BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,401,497	T→C	I365V (ATT→GTT)	ydaJ ←	putative aminohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,401,497	0	T	C	99.5%	658.5 / NA	205	I365V (ATT→GTT)	ydaJ	putative aminohydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (100/104); minor base A (1/0); total (101/104)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

CCACGTAGGAGGCTTGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAATGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACC  >  NC_000913/1401407‑1401584

ccacGTAGGAGGCTTGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACgg                                                                                       >  1:5099811/1‑93 (MQ=255)

ccacGTAGGAGGCTTGCCCTTGACGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACgg                                                                                       >  2:5070659/1‑93 (MQ=255)

ccacGTAGGAGGCGTGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACgg                                                                                       <  2:2237271/93‑1 (MQ=255)

   cGTAGGAGGCTTGCCCTTGATATTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCt                                                                                    <  2:4705586/93‑1 (MQ=255)

    gTAGGAGGCTTGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTg                                                                                   >  2:4483540/1‑93 (MQ=255)

     tAGGAGGCTTGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGa                                                                                  >  1:3199696/1‑93 (MQ=255)

     tAGGAGGCTTGCCCTTGATGTTGCTGCACGCGCGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGa                                                                                  >  1:2784453/1‑93 (MQ=255)

       ggaggCTTGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGAtt                                                                                <  2:2714622/93‑1 (MQ=255)

       ggaggCTTGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGAtt                                                                                >  2:842162/1‑93 (MQ=255)

       ggaggCTTGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGAtt                                                                                <  1:37953/93‑1 (MQ=255)

       ggaggCTTGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGACTGAtt                                                                                >  1:2940436/1‑93 (MQ=255)

        gaggCTTGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCTGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTg                                                                               >  2:3502939/1‑93 (MQ=255)

             ttGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGAcccc                                                                          >  2:2570009/1‑93 (MQ=255)

             ttGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGAcccc                                                                          <  1:4681898/93‑1 (MQ=255)

              tGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGAccccc                                                                         <  2:750605/93‑1 (MQ=255)

                   ttGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGAc                                                                    >  2:1491725/1‑93 (MQ=255)

                    tGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGAcc                                                                   >  1:4819104/1‑93 (MQ=255)

                       tGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCTTGATTGACCCCCGCGACCTGa                                                                <  1:424487/93‑1 (MQ=255)

                       tGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGa                                                                >  1:3494292/1‑93 (MQ=255)

                        gTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAg                                                               <  1:6107397/93‑1 (MQ=255)

                         ttGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAgc                                                              >  1:3394800/1‑93 (MQ=255)

                         ttGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAgc                                                              >  2:3518202/1‑93 (MQ=255)

                            ctgcACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAgccgc                                                           >  2:5620577/1‑93 (MQ=255)

                            ctgcACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAgccgc                                                           <  2:2916422/93‑1 (MQ=255)

                             tgcACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAgccgcc                                                          <  1:182567/93‑1 (MQ=255)

                             tgcACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAgccgcc                                                          >  1:2524422/1‑93 (MQ=255)

                             tgcACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAgccgcc                                                          <  1:83345/93‑1 (MQ=255)

                             tgcACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAgccgcc                                                          <  1:4795314/93‑1 (MQ=255)

                             tgcACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAAAGGCCTGATTGAACCCCGCGACCTGAgccgcc                                                          >  2:5531243/1‑93 (MQ=255)

                              gcACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCt                                                         <  2:4148422/93‑1 (MQ=255)

                               cACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTg                                                        >  1:1106630/1‑93 (MQ=255)

                                aCGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGa                                                       <  2:1977450/93‑1 (MQ=255)

                                 cgcgGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGAc                                                      <  2:2497623/93‑1 (MQ=255)

                                  ccgGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACt                                                     <  1:2171462/92‑1 (MQ=255)

                                    gggCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACttt                                                   <  1:1471804/93‑1 (MQ=255)

                                    gggCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACttt                                                   <  2:1034947/93‑1 (MQ=255)

                                       ccATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCa                                                <  1:184812/93‑1 (MQ=255)

                                       ccATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCa                                                >  2:3187659/1‑93 (MQ=255)

                                         atcatTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAAc                                              >  2:2635178/1‑93 (MQ=255)

                                           catTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCa                                            >  2:5301709/1‑93 (MQ=255)

                                           catTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCa                                            <  1:4055326/93‑1 (MQ=255)

                                           catTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCa                                            <  2:4791479/93‑1 (MQ=255)

                                            atTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACTGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCAt                                           >  2:6203726/1‑93 (MQ=255)

                                            atTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCAt                                           <  1:3977290/93‑1 (MQ=255)

                                            atTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCAt                                           >  1:5528372/1‑93 (MQ=255)

                                            atTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCAt                                           <  2:1328385/93‑1 (MQ=255)

                                            atTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCAt                                           >  2:4207704/1‑93 (MQ=255)

                                             ttAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATg                                          >  1:1929167/1‑93 (MQ=255)

                                             ttAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATg                                          >  1:2663126/1‑93 (MQ=255)

                                              tAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGc                                         <  2:418671/93‑1 (MQ=255)

