BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,910,376	T→C	E75E (GAA→GAG)	htpX ←	heat shock protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,910,376	0	T	C	99.5%	625.8 / ‑5.4	200	E75E (GAA→GAG)	htpX	heat shock protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (90/109); total (91/109)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 205 total aligned reads displayed.

GTTGATGTCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGTTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGAAACGAAGGAACC  >  NC_000913/1910286‑1910465

gTTGATGTCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTc                                                                                         >  1:4876098/1‑93 (MQ=255)

 ttGATGTCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGTTcc                                                                                        >  1:2645165/1‑93 (MQ=255)

 ttGATGTCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTcc                                                                                        <  1:816978/93‑1 (MQ=255)

  tGATGTCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTccc                                                                                       <  2:3692912/93‑1 (MQ=255)

   gATGTCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCt                                                                                      <  1:628243/93‑1 (MQ=255)

     tGTCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCttt                                                                                    >  1:1759758/1‑93 (MQ=255)

      ttCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTc                                                                                   <  2:549820/92‑1 (MQ=255)

      gTCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTc                                                                                   <  1:1689416/93‑1 (MQ=255)

      gTCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTc                                                                                   >  1:558952/1‑93 (MQ=255)

       tCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCg                                                                                  >  2:5068716/1‑93 (MQ=255)

        ccGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGt                                                                                 <  1:924451/93‑1 (MQ=255)

          ggCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTa                                                                               <  1:5319232/93‑1 (MQ=255)

          ggCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTa                                                                               <  1:5155698/93‑1 (MQ=255)

          ggCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTa                                                                               >  1:2390259/1‑93 (MQ=255)

           gcgcATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTAc                                                                              >  1:1601671/1‑93 (MQ=255)

           gcgcATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTAc                                                                              <  1:5510005/93‑1 (MQ=255)

           gcgcATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTAc                                                                              <  1:3548984/93‑1 (MQ=255)

           gcgcATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTAc                                                                              <  2:4701312/93‑1 (MQ=255)

            cgcATGGTAGATAGCCACTTGCGGCATAGCGACCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTAcg                                                                             <  2:2385670/93‑1 (MQ=255)

             gcATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTAcgc                                                                            <  1:4463760/93‑1 (MQ=255)

               aTGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCgg                                                                          >  1:3631553/1‑93 (MQ=255)

                     gATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTc                                                                    <  1:4962166/93‑1 (MQ=255)

                     gATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTc                                                                    >  2:3792702/1‑93 (MQ=255)

                      aTAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCg                                                                   <  1:5746726/93‑1 (MQ=255)

                      aTAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCg                                                                   <  2:289749/93‑1 (MQ=255)

                       tAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGa                                                                  <  1:6093177/93‑1 (MQ=255)

                        aGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGGTGCTCGAt                                                                 >  2:3270913/1‑93 (MQ=255)

                         gCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATc                                                                >  1:3880268/1‑93 (MQ=255)

                         gCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATc                                                                >  2:3635198/1‑93 (MQ=255)

                           cACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCAc                                                              >  2:5405797/1‑93 (MQ=255)

                           cACTTGCGGCATAGCGACCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCAc                                                              <  1:1659876/93‑1 (MQ=255)

                            aCTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACt                                                             >  2:2568763/1‑93 (MQ=255)

                            aCTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACt                                                             >  1:1567143/1‑93 (MQ=255)

                             cTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACtt                                                            >  1:5019982/1‑93 (MQ=255)

                             cTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACtc                                                            >  1:1258374/1‑92 (MQ=255)

                                gCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTccc                                                         <  1:3393743/93‑1 (MQ=255)

                                  ggCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCg                                                       >  2:1195213/1‑93 (MQ=255)

                                  ggCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCg                                                       >  1:4102154/1‑93 (MQ=255)

                                   gCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGc                                                      >  1:4268063/1‑93 (MQ=255)

                                      tAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCaa                                                   >  2:1796964/1‑93 (MQ=255)

                                      tAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCaa                                                   >  1:4531467/1‑93 (MQ=255)

                                        gCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACa                                                 >  1:1354158/1‑93 (MQ=255)

                                        gCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACa                                                 <  1:1198199/93‑1 (MQ=255)

                                        gCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACa                                                 <  2:3987685/93‑1 (MQ=255)

                                         cGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAg                                                <  2:306371/93‑1 (MQ=255)

                                          gagccccGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGa                                               <  1:1472662/90‑1 (MQ=255)

                                          gATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGa                                               >  1:5828609/1‑93 (MQ=255)

                                          gATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGa                                               <  1:2843759/93‑1 (MQ=255)

                                          gATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGa                                               >  1:1867680/1‑93 (MQ=255)

                                           aTCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGAt                                              <  1:5131310/93‑1 (MQ=255)

                                            tCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATc                                             <  2:2429307/93‑1 (MQ=255)

