BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,695,310	T→C	Q252R (CAG→CGG)	hdhA ←	NAD‑dependent 7alpha‑hydroxysteroid dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,695,310	0	T	C	100.0%	646.6 / NA	201	Q252R (CAG→CGG)	hdhA	NAD‑dependent 7alpha‑hydroxysteroid dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (91/110); total (91/110)

Only 100 of 204 total aligned reads displayed.

CCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCT  >  NC_000913/1695220‑1695399

 cAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGGCCGTTAGTGTATTAATTGAGCTCCCGTa                                                                                        >  1:4781128/1‑93 (MQ=255)

  aGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAc                                                                                       >  1:4203861/1‑93 (MQ=255)

  aGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAc                                                                                       <  2:3619878/93‑1 (MQ=255)

  aGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAc                                                                                       <  2:5025848/93‑1 (MQ=255)

     tATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTAcccc                                                                                    >  1:5008945/1‑93 (MQ=255)

       tttAGCAGAGATTTTGATGAGTTGATCCAGGTAAACAACACGCACGGTTGTTTACCGGTCCGTGAGTGTATTAATTGAGCTCCCGTACCccac                                                                                  <  2:2011010/93‑1 (MQ=255)

       tttAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCccac                                                                                  <  2:3218034/93‑1 (MQ=255)

         tAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCccacca                                                                                <  1:1244244/93‑1 (MQ=255)

         tAGAAGAGACGTAGATGAGTTTATCCCGGTAAACAACACGCACGATTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTTCCccacca                                                                                <  2:4940101/93‑1 (MQ=255)

          aGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCcaccac                                                                               <  1:178791/93‑1 (MQ=255)

          aGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCcaccac                                                                               <  1:3043317/93‑1 (MQ=255)

             agagACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCgg                                                                            >  2:1949424/1‑93 (MQ=255)

             agagACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCgg                                                                            <  1:3377701/93‑1 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           >  2:3711063/1‑93 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           <  2:5837772/93‑1 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           >  2:1633113/1‑93 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           >  2:4217876/1‑93 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGga                                                                           <  2:5895307/93‑1 (MQ=255)

              gagaCGTTGATGAGTTTATCCCGGCAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGGATTAATTGAGCTCCCGTACCCCACCAACGga                                                                           >  2:3810496/1‑93 (MQ=255)

               agaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGgag                                                                          <  1:4950237/93‑1 (MQ=255)

                gaCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGgaga                                                                         <  1:5261101/93‑1 (MQ=255)

                 aCGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGAc                                                                        <  2:4236591/93‑1 (MQ=255)

                   gTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACgg                                                                      <  1:9618/93‑1 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACggg                                                                     >  1:5658850/1‑92 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGt                                                                     <  1:1381671/93‑1 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGt                                                                     >  1:6181606/1‑93 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGt                                                                     <  1:4342940/93‑1 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGt                                                                     >  2:5802237/1‑93 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGt                                                                     <  2:5180719/93‑1 (MQ=255)

                    ttGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCACACCACCGGAGACggg                                                                     >  1:5133220/1‑92 (MQ=255)

                     tgatgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTg                                                                    >  1:5789174/1‑93 (MQ=255)

                      gatgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTga                                                                   >  1:2940180/1‑93 (MQ=255)

                      gatgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTga                                                                   >  1:501455/1‑93 (MQ=255)

                      gatgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTga                                                                   >  1:47264/1‑93 (MQ=255)

                       atgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTgag                                                                  <  1:3514079/93‑1 (MQ=255)

                        tgaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTgaga                                                                 <  2:783503/93‑1 (MQ=255)

                         gaGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGaa                                                                <  2:170462/93‑1 (MQ=255)

                          aGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAAt                                                               <  2:4681944/93‑1 (MQ=255)

                            tttATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAAttt                                                             <  1:1956730/93‑1 (MQ=255)

                                tCCCGGTAAACACCACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTcc                                                         <  2:3569094/93‑1 (MQ=255)

                                tCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTcc                                                         >  2:604997/1‑93 (MQ=255)

                                tCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTcc                                                         <  2:458822/93‑1 (MQ=255)

                                   cGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCt                                                      >  1:4556853/1‑93 (MQ=255)

                                    ggTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGCGACGGTGAGAATTTGTCCGCtt                                                     >  2:1148201/1‑93 (MQ=255)

                                     gTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTa                                                    <  1:5300301/93‑1 (MQ=255)

                                     gTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTa                                                    >  1:3232266/1‑93 (MQ=255)

                                      tAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAc                                                   <  2:4773131/93‑1 (MQ=255)

                                      aaaaCAACACGCACGGTGGTTAGCCGGTCCGTTAGTGTATTAATTGAGCTCCCGTGCCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAc                                                   <  1:3930713/92‑1 (MQ=255)

