BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,303,961	A→G	E58E (GAA→GAG)	oppF →	OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,303,961	0	A	G	99.5%	641.9 / NA	201	E58E (GAA→GAG)	oppF	OppF
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (105/95); total (105/96)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 206 total aligned reads displayed.

GTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTT  >  NC_000913/1303871‑1304050

 tGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCg                                                                                        >  1:3948345/1‑93 (MQ=255)

 tGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCg                                                                                        <  2:3258152/93‑1 (MQ=255)

   gTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCggg                                                                                      <  2:2605908/93‑1 (MQ=255)

   gTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCggg                                                                                      >  2:1442/1‑93 (MQ=255)

    ttCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGa                                                                                     <  2:5761798/93‑1 (MQ=255)

    ttCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGa                                                                                     >  1:5340407/1‑93 (MQ=255)

    tgcTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGa                                                                                     <  2:1093664/91‑1 (MQ=255)

     tCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGAt                                                                                    >  1:2477466/1‑93 (MQ=255)

       tGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGc                                                                                  <  1:5307251/93‑1 (MQ=255)

       tGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGc                                                                                  >  1:2420177/1‑93 (MQ=255)

        ggCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCg                                                                                 >  1:5044423/1‑93 (MQ=255)

           aaCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTa                                                                              >  1:4833243/1‑93 (MQ=255)

           aaCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTa                                                                              >  2:5011867/1‑93 (MQ=255)

            aCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTaa                                                                             >  1:62937/1‑93 (MQ=255)

            aCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTaa                                                                             >  2:5143499/1‑93 (MQ=255)

            aCCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTaa                                                                             <  1:1737277/93‑1 (MQ=255)

             ccgccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAg                                                                            >  1:3416305/1‑93 (MQ=255)

             ccgccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAg                                                                            <  2:395999/93‑1 (MQ=255)

               gccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTc                                                                          <  2:3616402/93‑1 (MQ=255)

               gccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTc                                                                          >  1:1939275/1‑93 (MQ=255)

                ccgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTcc                                                                         >  1:4557637/1‑93 (MQ=255)

                 cgAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCa                                                                        >  1:2688882/1‑93 (MQ=255)

                  gAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGATACATTAGGTGTGGTAGGGGAGTCGGGGTGCGGTAAGTCCAc                                                                       >  1:361334/1‑93 (MQ=255)

                  gAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCGAc                                                                       >  1:5445333/1‑93 (MQ=255)

                  gAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCAc                                                                       <  2:1575775/93‑1 (MQ=255)

                   aaaaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCAcc                                                                      >  1:5569058/1‑93 (MQ=255)

                   aaaaCGCTCAAAGCCGTCGATGGTCTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCAcc                                                                      <  2:101337/93‑1 (MQ=255)

                     aaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCtt                                                                    <  1:4177727/93‑1 (MQ=255)

                     aaCGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCtt                                                                    <  1:4425824/93‑1 (MQ=255)

                       cGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTg                                                                  >  1:2097033/1‑93 (MQ=255)

                       cGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTg                                                                  <  2:2325545/93‑1 (MQ=255)

                       cGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTg                                                                  <  1:3185487/93‑1 (MQ=255)

                        gCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGc                                                                 <  2:4780887/93‑1 (MQ=255)

                        gCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGc                                                                 <  2:488135/93‑1 (MQ=255)

                         cTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGCAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCt                                                                >  2:3413963/1‑93 (MQ=255)

                         cTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGTGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCt                                                                >  2:3058632/1‑93 (MQ=255)

                          tCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTc                                                               >  2:1717497/1‑93 (MQ=255)

                          tCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTc                                                               <  2:2427958/93‑1 (MQ=255)

                          tCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTc                                                               >  1:3288421/1‑93 (MQ=255)

                          tCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTc                                                               >  1:3144588/1‑93 (MQ=255)

                           cAAAGCCGACGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTcg                                                              >  2:6130197/1‑93 (MQ=255)

                           cAAAGCCATCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTcg                                                              >  1:1644642/1‑93 (MQ=255)

                            aaaGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTcgc                                                             >  2:6201099/1‑93 (MQ=255)

                                 cGTCGATGGTGTCACTCTTCGCCTGTCTGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcat                                                        >  2:5210635/1‑93 (MQ=255)

                                 cGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcat                                                        >  2:6180403/1‑93 (MQ=255)

                                 cGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcat                                                        >  2:2060778/1‑93 (MQ=255)

                                  gTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatc                                                       <  2:381315/93‑1 (MQ=255)

                                   tCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatca                                                      <  1:5766515/93‑1 (MQ=255)

                                   tCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCcatca                                                      >  2:1915116/1‑93 (MQ=255)

                                     gATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCatcatc                                                    >  2:2473863/1‑93 (MQ=255)

                                      aTGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCg                                                   >  1:5897035/1‑93 (MQ=255)

                                       tGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCgg                                                  >  2:4059512/1‑93 (MQ=255)

                                        ggTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGt                                                 <  1:2983682/93‑1 (MQ=255)

