BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	158,711	C→T	R128H (CGC→CAC)	pcnB ←	poly(A) polymerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	158,711	0	C	T	100.0%	846.5 / NA	232	R128H (CGC→CAC)	pcnB	poly(A) polymerase I
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (122/110); total (122/110)

Only 100 of 244 total aligned reads displayed.

ATACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATGA  >  NC_000913/158620‑158800

 tACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCgtgt                                                                                         <  1:1349799/93‑1 (MQ=255)

 tACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCgtgt                                                                                         >  1:1439445/1‑93 (MQ=255)

 tACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCgtgt                                                                                         >  2:4459559/1‑93 (MQ=255)

 tACAGGATGTTGATAGTGAAATCGCGGCGCTGGGCGTGTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCgtgt                                                                                         <  1:1881252/93‑1 (MQ=255)

   cAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTg                                                                                       >  1:6108446/1‑93 (MQ=255)

   cAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTg                                                                                       <  1:1854984/93‑1 (MQ=255)

     ggCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTggg                                                                                     <  1:4070738/93‑1 (MQ=255)

     ggCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTggg                                                                                     >  2:2487652/1‑93 (MQ=255)

      gCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGa                                                                                    <  1:5882649/93‑1 (MQ=255)

      gCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGa                                                                                    <  1:2199339/93‑1 (MQ=255)

       cTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAg                                                                                   >  2:4995532/1‑93 (MQ=255)

        tGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAgg                                                                                  >  1:148897/1‑93 (MQ=255)

         gTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGt                                                                                 >  2:6175815/1‑93 (MQ=255)

           tGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGCTGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGCTTTGCCCGTGTTGGGAGGGCg                                                                               >  2:4370321/1‑93 (MQ=255)

              tAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGc                                                                            >  1:4768152/1‑93 (MQ=255)

              tAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGc                                                                            <  2:4553298/93‑1 (MQ=255)

               aGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCg                                                                           <  2:1781806/93‑1 (MQ=255)

                gTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCgg                                                                          <  2:1331655/93‑1 (MQ=255)

                gTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCgg                                                                          <  1:1157201/93‑1 (MQ=255)

                gTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGCTGGGAGGTCGTGCgg                                                                          >  2:2096091/1‑93 (MQ=255)

                 tGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGt                                                                         <  1:1565647/93‑1 (MQ=255)

                  gAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTc                                                                        >  2:5174427/1‑93 (MQ=255)

                    aaTCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGc                                                                      <  1:3261355/93‑1 (MQ=255)

                     aTCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCt                                                                     >  2:2379791/1‑93 (MQ=255)

                      tCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTg                                                                    <  1:1040140/93‑1 (MQ=255)

                        gcggcgCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGACCGTGTTGGGAGGTCGTGCGGTCGCTGAc                                                                  >  1:3929282/1‑93 (MQ=255)

                              cTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTAcc                                                            <  2:5580350/93‑1 (MQ=255)

                               tGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCt                                                           <  1:2527773/93‑1 (MQ=255)

                                gggCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCtt                                                          <  1:5194324/93‑1 (MQ=255)

                                 ggCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTc                                                         >  2:3949665/1‑93 (MQ=255)

                                 ggCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTc                                                         <  1:4340873/93‑1 (MQ=255)

                                 ggCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTc                                                         <  1:2355002/93‑1 (MQ=255)

                                   cGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgt                                                       <  1:5081739/93‑1 (MQ=255)

                                     tcttctTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtgg                                                     >  1:6061828/1‑93 (MQ=255)

                                     tcttctTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtgg                                                     >  1:2122593/1‑93 (MQ=255)

                                      cttcttCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtggt                                                    >  2:5205553/1‑93 (MQ=255)

                                      cgtctgcGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtggt                                                    <  2:6093048/91‑1 (MQ=255)

                                       ttcttcGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCgtggtg                                                   >  1:759779/1‑93 (MQ=255)

                                        tcttcGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGgtgt                                                  >  1:3180571/1‑93 (MQ=255)

                                        tcttcGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGgtgt                                                  >  1:5922080/1‑93 (MQ=255)

                                          ttcGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTcc                                                >  1:2722871/1‑93 (MQ=255)

                                           tcGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCa                                               >  1:903023/1‑93 (MQ=255)

                                             gATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACg                                             <  1:3426296/93‑1 (MQ=255)

                                              aTGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACgg                                            <  1:3241174/93‑1 (MQ=255)

                                              aTGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACgg                                            >  1:4413773/1‑93 (MQ=255)

                                               tGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGa                                           >  2:3464957/1‑93 (MQ=255)

                                                ggAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGaa                                          <  2:1007227/93‑1 (MQ=255)

                                                ggAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGaa                                          >  2:145117/1‑93 (MQ=255)

                                                 gAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAg                                         <  2:5558204/93‑1 (MQ=255)

                                                  aGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAgg                                        >  2:3988241/1‑93 (MQ=255)

