BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,784,236	C→T	E301K (GAA→AAA)	ppsA ←	phosphoenolpyruvate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,784,236	0	C	T	100.0%	749.1 / NA	200	E301K (GAA→AAA)	ppsA	phosphoenolpyruvate synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (103/97); total (103/97)

Only 100 of 207 total aligned reads displayed.

ACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCG  >  NC_000913/1784146‑1784325

aCAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCt                                                                                         >  2:2607367/1‑93 (MQ=255)

aCAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCt                                                                                         <  2:5724694/93‑1 (MQ=255)

aCAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCt                                                                                         >  1:292155/1‑93 (MQ=255)

aCAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACAGCCTGTTTTGCCAGTTTCt                                                                                         >  2:496300/1‑93 (MQ=255)

 cAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTg                                                                                        <  1:1019198/93‑1 (MQ=255)

 cAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTAAGGCCTGTTTTGCCAGTTTCTg                                                                                        >  2:2710424/1‑93 (MQ=255)

   gTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCa                                                                                      <  2:4343580/93‑1 (MQ=255)

   gTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCa                                                                                      <  1:1709676/93‑1 (MQ=255)

    tttACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCAc                                                                                     >  1:2959870/1‑93 (MQ=255)

    attACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCAc                                                                                     <  1:6222816/92‑1 (MQ=255)

     ttACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCAct                                                                                    <  2:50718/93‑1 (MQ=255)

         cGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCActtctt                                                                                <  2:1729549/93‑1 (MQ=255)

          ggTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACttcttc                                                                               <  1:2293916/93‑1 (MQ=255)

           gtgtGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCg                                                                              >  1:3047304/1‑93 (MQ=255)

            tgtgGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGTCCTGTTTTGCCAGTTTCTGCACTTCTTCGt                                                                             >  1:929955/1‑93 (MQ=255)

             gtgGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGtt                                                                            <  2:3654153/93‑1 (MQ=255)

               ggCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTgg                                                                          >  1:2252239/1‑93 (MQ=255)

                 ccATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTc                                                                        >  1:543139/1‑93 (MQ=255)

                 ccATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTc                                                                        <  1:1825935/93‑1 (MQ=255)

                  cATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCa                                                                       >  1:3347679/1‑93 (MQ=255)

                   aTCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAg                                                                      <  1:4741328/93‑1 (MQ=255)

                    tCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGc                                                                     >  1:1902628/1‑93 (MQ=255)

                    tCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGc                                                                     <  2:459939/93‑1 (MQ=255)

                    tCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGc                                                                     >  2:645908/1‑93 (MQ=255)

                         cGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGagaa                                                                <  2:3547511/93‑1 (MQ=255)

                          gCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGagaag                                                               >  2:3148611/1‑93 (MQ=255)

                           cccACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGagaaga                                                              <  2:2492428/93‑1 (MQ=255)

                            ccACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGAt                                                             >  1:5382703/1‑93 (MQ=255)

                             cACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATg                                                            >  2:3603366/1‑93 (MQ=255)

                             cACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATg                                                            >  2:3772538/1‑93 (MQ=255)

                              aCTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGt                                                           >  1:4305763/1‑93 (MQ=255)

                               cTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGCTGTc                                                          >  1:3854209/1‑93 (MQ=255)

                               cTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTc                                                          >  1:5595795/1‑93 (MQ=255)

                               cTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTc                                                          <  2:5731682/93‑1 (MQ=255)

                               cTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTc                                                          <  1:4961216/93‑1 (MQ=255)

                               cTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTc                                                          >  1:234559/1‑93 (MQ=255)

                                tCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCa                                                         <  1:6120809/93‑1 (MQ=255)

                                tCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCa                                                         <  2:4628132/93‑1 (MQ=255)

                                tCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCa                                                         >  1:273081/1‑93 (MQ=255)

                                 cAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCAc                                                        <  2:2189994/93‑1 (MQ=255)

                                 cAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCAc                                                        <  2:3996387/93‑1 (MQ=255)

                                  aaTATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACg                                                       >  1:4031346/1‑93 (MQ=255)

                                   atatCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGc                                                      <  2:6284685/93‑1 (MQ=255)

                                    tatCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCt                                                     >  1:1486812/1‑93 (MQ=255)

                                     atCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTg                                                    >  2:1360366/1‑93 (MQ=255)

                                     atCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTg                                                    >  1:1180877/1‑93 (MQ=255)

                                       ccATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGtt                                                  >  1:1283792/1‑93 (MQ=255)

                                          tCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCt                                               <  2:4756942/93‑1 (MQ=255)

