BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,089,888	T→C	L257P (CTC→CCC)	hisD →	L‑histidinal:NAD+ oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,089,888	0	T	C	100.0%	591.3 / NA	182	L257P (CTC→CCC)	hisD	L‑histidinal:NAD+ oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (86/96); total (86/96)

Only 100 of 189 total aligned reads displayed.

TGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAC  >  NC_000913/2089799‑2089977

tGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGccctc                                                                                        <  1:817253/93‑1 (MQ=255)

 gcggcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGccctct                                                                                       >  1:5955710/1‑93 (MQ=255)

  cggcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCcctctc                                                                                      <  1:3086046/93‑1 (MQ=255)

    gcgATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAg                                                                                    <  2:5095867/93‑1 (MQ=255)

      gatcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGc                                                                                  >  1:4247646/1‑93 (MQ=255)

      gatcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGc                                                                                  >  1:5630845/1‑93 (MQ=255)

       atcgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCt                                                                                 >  1:3074609/1‑93 (MQ=255)

         cgatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGa                                                                               <  2:2322441/93‑1 (MQ=255)

          gatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGaa                                                                              <  2:6296780/93‑1 (MQ=255)

          gatATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGaa                                                                              >  1:3991095/1‑93 (MQ=255)

           atatGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAac                                                                             >  2:1627505/1‑93 (MQ=255)

             atGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAacac                                                                           >  2:1496716/1‑93 (MQ=255)

              tGCCCGCAGGCCCGTCGGAAGTGCTGGTAATTGCTGACAGCGGCGCTAAGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACg                                                                          <  1:6305256/93‑1 (MQ=255)

                 ccGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGcc                                                                       >  1:2726914/1‑93 (MQ=255)

                 ccGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGcc                                                                       <  2:2373289/93‑1 (MQ=255)

                  cGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGccc                                                                      >  2:1148032/1‑93 (MQ=255)

                  cGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGccc                                                                      >  2:1897577/1‑93 (MQ=255)

                     aGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACCCGGCCCGGa                                                                   >  1:2055653/1‑93 (MQ=255)

                     aGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGa                                                                   >  2:4344790/1‑93 (MQ=255)

                        cccGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTc                                                                >  2:5153164/1‑93 (MQ=255)

                         ccGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTca                                                               <  1:6222347/93‑1 (MQ=255)

                         ccGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTca                                                               >  1:1268761/1‑93 (MQ=255)

                          cGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTcac                                                              >  2:5756201/1‑93 (MQ=255)

                          cGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTcac                                                              >  2:842697/1‑93 (MQ=255)

                           gTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTcaca                                                             >  2:4130063/1‑93 (MQ=255)

                           gTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTcaca                                                             >  1:730385/1‑93 (MQ=255)

                            tCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAg                                                            <  2:2558467/93‑1 (MQ=255)

                            gcGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAg                                                            <  1:1767668/92‑1 (MQ=255)

                             cGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAgg                                                           <  2:2943553/93‑1 (MQ=255)

                              ggAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAggg                                                          >  2:3870578/1‑92 (MQ=255)

                               gAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTg                                                         >  2:6075328/1‑93 (MQ=255)

                                aaGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGa                                                        <  2:4063806/93‑1 (MQ=255)

                                 aGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGGCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAt                                                       <  1:2893056/93‑1 (MQ=255)

                                 aGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAt                                                       >  1:5774770/1‑93 (MQ=255)

                                  gTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAtt                                                      <  1:1832732/93‑1 (MQ=255)

                                   tGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAttt                                                     >  2:3791244/1‑93 (MQ=255)

                                   ggCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGAttt                                                     <  1:4853817/92‑1 (MQ=255)

                                      tGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTAc                                                  >  2:3879741/1‑93 (MQ=255)

                                       ggTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACt                                                 >  1:2499147/1‑93 (MQ=255)

                                        gTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTg                                                >  1:5265888/1‑93 (MQ=255)

                                         tGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGa                                               <  2:4251273/93‑1 (MQ=255)

                                          gATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGAc                                              >  2:5112880/1‑93 (MQ=255)

                                          gATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGAc                                              <  1:1035816/93‑1 (MQ=255)

                                          gATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGAc                                              <  1:3272090/93‑1 (MQ=255)

                                           aTTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGAcc                                             >  1:7773/1‑92 (MQ=255)

                                           aTTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACg                                             >  1:3014552/1‑93 (MQ=255)

                                            ttGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGc                                            <  1:906828/93‑1 (MQ=255)

                                              gCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGccc                                          <  2:3082354/93‑1 (MQ=255)

                                              gCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGccc                                          <  1:4110917/93‑1 (MQ=255)

                                                tGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgc                                        >  2:2805550/1‑93 (MQ=255)

                                                tGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgc                                        <  2:648104/93‑1 (MQ=255)

                                                 gACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgct                                       >  1:1148072/1‑93 (MQ=255)

