BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,137,414	A→G	D274G (GAC→GGC)	flgJ →	FlgJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,137,414	0	A	G	99.5%	669.4 / ‑4.8	214	D274G (GAC→GGC)	flgJ	FlgJ
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (92/121); total (93/121)
Fisher's exact test for biased strand distribution p-value = 4.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

Only 100 of 221 total aligned reads displayed.

CGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAA  >  NC_000913/1137324‑1137503

cGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGcg                                                                                         <  1:4480623/93‑1 (MQ=255)

 ggATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGgcgc                                                                                        <  1:1076681/93‑1 (MQ=255)

  gATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGcgcg                                                                                       >  2:5569972/1‑93 (MQ=255)

  gATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGcgcg                                                                                       >  2:3140380/1‑93 (MQ=255)

   aTTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCgg                                                                                      <  2:1805443/93‑1 (MQ=255)

   aTTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCgg                                                                                      <  1:6307048/93‑1 (MQ=255)

     tACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGc                                                                                    <  1:1005636/93‑1 (MQ=255)

         ttGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATg                                                                                >  2:1911761/1‑93 (MQ=255)

         ttGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATg                                                                                >  2:2188248/1‑93 (MQ=255)

          tGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGc                                                                               <  2:6151047/93‑1 (MQ=255)

          tGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGc                                                                               >  2:4431342/1‑93 (MQ=255)

          tGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGc                                                                               >  2:2354262/1‑93 (MQ=255)

           gggCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGcc                                                                              <  1:3931384/93‑1 (MQ=255)

            ggCTGTTAACGCGTACCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCa                                                                             <  2:6210710/93‑1 (MQ=255)

            ggCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCa                                                                             >  2:4215402/1‑93 (MQ=255)

            ggCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCa                                                                             >  1:3679381/1‑93 (MQ=255)

             gCTGTTAACGCGTACCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCAc                                                                            <  1:1204635/93‑1 (MQ=255)

              cTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCAcc                                                                           <  1:2953800/93‑1 (MQ=255)

              cTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCAcc                                                                           >  2:5515857/1‑93 (MQ=255)

              cTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGACCTACAGGGCGCGGGCTATGACAcc                                                                           >  2:1476062/1‑93 (MQ=255)

               tGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCg                                                                          >  2:5617421/1‑93 (MQ=255)

                  tAACGCGTACCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATc                                                                       <  2:3263012/93‑1 (MQ=255)

                    actcgTACCCCGCGCTACGCCGCCGTGACGTCCGCCGCGAGTGCGGAACAGGGGGCCCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCt                                                                     <  2:814678/90‑1 (MQ=255)

                      gcgTACCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCa                                                                   <  1:4306923/93‑1 (MQ=255)

                       cgTACCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCAc                                                                  <  1:1546683/93‑1 (MQ=255)

                        gTACCCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACt                                                                 <  2:259263/93‑1 (MQ=255)

                        gTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGGCCTACAGGGCGCGGGCTATGGCACCGATCCTCACt                                                                 >  2:3355908/1‑93 (MQ=255)

                        gTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACt                                                                 >  2:1222851/1‑93 (MQ=255)

                         taccccGCGCTCCGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGACCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTa                                                                <  2:2664526/90‑1 (MQ=255)

                             ccGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGcc                                                            >  2:1400001/1‑93 (MQ=255)

                              cgcgCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGccc                                                           >  1:2829451/1‑93 (MQ=255)

                                   tACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCaaa                                                      <  2:5711978/93‑1 (MQ=255)

                                     cgccgcCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACt                                                    <  1:5512985/93‑1 (MQ=255)

                                      gccgccGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTc                                                   <  1:471159/93‑1 (MQ=255)

                                           cgTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCaa                                              >  2:4833638/1‑93 (MQ=255)

                                            gTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAAc                                             >  1:1225508/1‑93 (MQ=255)

                                             tGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACa                                            <  1:5265940/93‑1 (MQ=255)

                                             tGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACa                                            <  1:2729073/93‑1 (MQ=255)

                                               acgacCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATg                                          <  1:3460260/93‑1 (MQ=255)

                                                cgacCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGa                                         >  1:2488084/1‑93 (MQ=255)

                                                 gacCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGAt                                        <  2:6107068/93‑1 (MQ=255)

                                                  acCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGAtt                                       <  1:5802217/93‑1 (MQ=255)

                                                   ctgccgcGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGAGGGCTATGCCACCGATCCTCACTATGCCCGTAAACTCACCAACATGATTc                                      <  2:1615582/91‑1 (MQ=255)

                                                   ccgccgCGAGTGCGGAACAGGGGGCTCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTc                                      <  2:2782730/93‑1 (MQ=255)

                                                   ccgccgCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTc                                      <  1:4343851/93‑1 (MQ=255)

                                                    cgccgcGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTca                                     <  2:1010785/93‑1 (MQ=255)

                                                    cgccgcGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTca                                     >  2:762394/1‑93 (MQ=255)

                                                    cgccgcGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTca                                     <  1:1656380/93‑1 (MQ=255)

                                                     gccgcGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcag                                    >  2:3564334/1‑93 (MQ=255)