                                                   gCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGt                                       >  1:5520395/1‑93 (MQ=255)

                                                   gCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGt                                       >  1:4293133/1‑93 (MQ=255)

                                                    ccGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTc                                      <  2:672943/93‑1 (MQ=255)

                                                      gAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGc                                    >  2:3680443/1‑93 (MQ=255)

                                                      gAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGc                                    >  2:5428114/1‑93 (MQ=255)

                                                      gAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGc                                    >  1:368438/1‑93 (MQ=255)

                                                        aaaTGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCaa                                  <  2:3750971/93‑1 (MQ=255)

                                                        aaaTGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCaa                                  <  1:2908206/93‑1 (MQ=255)

                                                         aaTGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAAc                                 >  2:5388088/1‑93 (MQ=255)

                                                          aTGTTGTCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACt                                >  2:826221/1‑93 (MQ=255)

                                                           tgttgtCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCCACtt                               >  1:3145369/1‑93 (MQ=255)

                                                           tgttgtCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACtt                               >  1:968583/1‑93 (MQ=255)

                                                            gttgtCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTc                              >  2:1921127/1‑93 (MQ=255)

                                                             ttgtCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCg                             <  2:4133495/93‑1 (MQ=255)

                                                                tCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATa                          >  2:148900/1‑93 (MQ=255)

                                                                tCGCGCAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATa                          <  2:1200141/93‑1 (MQ=255)

                                                                  ccgcAGCAACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAAt                        <  2:573540/92‑1 (MQ=255)

                                                                    gcagcaACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAAtct                      >  1:6264335/1‑93 (MQ=255)

                                                                    gcagcaACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAAtct                      >  2:3105012/1‑93 (MQ=255)

                                                                     cagcaACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAAtctc                     >  2:1696192/1‑93 (MQ=255)

                                                                     cagcaACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAAtctc                     >  2:3074009/1‑93 (MQ=255)

                                                                     cagcaACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAAtctc                     <  2:4801432/93‑1 (MQ=255)

                                                                      agcaACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTcc                    <  2:3247064/93‑1 (MQ=255)

                                                                      agcaACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTcc                    >  2:4487455/1‑93 (MQ=255)

                                                                       gcaACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCg                   >  1:2956146/1‑93 (MQ=255)

                                                                       gcaACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCg                   <  1:1392963/93‑1 (MQ=255)

                                                                       gcaACATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGGCGCAACTTCGATAATCTCCg                   >  2:6044275/1‑93 (MQ=255)

                                                                        caACAGTCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCgg                  <  1:2003713/93‑1 (MQ=255)

                                                                         aaCATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCggg                 >  1:5590467/1‑93 (MQ=255)

                                                                         aaCATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCggg                 >  1:5347786/1‑93 (MQ=255)

                                                                         aaCATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCggg                 <  2:2396504/93‑1 (MQ=255)

                                                                          aCATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGc                >  1:3815128/1‑93 (MQ=255)

                                                                          aCATGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGc                <  1:4872495/93‑1 (MQ=255)

                                                                            aTGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCa              >  2:197661/1‑93 (MQ=255)

                                                                             tGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCaa             <  2:3737726/93‑1 (MQ=255)

                                                                             tGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCaa             >  1:1232218/1‑93 (MQ=255)

                                                                             tGCCGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCaa             >  1:6187391/1‑93 (MQ=255)

                                                                               ccGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAAc           >  2:865740/1‑93 (MQ=255)

                                                                               ccGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAAc           <  1:5723969/93‑1 (MQ=255)

                                                                                cGTTTTGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACa          <  1:4606684/93‑1 (MQ=255)

                                                                                  ttttGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACAtt        >  1:666601/1‑93 (MQ=255)

                                                                                  ttttGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACAtt        >  2:2693740/1‑93 (MQ=255)

                                                                                  ttttGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACAtt        >  2:284286/1‑93 (MQ=255)

                                                                                    ttGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTAc      >  1:531789/1‑93 (MQ=255)

                                                                                    ttGCCCGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTAc      <  2:3315968/93‑1 (MQ=255)

                                                                                       cccGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATg   <  1:2778030/93‑1 (MQ=255)

                                                                                       cccGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATg   <  2:1601974/93‑1 (MQ=255)

                                                                                       cccGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATg   <  1:4108031/93‑1 (MQ=255)

                                                                                       cccGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATg   >  2:3543874/1‑93 (MQ=255)

                                                                                        ccGTGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATGa  <  2:2162168/93‑1 (MQ=255)

ATACAGGCTGTTGATAGTGAAATCGCGGCGCTGGGCGTCTTCTTCGATGGAGCCGAAAATGTTGTCGCGCAGCAACATGCCGTTTTGCCCGCGTTGGGAGGTCGTGCGGTCGCTGACGTTACCTTCGTGGTGTCCACGGAAGGTCGCAACTTCGATAATCTCCGGGCCAAACATTACATGA  >  NC_000913/158620‑158800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`