                                              gCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCgg                                           <  1:5004205/93‑1 (MQ=255)

                                              gCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCgg                                           <  1:3804630/93‑1 (MQ=255)

                                              gCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCgg                                           <  2:1028867/93‑1 (MQ=255)

                                               cGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGt                                          <  1:920946/93‑1 (MQ=255)

                                                 aCCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGCAGATGTCACGCTGTTCCTGCGGTAc                                        >  1:1557300/1‑93 (MQ=255)

                                                 aCCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTAc                                        >  2:4514779/1‑93 (MQ=255)

                                                     tAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCt                                    >  2:3015993/1‑93 (MQ=255)

                                                     tAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCt                                    >  2:1495284/1‑93 (MQ=255)

                                                     tAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCt                                    >  1:2166570/1‑93 (MQ=255)

                                                     tAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCt                                    >  1:4123676/1‑93 (MQ=255)

                                                      aGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCtt                                   <  2:5065780/93‑1 (MQ=255)

                                                      aGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCtt                                   >  2:5056836/1‑93 (MQ=255)

                                                       gtgtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTc                                  <  1:3178676/93‑1 (MQ=255)

                                                       gtgtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTc                                  >  1:3468063/1‑93 (MQ=255)

                                                       gtgtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTc                                  >  2:3863729/1‑93 (MQ=255)

                                                        tgtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCg                                 <  1:4359263/93‑1 (MQ=255)

                                                         gtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGa                                >  2:842894/1‑93 (MQ=255)

                                                         gtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGa                                >  2:551324/1‑93 (MQ=255)

                                                          tttCTCAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGAt                               >  2:3295305/1‑93 (MQ=255)

                                                            tctcAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGAttt                             <  2:4113776/93‑1 (MQ=255)

                                                            tctcAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGAttt                             >  1:469530/1‑93 (MQ=255)

                                                               cAATTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTaa                          >  1:4472775/1‑93 (MQ=255)

                                                                aaTTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAAc                         <  2:3972015/93‑1 (MQ=255)

                                                                 aTTTGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAAcc                        <  1:4631442/93‑1 (MQ=255)

                                                                  tttGTGCGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCt                       <  1:3093777/93‑1 (MQ=255)

                                                                  tttGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCt                       <  2:5343913/93‑1 (MQ=255)

                                                                  tttGTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCt                       >  2:3257409/1‑93 (MQ=255)

                                                                     gTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCt                    <  2:1675868/93‑1 (MQ=255)

                                                                     gTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCt                    >  1:5950850/1‑93 (MQ=255)

                                                                     gTACGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCt                    <  2:2110326/93‑1 (MQ=255)

                                                                        cGGCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGc                 <  1:716321/93‑1 (MQ=255)

                                                                         ggCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGcc                >  2:4220076/1‑93 (MQ=255)

                                                                         ggCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGcc                >  1:37435/1‑93 (MQ=255)

                                                                         ggCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGcc                >  2:231287/1‑93 (MQ=255)

                                                                          gCCTGTTTTGCCAGTTTCTTCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCg               <  2:2572141/93‑1 (MQ=255)

                                                                          gCCTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCg               >  2:5126001/1‑93 (MQ=255)

                                                                           ccTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGt              <  2:3387022/93‑1 (MQ=255)

                                                                           ccTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGt              >  2:5878058/1‑93 (MQ=255)

                                                                            cTGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTg             >  2:2771661/1‑93 (MQ=255)

                                                                             tGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGc            >  2:3050632/1‑93 (MQ=255)

                                                                             tGTTTTGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGc            >  2:1922343/1‑93 (MQ=255)

                                                                               ttttGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTc          >  1:5847969/1‑93 (MQ=255)

                                                                               ttttGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTc          <  1:142898/93‑1 (MQ=255)

                                                                                  tGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTg       <  2:1467907/93‑1 (MQ=255)

                                                                                  tGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTg       <  2:5649607/93‑1 (MQ=255)

                                                                                  tGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTg       <  1:5160575/93‑1 (MQ=255)

                                                                                  tGCCAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTg       <  2:2065049/93‑1 (MQ=255)

                                                                                     cAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGt    <  2:342420/93‑1 (MQ=255)

                                                                                     cAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGt    <  1:4866145/93‑1 (MQ=255)

                                                                                     cAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGt    >  2:3685243/1‑93 (MQ=255)

                                                                                     cAGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGt    <  2:6092326/93‑1 (MQ=255)

                                                                                      aGTTTCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTc   <  1:653718/93‑1 (MQ=255)

ACAGTTTACCGGTGTGGCCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCG  >  NC_000913/1784146‑1784325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`