BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,094,539	T→C	V225A (GTT→GCT)	hisF →	imidazole glycerol phosphate synthase holoenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,094,539	0	T	C	100.0%	683.8 / NA	213	V225A (GTT→GCT)	hisF	imidazole glycerol phosphate synthase holoenzyme
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (98/115); total (98/115)

Only 100 of 223 total aligned reads displayed.

AAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGA  >  NC_000913/2094449‑2094628

 aGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGa                                                                                        <  1:4305620/93‑1 (MQ=255)

   tGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACg                                                                                      >  1:850527/1‑93 (MQ=255)

    gCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACgg                                                                                     <  2:1160032/93‑1 (MQ=255)

    gCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACgg                                                                                     <  1:1014140/93‑1 (MQ=255)

    gCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCAGCGATGCCGACGCTGACgg                                                                                     <  2:4316028/93‑1 (MQ=255)

     cGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgc                                                                                    >  2:5333271/1‑93 (MQ=255)

      gTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcg                                                                                   >  1:4512622/1‑93 (MQ=255)

      gTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcg                                                                                   >  2:337636/1‑93 (MQ=255)

        gAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcgcg                                                                                 <  1:5739489/93‑1 (MQ=255)

         aaGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGgcgcgc                                                                                <  1:1039576/93‑1 (MQ=255)

          aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               >  2:6181021/1‑93 (MQ=255)

          aGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCt                                                                               <  1:1998955/93‑1 (MQ=255)

           gTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTg                                                                              <  2:396001/93‑1 (MQ=255)

            tttGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTgg                                                                             >  2:629713/1‑93 (MQ=255)

             ttGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGc                                                                            >  1:5924585/1‑93 (MQ=255)

             ttGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGc                                                                            >  1:3041978/1‑93 (MQ=255)

              tGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCa                                                                           <  1:2795942/93‑1 (MQ=255)

               gCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGACGCGCGGGCAg                                                                          >  2:5343260/1‑93 (MQ=255)

                   cGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTc                                                                      <  1:3818713/93‑1 (MQ=255)

                     tCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCg                                                                    >  2:3706587/1‑93 (MQ=255)

                     tCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCg                                                                    >  1:3990712/1‑93 (MQ=255)

                     tCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGAAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCCGGCAGCTTCCg                                                                    >  2:1462806/1‑93 (MQ=255)

                       ccGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTa                                                                  <  1:4843805/93‑1 (MQ=255)

                        cGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 >  2:5049344/1‑93 (MQ=255)

                        cGCTGATGGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAt                                                                 <  2:1951219/93‑1 (MQ=255)

                         gCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTAtt                                                                >  2:3096436/1‑93 (MQ=255)

                           tGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTcc                                                              <  2:1415522/93‑1 (MQ=255)

                           tGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTcc                                                              >  1:2415772/1‑93 (MQ=255)

                            gATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCca                                                             >  2:2174670/1‑93 (MQ=255)

                            gATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCca                                                             <  1:345251/93‑1 (MQ=255)

                               tGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaa                                                          >  2:4728565/1‑93 (MQ=255)

                                gCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaa                                                         >  1:165435/1‑93 (MQ=255)

                                 ccTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaac                                                        >  1:1605632/1‑93 (MQ=255)

                                 ccTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaac                                                        >  1:6151385/1‑93 (MQ=255)

                                 ccTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaac                                                        <  1:5145393/93‑1 (MQ=255)

                                 ccTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaac                                                        >  1:5337169/1‑93 (MQ=255)

                                  cTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaaca                                                       >  1:6256313/1‑93 (MQ=255)

                                   tCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCacaaacaa                                                      >  2:1655522/1‑93 (MQ=255)

                                     cGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACCAACAaat                                                    <  2:5985794/93‑1 (MQ=255)

                                     cGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    <  1:3652392/93‑1 (MQ=255)

                                     cGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaat                                                    <  1:1201936/93‑1 (MQ=255)

                                      ggTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAaata                                                   <  1:4569805/93‑1 (MQ=255)

                                         ggCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATc                                                <  2:1778378/93‑1 (MQ=255)

                                            gcgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAat                                             <  2:1632369/93‑1 (MQ=255)

                                            gcgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAat                                             <  1:2769069/93‑1 (MQ=255)

                                            gcgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAat                                             >  2:624686/1‑93 (MQ=255)

                                             cgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAata                                            <  1:5463805/93‑1 (MQ=255)

                                             cgGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAata                                            <  1:3160699/93‑1 (MQ=255)

                                              gggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAatat                                           >  1:4074199/1‑93 (MQ=255)

                                               ggCACCCTGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATCtt                                          >  2:5241281/1‑93 (MQ=255)

