BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,758,261	T→C	E96G (GAA→GGA)	b1680 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,758,261	0	T	C	99.4%	550.8 / NA	173	E96G (GAA→GGA)	b1680	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (91/81); minor base G (1/0); total (92/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

TTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCG  >  NC_000913/1758171‑1758350

ttAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGAtccctccc                                                                                         <  2:3895784/93‑1 (MQ=255)

 tAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCcg                                                                                        >  2:2154049/1‑93 (MQ=255)

  aGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCcgt                                                                                       <  1:3112203/93‑1 (MQ=255)

    cGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCcgtcg                                                                                     >  1:4246852/1‑93 (MQ=255)

     gtggtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCcgtcgt                                                                                    >  1:4015440/1‑93 (MQ=255)

        gtgCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGcc                                                                                 <  1:4565181/93‑1 (MQ=255)

          gCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCcgc                                                                               >  1:3404331/1‑93 (MQ=255)

           cTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCcgcg                                                                              >  2:5791593/1‑93 (MQ=255)

            tCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGTCCAGATTGATCCCTCCCGTCGTGCCGCgg                                                                             <  2:4004914/93‑1 (MQ=255)

            tCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCgg                                                                             >  2:1034716/1‑93 (MQ=255)

            tCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCgg                                                                             >  1:4982776/1‑93 (MQ=255)

             ccATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGa                                                                            <  1:2801344/93‑1 (MQ=255)

             ccATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGa                                                                            >  2:5002652/1‑93 (MQ=255)

             ccATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCGGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGa                                                                            >  1:3378882/1‑93 (MQ=255)

                tCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGa                                                                         >  2:1610265/1‑93 (MQ=255)

                  tgactgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCTCGGACGAac                                                                       >  2:2862550/1‑93 (MQ=255)

                   gactgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAaca                                                                      <  2:187873/93‑1 (MQ=255)

                     ctgaTGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACAcc                                                                    <  2:969170/93‑1 (MQ=255)

                      tgatgatgaTGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCa                                                                   <  1:4229391/93‑1 (MQ=255)

                       gatgatgatGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAg                                                                  <  1:5472060/93‑1 (MQ=255)

                       gatgatgatGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAg                                                                  >  1:476406/1‑93 (MQ=255)

                       gatgatgatGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAg                                                                  >  1:4284824/1‑93 (MQ=255)

                        atgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTGCCGTCGTGCCGCGGACGAACACCAGc                                                                 >  1:1046278/1‑93 (MQ=255)

                        atgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGc                                                                 >  2:809859/1‑93 (MQ=255)

                         tgatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGct                                                                >  2:2666271/1‑93 (MQ=255)

                          gatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGctc                                                               <  1:5638488/93‑1 (MQ=255)

                          gatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGctc                                                               <  1:3256614/93‑1 (MQ=255)

                          gatgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGctc                                                               >  2:4352080/1‑93 (MQ=255)

                           atgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGctct                                                              >  1:864855/1‑93 (MQ=255)

                            tgatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAGCACCCGCTCtt                                                             >  2:3306498/1‑93 (MQ=255)

                             gatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTc                                                            <  1:2589513/93‑1 (MQ=255)

                             gatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTc                                                            <  2:4047648/93‑1 (MQ=255)

                             gatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTc                                                            >  1:5392388/1‑93 (MQ=255)

                             gatgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTc                                                            <  2:1320285/93‑1 (MQ=255)

                              atgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTcc                                                           >  1:3911368/1‑93 (MQ=255)

                              atgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTcc                                                           <  2:288460/93‑1 (MQ=255)

                               tgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTccg                                                          <  1:3884678/93‑1 (MQ=255)

                               tgTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTccg                                                          >  1:3725788/1‑93 (MQ=255)

                                   aTCTCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACGCCAGCTCTTCCGCCGa                                                      <  2:6282082/93‑1 (MQ=255)

                                    tCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGaa                                                     >  2:494246/1‑93 (MQ=255)

                                    tCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGaa                                                     <  2:1103016/93‑1 (MQ=255)

                                    tCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGaa                                                     >  2:1504282/1‑93 (MQ=255)

                                    tCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGaa                                                     <  1:5917623/93‑1 (MQ=255)

                                     cgcccgccCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAAc                                                    >  2:2657017/1‑93 (MQ=255)

                                      gcccgcccGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACg                                                   <  2:4211388/93‑1 (MQ=255)

                                        ccgcccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACggg                                                 >  2:3154457/1‑93 (MQ=255)

                                        ccgcccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACggg                                                 <  1:1613455/93‑1 (MQ=255)

                                         cgcccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGc                                                <  2:5364497/93‑1 (MQ=255)

                                          gcccgcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCa                                               <  2:3934933/93‑1 (MQ=255)

                                              gcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaa                                           <  2:5221848/93‑1 (MQ=255)