                                                     gccgcGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcag                                    >  1:2208160/1‑93 (MQ=255)

                                                        gcgAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTcagcag                                 >  2:5163350/1‑93 (MQ=255)

                                                          gAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGAt                               >  1:6222187/1‑93 (MQ=255)

                                                          gAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGAt                               >  1:868517/1‑93 (MQ=255)

                                                          gAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGAt                               <  1:375389/93‑1 (MQ=255)

                                                          gAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGAt                               <  2:472535/93‑1 (MQ=255)

                                                          gAGTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGAt                               <  2:4285510/93‑1 (MQ=255)

                                                            gTGCGGAACAGGGGGCGCAGGGCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGa                             >  2:5288303/1‑93 (MQ=255)

                                                            gTGCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGa                             >  2:3964781/1‑93 (MQ=255)

                                                             agCGGAACAGGGGGAGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGTCCGCATACTCAACAACATGATTTAGTACATGaa                            >  2:3081845/2‑93 (MQ=255)

                                                              gCGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGaaa                           <  2:4345654/93‑1 (MQ=255)

                                                               cGGAACAGGGTGCGCAGGCCCTACAGGGCGCGGTCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAAt                          <  2:487809/93‑1 (MQ=255)

                                                               cGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAAt                          <  1:5619601/93‑1 (MQ=255)

                                                               cGGAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAAt                          <  1:3130498/93‑1 (MQ=255)

                                                                ggAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATc                         <  2:4131311/93‑1 (MQ=255)

                                                                 gAACAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCg                        >  2:4961317/1‑93 (MQ=255)

                                                                  aaCAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGa                       <  1:5896791/93‑1 (MQ=255)

                                                                  aaCAGGGGGCGCAGGACCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGa                       >  1:974039/1‑93 (MQ=255)

                                                                   aCAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGAt                      >  1:2657255/1‑93 (MQ=255)

                                                                   aCAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGAt                      <  1:1937380/93‑1 (MQ=255)

                                                                   aCAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGAt                      >  1:2348553/1‑93 (MQ=255)

                                                                   aCAGGGAGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGAt                      <  2:5670226/93‑1 (MQ=255)

                                                                    cAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATa                     <  1:4967443/93‑1 (MQ=255)

                                                                    cAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATa                     <  2:5778306/93‑1 (MQ=255)

                                                                    cAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATa                     >  2:1950944/1‑93 (MQ=255)

                                                                    cAGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATa                     <  1:3631623/93‑1 (MQ=255)

                                                                     aGGGGGCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATaa                    <  1:3567118/93‑1 (MQ=255)

                                                                     aGGGCGCGCAGACCCTACGGGGCGCGGGCTATGCTGCAGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATaa                    <  2:5909395/93‑1 (MQ=255)

                                                                      gggggCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAg                   <  1:1645024/93‑1 (MQ=255)

                                                                       ggggCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGc                  <  1:2116196/93‑1 (MQ=255)

                                                                       ggggCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGc                  <  1:3223010/93‑1 (MQ=255)

                                                                        gggCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCg                 <  1:1786265/93‑1 (MQ=255)

                                                                        gggCGCAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCg                 <  1:3107207/93‑1 (MQ=255)

                                                                          gcgcAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGAc               <  1:5947460/93‑1 (MQ=255)

                                                                           cgcAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACa              <  2:659831/93‑1 (MQ=255)

                                                                           cgcAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACa              <  1:5520665/93‑1 (MQ=255)

                                                                            gcAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACaa             <  2:4501376/93‑1 (MQ=255)

                                                                            gcAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACaa             <  2:3694726/93‑1 (MQ=255)

                                                                            gcAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACaa             <  2:6279235/93‑1 (MQ=255)

                                                                            gcAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACaa             <  1:2576243/93‑1 (MQ=255)

                                                                             cAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAg            <  2:2911839/93‑1 (MQ=255)

                                                                             cAGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAg            <  1:3964044/93‑1 (MQ=255)

                                                                              aGGCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAgg           <  2:867777/93‑1 (MQ=255)

                                                                              aGGCCCTACAGGGCGCGGCCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAgg           <  2:2854319/93‑1 (MQ=255)

                                                                                gCCCTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTg         <  1:2463999/93‑1 (MQ=255)

                                                                                 cccTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGa        <  2:4016703/93‑1 (MQ=255)

                                                                                 cccTACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGa        >  1:1137038/1‑93 (MQ=255)

                                                                                    tACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCa     >  2:5079743/1‑93 (MQ=255)

                                                                                    tACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCa     <  2:6204968/93‑1 (MQ=255)

                                                                                    tACAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCa     >  1:4272210/1‑93 (MQ=255)

                                                                                     aCAGGGCGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCaa    <  2:1123628/93‑1 (MQ=255)

CGGATTACGTTGGGCTGTTAACGCGTAACCCGCGCTACGCCGCCGTGACGACCGCCGCGAGTGCGGAACAGGGGGCGCAGGCCCTACAGGACGCGGGCTATGCCACCGATCCTCACTATGCCCGCAAACTCACCAACATGATTCAGCAGATGAAATCGATAAGCGACAAGGTGAGCAAAA  >  NC_000913/1137324‑1137503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`