Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,666,834 C→T P261P (CCG→CCA)  ynfL ← putative transcriptional regulator LYSR‑type

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,666,8340CT99.5% 760.8 / NA 203P261P (CCG→CCA) ynfLputative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (99/103);  minor base A (1/0);  total (100/103)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

ATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATCGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGA  >  NC_000913/1666744‑1666922
                                                                                          |                                                                                        
aTGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTgg                                                                                        <  2:5362375/93‑1 (MQ=255)
 tGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGc                                                                                       <  1:1190599/93‑1 (MQ=255)
 tGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGc                                                                                       >  1:6288087/1‑93 (MQ=255)
 tGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGc                                                                                       >  2:2238692/1‑93 (MQ=255)
 tGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGc                                                                                       >  2:2533426/1‑93 (MQ=255)
  gAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCa                                                                                      >  2:3023477/1‑93 (MQ=255)
  gAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCa                                                                                      <  2:205237/93‑1 (MQ=255)
   aaTACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCAc                                                                                     >  2:3116286/1‑93 (MQ=255)
     tACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCAccc                                                                                   >  2:3877113/1‑93 (MQ=255)
     tACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCAccc                                                                                   <  2:1470850/93‑1 (MQ=255)
       cGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAg                                                                                 <  1:1326678/93‑1 (MQ=255)
       cGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAg                                                                                 <  1:4537480/93‑1 (MQ=255)
         aaaGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAgcg                                                                               >  2:825799/1‑93 (MQ=255)
          aaGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAgcgc                                                                              <  1:457810/93‑1 (MQ=255)
          aaGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAgcgc                                                                              >  2:1207401/1‑93 (MQ=255)
          aaGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAgcgc                                                                              >  2:2553871/1‑93 (MQ=255)
            gTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCAt                                                                            <  1:4632949/93‑1 (MQ=255)
            gTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCAt                                                                            <  1:2208914/93‑1 (MQ=255)
             ttACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCAtt                                                                           <  1:5666579/93‑1 (MQ=255)
               aCGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTc                                                                         >  2:1750723/1‑93 (MQ=255)
               aCGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTc                                                                         <  1:1599849/93‑1 (MQ=255)
                  cgcAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGtt                                                                      >  2:2118469/1‑93 (MQ=255)
                  cgcAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGtt                                                                      <  1:1691091/93‑1 (MQ=255)
                   gcAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTg                                                                     >  2:3442759/1‑93 (MQ=255)
                    cAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGttca                                                                    <  1:6089715/93‑3 (MQ=255)
                    cAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGa                                                                    <  1:5732203/93‑1 (MQ=255)
                     aGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAg                                                                   >  1:4680047/1‑93 (MQ=255)
                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  >  1:1143306/1‑93 (MQ=255)
                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  <  2:4214287/93‑1 (MQ=255)
                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  >  2:2124846/1‑93 (MQ=255)
                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  >  2:2657526/1‑93 (MQ=255)
                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  >  2:2363886/1‑93 (MQ=255)
                      gCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGc                                                                  <  1:3859310/93‑1 (MQ=255)
                        cGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTg                                                                <  2:8136/93‑1 (MQ=255)
                        cGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTg                                                                >  1:5307186/1‑93 (MQ=255)
                        cGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTg                                                                >  2:1518316/1‑93 (MQ=255)
                          gACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGaa                                                              <  2:6259889/93‑1 (MQ=255)
                           aCTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAac                                                             >  1:1950142/1‑93 (MQ=255)
                            cTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAaca                                                            <  1:4086590/93‑1 (MQ=255)
                            cTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATAGGCACCCAGCGCATTTCGTTGAGCTGAaca                                                            >  2:5445783/1‑93 (MQ=255)
                                gTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGtt                                                        <  1:5129770/93‑1 (MQ=255)
                                 ttCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGttt                                                       >  2:138192/1‑93 (MQ=255)
                                  tCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTa                                                      <  1:3964081/93‑1 (MQ=255)
                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     >  2:3298619/1‑93 (MQ=255)
                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     >  2:3486892/1‑93 (MQ=255)
                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     >  2:2725751/1‑93 (MQ=255)
                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     <  1:3100620/93‑1 (MQ=255)
                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     <  2:3930974/93‑1 (MQ=255)
                                   cATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaa                                                     <  1:2567955/93‑1 (MQ=255)
                                    atgatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaaa                                                    <  1:3262919/93‑1 (MQ=255)
                                    atgatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTaaa                                                    >  1:90682/1‑93 (MQ=255)
                                     tgatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAAt                                                   <  1:1767219/93‑1 (MQ=255)
