BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	122,235	T→C	R48R (CGT→CGC)	pdhR →	transcriptional regulator for pyruvate dehydrogenase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	122,235	0	T	C	100.0%	771.0 / NA	242	R48R (CGT→CGC)	pdhR	transcriptional regulator for pyruvate dehydrogenase complex
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (137/105); total (137/105)

Only 100 of 250 total aligned reads displayed.

ACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGTCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCT  >  NC_000913/122145‑122324

aCTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGccc                                                                                         >  1:4207524/1‑93 (MQ=255)

  tGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCcct                                                                                       >  1:1529823/1‑93 (MQ=255)

   ggAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCcctc                                                                                      >  1:5192000/1‑93 (MQ=255)

     aGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCcctcct                                                                                    <  2:5481411/93‑1 (MQ=255)

        ttttGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGc                                                                                 <  1:325880/93‑1 (MQ=255)

        ttttGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGc                                                                                 <  2:4519261/93‑1 (MQ=255)

            gATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGa                                                                             >  1:1479129/1‑93 (MQ=255)

            gATCCTCGAACGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGa                                                                             <  1:1086937/93‑1 (MQ=255)

             aTCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAg                                                                            <  1:5158200/93‑1 (MQ=255)

             aTCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAg                                                                            <  2:6143914/93‑1 (MQ=255)

              tCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAgg                                                                           >  1:4726096/1‑93 (MQ=255)

               ccTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGc                                                                          >  2:1282315/1‑93 (MQ=255)

               ccTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGc                                                                          >  2:2000907/1‑93 (MQ=255)

               ccTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGc                                                                          >  1:4297900/1‑93 (MQ=255)

                cTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCg                                                                         >  2:3586224/1‑93 (MQ=255)

                cTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCACACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCg                                                                         >  1:744984/1‑93 (MQ=255)

                 tCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGa                                                                        <  1:1736533/93‑1 (MQ=255)

                  cGAGGGCACTCTCCGCCCGGGCGAAAACCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGAt                                                                       <  2:1258445/93‑1 (MQ=255)

                   gAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGAtt                                                                      >  2:2099054/1‑93 (MQ=255)

                   gAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGAtt                                                                      >  2:2390168/1‑93 (MQ=255)

                   gAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGAtt                                                                      >  2:3364992/1‑93 (MQ=255)

                   gAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGAtt                                                                      >  1:1905332/1‑93 (MQ=255)

                    aaGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTc                                                                     <  1:2938087/93‑1 (MQ=255)

                    aaGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTc                                                                     >  1:4531547/1‑93 (MQ=255)

                      ggCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCaa                                                                   <  1:795763/93‑1 (MQ=255)

                         aCTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGt                                                                >  2:3113366/1‑93 (MQ=255)

                          ctctCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtc                                                               >  2:3565176/1‑93 (MQ=255)

                           tctcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtct                                                              <  1:6192290/93‑1 (MQ=255)

                           tctcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtct                                                              >  1:5038530/1‑93 (MQ=255)

                            ctcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtctc                                                             >  2:153050/1‑93 (MQ=255)

                            ctcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtctc                                                             <  2:1773385/93‑1 (MQ=255)

                            ctcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtctc                                                             <  1:3518623/93‑1 (MQ=255)

                            ctcCGCCCGGCCGAAAAACTCCAACCGGAACGCGAACTGCCAAAACAGTTGGACGTCCCCCGCCCCTCCTTGCGTGAGGCGATTCAACGtctc                                                             <  1:3689843/93‑1 (MQ=255)

                             tcCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcg                                                            >  2:1882277/1‑93 (MQ=255)

                               cGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgac                                                          >  2:5549518/1‑92 (MQ=255)

                               cGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaa                                                          <  2:2752933/93‑1 (MQ=255)

                               cGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaa                                                          >  2:4392265/1‑93 (MQ=255)

                                gCCCGGGTGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaag                                                         >  1:287952/1‑93 (MQ=255)

                                 cccGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagc                                                        >  1:2174683/1‑93 (MQ=255)

                                 cccGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagc                                                        >  1:6158330/1‑93 (MQ=255)

                                  ccGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcg                                                       >  2:1009309/1‑93 (MQ=255)

                                    gggCGAAAACCTCCCACCGGAAGGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcgaa                                                     <  1:233013/93‑1 (MQ=255)

                                     ggCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcgaag                                                    <  2:4760187/93‑1 (MQ=255)

                                     ggCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcgaag                                                    <  1:4743611/93‑1 (MQ=255)

                                     ggCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTcgaagcgaag                                                    >  1:904053/1‑93 (MQ=255)

                                      gcaaaaacCTCCCACCGGACCGCGACCTGGCAAAACATTTTGACGTCCCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAgg                                                   <  2:5316233/90‑1 (MQ=255)

                                      gCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAgg                                                   <  1:5363293/93‑1 (MQ=255)

                                        gAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGc                                                 >  1:306522/1‑93 (MQ=255)

                                         aaaaaCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCt                                                >  1:1702576/1‑93 (MQ=255)

                                         aaaaaCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCt                                                >  1:1007761/1‑93 (MQ=255)

