BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,594,520	A→G	S531P (TCC→CCC)	ydeK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,594,520	0	A	G	99.5%	613.4 / NA	191	S531P (TCC→CCC)	ydeK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (87/102); total (88/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 197 total aligned reads displayed.

ATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACA  >  NC_000913/1594430‑1594608

aTACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGgggg                                                                                        >  2:4334980/1‑93 (MQ=255)

aTACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGgggg                                                                                        <  1:2728656/93‑1 (MQ=255)

 tACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGc                                                                                       >  1:1522956/1‑93 (MQ=255)

    acCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCAtt                                                                                    >  1:4926777/1‑93 (MQ=255)

    acCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCAtt                                                                                    <  2:4797709/93‑1 (MQ=255)

    acCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCAtt                                                                                    >  2:1544586/1‑93 (MQ=255)

     ccGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCAttt                                                                                   <  1:1158281/93‑1 (MQ=255)

     ccGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCAttt                                                                                   <  1:6294254/93‑1 (MQ=255)

      cGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTtc                                                                                  <  1:6072202/93‑1 (MQ=255)

       gACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTtcc                                                                                 >  1:5362626/1‑93 (MQ=255)

        aCCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTtcct                                                                                >  2:2246506/1‑93 (MQ=255)

        aCCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTtcct                                                                                >  2:296082/1‑93 (MQ=255)

          cTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTtcctcc                                                                              <  1:1680175/93‑1 (MQ=255)

           tGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCg                                                                             >  2:382381/1‑93 (MQ=255)

             tAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGAt                                                                           <  2:1842296/93‑1 (MQ=255)

             tAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGAt                                                                           <  2:6061023/93‑1 (MQ=255)

             tAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGAt                                                                           <  2:2609021/93‑1 (MQ=255)

              aGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATa                                                                          >  1:6013346/1‑93 (MQ=255)

               gCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACAGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATaa                                                                         >  1:3383220/1‑93 (MQ=255)

                 aaTTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAAt                                                                       <  1:445618/93‑1 (MQ=255)

                  aTTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATc                                                                      >  2:2804508/1‑93 (MQ=255)

                  aTTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATc                                                                      <  1:5236684/93‑1 (MQ=255)

                  aTTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATc                                                                      >  1:2062393/1‑93 (MQ=255)

                   ttGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATcc                                                                     <  2:685802/93‑1 (MQ=255)

                   ttGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATcc                                                                     >  2:3986075/1‑93 (MQ=255)

                    tgtgCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCa                                                                    <  1:5031989/93‑1 (MQ=255)

                     gtgCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCAt                                                                   <  1:776128/93‑1 (MQ=255)

                     gtgCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCAt                                                                   <  2:1100769/93‑1 (MQ=255)

                      tgCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATa                                                                  >  1:5841937/1‑93 (MQ=255)

                       gCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAg                                                                 <  2:5851721/93‑1 (MQ=255)

                         gTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGcc                                                               >  1:2146425/1‑93 (MQ=255)

                          ttAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGccc                                                              <  2:493770/93‑1 (MQ=255)

                          ttAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGccc                                                              >  2:2369630/1‑93 (MQ=255)

                           tagtagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCg                                                             >  1:2903793/1‑93 (MQ=255)

                           tagtagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCg                                                             <  1:1802955/93‑1 (MQ=255)

                           tagtagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCg                                                             <  1:3873506/93‑1 (MQ=255)

                             gtagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGtt                                                           <  2:1373062/93‑1 (MQ=255)

                                gatgatGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGaa                                                        >  2:5439506/1‑93 (MQ=255)

                                gatgatGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGaa                                                        >  2:4269351/1‑93 (MQ=255)

                                gatgatGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGaa                                                        >  2:1670533/1‑93 (MQ=255)

                                  tgatgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAtt                                                      <  1:2080903/93‑1 (MQ=255)

                                  tgatgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAtt                                                      <  1:1927620/93‑1 (MQ=255)

                                   gatgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTa                                                     >  1:3674411/1‑93 (MQ=255)

                                   gatgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTa                                                     >  1:6243666/1‑93 (MQ=255)

                                    atgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTaa                                                    <  2:5742829/93‑1 (MQ=255)

                                    atgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTaa                                                    >  2:6228342/1‑93 (MQ=255)

                                     tgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAg                                                   <  1:5743106/93‑1 (MQ=255)

                                     tgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAg                                                   <  1:2256160/93‑1 (MQ=255)

                                      ttCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAgg                                                  <  1:1248610/92‑1 (MQ=255)