                                               aaTGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCg                                        >  1:1822950/1‑93 (MQ=255)

                                                aTGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGa                                       >  2:4445327/1‑93 (MQ=255)

                                                aTGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGa                                       >  2:3062526/1‑93 (MQ=255)

                                                 tgtgGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGAc                                      >  1:1840114/1‑93 (MQ=255)

                                                 tgtgGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGAc                                      <  2:1546604/93‑1 (MQ=255)

                                                   tgGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGAccc                                    >  2:116091/1‑93 (MQ=255)

                                                    ggCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCa                                   <  1:86508/93‑1 (MQ=255)

                                                    ggCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCa                                   >  1:2505156/1‑93 (MQ=255)

                                                    ggCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCa                                   >  1:2693476/1‑93 (MQ=255)

                                                     gCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGCCCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCAt                                  <  2:534416/93‑1 (MQ=255)

                                                     gCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCAt                                  <  1:446141/93‑1 (MQ=255)

                                                     gCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCAt                                  >  2:202767/1‑93 (MQ=255)

                                                      cATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACAGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCAtt                                 <  2:5850757/93‑1 (MQ=255)

                                                        tCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTgc                               <  1:1502158/93‑1 (MQ=255)

                                                        tCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTgc                               <  1:5058176/93‑1 (MQ=255)

                                                        tCTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTCAGCCGCCTGACTTTGCAACCATGCGACCACTTgc                               >  2:1893831/1‑93 (MQ=255)

                                                         cTTCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTgcg                              >  1:3658796/1‑93 (MQ=255)

                                                          ttCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTgcgg                             >  1:4940840/1‑93 (MQ=255)

                                                          ttCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTgcgg                             >  2:5203003/1‑93 (MQ=255)

                                                           tCGGAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTgcggc                            <  1:6294012/93‑1 (MQ=255)

                                                             ggAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGa                          <  2:1105509/93‑1 (MQ=255)

                                                              gAACCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGaa                         >  2:3088382/1‑93 (MQ=255)

                                                                aCCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGGCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAgg                       >  1:1347364/1‑93 (MQ=255)

                                                                aCCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAgg                       <  1:2734940/93‑1 (MQ=255)

                                                                aCCCGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAgg                       >  2:5155971/1‑93 (MQ=255)

                                                                  ccGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGgag                     <  2:3711770/93‑1 (MQ=255)

                                                                   cGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGNNANCNTGCGACCCATTGCGGNGAAGgaga                    >  2:4892525/1‑93 (MQ=255)

                                                                   cGCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGgaga                    <  1:1255791/93‑1 (MQ=255)

                                                                    gCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGaa                   <  2:6260283/93‑1 (MQ=255)

                                                                    gCAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGaa                   <  1:3859746/93‑1 (MQ=255)

                                                                     cAGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAAc                  <  1:6195299/93‑1 (MQ=255)

                                                                      aGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACt                 >  1:5318426/1‑93 (MQ=255)

                                                                      aGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACt                 >  2:1250230/1‑93 (MQ=255)

                                                                      aGGCGCTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACt                 <  1:4130130/93‑1 (MQ=255)

                                                                        gcgcTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTgg               <  2:1798443/93‑1 (MQ=255)

                                                                        gcgcTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTgg               >  2:3531357/1‑93 (MQ=255)

                                                                         cgcTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGc              >  2:380538/1‑93 (MQ=255)

                                                                          gcTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCg             <  2:4260889/93‑1 (MQ=255)

                                                                          gcTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCg             >  1:2062025/1‑93 (MQ=255)

                                                                          gcTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCg             >  2:524780/1‑93 (MQ=255)

                                                                           cTTCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCgg            >  1:4395665/1‑93 (MQ=255)

                                                                            ttCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGt           <  1:2399015/93‑1 (MQ=255)

                                                                             tCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTa          >  2:770930/1‑93 (MQ=255)

                                                                             tCAACACGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTa          <  2:5235476/93‑1 (MQ=255)

                                                                                acacGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCt       >  1:2933688/1‑93 (MQ=255)

                                                                                acacGTACAGCGGCCAGAATGACCGCCGCGGCCTGAACCACCTGACTTTGCAACCATGCGACCCATTGCGACATAGGAGAACTGGCGGTAGCt       <  2:6304002/93‑1 (MQ=255)

                                                                                  acGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGc     >  2:5451191/1‑93 (MQ=255)

                                                                                   cGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCa    >  1:6046936/1‑93 (MQ=255)

                                                                                   cGTTCAACGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGGAGCTGCa    >  1:3065487/1‑93 (MQ=255)

                                                                                     ttCAACGGCCTGATTGACCCCCGCGACCTCAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGGAcc  >  1:5891184/1‑93 (MQ=255)

CCACGTAGGAGGCTTGCCCTTGATGTTGCTGCACGCGGGCCATCATTAATGTGGCATCTTCGGAACCCGCAGGCGCTTCAACACGTTCAATGGCCTGATTGACCCCCGCGACCTGAGCCGCCTGACTTTGCAACCATGCGACCCATTGCGGCGAAGGAGAACTGGCGGTAGCTGCACC  >  NC_000913/1401407‑1401584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`