BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,226,344	C→T	A118T (GCC→ACC)	ycgK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,226,344	0	C	T	99.5%	675.9 / ‑5.4	189	A118T (GCC→ACC)	ycgK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (105/82); total (107/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

Only 100 of 196 total aligned reads displayed.

TTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATC  >  NC_000913/1226255‑1226433

 tAACGTTTATGACGGGGAGAGACCCCGTCGGTTGTCATTTCCTTAATTTTTATATCGACGTTGTATTTTTTAGTTTTGTTTTTACGGGcgtcg                                                                                       >  2:1345701/1‑93 (MQ=255)

    cGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGttt                                                                                    <  2:3175553/93‑1 (MQ=255)

    cGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGttt                                                                                    <  2:2904870/93‑1 (MQ=255)

      tttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCg                                                                                  <  2:2238552/93‑1 (MQ=255)

       ttATGACGGGGAGAGTCTCCGGCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCgg                                                                                 <  2:247072/93‑1 (MQ=255)

       ttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCgg                                                                                 >  1:2983397/1‑93 (MQ=255)

       ttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCgg                                                                                 <  2:2163352/93‑1 (MQ=255)

       ttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCgg                                                                                 <  2:2698916/93‑1 (MQ=255)

       ttATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCgg                                                                                 >  1:6174656/1‑93 (MQ=255)

        tATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCggg                                                                                >  1:2203707/1‑93 (MQ=255)

         aTGACGGGNANAGTCCCCGTCGGTNGNCATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGt                                                                               <  2:380552/93‑1 (MQ=255)

            aCGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTg                                                                            <  1:5931248/93‑1 (MQ=255)

             cGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGc                                                                           >  1:5140117/1‑93 (MQ=255)

               gggAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCaa                                                                         >  1:4124527/1‑93 (MQ=255)

               gggAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCaa                                                                         >  1:3626246/1‑93 (MQ=255)

                ggAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAac                                                                        >  2:3544292/1‑93 (MQ=255)

                 gagaGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAaca                                                                       >  2:2038087/1‑93 (MQ=255)

                  agagTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAacac                                                                      <  2:3994629/93‑1 (MQ=255)

                  agagTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAacac                                                                      <  2:3728094/93‑1 (MQ=255)

                   gagTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACt                                                                     <  2:5221738/93‑1 (MQ=255)

                     gTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCg                                                                   >  1:2028939/1‑93 (MQ=255)

                      tCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGc                                                                  <  1:1333786/93‑1 (MQ=255)

                         ccGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGc                                                               >  1:3802851/1‑93 (MQ=255)

                             cGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCat                                                           >  1:5280112/1‑93 (MQ=255)

                              ggTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCata                                                          >  1:3397751/1‑93 (MQ=255)

                               gTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCatat                                                         >  2:589867/1‑93 (MQ=255)

                               gTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCatat                                                         >  2:6237650/1‑93 (MQ=255)

                               gTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCatat                                                         >  2:2154099/1‑93 (MQ=255)

                                ttGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATAtt                                                        <  2:2001330/93‑1 (MQ=255)

                                ttGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATAtt                                                        <  1:4921012/93‑1 (MQ=255)

                                ttGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATAtt                                                        >  2:297862/1‑93 (MQ=255)

                                ttGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATAtt                                                        >  1:932327/1‑93 (MQ=255)

                                ttGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATAtt                                                        <  1:6259959/93‑1 (MQ=255)

                                 tGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATAttt                                                       <  2:1666305/93‑1 (MQ=255)

                                  gACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTg                                                      >  1:828273/1‑93 (MQ=255)

                                   aCATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGc                                                     >  2:4461964/1‑93 (MQ=255)

                                    cATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGcc                                                    <  2:5735255/93‑1 (MQ=255)

                                     aTTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCa                                                   >  2:853041/1‑93 (MQ=255)

                                     aTTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCa                                                   >  2:4165557/1‑93 (MQ=255)

                                     aTTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCa                                                   >  1:1515684/1‑93 (MQ=255)

                                      tttACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAg                                                  >  2:3317870/1‑93 (MQ=255)

                                      tttACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAg                                                  >  2:1818786/1‑93 (MQ=255)

                                      tttACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAg                                                  >  1:5617545/1‑93 (MQ=255)

                                      tttACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAg                                                  >  1:1826113/1‑93 (MQ=255)

                                       ttACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGa                                                 >  2:5106760/1‑93 (MQ=255)

                                        tACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGaa                                                >  1:5749521/1‑93 (MQ=255)

                                         aCTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAg                                               <  2:727463/93‑1 (MQ=255)

                                          cTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGc                                              <  2:1252879/93‑1 (MQ=255)

                                           ttAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCa                                             >  1:1991680/1‑93 (MQ=255)

                                           ttAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCa                                             <  1:3742043/93‑1 (MQ=255)

