BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,570,594	G→A	A878V (GCG→GTG)	pqqL ←	putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,570,594	0	G	A	99.6%	862.8 / NA	232	A878V (GCG→GTG)	pqqL	putative peptidase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (144/87); minor base T (0/1); total (144/88)
Fisher's exact test for biased strand distribution p-value = 3.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

TTCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCGCTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGATCCCTTTA  >  NC_000913/1570504‑1570683

ttCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACt                                                                                         >  1:1009345/1‑93 (MQ=255)

ttCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACt                                                                                         >  1:1720483/1‑93 (MQ=255)

ttCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACt                                                                                         >  2:1457966/1‑93 (MQ=255)

    ctACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAAtt                                                                                     <  2:2101997/93‑1 (MQ=255)

     tACCGTCATTTGTTTCAACGATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAAttg                                                                                    >  2:6081417/1‑93 (MQ=255)

      aCCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAAttgt                                                                                   >  2:6279214/1‑93 (MQ=255)

        cGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAAttgttg                                                                                 >  1:6129298/1‑93 (MQ=255)

        cGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAAttgttg                                                                                 >  1:3560775/1‑93 (MQ=255)

        cGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAAttgttg                                                                                 >  1:2924969/1‑93 (MQ=255)

          tCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGaa                                                                               <  2:728447/93‑1 (MQ=255)

          tCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGaa                                                                               >  2:1015267/1‑93 (MQ=255)

           cATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAac                                                                              >  2:3852865/1‑93 (MQ=255)

             tttGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAacgc                                                                            >  2:4342902/1‑93 (MQ=255)

             tttGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAacgc                                                                            >  1:2978166/1‑93 (MQ=255)

              ttGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAacgct                                                                           >  1:2141700/1‑93 (MQ=255)

               tGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAacgcta                                                                          >  2:3471906/1‑93 (MQ=255)

               tGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAacgcta                                                                          <  1:895157/93‑1 (MQ=255)

               tGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAacgcta                                                                          >  1:3852378/1‑93 (MQ=255)

                gTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAacgctac                                                                         >  1:2044140/1‑93 (MQ=255)

                  ttCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAacgctacgc                                                                       >  1:4935659/1‑93 (MQ=255)

                      acaaTTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGtt                                                                   <  1:6306121/93‑1 (MQ=255)

                      acaaTTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGtt                                                                   >  1:1897832/1‑93 (MQ=255)

                      acaaTTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGtt                                                                   >  2:2651100/1‑93 (MQ=255)

                       caaTTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTg                                                                  <  1:4787723/93‑1 (MQ=255)

                        aaTTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTgg                                                                 >  1:5431624/1‑93 (MQ=255)

                         aTTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGa                                                                >  1:43316/1‑93 (MQ=255)

                         aTTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGa                                                                >  2:1127978/1‑93 (MQ=255)

                         aTTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGa                                                                <  2:4759185/93‑1 (MQ=255)

                          ttGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGat                                                               >  1:4534207/1‑93 (MQ=255)

                             cTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATc                                                            >  1:3121587/1‑93 (MQ=255)

                             cTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATc                                                            <  1:13839/93‑1 (MQ=255)

                              tCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCg                                                           >  1:3454473/1‑93 (MQ=255)

                              tCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCg                                                           >  2:4825523/1‑93 (MQ=255)

                               ccTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGa                                                          >  1:392492/1‑93 (MQ=255)

                                cTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAg                                                         >  1:40639/1‑93 (MQ=255)

                                 tGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAgg                                                        >  2:3025633/1‑93 (MQ=255)

                                 tGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAgg                                                        >  2:869226/1‑93 (MQ=255)

                                  gCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGc                                                       >  1:1657102/1‑93 (MQ=255)

                                   cTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCt                                                      >  2:5664945/1‑93 (MQ=255)

                                    tCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTg                                                     <  2:1383059/93‑1 (MQ=255)

                                    tCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTg                                                     >  1:1297720/1‑93 (MQ=255)

                                    tCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTg                                                     >  1:6075941/1‑93 (MQ=255)

                                      aGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTgcg                                                   >  1:5264689/1‑93 (MQ=255)

                                      aGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTgcg                                                   <  2:1093449/93‑1 (MQ=255)

                                        tCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCt                                                 <  2:5375173/93‑1 (MQ=255)

                                          cATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGa                                               >  2:4395573/1‑93 (MQ=255)

                                           aTGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGaa                                              >  2:1740733/1‑93 (MQ=255)

                                            tgctgcAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAAc                                             <  1:3035516/93‑1 (MQ=255)

                                               tgcAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGtt                                          <  1:6249181/93‑1 (MQ=255)

                                                gcAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGttt                                         <  1:504851/93‑1 (MQ=255)

