BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	16,925	A→G	R12R (CGT→CGC)	gef ←	Gef

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	16,925	0	A	G	99.5%	604.3 / NA	185	R12R (CGT→CGC)	gef	Gef
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (57/127); minor base C (0/1); total (57/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

CAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCT  >  NC_000913/16835‑17012

cAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCg                                                                                        <  1:5391694/93‑1 (MQ=255)

cAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCg                                                                                        >  1:5020724/1‑93 (MQ=255)

cAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCg                                                                                        <  2:3089510/93‑1 (MQ=255)

 aGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCgg                                                                                       >  2:1726293/1‑93 (MQ=255)

  gcgcCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGt                                                                                      <  2:5678737/93‑1 (MQ=255)

    cccgccACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCa                                                                                    <  2:4036307/92‑1 (MQ=255)

      cgccACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGt                                                                                  <  2:3532635/93‑1 (MQ=255)

      cgccACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGt                                                                                  >  2:5696755/1‑93 (MQ=255)

      cgccACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGt                                                                                  >  1:2384103/1‑93 (MQ=255)

       gccACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTa                                                                                 <  2:5693957/93‑1 (MQ=255)

           cTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAagag                                                                             >  1:459733/1‑93 (MQ=255)

            tACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAgg                                                                            >  1:880388/1‑93 (MQ=255)

             aCGGCGGTGATACAGATGACGATCAGGGCGACAATCCTCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGc                                                                           >  2:1764877/1‑93 (MQ=255)

             aCGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGc                                                                           >  1:2556816/1‑93 (MQ=255)

             aCGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGc                                                                           >  1:2729957/1‑93 (MQ=255)

             aCGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGc                                                                           <  1:3025873/93‑1 (MQ=255)

             aCGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGc                                                                           <  2:5798747/93‑1 (MQ=255)

              cggcggTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCa                                                                          <  2:5226586/93‑1 (MQ=255)

              cggcggTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCa                                                                          <  1:3316999/93‑1 (MQ=255)

              cggcggTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCa                                                                          <  2:538528/93‑1 (MQ=255)

                gcggTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCAct                                                                        >  1:2607554/1‑93 (MQ=255)

                gcggTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCAct                                                                        >  2:145046/1‑93 (MQ=255)

                gcggTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCAct                                                                        <  1:449983/93‑1 (MQ=255)

                  ggTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCActct                                                                      >  1:5554310/1‑93 (MQ=255)

                   gTGATGCAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTa                                                                     <  1:4908245/93‑1 (MQ=255)

                   gTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTa                                                                     >  2:4385416/1‑93 (MQ=255)

                    tGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTAc                                                                    <  1:3659387/93‑1 (MQ=255)

                     gATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACa                                                                   <  2:3309691/93‑1 (MQ=255)

                      aTACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACAt                                                                  >  1:2060527/1‑93 (MQ=255)

                       tACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACAtg                                                                 >  2:4785988/1‑93 (MQ=255)

                        aCAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACAtgt                                                                <  2:4538039/93‑1 (MQ=255)

                         cAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACAtgtg                                                               <  1:4367169/93‑1 (MQ=255)

                            aTGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTc                                                            <  1:639474/93‑1 (MQ=255)

                             tGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCa                                                           >  1:2173166/1‑93 (MQ=255)

                             tGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCa                                                           <  1:98967/93‑1 (MQ=255)

                             tGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCa                                                           <  2:673844/93‑1 (MQ=255)

                               aCGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGc                                                         <  2:1096782/93‑1 (MQ=255)

                                cGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCa                                                        <  1:5135965/93‑1 (MQ=255)

                                 gATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCat                                                       <  1:5959404/93‑1 (MQ=255)

                                 gATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCat                                                       <  2:2397831/93‑1 (MQ=255)

                                   tCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCatat                                                     >  2:3181293/1‑93 (MQ=255)

                                   tCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCatat                                                     >  1:4937005/1‑93 (MQ=255)

                                    cAGGGCGACAATCATCGCCTTATTCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCAtata                                                    <  2:5447459/93‑1 (MQ=255)

                                      gggCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATagg                                                  <  2:1290630/93‑1 (MQ=255)

                                      gggCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATagg                                                  >  1:4150220/1‑93 (MQ=255)

                                       ggCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATagga                                                 <  1:5657166/93‑1 (MQ=255)

                                        gCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATaggag                                                <  1:1614308/93‑1 (MQ=255)

                                        gCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATaggag                                                <  1:1814686/93‑1 (MQ=255)

                                        gCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATaggag                                                <  2:4512521/93‑1 (MQ=255)

