BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,684,672	A→G	intergenic (‑60/+83)	fumC ← / ← fumA	fumarase C/fumarase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,684,672	0	A	G	99.5%	629.9 / NA	193	intergenic (‑60/+83)	fumC/fumA	fumarase C/fumarase A
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (100/92); minor base C (1/0); total (101/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

TCGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTC  >  NC_000913/1684582‑1684761

  gAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTc                                                                                       >  2:5422060/1‑93 (MQ=255)

  gAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTc                                                                                       <  1:5514643/93‑1 (MQ=255)

    aTCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCAc                                                                                     >  1:3517800/1‑93 (MQ=255)

    aTCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCAc                                                                                     <  2:2717033/93‑1 (MQ=255)

    aTCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCAc                                                                                     <  2:3543699/93‑1 (MQ=255)

      cTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACtt                                                                                   <  2:6171165/93‑1 (MQ=255)

        ttttCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACttat                                                                                 <  1:4736540/93‑1 (MQ=255)

        ttttCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACttat                                                                                 <  2:4228840/93‑1 (MQ=255)

         tttCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACttatt                                                                                <  1:2667899/93‑1 (MQ=255)

             gCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTAttt                                                                            <  1:1099872/93‑1 (MQ=255)

              cTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTa                                                                           >  2:1483373/1‑93 (MQ=255)

              cTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTa                                                                           <  1:2708519/93‑1 (MQ=255)

               tGCGTACTGTATTCATGACCTGCTCCTCACCTGATTATTTTTTTC‑TTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTAcc                                                                         <  1:4348950/93‑1 (MQ=255)

               tGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTAc                                                                          >  1:2288723/1‑93 (MQ=255)

                  gTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCAt                                                                       >  2:5692879/1‑93 (MQ=255)

                  gTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCAt                                                                       <  2:687641/93‑1 (MQ=255)

                   tACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCAtt                                                                      <  2:4410411/93‑1 (MQ=255)

                   tACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCAtt                                                                      >  1:5252313/1‑93 (MQ=255)

                   tACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCAtt                                                                      >  2:2823009/1‑93 (MQ=255)

                    aCTGTTTTCATGCCCTGCTCTTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTA‑TTACCATTTg                                                                    <  2:5001018/93‑1 (MQ=255)

                    aCTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCAttt                                                                     <  1:2170550/93‑1 (MQ=255)

                      tGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGa                                                                   >  2:303921/1‑93 (MQ=255)

                       gTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCGACTTTTGGCTCACTTATTATTTACCATTTGAt                                                                  >  2:3076389/1‑93 (MQ=255)

                       gTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGAt                                                                  <  1:2866668/93‑1 (MQ=255)

                       gTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGAt                                                                  <  1:2653014/93‑1 (MQ=255)

                        tATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATa                                                                 >  1:6120151/1‑93 (MQ=255)

                        tATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATa                                                                 <  2:5776639/93‑1 (MQ=255)

                        tATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATa                                                                 >  1:5588364/1‑93 (MQ=255)

                        tATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATa                                                                 >  1:5467545/1‑93 (MQ=255)

                         aTTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATaa                                                                <  2:3832357/93‑1 (MQ=255)

                          ttCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATagc                                                               <  2:3293749/93‑3 (MQ=255)

                          ttCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAAc                                                               >  1:646130/1‑93 (MQ=255)

                          ttCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAAc                                                               <  2:207179/93‑1 (MQ=255)

                           tCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACa                                                              <  2:4874382/93‑1 (MQ=255)

                           tCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACa                                                              <  1:3664985/93‑1 (MQ=255)

                              tGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAAt                                                           >  1:5641151/1‑93 (MQ=255)

                               gACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATg                                                          >  1:5729661/1‑93 (MQ=255)

                               gACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATg                                                          >  2:2934795/1‑93 (MQ=255)

                               gACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATg                                                          >  1:1882539/1‑93 (MQ=255)

                                 ccTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGtt                                                        <  1:5155942/93‑1 (MQ=255)

                                 ccTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGtt                                                        <  2:2507746/93‑1 (MQ=255)

                                  cTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGttt                                                       <  2:7903/93‑1 (MQ=255)

                                   tGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTg                                                      <  2:3616589/93‑1 (MQ=255)

                                   tGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTg                                                      <  2:1705920/93‑1 (MQ=255)

                                   tGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTg                                                      <  2:2872841/93‑1 (MQ=255)

                                      tcctcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTc                                                   <  1:5313764/93‑1 (MQ=255)

                                      tcctcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTc                                                   <  2:5430072/93‑1 (MQ=255)

                                         tcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCttt                                                <  1:5934330/93‑1 (MQ=255)

                                         tcACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCttt                                                >  2:5110379/1‑93 (MQ=255)

                                         tcACCTGATTAAGTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCttt                                                >  1:3406005/1‑93 (MQ=255)