                                          aagccTCCCACCGGATCGCGAACTGGCAAAACGGTTTCACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCtt                                               <  1:2281528/89‑1 (MQ=255)

                                          aaaaCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCtt                                               <  1:222923/93‑1 (MQ=255)

                                           aaaCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCttg                                              >  2:4058654/1‑93 (MQ=255)

                                            accTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCttgt                                             <  1:5593055/91‑1 (MQ=255)

                                            aaCTCCCACCGGAACGCGAACTGGCAAAACAGTTGGACGTCCCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCttgt                                             <  1:674846/93‑1 (MQ=255)

                                             aCTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCttgtt                                            >  2:1385218/1‑93 (MQ=255)

                                              cTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCttgttg                                           <  2:2228764/93‑1 (MQ=255)

                                               tCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGc                                          >  1:4410584/1‑93 (MQ=255)

                                                cccACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCt                                         >  1:5091810/1‑93 (MQ=255)

                                                cccACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCt                                         >  1:2266361/1‑93 (MQ=255)

                                                cccACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCGTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCt                                         >  1:3613666/1‑93 (MQ=255)

                                                  cACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtc                                       <  1:2724104/93‑1 (MQ=255)

                                                    ccGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcgt                                     <  2:4637159/93‑1 (MQ=255)

                                                    ccGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcgt                                     >  2:4839719/1‑93 (MQ=255)

                                                    ccGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcgt                                     <  1:5923550/93‑1 (MQ=255)

                                                     cGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcgtc                                    <  1:587183/93‑1 (MQ=255)

                                                     cGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcgtc                                    <  1:504767/93‑1 (MQ=255)

                                                      ggAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGTGGCGATTCAACGTCTCGAAGCGAAGGGCTTCGTGCTtggtcg                                   >  2:3909562/1‑93 (MQ=255)

                                                      ggAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTtcgtcg                                   >  2:5832966/1‑93 (MQ=255)

                                                        aaCGCGAAGTGGCAAAACACTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGcc                                 >  2:6163061/1‑93 (MQ=255)

                                                        aaCGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGcc                                 >  2:5097743/1‑93 (MQ=255)

                                                        aaCGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGcc                                 >  2:5978295/1‑93 (MQ=255)

                                                         aCGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCa                                <  1:2060126/93‑1 (MQ=255)

                                                         aCGCGAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCa                                <  1:2550114/93‑1 (MQ=255)

                                                          cgcgAACTGGCAAAACAGTTTGCCGCCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAg                               <  1:696095/93‑1 (MQ=255)

                                                           gcgAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAgg                              >  1:6043856/1‑93 (MQ=255)

                                                           gcgAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAgg                              >  2:3709966/1‑93 (MQ=255)

                                                            cgAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAggg                             <  1:2906653/93‑1 (MQ=255)

                                                             gAACTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAgggg                            >  2:2463791/1‑92 (MQ=255)

                                                              aaCTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAgggcg                           >  2:770124/1‑91 (MQ=255)

                                                              aaCTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTg                           <  1:962209/93‑1 (MQ=255)

                                                              aaCTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTg                           <  2:2659524/93‑1 (MQ=255)

                                                                cTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggc                         <  1:931290/93‑1 (MQ=255)

                                                                cTGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggc                         <  2:5670657/93‑1 (MQ=255)

                                                                 tGGCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcg                        >  1:4936641/1‑93 (MQ=255)

                                                                   gCAAAACAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTggcggc                      >  1:3990525/1‑93 (MQ=255)

                                                                        aCAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACttt                 <  1:1332360/93‑1 (MQ=255)

                                                                         cAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACtttt                >  2:3305837/1‑93 (MQ=255)

                                                                         cAGTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACtttt                >  1:1266211/1‑93 (MQ=255)

                                                                           gTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGt              >  2:5227341/1‑93 (MQ=255)

                                                                           gTTTGACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGGGGCGGCACTTTTGt              >  1:3813178/1‑93 (MQ=255)

                                                                              tGACGGCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCa           <  2:5727783/93‑1 (MQ=255)

                                                                               gACGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCag          <  2:2176264/93‑1 (MQ=255)

                                                                                aCGTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCaga         <  1:3359485/93‑1 (MQ=255)

                                                                                  gTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGagc       >  1:4291342/1‑93 (MQ=255)

                                                                                  gTCTCCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGagc       >  1:647979/1‑93 (MQ=255)

                                                                                    ctcCCGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGagcag     >  1:3893371/1‑93 (MQ=255)

                                                                                      cccGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGcc   >  2:537594/1‑93 (MQ=255)

                                                                                      cccGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGcc   <  2:42002/93‑1 (MQ=255)

                                                                                       ccGCCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCt  >  2:2581663/1‑93 (MQ=255)

ACTGGAGTTTTTGATCCTCGAAGGCACTCTCCGCCCGGGCGAAAAACTCCCACCGGAACGCGAACTGGCAAAACAGTTTGACGTCTCCCGTCCCTCCTTGCGTGAGGCGATTCAACGTCTCGAAGCGAAGGGCTTGTTGCTTCGTCGCCAGGGTGGCGGCACTTTTGTCCAGAGCAGCCT  >  NC_000913/122145‑122324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`