BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,144,672	A→G	K170K (AAA→AAG)	yceC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,144,672	0	A	G	99.5%	608.6 / ‑4.9	190	K170K (AAA→AAG)	yceC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (102/87); total (102/88)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

Only 100 of 208 total aligned reads displayed.

TCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCT  >  NC_000913/1144582‑1144761

tCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAggg                                                                                         <  2:5930339/93‑1 (MQ=255)

tCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAggg                                                                                         <  1:4794528/93‑1 (MQ=255)

 cATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGGTCTCTGCATGAGCAATTACGTGAAAAgggg                                                                                        >  1:2569539/1‑93 (MQ=255)

     gTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGc                                                                                    >  1:111318/1‑93 (MQ=255)

      tCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCa                                                                                   >  1:1145110/1‑93 (MQ=255)

      tCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCa                                                                                   >  2:276417/1‑93 (MQ=255)

      tCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCa                                                                                   <  2:3323773/93‑1 (MQ=255)

      gcTTGACCGGGACGCCTCAGGTGCGTTGCTGGTAACGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCa                                                                                   <  1:4356809/92‑1 (MQ=255)

       cTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaa                                                                                  >  2:4287502/1‑93 (MQ=255)

        ttGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaaa                                                                                 >  2:5654673/1‑93 (MQ=255)

        ttGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCaaa                                                                                 <  2:5282788/93‑1 (MQ=255)

           aCCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAg                                                                              >  1:5520810/1‑93 (MQ=255)

              gggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCCAAAAGAtt                                                                           <  2:6134618/93‑1 (MQ=255)

              gggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGAtt                                                                           >  1:6275370/1‑93 (MQ=255)

               ggACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTa                                                                          >  2:5033503/1‑93 (MQ=255)

                 acacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTAcc                                                                        <  2:5936779/93‑1 (MQ=255)

                  cacCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCt                                                                       <  2:4038202/93‑1 (MQ=255)

                   acCTCAGGTGTTTTGCTGGTAGCTAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                      <  1:954852/93‑1 (MQ=255)

                   acCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                      <  1:4642111/93‑1 (MQ=255)

                   acCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                      <  2:2393838/93‑1 (MQ=255)

                   acCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                      >  2:1147829/1‑93 (MQ=255)

                   acCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTg                                                                      >  1:1907805/1‑93 (MQ=255)

                    ccTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTgg                                                                     >  1:791735/1‑93 (MQ=255)

                     cTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                    <  2:6122205/93‑1 (MQ=255)

                     cTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgc                                                                    <  1:4574038/93‑1 (MQ=255)

                       cAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgcgc                                                                  <  1:166292/93‑1 (MQ=255)

                       cAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGgcgc                                                                  >  1:720970/1‑93 (MQ=255)

                        aGGTGTTTTGCTGGTAGCGAAAAAACGCTCGTCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCt                                                                 >  2:1401562/1‑93 (MQ=255)

                        aGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCt                                                                 >  1:2865076/1‑93 (MQ=255)

                        aGGTGTTTTGCTGCTAGCCAAAAAACGCTCGGCGCTGCGTTCTCTGCATGAGCAATTACGTGTAAAGGGGATGAAAAAAGCTTACCTGGCGCt                                                                 >  2:1600388/1‑93 (MQ=255)

                        aGGGGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGGGAAAAGGGGATGCAAAAAGATTACCTGGCGCt                                                                 >  2:2291921/1‑93 (MQ=255)

                         ggTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTg                                                                >  1:3230193/1‑93 (MQ=255)

                         ggTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTg                                                                >  2:2877707/1‑93 (MQ=255)

                         ggTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTg                                                                >  2:3915/1‑93 (MQ=255)

                          gtgtTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTgg                                                               >  2:1630480/1‑93 (MQ=255)

                             ttttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgc                                                            <  2:1340480/93‑1 (MQ=255)

                             ttttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgc                                                            <  1:5256824/93‑1 (MQ=255)

                               ttGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTgcgc                                                          >  1:4038343/1‑93 (MQ=255)

                                  cTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGt                                                       <  1:980742/93‑1 (MQ=255)

                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                   <  2:3260205/93‑1 (MQ=255)

                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                   >  2:317943/1‑93 (MQ=255)

                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                   <  1:4827339/93‑1 (MQ=255)

                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                   <  2:5175122/93‑1 (MQ=255)

                                      tAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGt                                                   <  1:3260853/93‑1 (MQ=255)

                                        gCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTgg                                                 <  2:2434545/93‑1 (MQ=255)

                                             aaaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTc                                            >  1:5905683/1‑93 (MQ=255)

                                             aaaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTc                                            <  1:4263284/93‑1 (MQ=255)

                                              aaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCg                                           >  2:353990/1‑93 (MQ=255)

                                              aaaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCg                                           >  1:6130728/1‑93 (MQ=255)