                                          cACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTc                                               <  1:3235481/93‑1 (MQ=255)

                                          cACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAAAGTTTGGTCTTTc                                               <  1:3404053/93‑1 (MQ=255)

                                            ccTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGt                                             >  1:4329343/1‑93 (MQ=255)

                                            ccTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGt                                             >  2:3232054/1‑93 (MQ=255)

                                             cTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTg                                            >  2:4037052/1‑93 (MQ=255)

                                             cTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTg                                            >  2:4846376/1‑93 (MQ=255)

                                              tGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGc                                           >  2:3996006/1‑93 (MQ=255)

                                               gATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGcc                                          >  1:2960483/1‑93 (MQ=255)

                                                aTTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCa                                         <  2:1904579/93‑1 (MQ=255)

                                                aTTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTCGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCa                                         >  1:4437679/1‑93 (MQ=255)

                                                  tAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATg                                       >  1:628205/1‑93 (MQ=255)

                                                   aaTTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGt                                      >  2:3875240/1‑93 (MQ=255)

                                                      tttttCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTaaa                                   >  2:2378621/1‑93 (MQ=255)

                                                         ttctttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTaaaaaa                                >  2:2019503/1‑93 (MQ=255)

                                                          tctttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTaaaaaaa                               >  2:3428574/1‑93 (MQ=255)

                                                           ctttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAAc                              <  1:2135792/93‑1 (MQ=255)

                                                           ctttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAAc                              <  1:6193418/93‑1 (MQ=255)

                                                            tttctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAAcc                             <  1:4987454/93‑1 (MQ=255)

                                                              tctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGc                           <  2:2627047/93‑1 (MQ=255)

                                                              tctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGc                           >  2:5207266/1‑93 (MQ=255)

                                                              tctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGc                           <  2:446698/93‑1 (MQ=255)

                                                               ctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGcc                          <  2:3276928/93‑1 (MQ=255)

                                                               ctGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGcc                          >  2:5995595/1‑93 (MQ=255)

                                                                tGTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGccc                         <  2:6074372/93‑1 (MQ=255)

                                                                 gTTTTGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGcccc                        <  1:507761/93‑1 (MQ=255)

                                                                    ttGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGaa                     >  2:127375/1‑93 (MQ=255)

                                                                    ttGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGaa                     <  2:1414580/93‑1 (MQ=255)

                                                                    ttGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGaa                     >  1:4759100/1‑93 (MQ=255)

                                                                    ttGCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGaa                     >  2:5273100/1‑93 (MQ=255)

                                                                      gCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAgg                   >  1:1652571/1‑93 (MQ=255)

                                                                      gCTTTCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAgg                   >  2:2130797/1‑93 (MQ=255)

                                                                        tttCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGggc                 >  2:5629035/1‑93 (MQ=255)

                                                                         ttCGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGggcg                >  2:5392600/1‑93 (MQ=255)

                                                                         ttCGTAAAGCATCTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGggcg                <  2:6111487/93‑1 (MQ=255)

                                                                           cGTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGggcggc              <  2:2424231/93‑1 (MQ=255)

                                                                            gTTAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGct             <  1:1435551/93‑1 (MQ=255)

                                                                             ttAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGAGCGGctc            >  1:6044140/1‑93 (MQ=255)

                                                                              tAAGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGctct           <  2:459184/93‑1 (MQ=255)

                                                                               aaGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTg          >  1:1191977/1‑93 (MQ=255)

                                                                               aaGCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTg          <  2:3310199/93‑1 (MQ=255)

                                                                                 gCAACTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGtt        >  1:4296426/1‑93 (MQ=255)

                                                                                   aaCTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTa      >  2:5529197/1‑93 (MQ=255)

                                                                                   aaCTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTa      >  1:5350125/1‑93 (MQ=255)

                                                                                    aCTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTAt     >  1:3978825/1‑93 (MQ=255)

                                                                                    aCTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTAt     <  1:1742913/93‑1 (MQ=255)

                                                                                    aCTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTAt     >  2:5148492/1‑93 (MQ=255)

                                                                                    aCTTTTGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTAt     >  2:2525803/1‑93 (MQ=255)

                                                                                      ttttGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTAttt   >  1:2077460/1‑93 (MQ=255)

                                                                                       tttGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTc  <  2:5158812/93‑1 (MQ=255)

                                                                                       tttGGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTc  >  1:5808912/1‑93 (MQ=255)

TCGAATCTTTTTCGCTGCGTACTGTATTCATGACCTGCTCCTCACCTGATTAATTTTTTCTTTCTGTTTTGCTTTCGTTAAGCAACTTTTAGCTCACTTATTATTTACCATTTGATAACAAATGTTTGGTCTTTCGTGCCATGTAAAAAAACCGCCCCGAAGGGCGGCTCTGTTTATTTC  >  NC_000913/1684582‑1684761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`