BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	217,572	T→C	R402G (AGG→GGG)	proS ←	proline tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	217,572	0	T	C	99.6%	749.5 / ‑4.9	238	R402G (AGG→GGG)	proS	proline tRNA synthetase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (115/122); total (115/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.59e-01

Only 100 of 244 total aligned reads displayed.

CACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGC  >  NC_000913/217482‑217661

cACCCTGTACGGAGTCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTAc                                                                                         <  1:3363788/93‑1 (MQ=255)

 aCCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCAcc                                                                                        >  2:2455607/1‑93 (MQ=255)

   ccTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCt                                                                                      <  1:354340/93‑1 (MQ=255)

    cTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTg                                                                                     >  2:2747359/1‑93 (MQ=255)

    cTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTg                                                                                     <  1:2797009/93‑1 (MQ=255)

     tGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGGACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACACTgg                                                                                    >  2:4626371/1‑93 (MQ=255)

       tACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGcc                                                                                  >  2:5686684/1‑93 (MQ=255)

        tcGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCa                                                                                 <  1:1654590/92‑1 (MQ=255)

        aCGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCa                                                                                 >  1:4879210/1‑93 (MQ=255)

         cGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCAt                                                                                >  2:203085/1‑93 (MQ=255)

          ggAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATc                                                                               >  2:3704504/1‑93 (MQ=255)

           gAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATcc                                                                              <  1:667233/93‑1 (MQ=255)

           gAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATcc                                                                              >  2:6197236/1‑93 (MQ=255)

            aGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCg                                                                             <  2:3767142/93‑1 (MQ=255)

             ggCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGCTACCACGTTTGATCAGCAGCCCACCCTCGCCATCCgg                                                                            >  1:6030132/1‑93 (MQ=255)

             ggCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCgg                                                                            >  1:5794321/1‑93 (MQ=255)

             ggCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCgg                                                                            >  1:4823619/1‑93 (MQ=255)

             ggCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCgg                                                                            <  2:1999281/93‑1 (MQ=255)

               cTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGc                                                                          <  2:1481081/93‑1 (MQ=255)

               cTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGc                                                                          >  1:3215267/1‑93 (MQ=255)

                tttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCt                                                                         >  1:4626110/1‑93 (MQ=255)

                      tGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATc                                                                   <  1:5437951/93‑1 (MQ=255)

                      tGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATc                                                                   <  1:428645/93‑1 (MQ=255)

                       gCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCg                                                                  >  2:3141359/1‑93 (MQ=255)

                         ttCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCgcc                                                                <  1:1084533/93‑1 (MQ=255)

                          tCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCgcca                                                               >  1:297057/1‑93 (MQ=255)

                              aGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCgccagcca                                                           <  2:803985/93‑1 (MQ=255)

                              aGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCgccagcca                                                           <  1:1746264/93‑1 (MQ=255)

                               gTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGccac                                                          >  2:312681/1‑93 (MQ=255)

                               gTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGccac                                                          >  2:447217/1‑93 (MQ=255)

                                 aCTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGccacca                                                        <  1:3951323/93‑1 (MQ=255)

                                 aCTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGccacca                                                        <  2:4743368/93‑1 (MQ=255)

                                  cTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCcaccac                                                       >  1:4438506/1‑93 (MQ=255)

                                  cTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCcaccac                                                       >  2:3002326/1‑93 (MQ=255)

                                     ggTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGtt                                                    <  2:1685422/93‑1 (MQ=255)

                                      gTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTa                                                   >  1:3005921/1‑93 (MQ=255)

                                      gTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTa                                                   >  2:6035540/1‑93 (MQ=255)

                                       tACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTAc                                                  <  2:5259803/93‑1 (MQ=255)

                                       tACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTAc                                                  <  1:2165347/93‑1 (MQ=255)

                                        aCCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACg                                                 >  1:5835431/1‑93 (MQ=255)

                                        aCCCAGCTGGAAGAGGTGACCAACTTCGATGCCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACg                                                 <  1:2374401/93‑1 (MQ=255)

                                          ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGa                                               >  2:4124055/1‑93 (MQ=255)

                                          ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGa                                               <  2:4702205/93‑1 (MQ=255)

                                            aGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGata                                             <  2:3028768/93‑1 (MQ=255)

                                             gCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGatat                                            <  1:1860145/93‑1 (MQ=255)

                                               tGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCt                                          >  2:4972330/1‑93 (MQ=255)

                                                ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTg                                         >  2:5717567/1‑93 (MQ=255)

                                                ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTg                                         >  1:274137/1‑93 (MQ=255)

                                                ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTg                                         >  1:5601650/1‑93 (MQ=255)

