BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	232,218	A→G	D98G (GAC→GGC)	yafE →	putative biotin synthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	232,218	0	A	G	100.0%	768.4 / NA	240	D98G (GAC→GGC)	yafE	putative biotin synthesis protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (123/117); total (123/117)

Only 100 of 245 total aligned reads displayed.

GTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCA  >  NC_000913/232128‑232307

gTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCg                                                                                         >  1:3114603/1‑93 (MQ=255)

gTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCg                                                                                         >  2:3057169/1‑93 (MQ=255)

 ttATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGt                                                                                        >  1:4678670/1‑93 (MQ=255)

 ttATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGt                                                                                        <  1:6301680/93‑1 (MQ=255)

 ttATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGt                                                                                        <  1:4929422/93‑1 (MQ=255)

  tATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTa                                                                                       >  1:6175969/1‑93 (MQ=255)

  tATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTa                                                                                       <  1:4865925/93‑1 (MQ=255)

   aTTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTaa                                                                                      >  2:3618039/1‑93 (MQ=255)

   aTTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTaa                                                                                      >  1:2877497/1‑93 (MQ=255)

   aTTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTaa                                                                                      <  2:2286595/93‑1 (MQ=255)

    ttCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAAt                                                                                     <  2:1599607/93‑1 (MQ=255)

     tCTGCACATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATg                                                                                    <  2:1311433/93‑1 (MQ=255)

      cTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGt                                                                                   >  2:4572847/1‑93 (MQ=255)

       tGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGtc                                                                                  >  1:1418453/1‑93 (MQ=255)

           cATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCg                                                                              <  2:1011582/93‑1 (MQ=255)

           cATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCg                                                                              >  1:245183/1‑93 (MQ=255)

           cATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCg                                                                              >  2:3022086/1‑93 (MQ=255)

            aTCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCgg                                                                             <  2:847347/93‑1 (MQ=255)

            aTCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCgg                                                                             <  1:4013797/93‑1 (MQ=255)

            aTCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCgg                                                                             <  2:5702931/93‑1 (MQ=255)

             tCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCggg                                                                            <  1:3140498/93‑1 (MQ=255)

             tCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCggg                                                                            <  2:2966898/93‑1 (MQ=255)

             tCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCggg                                                                            <  2:2169033/93‑1 (MQ=255)

             tCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGCTAGGCTGATTGTGATGGGCGTAATGTCTCCggg                                                                            >  2:3212981/1‑93 (MQ=255)

              cACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGt                                                                           >  2:5411287/1‑93 (MQ=255)

               aCTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTc                                                                          >  1:6146467/1‑93 (MQ=255)

               aCTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTc                                                                          >  1:45233/1‑93 (MQ=255)

                 tGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCAc                                                                        >  2:5090915/1‑93 (MQ=255)

                  ggCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCAcc                                                                       <  2:2072018/93‑1 (MQ=255)

                  ggCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCAcc                                                                       <  1:61078/93‑1 (MQ=255)

                    cATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCa                                                                     <  1:68606/93‑1 (MQ=255)

                        atgTTGGGGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTgc                                                                 >  1:5908767/1‑93 (MQ=255)

                         tgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTgcg                                                                >  1:1771426/1‑93 (MQ=255)

                           ttGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGcgcg                                                              >  2:395678/1‑93 (MQ=255)

                             ggTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCGCCCAGTGCGCGAc                                                            <  2:1719292/93‑1 (MQ=255)

                             ggTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGcgcgcc                                                            >  2:5637710/1‑91 (MQ=255)

                             ggTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGAc                                                            >  1:4734472/1‑93 (MQ=255)

                              gTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACa                                                           >  1:1031119/1‑93 (MQ=255)

                                gcagcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGAGCGACATc                                                         <  1:5087919/93‑1 (MQ=255)

                                 cagcaCTGCGAGAAGTGAATAGGATATTGAAGCCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGAGACATCt                                                        <  2:6195941/93‑1 (MQ=255)

                                  agcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTg                                                       <  1:2347213/93‑1 (MQ=255)

                                   gcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTgg                                                      >  2:1540949/1‑93 (MQ=255)

                                      cTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTa                                                   <  2:521002/93‑1 (MQ=255)

                                      cTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTa                                                   >  2:4679216/1‑93 (MQ=255)

                                      cTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGAGATGGGCGTAATGTCTCCGGGTAACCCAGTGCGCGACATCTGGTTa                                                   <  2:5073535/93‑1 (MQ=255)

                                        gCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACa                                                 >  1:5720311/1‑93 (MQ=255)