                                          gACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGc                                              <  1:1048436/93‑1 (MQ=255)

                                          gACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGc                                              >  2:4178522/1‑93 (MQ=255)

                                           aCAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGcc                                             <  1:534715/93‑1 (MQ=255)

                                           aCAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGcc                                             >  2:4515595/1‑93 (MQ=255)

                                           aCAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGcc                                             <  2:4628303/93‑1 (MQ=255)

                                             aaTCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑Tc                                          <  2:5956863/93‑1 (MQ=255)

                                              atcatcGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCg                                         >  1:3261209/1‑93 (MQ=255)

                                              atcatcGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCg                                         <  2:1876214/93‑1 (MQ=255)

                                              atcatcGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCg                                         <  2:3337896/93‑1 (MQ=255)

                                               tcatcGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCgg                                        <  1:4661697/93‑1 (MQ=255)

                                                catcGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCggg                                       >  1:4685057/1‑93 (MQ=255)

                                                 atcGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGt                                      <  2:5500073/93‑1 (MQ=255)

                                                 atcGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGt                                      <  2:63359/93‑1 (MQ=255)

                                                  tcGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGtt                                     >  2:4435163/1‑93 (MQ=255)

                                                  tcGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGtt                                     <  1:543418/93‑1 (MQ=255)

                                                    gCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGa                                   <  2:3593980/93‑1 (MQ=255)

                                                    gCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGa                                   <  1:1084123/93‑1 (MQ=255)

                                                    gCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGa                                   <  2:229630/93‑1 (MQ=255)

                                                    gCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGa                                   <  1:4830565/93‑1 (MQ=255)

                                                      cTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATg                                 <  2:3724522/93‑1 (MQ=255)

                                                       ttATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATgg                                <  2:386810/93‑1 (MQ=255)

                                                       ctATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATgg                                <  1:1289912/92‑1 (MQ=255)

                                                         aTGCTGCTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTa                              >  2:1418615/1‑93 (MQ=255)

                                                          tgctgcTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTaa                             <  1:6222497/93‑1 (MQ=255)

                                                          tgctgcTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTaa                             >  1:3216688/1‑93 (MQ=255)

                                                          tgctgcTTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTaa                             >  1:4622329/1‑93 (MQ=255)

                                                           gctgctTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTaaa                            >  2:4377685/1‑93 (MQ=255)

                                                            ctgctTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTaaaa                           <  1:232264/93‑1 (MQ=255)

                                                             tgctTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAat                          <  2:1563741/93‑1 (MQ=255)

                                                             tgctTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATA‑GAGGCCTTGGGGTTGTTGGaaaatt                          >  1:5259752/1‑91 (MQ=255)

                                                               ctTCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAatat                        <  2:1204735/93‑1 (MQ=255)

                                                                 tCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCa                      <  2:3900983/93‑1 (MQ=255)

                                                                 tCATTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCa                      <  1:6125157/93‑1 (MQ=255)

                                                                   aTTGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACt                    <  1:5206889/93‑1 (MQ=255)

                                                                    ttGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTc                   <  1:1234015/93‑1 (MQ=255)

                                                                    ttGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTc                   <  2:4389191/93‑1 (MQ=255)

                                                                     tGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCg                  <  1:4624258/93‑1 (MQ=255)

                                                                     tGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCg                  <  1:4203166/93‑1 (MQ=255)

                                                                     tGCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCg                  <  1:325483/93‑1 (MQ=255)

                                                                      gCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCgg                 >  2:4840327/1‑93 (MQ=255)

                                                                      gCTCTCTTCTCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCgg                 >  1:3915824/1‑93 (MQ=255)

                                                                          tcttctCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCt             <  2:4931542/93‑1 (MQ=255)

                                                                            ttctCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCttt           >  2:2752667/1‑93 (MQ=255)

                                                                            ttctCCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCttt           <  2:6191343/93‑1 (MQ=255)

                                                                              ctcCTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCTTTtc         <  2:3423035/93‑1 (MQ=255)

                                                                                ccTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCTTTtctc       <  2:5020724/93‑1 (MQ=255)

                                                                                 cTTGACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCTTTTctct      <  2:2361627/93‑1 (MQ=255)

                                                                                    gACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATc   >  2:2688619/1‑93 (MQ=255)

                                                                                    gACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATc   <  2:6283171/93‑1 (MQ=255)

                                                                                    gACCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATc   <  2:2973307/93‑1 (MQ=255)

                                                                                     aCCTTGCGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCt  <  2:2233597/93‑1 (MQ=255)

CAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCC‑TCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCT  >  NC_000913/16835‑17012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`