                                                gcAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGttt                                         >  2:3752614/1‑93 (MQ=255)

                                                gcAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGttt                                         >  1:552455/1‑93 (MQ=255)

                                                   ggATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGc                                      <  1:2487561/93‑1 (MQ=255)

                                                   ggATCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGc                                      >  2:3879885/1‑93 (MQ=255)

                                                     aTCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTg                                    >  2:3560019/1‑93 (MQ=255)

                                                      tCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTgg                                   <  1:1595416/93‑1 (MQ=255)

                                                      tCGTCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTgg                                   <  1:4375478/93‑1 (MQ=255)

                                                         tCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTat                                >  2:5095481/1‑93 (MQ=255)

                                                         tCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTat                                >  2:4933732/1‑93 (MQ=255)

                                                         tCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTat                                >  2:118538/1‑93 (MQ=255)

                                                         tCATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTCGATATCGAGGCTGCGCTGAACGTTTTGCTGGTat                                >  2:3980207/1‑93 (MQ=255)

                                                          cATATTGAATAAGACTATTTACCATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCTCTGAACGGTTTGCTGGGatt                               >  2:5781764/1‑93 (MQ=255)

                                                          cATATTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTatt                               >  2:311488/1‑93 (MQ=255)

                                                             atTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTattcat                            <  2:5819121/93‑1 (MQ=255)

                                                             atTGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTattcat                            <  2:243006/93‑1 (MQ=255)

                                                              ttGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTattcatt                           >  2:2867464/1‑93 (MQ=255)

                                                               tGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTattcattc                          <  1:5052394/93‑1 (MQ=255)

                                                               tGAATAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTattcattc                          <  2:3328396/93‑1 (MQ=255)

                                                                 aaTAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAg                        <  2:4112662/93‑1 (MQ=255)

                                                                 aaTAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAg                        >  2:5200110/1‑93 (MQ=255)

                                                                  aTAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGt                       >  1:2294483/1‑93 (MQ=255)

                                                                  aTAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGt                       <  1:4220292/93‑1 (MQ=255)

                                                                  aTAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGt                       >  1:5759579/1‑93 (MQ=255)

                                                                  aTAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGt                       >  1:4518200/1‑93 (MQ=255)

                                                                  aTAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGt                       >  2:5716867/1‑93 (MQ=255)

                                                                   tAAGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGtt                      >  2:3230573/1‑93 (MQ=255)

                                                                    aaGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTc                     >  2:4804128/1‑93 (MQ=255)

                                                                    aaGACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTc                     >  1:1087052/1‑93 (MQ=255)

                                                                      gACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCtt                   >  1:5741458/1‑93 (MQ=255)

                                                                      gACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCtt                   <  1:4113463/93‑1 (MQ=255)

                                                                      gACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCtt                   >  1:747112/1‑93 (MQ=255)

                                                                      gACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCtt                   >  2:5315576/1‑93 (MQ=255)

                                                                      gACTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCtt                   >  1:4449654/1‑93 (MQ=255)

                                                                       aCTATTTACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTg                  >  2:4305401/1‑93 (MQ=255)

                                                                           tttACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCa              <  1:3319153/93‑1 (MQ=255)

                                                                             tACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACt            <  2:4029047/93‑1 (MQ=255)

                                                                             tACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACt            <  2:2739202/93‑1 (MQ=255)

                                                                             tACAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACt            <  1:829782/93‑1 (MQ=255)

                                                                              aCAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTg           >  2:3619721/1‑93 (MQ=255)

                                                                               cAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGa          >  1:5147123/1‑93 (MQ=255)

                                                                               cAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGa          <  2:5977449/93‑1 (MQ=255)

                                                                               cAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGa          <  2:1873323/93‑1 (MQ=255)

                                                                               cAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGa          <  2:1926416/93‑1 (MQ=255)

                                                                               cAATAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGa          <  1:3829517/93‑1 (MQ=255)

                                                                                aaTAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGAt         >  1:5672535/1‑93 (MQ=255)

                                                                                 aTAGTGTTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGACCGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGATc        <  2:5877734/93‑1 (MQ=255)

                                                                                    gtgtTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGATCGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGATCCCt     <  1:705907/93‑1 (MQ=255)

                                                                                    gtgtTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGATCCCt     >  1:4753401/1‑93 (MQ=255)

                                                                                     tgtTCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGATCCCtt    <  2:1555614/93‑1 (MQ=255)

                                                                                       ttCACTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGATCCCTTTa  <  1:2580495/93‑1 (MQ=255)

TTCTCTACCGTCATTTGTTTCAACAATTGCTCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCGCTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCATTCAGTTCTTGCTCACTGATCCCTTTA  >  NC_000913/1570504‑1570683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`