BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	245,050	C→T	P242S (CCA→TCA)	yafJ →	putative amidotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	245,050	0	C	T	100.0%	930.0 / NA	250	P242S (CCA→TCA)	yafJ	putative amidotransferase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (127/123); total (127/123)

Only 100 of 257 total aligned reads displayed.

GACTTCAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGAC  >  NC_000913/244960‑245139

gACTTCAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCa                                                                                         <  1:1084662/93‑1 (MQ=255)

 aCTTCAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAg                                                                                        >  1:6100716/1‑93 (MQ=255)

 aCTTCAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAg                                                                                        >  1:5336126/1‑93 (MQ=255)

  cTTGAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAgg                                                                                       <  1:1214914/93‑1 (MQ=255)

  cTTCAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAgg                                                                                       >  2:798616/1‑93 (MQ=255)

  cTTCAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAgg                                                                                       <  1:4847578/93‑1 (MQ=255)

       gCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAAt                                                                                  <  2:4052936/93‑1 (MQ=255)

       gCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAAt                                                                                  <  1:3003127/93‑1 (MQ=255)

       gCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAAt                                                                                  <  1:1491048/93‑1 (MQ=255)

        cTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATg                                                                                 <  2:3241674/93‑1 (MQ=255)

        cTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGAGCATg                                                                                 >  1:3919418/1‑93 (MQ=255)

         tCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATgg                                                                                <  2:3725508/93‑1 (MQ=255)

         tCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATgg                                                                                <  1:4686075/93‑1 (MQ=255)

          cGCAGANCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCNGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATggg                                                                               >  1:1809985/1‑92 (MQ=255)

            cAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGgcgc                                                                             <  1:2208795/93‑1 (MQ=255)

               aCCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCttt                                                                          >  2:1844535/1‑92 (MQ=255)

                ccACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTAt                                                                         <  1:2783238/93‑1 (MQ=255)

                 cacaCCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTAtt                                                                        <  1:5457261/93‑1 (MQ=255)

                   cacCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTAtttt                                                                      >  1:4618584/1‑93 (MQ=255)

                   cacCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTAtttt                                                                      >  2:5991612/1‑93 (MQ=255)

                    acCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGGATGGCGCTTATTTTg                                                                     >  2:5220133/1‑93 (MQ=255)

                     ccGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGc                                                                    <  1:2446099/93‑1 (MQ=255)

                      cGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTggc                                                                   >  2:2283066/1‑91 (MQ=255)

                      cGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGcc                                                                   <  2:5176672/93‑1 (MQ=255)

                       gAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCt                                                                  <  2:3018088/93‑1 (MQ=255)

                       gAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCt                                                                  >  2:4275230/1‑93 (MQ=255)

                          tgatgTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCACTTATTTTGCCTCgg                                                               <  2:4928220/93‑1 (MQ=255)

                           gatgTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCggg                                                              <  2:6302299/93‑1 (MQ=255)

                           gaggtgGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCggg                                                              <  2:5484399/90‑1 (MQ=255)

                             tgtgGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGa                                                            >  2:2698130/1‑93 (MQ=255)

                                ggTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCg                                                         >  1:931205/1‑93 (MQ=255)

                                ggTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCg                                                         >  2:1798025/1‑93 (MQ=255)

                                  tCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCgtg                                                       >  1:6283277/1‑93 (MQ=255)

                                  tCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCgag                                                       >  1:5672790/1‑91 (MQ=255)

                                   cACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCgtgt                                                      <  2:2295504/93‑1 (MQ=255)

                                   cACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCgtgt                                                      <  2:5807992/93‑1 (MQ=255)

                                      ggTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGt                                                   <  2:4606148/93‑1 (MQ=255)

                                       gTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGtt                                                  >  1:2223134/1‑93 (MQ=255)

                                       gTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATATCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGtt                                                  <  1:3381597/93‑1 (MQ=255)

                                        tGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGttt                                                 <  1:4742209/93‑1 (MQ=255)

                                        tGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGttt                                                 <  2:5107035/93‑1 (MQ=255)

                                         gATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTg                                                >  2:3833394/1‑93 (MQ=255)

                                          aTTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGa                                               >  1:2628354/1‑93 (MQ=255)

                                           ttGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGAt                                              <  2:5735658/93‑1 (MQ=255)

                                            tGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATg                                             >  2:5580313/1‑93 (MQ=255)

                                            tGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATg                                             >  1:1231162/1‑93 (MQ=255)

                                             gCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGc                                            <  2:1445423/93‑1 (MQ=255)

                                             gCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGc                                            >  1:3085595/1‑93 (MQ=255)

                                               aaCACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTTCCTCGGGGAGCGTGTAGTTTGATGCCa                                          >  1:1891859/1‑93 (MQ=255)

                                               aaCACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCa                                          <  1:3607465/93‑1 (MQ=255)

                                               aaCACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCa                                          <  2:1598276/93‑1 (MQ=255)