                                               ggCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATAtt                                          <  1:3581383/93‑1 (MQ=255)

                                                gCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTg                                         >  2:485655/1‑93 (MQ=255)

                                                gCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTg                                         <  2:2878156/93‑1 (MQ=255)

                                                  aCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGt                                       >  2:4653978/1‑93 (MQ=255)

                                                  aCCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGt                                       <  1:2715956/93‑1 (MQ=255)

                                                     aTGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGaa                                    <  1:6188675/93‑1 (MQ=255)

                                                      tGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAAt                                   <  1:5661951/93‑1 (MQ=255)

                                                        gAACACTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTa                                 >  2:5888045/1‑93 (MQ=255)

                                                          acacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTaaa                               >  2:5533506/1‑93 (MQ=255)

                                                          acacTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTCGCAGCTTCCGTATTCCACAAACAAATCATCAATATTGGAGAATTaaa                               >  1:1929392/1‑93 (MQ=255)

                                                            acTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAg                             <  2:4993874/93‑1 (MQ=255)

                                                             cTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGc                            <  1:1327698/93‑1 (MQ=255)

                                                             cTTCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGc                            <  1:3117290/93‑1 (MQ=255)

                                                              ttCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCg                           >  1:864482/1‑93 (MQ=255)

                                                              ttCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCg                           >  2:4396844/1‑93 (MQ=255)

                                                              ttCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCg                           <  2:5376757/93‑1 (MQ=255)

                                                               tCCTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGt                          <  1:1335085/93‑1 (MQ=255)

                                                               tCCTCGAAGCCTTCCGCGATGCCGACACTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGt                          >  2:1938414/1‑93 (MQ=255)

                                                                ccTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTa                         <  1:5781159/93‑1 (MQ=255)

                                                                ccTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTa                         <  2:2264677/93‑1 (MQ=255)

                                                                 cTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAc                        <  1:6093017/93‑1 (MQ=255)

                                                                 cTCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAc                        <  1:3334135/93‑1 (MQ=255)

                                                                  tCGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTAcc                       <  2:5176241/93‑1 (MQ=255)

                                                                   cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCt                      >  1:4323313/1‑93 (MQ=255)

                                                                   cGAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCt                      <  1:4803580/93‑1 (MQ=255)

                                                                    gAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTg                     >  1:1705670/1‑93 (MQ=255)

                                                                    gAAGCCTTCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTg                     <  1:3509498/93‑1 (MQ=255)

                                                                      aGCCTACCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATGGGTGAATTAAAAGCGTACCTGGc                   <  2:1240199/93‑1 (MQ=255)

                                                                          ttCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCaaga               >  1:2153974/1‑91 (MQ=255)

                                                                          ttCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAaca               >  2:2402628/1‑93 (MQ=255)

                                                                           tCCGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAtcac              <  2:1999111/93‑1 (MQ=255)

                                                                            ccGCGATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAAcaca             >  2:2571049/1‑93 (MQ=255)

                                                                              gcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAgg           <  2:904965/93‑1 (MQ=255)

                                                                              gcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAgg           >  2:582452/1‑93 (MQ=255)

                                                                              gcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAgg           >  2:4575210/1‑93 (MQ=255)

                                                                              gcgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAgg           <  1:3243416/93‑1 (MQ=255)

                                                                               cgATGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAggg          <  2:5557158/93‑1 (MQ=255)

                                                                                  tGCCGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCgg       >  2:5779556/1‑92 (MQ=255)

                                                                                    ccGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTgg     >  2:3003682/1‑93 (MQ=255)

                                                                                    ccGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTgg     >  2:1152874/1‑93 (MQ=255)

                                                                                    ccGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTgg     >  1:4888913/1‑93 (MQ=255)

                                                                                    ccGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTgg     <  1:2582358/93‑1 (MQ=255)

                                                                                    ccGACGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTgg     <  1:2067493/93‑1 (MQ=255)

                                                                                     cGACGCTGACGGCGCGATGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAATACAGGTCTTGga    <  2:871737/93‑1 (MQ=255)

                                                                                       aCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgaga  >  2:3850037/1‑93 (MQ=255)

                                                                                       aCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgaga  <  2:2833375/93‑1 (MQ=255)

                                                                                       aCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgaga  <  2:5679921/93‑1 (MQ=255)

                                                                                       aCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgaga  <  2:5769672/93‑1 (MQ=255)

                                                                                       aCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgaga  <  2:251842/93‑1 (MQ=255)

                                                                                       aCGCTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGgaga  >  1:5270114/1‑93 (MQ=255)

AAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAACACTTCCTCGAAGCCTTCCGCGATGCCGACGTTGACGGCGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGTGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGA  >  NC_000913/2094449‑2094628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`