                                           ttAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCa                                             <  1:4748535/93‑1 (MQ=255)

                                           ttAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTATGCGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCa                                             <  2:4282410/93‑1 (MQ=255)

                                             aaTTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAgg                                           <  1:1576931/93‑1 (MQ=255)

                                              aTTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAggg                                          <  2:4798842/93‑1 (MQ=255)

                                              aTTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAggg                                          >  1:2805788/1‑93 (MQ=255)

                                                 tGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagt                                       <  2:1543152/93‑1 (MQ=255)

                                                 tGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagt                                       <  2:345509/93‑1 (MQ=255)

                                                  gAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtg                                      <  1:4388765/93‑1 (MQ=255)

                                                    atatCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGgagtgag                                    >  1:260278/1‑93 (MQ=255)

                                                     tatCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGACAGAAGCAGGgagtgagt                                   >  1:1341271/1‑93 (MQ=255)

                                                      atCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTa                                  >  1:2680297/1‑93 (MQ=255)

                                                      atCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTa                                  >  2:5579817/1‑93 (MQ=255)

                                                       tCGACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTAt                                 <  2:3283297/93‑1 (MQ=255)

                                                         gACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTg                               >  2:3225036/1‑93 (MQ=255)

                                                         gACGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGGTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGGGAGTATTg                               >  2:4446029/1‑93 (MQ=255)

                                                          aCGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGt                              >  1:2180666/1‑93 (MQ=255)

                                                          aCGTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGGATTGt                              >  1:3921141/1‑93 (MQ=255)

                                                            gTTATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTcc                            <  1:3960864/93‑1 (MQ=255)

                                                             ttATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCg                           >  1:2879726/1‑93 (MQ=255)

                                                             ttATATTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCg                           <  2:3076364/93‑1 (MQ=255)

                                                              tataTTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGt                          >  2:2095869/1‑93 (MQ=255)

                                                              tataTTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATNTGCCAGAAGCAGGGAGTGAGTNTNGNCNGt                          >  1:779988/1‑93 (MQ=255)

                                                                tatTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGa                        >  1:5477365/1‑93 (MQ=255)

                                                                 atTTTTTAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGAc                       <  1:2448108/93‑1 (MQ=255)

                                                                  ttttttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACt                      >  2:3497948/1‑93 (MQ=255)

                                                                  ttttttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACt                      <  2:2752213/93‑1 (MQ=255)

                                                                  ttttttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACt                      >  1:3211046/1‑93 (MQ=255)

                                                                    ttttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTAt                    >  1:3547751/1‑93 (MQ=255)

                                                                    ttttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTAt                    <  1:6044751/93‑1 (MQ=255)

                                                                     tttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATc                   >  1:2289331/1‑93 (MQ=255)

                                                                     tttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATc                   >  1:226227/1‑93 (MQ=255)

                                                                     tttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGGGAGTATTGTCCGTGACTATc                   >  2:1952116/1‑93 (MQ=255)

                                                                      ttAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCg                  >  2:2191151/1‑93 (MQ=255)

                                                                       tAGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTAATGTCCGTGACTATCGa                 <  1:3613414/93‑1 (MQ=255)

                                                                        aGTTTTGTTTTTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAg                <  1:306366/93‑1 (MQ=255)

                                                                         gttttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGt               <  2:2633857/93‑1 (MQ=255)

                                                                         gttttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGt               <  2:4547301/93‑1 (MQ=255)

                                                                         gttttgttttTACGGGTGTCGTTTCGGGGTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGt               >  2:6030984/1‑93 (MQ=255)

                                                                          ttttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGtt              >  2:1759387/1‑93 (MQ=255)

                                                                            ttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCt            >  1:5322291/1‑93 (MQ=255)

                                                                            ttgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCt            <  1:1362678/93‑1 (MQ=255)

                                                                             tgttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTg           <  1:4049013/93‑1 (MQ=255)

                                                                              gttttTACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTgg          <  1:737024/93‑1 (MQ=255)

                                                                               tttttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTggg         <  1:4529231/93‑1 (MQ=255)

                                                                                ttttACGGGTGTCTTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTgggg        <  1:1778324/93‑1 (MQ=255)

                                                                                ttttACGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTgggg        >  1:4973066/1‑93 (MQ=255)

                                                                                   tACGGGTGTGGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAat     <  2:4394885/93‑1 (MQ=255)

                                                                                    aCGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAata    >  1:6222778/1‑93 (MQ=255)

                                                                                    aCGGGTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAata    <  1:1445323/93‑1 (MQ=255)

                                                                                      gggTGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATc  >  1:3476915/1‑93 (MQ=255)

TTAACGTTTATGACGGGGAGAGTCCCCGTCGGTTGACATTTACTTAATTTGAATATCGACGTTATATTTTTTAGTTTTGTTTTTACGGGCGTCGTTTCGGGTTTGCAACACTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATC  >  NC_000913/1226255‑1226433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`