)

             gggTGGATGCAGCCAAGTGTACAATGTAGGTAAACCAAAGCTGCAACAGCCGCGGGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACg                                                                           <  2:337451/93‑1 (MQ=255)

             gggTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACg                                                                           >  1:4078092/1‑93 (MQ=255)

              ggTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGa                                                                          >  1:3996981/1‑93 (MQ=255)

               gTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGAt                                                                         >  2:1688574/1‑93 (MQ=255)

                tAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATc                                                                        >  1:5723950/1‑93 (MQ=255)

                 aGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCt                                                                       <  1:2458352/93‑1 (MQ=255)

                  gATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCtt                                                                      <  2:5721119/93‑1 (MQ=255)

                   aTGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTc                                                                     >  1:409598/1‑93 (MQ=255)

                    tGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCa                                                                    <  2:670306/93‑1 (MQ=255)

                     gCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCAc                                                                   <  2:605338/93‑1 (MQ=255)

                     gCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCAc                                                                   <  1:3215784/93‑1 (MQ=255)

                     gCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCAc                                                                   <  1:5562250/93‑1 (MQ=255)

                     gCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCAc                                                                   <  2:1442621/93‑1 (MQ=255)

                     gCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCAc                                                                   <  1:2181576/93‑1 (MQ=255)

                      cAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACg                                                                  >  1:1355598/1‑93 (MQ=255)

                       aGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGc                                                                 >  1:5696149/1‑93 (MQ=255)

                        gCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCa                                                                >  2:307425/1‑93 (MQ=255)

                        gCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCa                                                                >  1:4750629/1‑93 (MQ=255)

                        gCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCa                                                                <  1:1245523/93‑1 (MQ=255)

                        gCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCa                                                                <  1:745625/93‑1 (MQ=255)

                         ccAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAg                                                               >  1:376725/1‑93 (MQ=255)

                         ccAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAg                                                               >  1:2641171/1‑93 (MQ=255)

                          cAAATGTGCAATGTAGGTAAATCAAAGCTGCACCAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGa                                                              <  2:4397649/93‑1 (MQ=255)

                          cAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGa                                                              >  2:2285098/1‑93 (MQ=255)

                          cAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGa                                                              >  1:5185266/1‑93 (MQ=255)

                           aaaTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATTCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGaa                                                             >  1:5823390/1‑93 (MQ=255)

                           aaaTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGaa                                                             >  1:1949620/1‑93 (MQ=255)

                             aTGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAt                                                           <  2:2033493/93‑1 (MQ=255)

                              tgtgCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAtt                                                          <  2:107329/93‑1 (MQ=255)

                              tgtgCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAtt                                                          <  2:4608142/93‑1 (MQ=255)

                              tgtgCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAAtt                                                          <  1:2132573/93‑1 (MQ=255)

                                 gCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGcc                                                       <  2:5108635/93‑1 (MQ=255)

                                 gCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGcc                                                       >  1:5277055/1‑93 (MQ=255)

                                  cAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGccc                                                      >  2:5599482/1‑93 (MQ=255)

                                   aaTGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAGCGCTCTTCACGCAGAAATTGCCCg                                                     <  2:4011787/93‑1 (MQ=255)

                                   aaTGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCg                                                     >  2:4124783/1‑93 (MQ=255)

                                   aaTGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCg                                                     >  2:5175885/1‑93 (MQ=255)

                                    aTGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGa                                                    <  1:2594839/93‑1 (MQ=255)

                                     tGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGAt                                                   >  2:3432058/1‑93 (MQ=255)

                                     tGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGAt                                                   >  2:1102217/1‑93 (MQ=255)

                                      gTAGTTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCCCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATa                                                  <  2:3207708/93‑1 (MQ=255)

                                      gTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATa                                                  >  1:802975/1‑93 (MQ=255)

                                      gTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATa                                                  >  2:4532593/1‑93 (MQ=255)

                                       tAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAc                                                 <  2:4569352/93‑1 (MQ=255)

                                        aGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcg                                                <  1:2878138/93‑1 (MQ=255)