                                      gatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATg                                                  >  1:498869/1‑93 (MQ=255)
                                      gatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATg                                                  <  2:1557322/93‑1 (MQ=255)
                                      gatgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATg                                                  <  1:1614257/93‑1 (MQ=255)
                                      gatgTTTCGGCCAGAACAACCACATTTCAGAAACCGCATCCTCTTCAGCAACTGGCACCCAGCGCATTTCGTTGAGCTTAGCACGTTAAAATg                                                  >  2:3670485/1‑93 (MQ=255)
                                       atgTTTCGGCCAGACCAACCACATTTCAGAAGCCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGa                                                 <  2:1857713/93‑1 (MQ=255)
                                       atgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGa                                                 >  1:5403300/1‑93 (MQ=255)
                                        tgTTTCGGCCGGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGAc                                                <  2:4826971/93‑1 (MQ=255)
                                        tgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGAc                                                <  2:3394717/93‑1 (MQ=255)
                                        tgTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGAc                                                <  2:3510715/93‑1 (MQ=255)
                                         gTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACg                                               <  1:4709483/93‑1 (MQ=255)
                                          tttCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGc                                              <  2:5706545/93‑1 (MQ=255)
                                             cGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAgg                                           >  2:5953047/1‑93 (MQ=255)
                                                ccAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCaa                                        >  2:4673083/1‑93 (MQ=255)
                                                 cAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCaaa                                       >  1:426536/1‑93 (MQ=255)
                                                 cAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCaaa                                       <  1:2321350/93‑1 (MQ=255)
                                                  aGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCaaaa                                      >  1:2949783/1‑93 (MQ=255)
                                                  aGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCaaaa                                      >  1:638809/1‑93 (MQ=255)
                                                     ccacccacATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTg                                   <  1:2795366/93‑1 (MQ=255)
                                                      caaccaCATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGa                                  >  2:3222332/1‑93 (MQ=255)
                                                       caccaCATTTCAGAAACCGCATCCTCCTCAACAATTGGCCCCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGaa                                 <  1:2645679/92‑1 (MQ=255)
                                                        accaCATTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGaaa                                >  2:6017242/1‑93 (MQ=255)
                                                           acaTTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAacac                             >  2:2030296/1‑93 (MQ=255)
                                                            caTTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACAcc                            >  1:3346623/1‑93 (MQ=255)
                                                             aTTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACAccc                           <  1:3170893/93‑1 (MQ=255)
                                                             aTTTCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACAccc                           <  1:2995174/93‑1 (MQ=255)
                                                              tttCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCa                          <  1:5344882/93‑1 (MQ=255)
                                                              tttCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCa                          >  2:1968946/1‑93 (MQ=255)
                                                              tttCAGAAACCGCAGCCTTTTGAGCAATTGGCACCCAGGGCATTTCGTTGAGCCGAAAGCGTTTAAATGACGCAGGCAAAATTGAACCACCCa                          <  2:1280745/93‑1 (MQ=255)
                                                                tCAGAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGa                        <  1:401816/93‑1 (MQ=255)
                                                                   gAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACcg                     >  1:107248/1‑93 (MQ=255)
                                                                   gAAACCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACcg                     >  1:1552669/1‑93 (MQ=255)
                                                                    aaaCCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACcgg                    >  2:1647595/1‑93 (MQ=255)
                                                                     aaCCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACcggc                   <  2:5801428/93‑1 (MQ=255)
                                                                      aCCGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACcggcg                  >  2:1771525/1‑93 (MQ=255)
                                                                        cGCATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGa                >  1:2280923/1‑93 (MQ=255)
                                                                          cATCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGaaa              <  2:3746155/93‑1 (MQ=255)
                                                                           aTCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAAc             <  2:1031259/93‑1 (MQ=255)
                                                                            tCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAAcc            >  2:1109224/1‑93 (MQ=255)
                                                                            tCCTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAAca            >  1:3637769/1‑92 (MQ=255)
                                                                             ccTCTTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCa           >  2:5970610/1‑93 (MQ=255)
                                                                              ctctTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAg          >  2:1803180/1‑93 (MQ=255)
                                                                               tctTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGa         >  1:1392650/1‑93 (MQ=255)
                                                                               tctTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGa         <  1:2124425/93‑1 (MQ=255)
                                                                                ctTCAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGAc        <  2:3195510/93‑1 (MQ=255)
                                                                                   cAGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCga     >  2:6283951/1‑93 (MQ=255)
                                                                                    aGCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCgat    <  2:5116898/93‑1 (MQ=255)
                                                                                    aGCAATTGGCACCCAACGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCgat    >  2:2847516/1‑93 (MQ=255)
                                                                                     gCAATTGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCgatg   >  2:5505772/1‑93 (MQ=255)
                                                                                          |                                                                                        
ATGAATACGAAAGTTACGCGCAGCCGGACTTTGTTCATGATGTTTCGGCCAGACCAACCACATTTCAGAAACCGCATCCTCTTCAGCAATCGGCACCCAGCGCATTTCGTTGAGCTGAACACGTTTAAATGACGCAGGCAAAATTGAAACACCCAGACCGGCGGAAACCAGACCGATGA  >  NC_000913/1666744‑1666922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