BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,439,984	T→C	H295R (CAC→CGC)	ldhA ←	fermentative D‑lactate dehydrogenase, NAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,439,984	0	T	C	100.0%	838.8 / NA	256	H295R (CAC→CGC)	ldhA	fermentative D‑lactate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (121/135); total (121/135)

Only 100 of 263 total aligned reads displayed.

GGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAATAGA  >  NC_000913/1439895‑1440073

ggCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGcc                                                                                        <  1:6299385/93‑1 (MQ=255)

ggCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGcc                                                                                        >  1:792239/1‑93 (MQ=255)

 gCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGccc                                                                                       >  1:3200947/1‑93 (MQ=255)

 gCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGccc                                                                                       >  1:457318/1‑93 (MQ=255)

 gCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGccc                                                                                       >  2:3553110/1‑93 (MQ=255)

  cAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATTCTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCg                                                                                      <  2:5719477/93‑1 (MQ=255)

   aGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCgg                                                                                     >  2:2123534/1‑93 (MQ=255)

    ggTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAACTTCTGCTGTCAGGAATGCCTGGCGCCCGGt                                                                                    >  2:3442622/1‑93 (MQ=255)

     gTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTa                                                                                   <  2:3337201/93‑1 (MQ=255)

      tttCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTaa                                                                                  >  2:4495962/1‑93 (MQ=255)

       ttCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTaaa                                                                                 <  1:3402261/93‑1 (MQ=255)

        tCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAAc                                                                                <  1:4299880/93‑1 (MQ=255)

         cGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACa                                                                               <  1:588565/93‑1 (MQ=255)

         cGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACa                                                                               <  2:5999041/93‑1 (MQ=255)

          gCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAg                                                                              >  2:3721397/1‑93 (MQ=255)

          gCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAg                                                                              >  2:1805510/1‑93 (MQ=255)

           ccTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGc                                                                             >  1:2339278/1‑93 (MQ=255)

            cTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCa                                                                            >  1:1088525/1‑93 (MQ=255)

             tttttCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCAc                                                                           >  1:4073347/1‑93 (MQ=255)

             tttttCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCAc                                                                           >  1:4492127/1‑93 (MQ=255)

             tttttCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCAc                                                                           <  1:2043745/93‑1 (MQ=255)

                 tCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTtg                                                                       >  2:5883903/1‑93 (MQ=255)

                  ccAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTtgt                                                                      >  1:925165/1‑93 (MQ=255)

                  ccAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTtgt                                                                      >  1:1860712/1‑93 (MQ=255)

                   cAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCCGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTtgtg                                                                     <  2:4544331/93‑1 (MQ=255)

                    aGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTgg                                                                    <  2:4658364/93‑1 (MQ=255)

                    aGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTgg                                                                    <  2:2426828/93‑1 (MQ=255)

                     gATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGGCCGGTAAACAGCACGTTGTggc                                                                   >  2:5563131/1‑93 (MQ=255)

                     gATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTggc                                                                   >  1:2501993/1‑93 (MQ=255)

                     gATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTggc                                                                   >  1:3282964/1‑93 (MQ=255)

                     gATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTggc                                                                   <  1:2896360/93‑1 (MQ=255)

                     gATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTggc                                                                   >  1:5560290/1‑93 (MQ=255)

                     gATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTCCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTggc                                                                   >  2:5378184/1‑93 (MQ=255)

                     gATTGCTTAAGTTTTGCAGCGCAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAAAGCCTGGCGCCCGGTAAACAGCACGTTGTggc                                                                   >  2:1834412/1‑93 (MQ=255)

                      aTTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTggca                                                                  >  1:470840/1‑93 (MQ=255)

                       ttGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTggcag                                                                 >  1:4091419/1‑93 (MQ=255)

                         gCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAGACAGCACGTTGTggcaggc                                                               <  2:6145919/93‑1 (MQ=255)

                          cTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTggcaggga                                                              >  2:2287710/1‑91 (MQ=255)

                           ttAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGgcaggcag                                                             <  2:5231103/93‑1 (MQ=255)

                            tAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGa                                                            >  2:3714194/1‑93 (MQ=255)

                             aaGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGAc                                                           <  2:6289179/93‑1 (MQ=255)

                               gTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAg                                                         >  2:3032339/1‑93 (MQ=255)

                                ttttGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAgg                                                        <  1:1142807/93‑1 (MQ=255)

                                ttttGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAgg                                                        <  1:3049069/93‑1 (MQ=255)

                                   tGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGa                                                     <  1:3819736/93‑1 (MQ=255)

                                    gCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGAc                                                    <  2:4265449/93‑1 (MQ=255)

                                      aGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGGAGACAGGCGACgg                                                  >  1:5051191/1‑93 (MQ=255)

                                      aGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACgg                                                  >  2:2738607/1‑93 (MQ=255)

                                       gCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGa                                                 >  2:1309291/1‑93 (MQ=255)

                                        cGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGaa                                                >  2:5508275/1‑93 (MQ=255)