                                          gagaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGa                                               >  1:541007/1‑93 (MQ=255)

                                          gagaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGa                                               <  1:1557952/93‑1 (MQ=255)

                                          gagaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGa                                               <  2:657286/93‑1 (MQ=255)

                                          gagaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGa                                               >  1:4350510/1‑93 (MQ=255)

                                           agaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGAc                                              <  1:2660454/93‑1 (MQ=255)

                                           agaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGAc                                              >  2:6254897/1‑93 (MQ=255)

                                            gaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACg                                             >  1:784177/1‑93 (MQ=255)

                                             aaGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACgg                                            >  1:1196785/1‑93 (MQ=255)

                                             aaGTGAATAGAATATTGAAA‑CTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGt                                           <  1:5049808/93‑1 (MQ=255)

                                              aGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGt                                           <  1:304227/93‑1 (MQ=255)

                                              aGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGt                                           >  2:2843997/1‑93 (MQ=255)

                                              aGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGt                                           >  1:3907838/1‑93 (MQ=255)

                                               gTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTa                                          <  2:731971/93‑1 (MQ=255)

                                               gTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTa                                          <  1:5638377/93‑1 (MQ=255)

                                                tGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGTGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAg                                         >  2:3927602/1‑93 (MQ=255)

                                                tGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAg                                         >  1:2232855/1‑93 (MQ=255)

                                                 gAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                                        >  2:2212918/1‑93 (MQ=255)

                                                 gAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                                        <  1:3197214/93‑1 (MQ=255)

                                                  aaTAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGaa                                       >  1:1392909/1‑93 (MQ=255)

                                                    tAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGc                                     >  1:2290601/1‑93 (MQ=255)

                                                     aGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCa                                    <  1:2828750/93‑1 (MQ=255)

                                                      ggATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAt                                   <  1:2971140/93‑1 (MQ=255)

                                                      ggATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAt                                   >  2:351859/1‑93 (MQ=255)

                                                      ggATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAt                                   >  1:2128313/1‑93 (MQ=255)

                                                       gATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAtt                                  >  2:805327/1‑93 (MQ=255)

                                                        atatTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTa                                 <  1:263598/93‑1 (MQ=255)

                                                        atatTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTa                                 <  1:5798994/93‑1 (MQ=255)

                                                         tatTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAc                                <  1:3528221/93‑1 (MQ=255)

                                                         tatTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAc                                >  2:4259340/1‑93 (MQ=255)

                                                         tatTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAc                                >  2:4901187/1‑93 (MQ=255)

                                                         tatTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAc                                >  1:4256457/1‑93 (MQ=255)

                                                           ttGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcgc                              <  2:5668554/93‑1 (MQ=255)

                                                             gAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGa                            <  1:1057701/93‑1 (MQ=255)

                                                              aaaCCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGAt                           <  1:5696497/93‑1 (MQ=255)

                                                               aaCCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATa                          >  1:1878334/1‑93 (MQ=255)

                                                                 ccTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATAcc                        <  2:3805661/93‑1 (MQ=255)

                                                                  cTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACct                       >  2:4370433/1‑93 (MQ=255)

                                                                   tGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctc                      <  2:3833756/93‑1 (MQ=255)

                                                                      gggTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCa                   <  1:4190801/92‑1 (MQ=255)

                                                                         tAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGt                >  1:3586256/1‑93 (MQ=255)

                                                                           ggCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTAc              >  2:401593/1‑93 (MQ=255)

                                                                           ggCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTAc              >  1:3872555/1‑93 (MQ=255)

                                                                            gCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACg             >  2:1178828/1‑93 (MQ=255)

                                                                            gCTGATGGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTaag             <  2:6029224/93‑3 (MQ=255)

                                                                             cTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGa            <  1:5305136/93‑1 (MQ=255)

                                                                               gATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaaa          >  2:3464182/1‑93 (MQ=255)

                                                                                aTTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAAc         <  2:4853766/93‑1 (MQ=255)

                                                                                aTTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAAc         <  2:1391155/93‑1 (MQ=255)

                                                                                aTTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAAc         >  2:5560737/1‑93 (MQ=255)

                                                                                aTTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAAc         <  1:1496610/93‑1 (MQ=255)

                                                                                aTTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAAc         <  1:3852474/93‑1 (MQ=255)

                                                                                aTTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAAc         >  2:2507844/1‑93 (MQ=255)

                                                                                aTTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAAc         <  1:4319672/93‑1 (MQ=255)

                                                                                 ttGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACt        >  2:3329090/1‑93 (MQ=255)

GTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCA  >  NC_000913/232128‑232307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`