BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,477,747	A→G	I196V (ATT→GTT)	b1410 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,477,747	0	A	G	99.4%	575.0 / NA	179	I196V (ATT→GTT)	b1410	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (94/84); minor base T (0/1); total (94/85)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

TTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTG  >  NC_000913/1477657‑1477836

tttATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGtt                                                                                         >  1:3167422/1‑93 (MQ=255)

    ttaATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCg                                                                                     >  2:1309502/1‑93 (MQ=255)

     taATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGt                                                                                    >  2:4246729/1‑93 (MQ=255)

       aTTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTa                                                                                  >  2:176262/1‑93 (MQ=255)

       aTTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTa                                                                                  <  2:1400132/93‑1 (MQ=255)

         tCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAat                                                                                >  2:6060660/1‑93 (MQ=255)

          ccTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAata                                                                               <  2:1993336/93‑1 (MQ=255)

           cTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAatat                                                                              >  1:4075486/1‑93 (MQ=255)

            tATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATc                                                                             >  1:2417901/1‑93 (MQ=255)

            tATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATc                                                                             >  2:4257343/1‑93 (MQ=255)

             aTGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCt                                                                            <  2:3775240/93‑1 (MQ=255)

              tgtgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCtt                                                                           >  1:1759536/1‑93 (MQ=255)

              tgtgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCtt                                                                           <  1:1069182/93‑1 (MQ=255)

               gtgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTg                                                                          <  2:6044379/93‑1 (MQ=255)

                tgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGc                                                                         <  2:4114426/93‑1 (MQ=255)

                tgAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGc                                                                         <  2:5970377/93‑1 (MQ=255)

                 gAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCt                                                                        >  1:1234488/1‑93 (MQ=255)

                 gAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCt                                                                        >  2:1540176/1‑93 (MQ=255)

                  aaaGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTc                                                                       >  1:2206141/1‑93 (MQ=255)

                   aaGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCg                                                                      >  1:6294839/1‑93 (MQ=255)

                    aGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGa                                                                     >  1:1767257/1‑93 (MQ=255)

                    aGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGa                                                                     >  2:317560/1‑93 (MQ=255)

                    aGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGCGGTTTGCGGTT‑ATATCTTTCttggt                                                                    >  1:1267090/1‑89 (MQ=255)

                     ggACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAt                                                                    <  2:754681/93‑1 (MQ=255)

                      gACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtc                                                                   <  2:2279122/93‑1 (MQ=255)

                      gACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtc                                                                   >  1:3325839/1‑93 (MQ=255)

                      gACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtc                                                                   <  1:5168728/93‑1 (MQ=255)

                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 >  2:2841162/1‑93 (MQ=255)

                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 <  2:4538363/93‑1 (MQ=255)

                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 >  2:5633345/1‑93 (MQ=255)

                        cGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGAtctc                                                                 <  1:2681663/93‑1 (MQ=255)

                        cGCTATTTGACCCACGATCGGCAAC‑GGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATctct                                                                >  1:3719100/1‑93 (MQ=255)

                         gCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATctct                                                                >  1:3585618/1‑93 (MQ=255)

                           tATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTAc                                                              >  1:4287747/1‑93 (MQ=255)

                             tttGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaa                                                            >  2:3922449/1‑93 (MQ=255)

                              ttGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaaa                                                           >  1:3359706/1‑93 (MQ=255)

                              ttGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaaa                                                           >  1:2276732/1‑93 (MQ=255)

                               tGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACaaaa                                                          <  1:2130633/93‑1 (MQ=255)

                                  cccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGt                                                       <  1:4530159/93‑1 (MQ=255)

                                   ccACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTc                                                      >  2:6224029/1‑93 (MQ=255)

                                    cACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCt                                                     <  1:845257/93‑1 (MQ=255)

                                     aCGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTg                                                    >  1:3178303/1‑93 (MQ=255)

                                      cGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTAGATCTCTACAAAACGTCTGa                                                   <  2:1069882/93‑1 (MQ=255)

                                       gATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGaa                                                  <  1:421447/93‑1 (MQ=255)

                                       gATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGaa                                                  <  1:1786223/93‑1 (MQ=255)

                                        aTCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAAc                                                 >  1:5731266/1‑93 (MQ=255)

                                         tCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                                <  1:3726710/93‑1 (MQ=255)

                                         tCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACg                                                >  2:2863249/1‑93 (MQ=255)

                                          cGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGt                                               <  1:1363880/93‑1 (MQ=255)

                                           ggCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGta                                              >  2:2074081/1‑93 (MQ=255)

                                            gCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtat                                             >  1:4884901/1‑93 (MQ=255)