                                                  aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCa                                       >  2:661152/1‑93 (MQ=255)

                                                   aGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCaa                                      <  1:5233752/93‑1 (MQ=255)

                                                   aGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCaa                                      >  2:4946686/1‑93 (MQ=255)

                                                       gtgACCATCTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTc                                  <  2:5577437/93‑1 (MQ=255)

                                                       gtgACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTc                                  <  2:195807/93‑1 (MQ=255)

                                                       gtgACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTc                                  <  1:6252755/93‑1 (MQ=255)

                                                        tgACCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTcc                                 >  1:4063629/1‑93 (MQ=255)

                                                          aCCAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCgg                               <  2:3276867/93‑1 (MQ=255)

                                                           ccAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCggg                              <  1:1449806/93‑1 (MQ=255)

                                                           ccAACTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCggg                              <  2:1365038/93‑1 (MQ=255)

                                                             aaCTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGt                            >  1:5139957/1‑93 (MQ=255)

                                                             aaCTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGt                            <  2:5285774/93‑1 (MQ=255)

                                                             aaCTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGt                            >  2:5965093/1‑93 (MQ=255)

                                                             aaCTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGt                            <  2:3245593/93‑1 (MQ=255)

                                                             aaCTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGt                            <  2:5199559/93‑1 (MQ=255)

                                                              aCTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTa                           <  2:67863/93‑1 (MQ=255)

                                                              aCTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTACAACTTCCGGGGTa                           >  1:2859105/1‑93 (MQ=255)

                                                               cTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAg                          <  2:6252172/93‑1 (MQ=255)

                                                               cTTCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAg                          <  1:5959456/93‑1 (MQ=255)

                                                                ttCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGc                         <  2:734758/93‑1 (MQ=255)

                                                                 tCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCg                        >  2:5408184/1‑93 (MQ=255)

                                                                 tCGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCg                        <  2:2111244/93‑1 (MQ=255)

                                                                  cGATACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGa                       <  2:1037702/93‑1 (MQ=255)

                                                                    aTACCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACa                     >  2:4269441/1‑93 (MQ=255)

                                                                      aCCACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATc                   >  1:4587055/1‑93 (MQ=255)

                                                                       ccACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcg                  <  2:2244247/93‑1 (MQ=255)

                                                                        cACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgc                 <  1:916589/93‑1 (MQ=255)

                                                                        cACGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgc                 <  2:1162319/93‑1 (MQ=255)

                                                                         aCGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgcg                >  1:2392739/1‑93 (MQ=255)

                                                                         aCGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgcg                >  2:866746/1‑93 (MQ=255)

                                                                         aCGTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgcg                <  1:5251083/93‑1 (MQ=255)

                                                                           gTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGAt              >  1:316058/1‑93 (MQ=255)

                                                                           gTTTGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGAt              <  1:2464434/93‑1 (MQ=255)

                                                                            tttGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATc             >  2:3199766/1‑93 (MQ=255)

                                                                             ttGATCAGCAGTCCACCCTGTCCAGCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATcc            <  2:4192415/93‑1 (MQ=255)

                                                                             ttGATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATcc            <  1:1018298/93‑1 (MQ=255)

                                                                               ggtCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCa          >  2:3973493/3‑93 (MQ=255)

                                                                               gaccagcagCCCCCCCTGGCCATCCGGGCTTGGATCCCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCa          <  2:340458/90‑1 (MQ=255)

                                                                               gATCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCa          <  1:3864251/93‑1 (MQ=255)

                                                                                 tCAGCAGCCCCCCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGt        <  2:2391922/93‑1 (MQ=255)

                                                                                 tCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGt        >  1:2530951/1‑93 (MQ=255)

                                                                                 tCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGt        <  2:3475427/93‑1 (MQ=255)

                                                                                 tCAGCAGCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGt        <  2:2847239/93‑1 (MQ=255)

                                                                                  cagcagCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGtt       <  2:59895/93‑1 (MQ=255)

                                                                                  cagcagCCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGtt       <  2:2613502/93‑1 (MQ=255)

                                                                                   agcagcCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTg      >  2:4570877/1‑93 (MQ=255)

                                                                                    gcagcCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa     <  2:6116089/93‑1 (MQ=255)

                                                                                    gcagcCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa     >  1:1355011/1‑93 (MQ=255)

                                                                                     cagcCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAt    <  1:2568322/93‑1 (MQ=255)

                                                                                     cagcCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAt    <  1:2102368/93‑1 (MQ=255)

                                                                                      agcCCACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATg   >  2:1777426/1‑93 (MQ=255)

CACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGC  >  NC_000913/217482‑217661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`