                                          tAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCCGACAGGCGGCGGAATa                                              >  2:1596693/1‑93 (MQ=255)

                                           aGTCTGAGAAGTACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATAc                                             <  1:1477775/93‑1 (MQ=255)

                                           aGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATAc                                             <  1:3671829/93‑1 (MQ=255)

                                           aGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATAc                                             <  1:1377569/93‑1 (MQ=255)

                                              cTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTc                                          <  1:6220895/93‑1 (MQ=255)

                                               tGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGGATACGTCa                                         >  1:156533/1‑93 (MQ=255)

                                               tGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCa                                         >  2:1427489/1‑93 (MQ=255)

                                               tGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCa                                         <  2:1497490/93‑1 (MQ=255)

                                               tGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCa                                         >  2:2399014/1‑93 (MQ=255)

                                                gagaAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGCATACGTCAt                                        >  2:81259/1‑93 (MQ=255)

                                                 agaAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATc                                       <  2:3971132/93‑1 (MQ=255)

                                                   aaaTACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCt                                     <  1:5805576/93‑1 (MQ=255)

                                                    aaTACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTg                                    <  2:4460034/93‑1 (MQ=255)

                                                    aaTACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTg                                    >  2:3470139/1‑93 (MQ=255)

                                                     aTACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTgg                                   <  2:1946783/93‑1 (MQ=255)

                                                      tACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGa                                  <  2:5575501/93‑1 (MQ=255)

                                                      tACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGa                                  >  1:5817547/1‑93 (MQ=255)

                                                      tACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGa                                  >  1:1642996/1‑93 (MQ=255)

                                                       aCTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGGCGACAGGCGACGGAATACGTCATCCTGGAt                                 >  1:2609214/1‑93 (MQ=255)

                                                         tGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCa                               <  1:2445084/93‑1 (MQ=255)

                                                         tGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCa                               <  2:2817653/93‑1 (MQ=255)

                                                          ggTCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCAc                              <  2:4131242/93‑1 (MQ=255)

                                                            tCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCGTCCTGGATCAcgt                            >  1:3604858/1‑93 (MQ=255)

                                                            tCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCAcgc                            >  2:877705/1‑92 (MQ=255)

                                                            tCAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCAcct                            >  2:3842791/1‑91 (MQ=255)

                                                            gcAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCAcgt                            <  1:5109997/92‑1 (MQ=255)

                                                             cAGAGCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGGAGACAGGCGACGGAATAAGTCATCCTGGATCAcgtc                           >  1:5283717/1‑93 (MQ=255)

                                                              agagCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCAcgtcg                          >  2:931927/1‑93 (MQ=255)

                                                               gagCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGGAGACAGGCGACGGAATACGACATCCTGGATCAcgccgt                         >  2:5816574/1‑93 (MQ=255)

                                                                agCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGTTATGCAAACAGAAGACGGCATACGTCATCCTGGATCACGTCGtt                        <  1:1057771/93‑1 (MQ=255)

                                                                 gCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGGATACGTCATCCTGGATCACGGCGTTg                       >  2:1146042/1‑93 (MQ=255)

                                                                 gCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTg                       <  1:5637239/93‑1 (MQ=255)

                                                                 gCTTCTGCTGTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATACTGGATCACGTCGTTg                       >  1:1819913/1‑93 (MQ=255)

                                                                      tgctgTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTTGTTGGGttt                  >  1:950466/1‑93 (MQ=255)

                                                                        ctgTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTAt                <  1:3230676/93‑1 (MQ=255)

                                                                          gTCAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCt              >  1:4334058/1‑93 (MQ=255)

                                                                            cAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTc            <  1:1697443/93‑1 (MQ=255)

                                                                            cAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTc            <  1:3903530/93‑1 (MQ=255)

                                                                            cAGGAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTc            <  1:5204711/93‑1 (MQ=255)

                                                                              ggAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCaa          <  1:3716361/93‑1 (MQ=255)

                                                                              ggAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCaa          <  1:5870563/93‑1 (MQ=255)

                                                                              ggAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCaa          <  2:2946808/93‑1 (MQ=255)

                                                                               gAATGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTTaaa         >  2:1109455/1‑93 (MQ=255)

                                                                                aaTGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAg        <  1:4774537/93‑1 (MQ=255)

                                                                                 aagCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGAAAGGCGACGGAATACGGCATCCTGGATCACGTCGTTGGATTTATCTTCcaaaa       >  2:3295897/3‑91 (MQ=255)

                                                                                 aTGCCTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGa       >  1:5467200/1‑93 (MQ=255)

                                                                                    ccTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAATa    <  2:4963558/93‑1 (MQ=255)

                                                                                     cTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAATAg   >  1:4986440/1‑93 (MQ=255)

                                                                                     cTGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAATAg   >  1:2013154/1‑93 (MQ=255)

                                                                                      tGGCGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAATAGa  <  2:1002844/93‑1 (MQ=255)

GGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAATAGA  >  NC_000913/1439895‑1440073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`