                                                acacAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAg                                         >  2:1931934/1‑93 (MQ=255)

                                                 cacaGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGt                                        >  2:4419769/1‑93 (MQ=255)

                                                  acaGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGtt                                       >  1:2906460/1‑93 (MQ=255)

                                                   caGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTtg                                      >  1:5624423/1‑93 (MQ=255)

                                                    aGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCCGTtgt                                     >  2:5341140/1‑93 (MQ=255)

                                                       cGCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGc                                  <  1:3751975/93‑1 (MQ=255)

                                                        gCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATTGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTTGTGGCAGTTGTGGCt                                 >  2:2466589/1‑93 (MQ=255)

                                                        gCTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCt                                 >  1:3300057/1‑93 (MQ=255)

                                                         cTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTg                                >  1:1734932/1‑93 (MQ=255)

                                                         cTGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTg                                >  2:1378844/1‑93 (MQ=255)

                                                          tGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGc                               <  1:872540/93‑1 (MQ=255)

                                                          tGACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGc                               >  1:4057534/1‑93 (MQ=255)

                                                           gACGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGca                              >  2:6128843/1‑93 (MQ=255)

                                                             cGGGCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGcaca                            <  2:3987888/93‑1 (MQ=255)

                                                               ggCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAAc                          <  2:3159399/93‑1 (MQ=255)

                                                               ggCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAAc                          <  2:4989718/93‑1 (MQ=255)

                                                                gCAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACt                         >  2:2196880/1‑93 (MQ=255)

                                                                 cAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACtt                        <  2:3542094/93‑1 (MQ=255)

                                                                 cAATGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACtt                        <  2:1221036/93‑1 (MQ=255)

                                                                  aaTGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTc                       <  2:2771822/93‑1 (MQ=255)

                                                                  aaTGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTc                       >  2:4451002/1‑93 (MQ=255)

                                                                  aaTGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTc                       <  2:2420889/93‑1 (MQ=255)

                                                                   aTGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCAAAACTTCg                      >  1:4160608/1‑93 (MQ=255)

                                                                    tGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACCACTTCGt                     >  2:5364301/1‑93 (MQ=255)

                                                                    tGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGt                     >  1:5166344/1‑93 (MQ=255)

                                                                    tGAAACCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGCGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGt                     <  1:3393237/93‑1 (MQ=255)

                                                                        aCCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCt                 >  2:4716817/1‑93 (MQ=255)

                                                                        aCCTGGCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCt                 <  1:4473613/93‑1 (MQ=255)

                                                                         ccTGGCAAAAGATTATATCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCtt                <  1:2021111/93‑1 (MQ=255)

                                                                            ggCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAAc             >  1:4922871/1‑93 (MQ=255)

                                                                            ggCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAAc             <  1:5394518/93‑1 (MQ=255)

                                                                            ggCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAAc             <  1:5044093/93‑1 (MQ=255)

                                                                            ggCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTAGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGAACAACTTCGTGGCTTAAc             <  1:2706826/93‑1 (MQ=255)

                                                                             gCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGGCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACg            >  1:1547560/1‑93 (MQ=255)

                                                                             gCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACg            <  1:678018/93‑1 (MQ=255)

                                                                             gCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACg            <  1:6010443/93‑1 (MQ=255)

                                                                             gCAAAAGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACg            <  2:3815208/93‑1 (MQ=255)

                                                                               aaaaGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGc          >  2:2957891/1‑93 (MQ=255)

                                                                                aaaGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCt         <  1:3264253/93‑1 (MQ=255)

                                                                                aaaGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCt         >  2:713144/1‑93 (MQ=255)

                                                                                 aaGATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCtt        >  1:5907/1‑93 (MQ=255)

                                                                                   gATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGGACAACTTCGTGGCTTAACGGCtttt      >  2:2020701/1‑91 (MQ=255)

                                                                                   gATTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCtttt      >  1:2321147/1‑91 (MQ=255)

                                                                                    aTTATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCt     >  2:1097619/1‑93 (MQ=255)

                                                                                     ttATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTTCTg    >  1:3152300/1‑93 (MQ=255)

                                                                                     ttATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTTCTg    >  2:2895372/1‑93 (MQ=255)

                                                                                      tATGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGa   <  2:3667392/93‑1 (MQ=255)

                                                                                       aTGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGAc  <  2:6111807/93‑1 (MQ=255)

                                                                                       aTGTCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGAc  >  1:1030827/1‑93 (MQ=255)

GACTTCAGCTCGCAGACCACACCGAATGATGTGGTCACGGTGATTGCAACACAGCCGCTGACGGGCAATGAAACCTGGCAAAAGATTATGCCAGGCGAATGGCGCTTATTTTGCCTCGGGGAGCGTGTAGTTTGATGCCAGTTGTGGCTGCACAACTTCGTGGCTTAACGGCTTGCTGAC  >  NC_000913/244960‑245139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`