                                        aGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcg                                                >  2:3575764/1‑93 (MQ=255)

                                         ggTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgc                                               >  2:2953347/1‑93 (MQ=255)

                                         ggTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgc                                               >  1:1184865/1‑93 (MQ=255)

                                         ggTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgc                                               >  2:6136804/1‑93 (MQ=255)

                                         ggTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgc                                               >  2:5842374/1‑93 (MQ=255)

                                          gTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcg                                              >  1:2931908/1‑93 (MQ=255)

                                           tAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcgg                                             <  1:4350611/93‑1 (MQ=255)

                                            aaaTCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggc                                            <  1:1343171/93‑1 (MQ=255)

                                                cAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcgcgg                                        >  2:5956583/1‑93 (MQ=255)

                                                  aaGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGaa                                      >  2:3026604/1‑93 (MQ=255)

                                                   aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGaaa                                     >  1:3621520/1‑93 (MQ=255)

                                                   aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGaaa                                     >  1:5913445/1‑93 (MQ=255)

                                                    gCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAAt                                    <  2:1981798/93‑1 (MQ=255)

                                                       gCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTaa                                 <  2:3282130/93‑1 (MQ=255)

                                                       gCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTaa                                 >  1:5409865/1‑93 (MQ=255)

                                                       gCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTaa                                 >  2:2375763/1‑93 (MQ=255)

                                                         aaCAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATa                               <  2:5254457/93‑1 (MQ=255)

                                                          aCAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATAc                              <  2:26031/93‑1 (MQ=255)

                                                          aCAGCCGCCTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATAc                              <  2:2464416/93‑1 (MQ=255)

                                                             gccgccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCt                           <  2:3562272/93‑1 (MQ=255)

                                                             gccgccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCt                           >  2:831220/1‑93 (MQ=255)

                                                             gccgccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCt                           <  2:1718120/93‑1 (MQ=255)

                                                                gccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTccc                        >  2:4320858/1‑93 (MQ=255)

                                                                gccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTccc                        >  1:2753440/1‑93 (MQ=255)

                                                                gccTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTccc                        >  2:728862/1‑93 (MQ=255)

                                                                  cTGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccg                      <  1:2306304/93‑1 (MQ=255)

                                                                   tGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATGCGCGGCGCGGAAATTAATACGCTCCccgc                     <  1:6123898/93‑1 (MQ=255)

                                                                   tGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgc                     >  1:4309793/1‑93 (MQ=255)

                                                                   tGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgc                     >  1:4880413/1‑93 (MQ=255)

                                                                   tGGGATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgc                     >  2:2174236/1‑93 (MQ=255)

                                                                    gggATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgcc                    >  2:1519184/1‑93 (MQ=255)

                                                                    gggATGCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgcc                    >  1:4662483/1‑93 (MQ=255)

                                                                         gCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCttt               >  2:2807945/1‑93 (MQ=255)

                                                                         gCTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCttt               >  2:5987128/1‑93 (MQ=255)

                                                                          cTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCtttt              >  1:3532358/1‑93 (MQ=255)

                                                                          cTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCtttt              >  1:1593736/1‑93 (MQ=255)

                                                                          cTCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCtttt              <  2:90963/93‑1 (MQ=255)

                                                                           tCAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTa             >  1:1844785/1‑93 (MQ=255)

                                                                            cAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTaa            <  2:577027/93‑1 (MQ=255)

                                                                            cAGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTaa            <  2:5929250/93‑1 (MQ=255)

                                                                             aGTCGCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAAc           <  1:3902351/93‑1 (MQ=255)

                                                                                 gCTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAActgct       <  1:6034729/93‑1 (MQ=255)

                                                                                  cTGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAActgctg      <  2:3537315/93‑1 (MQ=255)

                                                                                   tGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGa     <  2:5448695/93‑1 (MQ=255)

                                                                                   tGACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGa     <  1:1046959/93‑1 (MQ=255)

                                                                                    gACGCGCCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGaa    <  2:1234428/93‑1 (MQ=255)

CGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATT  >  NC_000913/112149‑112327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`