                                              gcACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaa                                           <  2:4717123/93‑1 (MQ=255)

                                               cACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaat                                          >  1:2458828/1‑93 (MQ=255)

                                                aCGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaata                                         <  1:4726443/93‑1 (MQ=255)

                                                aCGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaata                                         >  2:4890156/1‑93 (MQ=255)

                                                aCGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATtaata                                         <  2:1713849/93‑1 (MQ=255)

                                                  gTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATaaa                                       >  1:6235566/1‑93 (MQ=255)

                                                  gTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATaaa                                       >  1:1345753/1‑93 (MQ=255)

                                                  gTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATaaa                                       >  1:5075061/1‑93 (MQ=255)

                                                   ttGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAAc                                      >  2:2623906/1‑93 (MQ=255)

                                                   ttGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAAc                                      <  2:3049851/93‑1 (MQ=255)

                                                   ttGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAAc                                      <  1:3057614/93‑1 (MQ=255)

                                                    tGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACa                                     <  1:1534526/93‑1 (MQ=255)

                                                    tGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACa                                     <  2:2767824/93‑1 (MQ=255)

                                                    tGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACa                                     <  1:3319269/93‑1 (MQ=255)

                                                    tGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACa                                     <  2:3375413/93‑1 (MQ=255)

                                                        gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGATCACCAGCTCTTCCGCCGAACGGGCATTAATAAACAgaga                                 >  2:2313321/1‑93 (MQ=255)

                                                        gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGa                                 >  1:3229878/1‑93 (MQ=255)

                                                        gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGa                                 <  1:3133178/93‑1 (MQ=255)

                                                        gTTGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGa                                 <  2:5687643/93‑1 (MQ=255)

                                                         ttGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGAt                                <  2:2425064/93‑1 (MQ=255)

                                                          tGCCCCAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATg                               >  2:1741239/1‑93 (MQ=255)

                                                             cccAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGccc                            <  1:1712353/93‑1 (MQ=255)

                                                             cacAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGccc                            <  1:5324331/91‑1 (MQ=255)

                                                              ccAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCg                           >  1:2979362/1‑93 (MQ=255)

                                                               cAGCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGc                          <  1:2687231/93‑1 (MQ=255)

                                                                 gCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCtt                        <  2:5825441/93‑1 (MQ=255)

                                                                 gCTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCtt                        >  2:921144/1‑93 (MQ=255)

                                                                  cTATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTg                       >  1:3499510/1‑93 (MQ=255)

                                                                   tATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgc                      <  2:4632752/93‑1 (MQ=255)

                                                                   tATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgc                      >  1:6233338/1‑93 (MQ=255)

                                                                   tATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgc                      >  2:3808034/1‑93 (MQ=255)

                                                                   tATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgc                      >  2:6076007/1‑93 (MQ=255)

                                                                   tATTGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgc                      <  1:3776034/93‑1 (MQ=255)

                                                                     ttGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTgcgc                    <  1:2124314/93‑1 (MQ=255)

                                                                      tGGCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCa                   >  1:250969/1‑93 (MQ=255)

                                                                        gCGACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACg                 <  1:5200499/93‑1 (MQ=255)

                                                                          gACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGtt               <  2:1808917/93‑1 (MQ=255)

                                                                          gACCAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGtt               >  1:2380994/1‑93 (MQ=255)

                                                                           acgaGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTtc              <  1:3239338/90‑1 (MQ=255)

                                                                             cAGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTtctc            <  1:5536162/93‑1 (MQ=255)

                                                                              aGATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTcc           <  2:3674201/93‑1 (MQ=255)

                                                                               gATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCa          <  2:6196535/93‑1 (MQ=255)

                                                                               gATTGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCa          >  2:67335/1‑93 (MQ=255)

                                                                                  tGATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAttt       >  1:1092596/1‑93 (MQ=255)

                                                                                   gATCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAtttt      >  2:425900/1‑93 (MQ=255)

                                                                                    aTCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtc     <  1:1771012/93‑1 (MQ=255)

                                                                                    aTCCCTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGCTCTCCAttttt     >  1:3317419/1‑92 (MQ=255)

                                                                                     tccctcccGTCGTGCCGCGGACGAACACCAGGTCTTACGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCAtttttt    >  2:4475849/1‑91 (MQ=255)

                                                                                     tccctcccGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTtct    >  2:6218210/1‑93 (MQ=255)

                                                                                       ccTCCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCg  <  1:4518496/93‑1 (MQ=255)

TTAGCGTGGTGCTCCATCTGACTGATGATGATGTTATCGCCCGCCCGCACGTTGCTGTTGCCCCAGCTATTGGCGACCAGATTGATCCCTTCCGTCGTGCCGCGGACGAACACCAGCTCTTCCGCCGAACGGGCATTAATAAACAGCGATGCCCGCTTGCGCACGTTCTCCATTTTCTCG  >  NC_000913/1758171‑1758350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`