                                      gTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCACCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAgg                                                  <  1:1544044/93‑1 (MQ=255)

                                       tCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAggg                                                 <  2:3940414/93‑1 (MQ=255)

                                         tttAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAgggcg                                               >  1:3971783/1‑91 (MQ=255)

                                         tttAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTg                                               <  1:4125582/93‑1 (MQ=255)

                                           tAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGc                                             >  2:3242267/1‑93 (MQ=255)

                                              aTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGcc                                          <  1:3074850/93‑1 (MQ=255)

                                               ttCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCa                                         >  2:4963557/1‑93 (MQ=255)

                                                tCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCAt                                        >  1:2502441/1‑93 (MQ=255)

                                                  cAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTa                                      >  1:1779331/1‑93 (MQ=255)

                                                  cAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTa                                      >  2:2656409/1‑93 (MQ=255)

                                                   agagACTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTAc                                     >  2:4666960/1‑93 (MQ=255)

                                                      gaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGa                                  <  2:1894861/93‑1 (MQ=255)

                                                      gaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGa                                  >  2:4216800/1‑93 (MQ=255)

                                                        cTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGAct                                >  2:3163967/1‑92 (MQ=255)

                                                        cTGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACa                                <  1:330020/93‑1 (MQ=255)

                                                         tGTCGGTTGAAATA‑TAACTAACCCCCTAACGGGGGCATTTCCTCCGATAAATCCAGAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAtt                              <  2:2840293/93‑1 (MQ=255)

                                                         tGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAt                               >  2:351905/1‑93 (MQ=255)

                                                         tGTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAt                               >  1:4682490/1‑93 (MQ=255)

                                                          gTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAtt                              <  2:4918717/93‑1 (MQ=255)

                                                          gTCCGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACAtt                              >  1:938313/1‑93 (MQ=255)

                                                            ccGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTa                            <  1:4553226/93‑1 (MQ=255)

                                                             cGTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTAc                           <  2:303143/93‑1 (MQ=255)

                                                              gTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACg                          <  2:1502581/93‑1 (MQ=255)

                                                              gTTGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACg                          >  2:1169750/1‑93 (MQ=255)

                                                               ttGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGa                         >  2:889837/1‑93 (MQ=255)

                                                               ttGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGa                         <  1:6207843/93‑1 (MQ=255)

                                                               ttGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGa                         >  2:3578610/1‑93 (MQ=255)

                                                                tGAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGAt                        >  1:4535978/1‑93 (MQ=255)

                                                                 gAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATa                       <  2:4648384/93‑1 (MQ=255)

                                                                 gAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATa                       <  1:3550681/93‑1 (MQ=255)

                                                                 gAAATATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATa                       >  2:3217814/1‑93 (MQ=255)

                                                                  aaaTATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATAc                      >  1:5556301/1‑93 (MQ=255)

                                                                   aaTATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATAcc                     <  1:992639/93‑1 (MQ=255)

                                                                   aaTATTAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATAcc                     <  2:764703/93‑1 (MQ=255)

                                                                       ttACCTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTa                 <  1:1738811/93‑1 (MQ=255)

                                                                       ttAACTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTa                 >  2:2065291/1‑93 (MQ=255)

                                                                           cTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaa             >  2:1827470/1‑93 (MQ=255)

                                                                           cTATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaa             >  1:130030/1‑93 (MQ=255)

                                                                            tATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaaa            <  1:5903750/93‑1 (MQ=255)

                                                                            tATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaaa            <  2:5628300/93‑1 (MQ=255)

                                                                            tATCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaaa            >  2:550141/1‑93 (MQ=255)

                                                                             aTCCCCTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCaaaa           >  1:5184038/1‑93 (MQ=255)

                                                                                cccTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTa        >  1:6154521/1‑93 (MQ=255)

                                                                                 ccTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTAc       <  1:5050761/93‑1 (MQ=255)

                                                                                 ccTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTAc       <  2:212301/93‑1 (MQ=255)

                                                                                  cTTACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTAcc      <  1:5222250/93‑1 (MQ=255)

                                                                                   ttACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTAcca     >  2:1642458/1‑92 (MQ=255)

                                                                                   ttACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCg     <  2:1386035/93‑1 (MQ=255)

                                                                                    tACCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGa    <  2:5703255/93‑1 (MQ=255)

                                                                                     tccGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGAc   <  2:2631377/92‑1 (MQ=255)

                                                                                     aCCGGGGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCGCCATTACTGACATTTACGATACCGCTACCAAAATTACCGAc   <  1:5431537/93‑1 (MQ=255)

ATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACA  >  NC_000913/1594430‑1594608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`