                                                  aCAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgctg                                      >  1:140423/1‑93 (MQ=255)

                                                   cAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgctgc                                     >  1:2281453/1‑93 (MQ=255)

                                                   cAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgctgc                                     <  2:2949199/93‑1 (MQ=255)

                                                    aGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCgctgct                                    >  1:4291301/1‑93 (MQ=255)

                                                     gcggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGctgctg                                   >  2:2276519/1‑93 (MQ=255)

                                                     gcggcgCTACGCCGGATTTAGTGGCTTCTGATTTGGCCTCTCAGGCTGAACACGGCCCGGACTCACAGGCGATTTTACTGACGCCcgccgccg                                   >  2:5315778/1‑91 (MQ=255)

                                                      cggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGa                                  <  1:3336357/93‑1 (MQ=255)

                                                      cggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGa                                  <  1:3711425/93‑1 (MQ=255)

                                                      cggcgCTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGa                                  <  1:3560894/93‑1 (MQ=255)

                                                        gcgcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGata                                <  1:3458968/93‑1 (MQ=255)

                                                         cgcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGatat                               <  1:1681641/93‑1 (MQ=255)

                                                          gcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATg                              >  1:5219739/1‑93 (MQ=255)

                                                          gcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATg                              >  2:2185002/1‑93 (MQ=255)

                                                          gcTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATg                              <  1:1575538/93‑1 (MQ=255)

                                                           cTACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATgg                             <  1:3360474/93‑1 (MQ=255)

                                                            tACGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGgc                            <  1:2599152/93‑1 (MQ=255)

                                                             aCGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGgcg                           <  1:5494341/93‑1 (MQ=255)

                                                             aCGCCGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCCGATATGgcg                           >  2:3551516/1‑93 (MQ=255)

                                                                ccGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgt                        <  2:3612438/93‑1 (MQ=255)

                                                                ccGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgt                        <  1:3205664/93‑1 (MQ=255)

                                                                 cGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtc                       <  2:2593999/93‑1 (MQ=255)

                                                                 cGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtc                       >  2:5635449/1‑93 (MQ=255)

                                                                 cGGATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtc                       >  2:4733538/1‑93 (MQ=255)

                                                                  ggATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcg                      <  1:3194597/93‑1 (MQ=255)

                                                                   gATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgc                     >  2:3441416/1‑93 (MQ=255)

                                                                   gATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgc                     >  1:192424/1‑93 (MQ=255)

                                                                   gATTTCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGGGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgc                     >  1:182010/1‑93 (MQ=255)

                                                                     tttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTTACGCCCGCTGCTGATATGGcgcgtcgcgt                   >  1:2451643/1‑93 (MQ=255)

                                                                     tttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgcgt                   >  2:1117382/1‑93 (MQ=255)

                                                                     tttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgcgt                   <  1:2961038/93‑1 (MQ=255)

                                                                     tttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGcgcgtcgcgt                   >  1:3310648/1‑93 (MQ=255)

                                                                      ttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGtt                  <  1:3547789/93‑1 (MQ=255)

                                                                      ttCGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGtt                  <  1:5305848/93‑1 (MQ=255)

                                                                        cGTGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGc                <  2:2711543/93‑1 (MQ=255)

                                                                          tGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCg              >  2:3832717/1‑93 (MQ=255)

                                                                          tGGCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCg              >  2:4175570/1‑93 (MQ=255)

                                                                           ggCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGa             >  1:4398471/1‑93 (MQ=255)

                                                                           ggCTTCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGa             <  2:2975722/93‑1 (MQ=255)

                                                                              ttCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGc          >  1:3627908/1‑93 (MQ=255)

                                                                               tCTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGcc         <  1:5051158/93‑1 (MQ=255)

                                                                                cTGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCg        <  1:1455327/93‑1 (MQ=255)

                                                                                 tGATTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGt       <  2:2150389/93‑1 (MQ=255)

                                                                                   aTTTGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCg     <  1:4611957/93‑1 (MQ=255)

                                                                                    tttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGa    >  1:3332576/1‑93 (MQ=255)

                                                                                    tttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGa    >  1:1216910/1‑93 (MQ=255)

                                                                                     ttGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGaa   <  2:1451777/93‑1 (MQ=255)

                                                                                      tGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAc  >  1:4722283/1‑93 (MQ=255)

                                                                                      tGCCCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAc  <  2:4414733/93‑1 (MQ=255)

TGCGGCGATCGATATGCCCGCAGGCCCGTCGGAAGTGCTGGTGATTGCTGACAGCGGCGCTACGCCGGATTTCGTGGCTTCTGATTTGCTCTCTCAGGCTGAACACGGCCCGGACTCACAGGTGATTTTACTGACGCCCGCTGCTGATATGGCGCGTCGCGTTGCCGAGGCCGTCGAAC  >  NC_000913/2089799‑2089977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`