BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	219,906	A→G	T30T (ACT→ACC)	rcsF ←	regulator in colanic acid synthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	219,906	0	A	G	100.0%	682.6 / NA	216	T30T (ACT→ACC)	rcsF	regulator in colanic acid synthesis
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (94/122); total (94/122)

Only 100 of 246 total aligned reads displayed.

GCCGACTAATTCTTCTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCAT  >  NC_000913/219816‑219995

gCCGACTAATTCTTCTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGt                                                                                         >  2:2835089/1‑93 (MQ=255)

 ccGACTAATTCTTCTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTg                                                                                        >  1:6140619/1‑93 (MQ=255)

  cGACTAATTCTTCTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGGGCGGTGc                                                                                       >  1:56835/1‑93 (MQ=255)

   gACTAATTCTTCTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCt                                                                                      >  2:6292707/1‑93 (MQ=255)

   gACTAATTCTTCTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCt                                                                                      >  2:3991407/1‑93 (MQ=255)

    aCTAATTCTTCTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCtt                                                                                     >  2:2149913/1‑93 (MQ=255)

      tAATTCTTCTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTg                                                                                   >  1:425055/1‑93 (MQ=255)

      tAATTCTTCTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGCGCGGTGCTTTg                                                                                   >  2:1011155/1‑93 (MQ=255)

       aaTTCTTCTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGGGCGGTGGGTTGa                                                                                  >  2:4363096/1‑93 (MQ=255)

         ttcttcTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGGGGGGTGCTTTGAAc                                                                                >  1:20398/1‑93 (MQ=255)

         ttcttcTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGCGCGGTGGTTTGAAc                                                                                >  1:279965/1‑93 (MQ=255)

          tcttctGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACg                                                                               <  2:67731/93‑1 (MQ=255)

          tcttctGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACg                                                                               <  2:2517129/93‑1 (MQ=255)

            ttctGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGGACggg                                                                             >  1:502272/1‑93 (MQ=255)

            ttctGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACggg                                                                             >  1:3950841/1‑93 (MQ=255)

            ttctGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACggg                                                                             <  2:374251/93‑1 (MQ=255)

             tctGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAATGGGt                                                                            >  2:4487691/1‑93 (MQ=255)

             tctGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGt                                                                            >  1:117375/1‑93 (MQ=255)

              ctGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGtt                                                                           <  1:3099902/93‑1 (MQ=255)

              ctGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAAGCTCCGCTTTCGGCTGGGGCGCGGTGCTTTGTACGGGtt                                                                           >  1:1016450/1‑93 (MQ=255)

               tGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTc                                                                          >  1:5774956/1‑93 (MQ=255)

                 cATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGa                                                                        <  1:25861/93‑1 (MQ=255)

                  aTTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGAc                                                                       >  2:504986/1‑93 (MQ=255)

                  aTTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGAc                                                                       >  1:397680/1‑93 (MQ=255)

                  aTTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGGGCGGTGCTTTGAACGGGTTCGAc                                                                       >  2:1707230/1‑93 (MQ=255)

                   ttGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGGACGGGGTCGACa                                                                      >  1:3332657/1‑93 (MQ=255)

                   ttGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACa                                                                      >  2:2887984/1‑93 (MQ=255)

                    tGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAg                                                                     >  2:5273356/1‑93 (MQ=255)

                    tGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAg                                                                     >  2:622698/1‑93 (MQ=255)

                     ggTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAgg                                                                    <  2:764894/93‑1 (MQ=255)

                     ggTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAgg                                                                    >  1:4650650/1‑93 (MQ=255)

                        ataAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAggggg                                                                 >  1:4126246/1‑92 (MQ=255)

                        ataAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGGTCGACAGGGGa                                                                 >  2:2938487/1‑93 (MQ=255)

                           aaTTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCt                                                              >  2:3058098/1‑93 (MQ=255)

                           aaTTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCt                                                              <  2:3582273/93‑1 (MQ=255)

                           aaTTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGGTCCACAGGGGATCt                                                              >  2:2631088/1‑93 (MQ=255)

                            aTTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTg                                                             >  2:4783718/1‑93 (MQ=255)

                                ggACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTa                                                         <  2:2350453/93‑1 (MQ=255)

                                   ccGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACa                                                      <  1:2011218/93‑1 (MQ=255)

                                   ccGGCGTGGCGCGCGGCGCTTTCGGGTTTGCAGGGTCCGCCTTCGGCTGGGGGGGGGGGGTTTTAACGGGGTCGACAGGGGGTCTGCTTTACa                                                      >  2:4298349/1‑93 (MQ=255)

                                    cGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTGACAt                                                     >  2:5768827/1‑93 (MQ=255)

                                    cGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACAt                                                     <  2:589557/93‑1 (MQ=255)

                                    cGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACAt                                                     <  1:1323842/93‑1 (MQ=255)

                                     ggCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATg                                                    >  2:1892967/1‑93 (MQ=255)

                                     ggCGTGGCGCGCGGCGCTTTCGGGTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGGTCGGCAGGGGAACTGCTTAACATg                                                    >  2:3884107/1‑93 (MQ=255)

                                      gCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATgg                                                   <  2:4554982/93‑1 (MQ=255)

                                      gCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATgg                                                   >  1:481006/1‑93 (MQ=255)

                                      gCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATgg                                                   <  2:6170072/93‑1 (MQ=255)