                                       aaaCAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAcc                                                  >  2:6244168/1‑93 (MQ=255)

                                        aacaacACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTAccc                                                 <  1:2675517/93‑1 (MQ=255)

                                         acaacaCGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCa                                                >  2:3964748/1‑93 (MQ=255)

                                            acacGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCt                                             >  1:581593/1‑93 (MQ=255)

                                            acacGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCt                                             <  1:6034209/93‑1 (MQ=255)

                                                gcacgGTTGTTAGCAGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCgca                                         <  2:2694390/93‑1 (MQ=255)

                                                    ggTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGTCGGTGAGACTTTGTCCGCTTAGCCAGCTCGccgcag                                     >  2:2312791/1‑93 (MQ=255)

                                                    ggTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGcagcag                                     <  2:3097182/93‑1 (MQ=255)

                                                    ggTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGcagcag                                     <  2:3889893/93‑1 (MQ=255)

                                                    ggTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGcagcag                                     <  1:3149650/93‑1 (MQ=255)

                                                     gttgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAgg                                    <  1:1482315/93‑1 (MQ=255)

                                                      ttgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGc                                   <  2:4452270/93‑1 (MQ=255)

                                                      ttgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGc                                   <  1:5972053/93‑1 (MQ=255)

                                                        gttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGa                                 >  2:5536953/1‑93 (MQ=255)

                                                        gttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGa                                 >  1:851013/1‑93 (MQ=255)

                                                        gttTACCGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGa                                 >  1:2332906/1‑93 (MQ=255)

                                                             ccGGTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCaaa                            <  1:2438806/93‑1 (MQ=255)

                                                               ggTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGCGCACAgg                          >  1:4820006/1‑93 (MQ=255)

                                                               ggTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg                          >  2:2972282/1‑93 (MQ=255)

                                                                gTCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGa                         >  1:3296312/1‑93 (MQ=255)

                                                                 tCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGaa                        >  2:2209200/1‑93 (MQ=255)

                                                                 tCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGaa                        <  1:1968278/93‑1 (MQ=255)

                                                                 tCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGaa                        <  2:3318159/93‑1 (MQ=255)

                                                                 tCCGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGaa                        <  1:3683985/93‑1 (MQ=255)

                                                                  ccGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAAc                       >  1:1050116/1‑93 (MQ=255)

                                                                  ccGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAACTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAAc                       <  2:5135054/93‑1 (MQ=255)

                                                                   cGTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACa                      <  1:1562079/93‑1 (MQ=255)

                                                                    gTTAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAg                     >  1:4597774/1‑93 (MQ=255)

                                                                     ttAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgc                    <  2:3114575/93‑1 (MQ=255)

                                                                      tAGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcg                   >  1:648174/1‑93 (MQ=255)

                                                                       aGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCTCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcgc                  <  2:663415/93‑1 (MQ=255)

                                                                       aGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcgc                  <  1:4247851/93‑1 (MQ=255)

                                                                       aGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcgc                  >  2:2142503/1‑93 (MQ=255)

                                                                       aGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcgc                  <  1:2864612/93‑1 (MQ=255)

                                                                       aGTGTATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAgcgc                  <  2:565188/93‑1 (MQ=255)

                                                                        gtgtATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCt                 <  1:2221373/93‑1 (MQ=255)

                                                                         tgtATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGATTTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTg                <  1:2044513/93‑1 (MQ=255)

                                                                          gtATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGc               <  1:5594050/93‑1 (MQ=255)

                                                                          gtATTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGc               >  1:3087388/1‑93 (MQ=255)

                                                                            aTTAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGt             <  2:4099335/93‑1 (MQ=255)

                                                                             ttAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGtt            >  1:1915787/1‑93 (MQ=255)

                                                                             ttAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGtt            <  2:1909318/93‑1 (MQ=255)

                                                                              tAATTGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTa           <  1:1633113/93‑1 (MQ=255)

                                                                                 ttGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCa        <  2:1483719/93‑1 (MQ=255)

                                                                                  tGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCaa       <  2:2611780/93‑1 (MQ=255)

                                                                                  tGAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCaa       >  2:6146802/1‑93 (MQ=255)

                                                                                   gAGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAat      <  1:2558072/93‑1 (MQ=255)

                                                                                    aGCTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAata     <  2:5924762/93‑1 (MQ=255)

                                                                                      cTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATc   <  2:1663514/93‑1 (MQ=255)

                                                                                      cTCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATc   <  2:4458802/93‑1 (MQ=255)

                                                                                       tCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCt  <  1:4480070/93‑1 (MQ=255)

                                                                                       tCCCGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCt  >  1:168102/1‑93 (MQ=255)

CCAGTTATTTAGCAGAGACGTTGATGAGTTTATCCCGGTAAACAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCT  >  NC_000913/1695220‑1695399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`