                                        ggTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGt                                                 >  2:36017/1‑93 (MQ=255)

                                        ggTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGt                                                 >  1:364005/1‑93 (MQ=255)

                                        ggTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGt                                                 >  2:5447704/1‑93 (MQ=255)

                                         gtgtCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGtt                                                <  2:337002/93‑1 (MQ=255)

                                            tCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTgg                                             >  1:3484378/1‑93 (MQ=255)

                                             cACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGt                                            <  2:2367865/93‑1 (MQ=255)

                                                tctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCaa                                         <  2:5884798/93‑1 (MQ=255)

                                                tctTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCaa                                         <  2:3557616/93‑1 (MQ=255)

                                                tctTCGCCTGTATGAAGGGGAAAAATTAGGTGGGGTAGGGGAGTCGGGCTGCGGCAAGTCCACCTTTGCTCGCGCCATCATCGGTTTggctaa                                         >  2:1799453/1‑89 (MQ=255)

                                                  ttCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAgg                                       <  2:299915/93‑1 (MQ=255)

                                                     gCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcga                                    <  2:3670294/93‑1 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgac                                   >  1:4495405/1‑93 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgac                                   >  2:3174909/1‑93 (MQ=255)

                                                      ccTGTATGAAGGGGAAACATTAGGAGTGGTAGGGGAGTCGGGATGCGGTAAGTTCACCTTTGCTCGCGCCATCATCGGGTTGGTCAACGcgac                                   >  2:6014776/1‑93 (MQ=255)

                                                       cTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgacc                                  >  1:1420191/1‑93 (MQ=255)

                                                        tGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccg                                 >  1:5966862/1‑93 (MQ=255)

                                                         gTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccga                                <  2:4986805/93‑1 (MQ=255)

                                                         gTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccga                                <  2:1150013/93‑1 (MQ=255)

                                                         gTATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccga                                >  1:5112389/1‑93 (MQ=255)

                                                          tATGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGcgaccgac                               >  1:3255459/1‑93 (MQ=255)

                                                           aTGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACg                              <  2:2280645/93‑1 (MQ=255)

                                                            tGAAGGGGAAACCGTAGGCGTGGTAGGGGAGCCGGGCTGCGGGAAGTTCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACgg                             >  2:5634076/1‑93 (MQ=255)

                                                            tGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACgg                             >  1:816028/1‑93 (MQ=255)

                                                            tGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACgg                             >  1:4024549/1‑93 (MQ=255)

                                                            tGAAGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACgg                             >  1:718582/1‑93 (MQ=255)

                                                               aGGGGAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGGTTGGTGAAGGCGACCGACGGTCa                          >  2:1203469/1‑93 (MQ=255)

                                                                ggggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCAt                         >  1:5535801/1‑93 (MQ=255)

                                                                  ggAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGt                       <  1:376197/93‑1 (MQ=255)

                                                                   gAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGtt                      >  1:3454952/1‑93 (MQ=255)

                                                                   gAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGtt                      >  2:148759/1‑93 (MQ=255)

                                                                   gAAACATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGtt                      <  2:4967731/93‑1 (MQ=255)

                                                                      aCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                   >  2:448522/1‑93 (MQ=255)

                                                                      aCATTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGcc                   <  2:5188923/93‑1 (MQ=255)

                                                                        aTTAGGTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTg                 <  1:1182229/93‑1 (MQ=255)

                                                                        aTTAGGTGTGGTAGGGGAGTCGAGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTg                 <  2:4342890/93‑1 (MQ=255)

                                                                            ggTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTa             <  1:4906928/93‑1 (MQ=255)

                                                                            ggTGTGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTa             <  2:213432/93‑1 (MQ=255)

                                                                             gtgtGGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAg            <  1:1198628/93‑1 (MQ=255)

                                                                              tgtgGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAgg           >  1:2558043/1‑93 (MQ=255)

                                                                                tgGTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTa         <  1:3014331/93‑1 (MQ=255)

                                                                                  gTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTaaa       >  1:4632486/1‑93 (MQ=255)

                                                                                  gTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTaaa       >  2:4400572/1‑93 (MQ=255)

                                                                                  gTAGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTaaa       >  1:891356/1‑93 (MQ=255)

                                                                                    aGGGGAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAaga     <  2:285367/93‑1 (MQ=255)

                                                                                     ggggAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAagag    >  2:1987651/1‑93 (MQ=255)

                                                                                     ggggAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAagag    >  2:5849919/1‑93 (MQ=255)

                                                                                       ggAGTCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGtt  <  2:2226470/93‑1 (MQ=255)

GTGGTTCTGGCAACCGCCGAAAACGCTCAAAGCCGTCGATGGTGTCACTCTTCGCCTGTATGAAGGGGAAACATTAGGTGTGGTAGGGGAATCGGGATGCGGTAAGTCCACCTTTGCTCGCGCCATCATCGGTTTGGTCAAGGCGACCGACGGTCATGTTGCCTGGTTAGGTAAAGAGTT  >  NC_000913/1303871‑1304050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`