                                      gCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGCGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATgg                                                   >  1:1779976/1‑93 (MQ=255)

                                       cGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGa                                                  >  1:726080/1‑93 (MQ=255)

                                        gTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGaa                                                 >  2:933316/1‑93 (MQ=255)

                                         tGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAAc                                                <  2:5159405/93‑1 (MQ=255)

                                          ggCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATTGAACa                                               <  2:1276878/93‑1 (MQ=255)

                                          ggCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACa                                               >  1:1456211/1‑93 (MQ=255)

                                           gcgcgcGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAg                                              <  2:5565531/93‑1 (MQ=255)

                                             gcgcgGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGcc                                            <  2:724506/93‑1 (MQ=255)

                                             gcgcgGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGcc                                            <  2:1611943/93‑1 (MQ=255)

                                               gcggcgCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGAGGCTCTGCTTAACATGCAACAGcagt                                          >  2:4282673/1‑90 (MQ=255)

                                                cggcgCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCt                                         >  2:2104647/1‑93 (MQ=255)

                                                 ggcgCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCtt                                        >  1:5544674/1‑93 (MQ=255)

                                                  gcgcTTTCGGTTTTGCAGTCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTa                                       <  2:108508/93‑1 (MQ=255)

                                                   cgcTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAg                                      <  1:2306642/93‑1 (MQ=255)

                                                    gcTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGTGGATCTGCTTAACATGGAACAGCCGCGTAGc                                     >  2:5376797/1‑93 (MQ=255)

                                                    gcTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGc                                     <  1:4069117/93‑1 (MQ=255)

                                                    gcTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCCTAACATGGAACAGCCGCTTAgt                                     >  2:1512774/1‑92 (MQ=255)

                                                     cTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCa                                    <  1:4517760/93‑1 (MQ=255)

                                                     cTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCa                                    <  2:180260/93‑1 (MQ=255)

                                                      tttCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCAt                                   >  2:2244891/1‑93 (MQ=255)

                                                      tttCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCAt                                   >  2:495428/1‑93 (MQ=255)

                                                        tCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGa                                 >  2:3907251/1‑93 (MQ=255)

                                                         cGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAg                                <  2:1567904/93‑1 (MQ=255)

                                                          ggTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGt                               <  2:787355/93‑1 (MQ=255)

                                                            ttttGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGc                             <  2:5526583/93‑1 (MQ=255)

                                                            ttttGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGc                             <  2:4146626/93‑1 (MQ=255)

                                                              ttGCAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGcta                           <  1:6120250/93‑1 (MQ=255)

                                                                 cAGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGctacta                        >  1:3250756/1‑93 (MQ=255)

                                                                  aGGCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGctactca                       >  1:5403696/1‑91 (MQ=255)

                                                                    gCTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAAc                     <  1:3557982/93‑1 (MQ=255)

                                                                     cTCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACa                    <  2:571718/93‑1 (MQ=255)

                                                                      tCCGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAg                   >  2:5673932/1‑93 (MQ=255)

                                                                       ccGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGa                  <  2:3686528/93‑1 (MQ=255)

                                                                        cGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGAt                 <  1:2677197/93‑1 (MQ=255)

                                                                        cGCTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGAt                 <  1:5244304/93‑1 (MQ=255)

                                                                          cTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCg               >  1:2613479/1‑93 (MQ=255)

                                                                          cTTTCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCg               <  1:1844427/93‑1 (MQ=255)

                                                                           tttCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGCACAGCCGCTTAGCCTGAGTGCTACTAAACAGATCgg              >  2:3956061/1‑93 (MQ=255)

                                                                           tttCGGCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCgg              >  2:2438271/1‑93 (MQ=255)

                                                                                gCTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAg         >  2:3324919/1‑93 (MQ=255)

                                                                                gCTGGGGTGCGGTGCTTTGAACGGGGTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAg         >  1:3508477/1‑93 (MQ=255)

                                                                                 cTGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGc        <  1:870713/93‑1 (MQ=255)

                                                                                  tGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCa       <  2:4991297/93‑1 (MQ=255)

                                                                                  tGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCa       <  1:2933097/93‑1 (MQ=255)

                                                                                  tGGGGTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCa       >  1:4694729/1‑93 (MQ=255)

                                                                                   ggggTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCAc      >  1:3809016/1‑93 (MQ=255)

                                                                                    gggTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACg     <  1:1560997/93‑1 (MQ=255)

                                                                                      gTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCa   <  2:5488134/93‑1 (MQ=255)

                                                                                      gTGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCa   <  1:1469945/93‑1 (MQ=255)

                                                                                       tGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCAt  <  1:33763/93‑1 (MQ=255)

                                                                                       tGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCAt  <  1:1652322/93‑1 (MQ=255)

                                                                                       tGCGGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCAt  <  1:2181734/93‑1 (MQ=255)

GCCGACTAATTCTTCTGCATTGGTATAAATTCGGACCGGCGTGGCGCGCGGCGCTTTCGGTTTTGCAGGCTCCGCTTTCGGCTGGGGTGCAGTGCTTTGAACGGGTTCGACAGGGGATCTGCTTAACATGGAACAGCCGCTTAGCATGAGTGCTACTAAACAGATCGGTAAAGCACGCAT  >  NC_000913/219816‑219995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`