                                            gCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtat                                             <  2:193914/93‑1 (MQ=255)

                                            gCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtat                                             <  2:2145557/93‑1 (MQ=255)

                                             cAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtatt                                            <  2:3385461/93‑1 (MQ=255)

                                              aaCGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGtatta                                           >  2:5159713/1‑93 (MQ=255)

                                                 ggggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAttatta                                        <  2:4179078/93‑1 (MQ=255)

                                                 ggggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAttatta                                        <  2:1841282/93‑1 (MQ=255)

                                                 ggggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAttatta                                        >  2:2855062/1‑93 (MQ=255)

                                                 ggggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTAttatta                                        <  2:858721/93‑1 (MQ=255)

                                                  gggggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTag                                       <  1:4612535/93‑1 (MQ=255)

                                                    gggCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTagag                                     >  2:3045448/1‑93 (MQ=255)

                                                      gCGAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGAt                                   >  2:4867546/1‑93 (MQ=255)

                                                        gAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgc                                 <  2:2684365/93‑1 (MQ=255)

                                                        gAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgc                                 >  2:3508752/1‑93 (MQ=255)

                                                        gAGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgc                                 >  2:521330/1‑93 (MQ=255)

                                                        gAGTTTCAATAATGACCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgc                                 <  2:6001052/93‑1 (MQ=255)

                                                         aGTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcg                                <  2:4987282/93‑1 (MQ=255)

                                                          gTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcgg                               >  2:5264952/1‑93 (MQ=255)

                                                          gTTTCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcgg                               <  1:5593062/93‑1 (MQ=255)

                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggc                              >  2:5537304/1‑93 (MQ=255)

                                                           tttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAAAGTCTGAACGAATTATTAGAGATgcggc                              >  2:2035933/1‑93 (MQ=255)

                                                            ttCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcg                             <  1:3105851/93‑1 (MQ=255)

                                                             tCAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcgg                            >  2:3257679/1‑93 (MQ=255)

                                                              cAATAATGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTTCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcggc                           <  2:63023/93‑1 (MQ=255)

                                                               aataatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATgcggcggcg                          >  1:110072/1‑93 (MQ=255)

                                                                ataatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGa                         >  1:3999763/1‑93 (MQ=255)

                                                                ataatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGa                         >  2:649289/1‑93 (MQ=255)

                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        <  1:189709/93‑1 (MQ=255)

                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        >  2:242689/1‑93 (MQ=255)

                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        >  2:2221104/1‑93 (MQ=255)

                                                                 taatGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGAt                        <  2:3441199/93‑1 (MQ=255)

                                                                   atGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTa                      >  1:6101835/1‑93 (MQ=255)

                                                                    tGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAc                     <  1:349385/93‑1 (MQ=255)

                                                                    tGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAc                     <  2:4749267/93‑1 (MQ=255)

                                                                    tGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAc                     >  1:2903508/1‑93 (MQ=255)

                                                                    tGATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTAc                     >  1:4492068/1‑93 (MQ=255)

                                                                     gATCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACg                    >  1:592821/1‑93 (MQ=255)

                                                                      aTCCGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGc                   <  2:5449491/93‑1 (MQ=255)

                                                                        ccGCTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTc                 >  1:3433097/1‑93 (MQ=255)

                                                                           cTGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTcccc              <  2:5576819/93‑1 (MQ=255)

                                                                            tGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCa             >  2:4972478/1‑93 (MQ=255)

                                                                            tGATCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCa             <  1:1475509/93‑1 (MQ=255)

                                                                               tCACACGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGc          >  1:2106291/1‑93 (MQ=255)

                                                                                cacaCGGGCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCa         >  1:5482770/1‑93 (MQ=255)

                                                                                     gggCGTTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTc    <  2:2765440/93‑1 (MQ=255)

                                                                                     gggCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTc    <  2:4796637/93‑1 (MQ=255)

                                                                                     gggCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATGACGCTCCCCACGCAACTTc    <  2:6137469/93‑1 (MQ=255)

                                                                                      ggCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCt   <  1:900949/93‑1 (MQ=255)

                                                                                      ggCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCt   >  2:1637769/1‑93 (MQ=255)

                                                                                       gCGGTTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTg  >  2:2933777/1‑93 (MQ=255)

TTTATTAATTCCTATGTGAAAGGACGCTATTTGACCCACGATCGGCAACGGGGGGCGAGTTTCAATAATGATCCGCTGATCACACGGGCGATTGCCGTTAATATCTTGCTCGATCTCTACAAAACGTCTGAACGTATTATTAGAGATGCGGCGGCGATTACGCTCCCCACGCAACTTCTG  >  NC_000913/1477657‑1477836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`