                                               aaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGc                                          >  1:5760506/1‑93 (MQ=255)

                                               aaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGc                                          >  1:5833954/1‑93 (MQ=255)

                                               aaCGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGc                                          <  1:221442/93‑1 (MQ=255)

                                                 cGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAt                                        <  2:5329226/93‑1 (MQ=255)

                                                  gCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtg                                       <  1:2460166/93‑1 (MQ=255)

                                                  gCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtg                                       >  2:2250901/1‑93 (MQ=255)

                                                   cTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGCTTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgt                                      >  2:6011367/1‑93 (MQ=255)

                                                   cTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgt                                      >  2:3658765/1‑93 (MQ=255)

                                                   cTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgg                                      >  2:315077/1‑92 (MQ=255)

                                                    tCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgtg                                     >  2:424905/1‑93 (MQ=255)

                                                    tCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCAtgtg                                     >  2:2849169/1‑93 (MQ=255)

                                                       gcgttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAag                                  <  1:2045543/93‑1 (MQ=255)

                                                         gttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAagag                                <  1:3195517/93‑1 (MQ=255)

                                                          ttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAagagg                               >  2:4387421/1‑92 (MQ=255)

                                                          ttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGc                               >  2:584158/1‑93 (MQ=255)

                                                          ttgcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGc                               >  2:467303/1‑93 (MQ=255)

                                                            tcgtcCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGt                             <  2:2556829/92‑1 (MQ=255)

                                                            gcgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGt                             <  1:3836508/93‑1 (MQ=255)

                                                             cgttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGtt                            >  1:2942101/1‑93 (MQ=255)

                                                               ttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCa                          >  1:2955092/1‑93 (MQ=255)

                                                               ttCTCTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCa                          <  1:1782425/93‑1 (MQ=255)

                                                                tctcTGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCaa                         <  2:2206506/93‑1 (MQ=255)

                                                                   ctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCa                      >  2:5007404/1‑93 (MQ=255)

                                                                   ctGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCa                      >  1:5093344/1‑93 (MQ=255)

                                                                    tGCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCAc                     <  1:4713689/93‑1 (MQ=255)

                                                                     gCATGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCAcc                    <  2:1727329/93‑1 (MQ=255)

                                                                        tGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                 >  1:1554/1‑93 (MQ=255)

                                                                        tGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                 >  2:814646/1‑93 (MQ=255)

                                                                        tGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                 >  2:3083562/1‑93 (MQ=255)

                                                                        tGAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                 >  1:3986430/1‑93 (MQ=255)

                                                                        tGAGCAATTACGTGAAAAGGGGATACAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGtt                 >  2:4802565/1‑93 (MQ=255)

                                                                         gAGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTa                >  1:5332838/1‑93 (MQ=255)

                                                                          aGCAATTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTAc               >  1:2368288/1‑93 (MQ=255)

                                                                              aTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaa           >  2:759485/1‑93 (MQ=255)

                                                                              aTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaa           >  1:3085405/1‑93 (MQ=255)

                                                                              aTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaa           >  1:4978858/1‑93 (MQ=255)

                                                                              aTTACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGCTCAAGCACCGTTACTGaa           >  2:6305806/1‑93 (MQ=255)

                                                                                tACGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaaa         <  1:5922571/93‑1 (MQ=255)

                                                                                 aCGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaaaa        >  2:2116198/1‑93 (MQ=255)

                                                                                 aCGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaaaa        <  1:3853450/93‑1 (MQ=255)

                                                                                 aCGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGaaaaa        <  1:3457962/93‑1 (MQ=255)

                                                                                  cGTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAat       <  1:2983392/93‑1 (MQ=255)

                                                                                   gTGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAata      <  2:1742899/93‑1 (MQ=255)

                                                                                    tGAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAatat     <  1:340409/93‑1 (MQ=255)

                                                                                     gAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATAtt    <  2:1954971/93‑1 (MQ=255)

                                                                                     gAAAAGGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATAtt    >  1:1414558/1‑93 (MQ=255)

                                                                                      aaaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTc   <  2:4567460/93‑1 (MQ=255)

                                                                                      aaaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTc   <  2:3745544/93‑1 (MQ=255)

                                                                                       aaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCt  <  1:1835179/93‑1 (MQ=255)

                                                                                       aaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCt  >  1:2743641/1‑93 (MQ=255)

                                                                                       aaagGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCt  <  1:228562/93‑1 (MQ=255)

TCATCGTCTTGACCGGGACACCTCAGGTGTTTTGCTGGTAGCGAAAAAACGCTCGGCGTTGCGTTCTCTGCATGAGCAATTACGTGAAAAAGGGATGCAAAAAGATTACCTGGCGCTGGTGCGCGGTCAGTGGCAGTCGCATGTGAAGAGCGTTCAAGCACCGTTACTGAAAAATATTCT  >  NC_000913/1144582‑1144761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`