                                            tCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATc                                             <  2:3783797/93‑1 (MQ=255)

                                            tCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATc                                             <  1:2056887/93‑1 (MQ=255)

                                            tCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATc                                             >  1:4772545/1‑93 (MQ=255)

                                            tCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATc                                             >  1:2431346/1‑93 (MQ=255)

                                              cccGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGt                                           >  2:4289617/1‑93 (MQ=255)

                                               ccGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTa                                          >  1:3492891/1‑93 (MQ=255)

                                                cGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTaa                                         <  1:4188105/93‑1 (MQ=255)

                                                 gCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAAt                                        <  1:5895772/93‑1 (MQ=255)

                                                 gCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAAt                                        <  2:810992/93‑1 (MQ=255)

                                                 gCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAAt                                        <  1:6082863/93‑1 (MQ=255)

                                                   cTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGc                                      >  1:963208/1‑93 (MQ=255)

                                                   cTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGc                                      <  2:5871681/93‑1 (MQ=255)

                                                      aCGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGccat                                   >  1:4704696/1‑93 (MQ=255)

                                                       cGAGCCTGGGTTGCTACAGTATTGACCAGACAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGccatc                                  >  2:858700/1‑93 (MQ=255)

                                                        gAGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGccatcc                                 <  2:657818/93‑1 (MQ=255)

                                                         aGCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGccatcca                                >  1:2971199/1‑93 (MQ=255)

                                                          gCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGccatccat                               <  2:21395/93‑1 (MQ=255)

                                                          gCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGccatccat                               <  1:1134386/93‑1 (MQ=255)

                                                          gCCTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGccatccat                               <  1:2315778/93‑1 (MQ=255)

                                                            cTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCAttt                             >  1:1458669/1‑93 (MQ=255)

                                                            cTGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCAttt                             >  1:5355017/1‑93 (MQ=255)

                                                             tGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTg                            >  2:4565776/1‑93 (MQ=255)

                                                             tGGGTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTg                            >  1:3987978/1‑93 (MQ=255)

                                                              gggTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTgg                           >  1:4787727/1‑93 (MQ=255)

                                                               ggTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGa                          <  1:1021277/93‑1 (MQ=255)

                                                               ggTTGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGa                          >  2:3182220/1‑93 (MQ=255)

                                                                  tGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACAt                       >  2:3452449/1‑93 (MQ=255)

                                                                  tGCTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACAt                       <  2:489092/93‑1 (MQ=255)

                                                                   gcaaCAGTATTGACCGGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATc                      <  2:6184707/90‑1 (MQ=255)

                                                                    cTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCa                     <  1:70407/93‑1 (MQ=255)

                                                                    cTACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCa                     <  1:6158710/93‑1 (MQ=255)

                                                                     tACAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAg                    <  1:2200602/93‑1 (MQ=255)

                                                                       cAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGaa                  <  2:2641090/93‑1 (MQ=255)

                                                                       cAGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGaa                  <  2:5716039/93‑1 (MQ=255)

                                                                        aGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAg                 >  2:2083210/1‑93 (MQ=255)

                                                                        aGTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAg                 <  2:5159824/93‑1 (MQ=255)

                                                                         gTATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGc                <  2:4920356/93‑1 (MQ=255)

                                                                          tATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCg               >  2:4982585/1‑93 (MQ=255)

                                                                          tATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCg               >  2:1950324/1‑93 (MQ=255)

                                                                          tATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCg               <  2:4925865/93‑1 (MQ=255)

                                                                          tATTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCg               >  2:4637349/1‑93 (MQ=255)

                                                                           aTTGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGa              >  2:1573712/1‑93 (MQ=255)

                                                                            ttGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCgca             >  2:5426303/1‑91 (MQ=255)

                                                                             tGACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGaaa            <  2:315920/93‑1 (MQ=255)

                                                                              gACCAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCACTTGGACATCAGAAGCGAAAc           >  1:2150025/1‑93 (MQ=255)

                                                                                 cAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGAAACGaa        >  2:1456295/1‑93 (MQ=255)

                                                                                 cAGCCAACGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGAAACGaa        <  2:1144632/93‑1 (MQ=255)

                                                                                      aaCGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGAAACGAAGGAac   >  1:2116506/1‑93 (MQ=255)

                                                                                       aCGCTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGAAACGAAGGAacc  >  1:5396960/1‑93 (MQ=255)

GTTGATGTCCGGCGCATGGTAGATAGCCACTTGCGGCATAGCGATCCCCGCCTGACGAGCCTGGGTTGCTACAGTATTGACCAGCCAACGTTCCCTTTCGTTACGCGGTTGCTCGATCACTTCCCCGCCAACAGATCGTAATGCCATCCATTTGGACATCAGAAGCGAAACGAAGGAACC  >  NC_000913/1910286‑1910465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`