BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	247,207	A→G	T166A (ACG→GCG)	yafL →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	247,207	0	A	G	99.6%	752.4 / ‑5.0	237	T166A (ACG→GCG)	yafL	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (107/129); total (107/130)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

Only 100 of 256 total aligned reads displayed.

TGGGGCGGTACGCGGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCACGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCATATCCAC  >  NC_000913/247117‑247296

tGGGGCGGTACGCGGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCcgcgcgcg                                                                                         >  1:3341881/1‑93 (MQ=255)

tGGGGCGGTACGCGGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCcgcgcgcg                                                                                         <  1:2946350/93‑1 (MQ=255)

  gggCGGTACGCGGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGc                                                                                       >  1:5896649/1‑93 (MQ=255)

      ggTACGCGGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAAt                                                                                   <  1:724010/93‑1 (MQ=255)

       gTACGCGGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTGTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCACTg                                                                                  >  1:4439986/1‑93 (MQ=255)

        tACGCGCCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGAGCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATga                                                                                 <  1:1760249/93‑1 (MQ=255)

          cgcgGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATgaga                                                                               <  2:2392798/93‑1 (MQ=255)

           gcgGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGAt                                                                              <  1:6253279/93‑1 (MQ=255)

           gcgGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGAt                                                                              >  1:2032239/1‑93 (MQ=255)

           gcgGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGAt                                                                              <  1:2890221/93‑1 (MQ=255)

              gCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTa                                                                           <  2:2189047/93‑1 (MQ=255)

                  gATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCAc                                                                       >  1:1038149/1‑93 (MQ=255)

                  gATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCAc                                                                       >  2:4540736/1‑93 (MQ=255)

                   aTAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACt                                                                      >  2:3549636/1‑93 (MQ=255)

                    tAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTa                                                                     <  2:3997004/93‑1 (MQ=255)

                     aaaGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTAt                                                                    <  1:2789238/93‑1 (MQ=255)

                      aaGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATc                                                                   <  1:5252575/93‑1 (MQ=255)

                      aaGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATc                                                                   <  1:3977220/93‑1 (MQ=255)

                       aGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCg                                                                  <  2:5006927/93‑1 (MQ=255)

                        ggCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGc                                                                 <  1:4241337/93‑1 (MQ=255)

                          cTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCg                                                               <  2:5239564/93‑1 (MQ=255)

                          cTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCg                                                               >  1:4853235/1‑93 (MQ=255)

                           tttGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCgg                                                              >  2:5086786/1‑93 (MQ=255)

                           tttGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCgg                                                              <  2:1304263/93‑1 (MQ=255)

                           tttGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCgg                                                              >  2:706651/1‑93 (MQ=255)

                           tttGACTGTAGCGGGGTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGGCAATGAGATGTACCACTATCGCCgg                                                              >  2:1176720/1‑93 (MQ=255)

                            ttGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCggg                                                             >  1:4908750/1‑93 (MQ=255)

                             tGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGc                                                            <  1:621901/93‑1 (MQ=255)

                               aCTGTAGCGGGTTGTTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCaa                                                          <  2:1113575/93‑1 (MQ=255)

                               aCTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCaa                                                          >  2:2245329/1‑93 (MQ=255)

                               aCTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCaa                                                          >  2:5307832/1‑93 (MQ=255)

                               aCTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCaa                                                          <  2:4349492/93‑1 (MQ=255)

                               aCTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCaa                                                          >  1:1948906/1‑93 (MQ=255)

                                cTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAAc                                                         <  2:2332673/93‑1 (MQ=255)

                                 tGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACg                                                        >  2:4177735/1‑93 (MQ=255)

                                  gTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGa                                                       >  1:6275382/1‑93 (MQ=255)

                                   tAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGAt                                                      <  1:817003/93‑1 (MQ=255)

                                     gCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATtg                                                    <  1:3692000/93‑1 (MQ=255)

                                     gCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATtg                                                    >  2:719148/1‑93 (MQ=255)

                                     gCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATtg                                                    >  2:3347451/1‑93 (MQ=255)

                                     gCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATtg                                                    >  1:2405609/1‑93 (MQ=255)

                                       gggTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATtgtg                                                  <  1:5710690/93‑1 (MQ=255)

                                       gggTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATtgtg                                                  <  2:2206549/93‑1 (MQ=255)

                                        ggttggttTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTTCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTgg                                                 >  2:2993777/1‑93 (MQ=255)

                                         gttggttTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGc                                                >  2:898052/1‑93 (MQ=255)

                                             gttTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGaac                                            >  2:1423465/1‑93 (MQ=255)

                                             gttTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGaac                                            >  2:1484649/1‑93 (MQ=255)

                                              ttttttATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGaaca                                           >  2:5942577/1‑93 (MQ=255)

                                              ttttttATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGaaca                                           >  2:5662245/1‑93 (MQ=255)

                                              ttttttATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGaaca                                           >  1:1183959/1‑93 (MQ=255)

                                               tttttATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGaacaa                                          <  1:4736480/93‑1 (MQ=255)

                                               tttttATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGaacaa                                          >  2:2234781/1‑93 (MQ=255)

                                                ttttATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGaacaac                                         <  2:4848756/93‑1 (MQ=255)

                                                ttttATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGaacaac                                         <  2:3086126/93‑1 (MQ=255)

                                                ttttATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGaacaac                                         >  2:2859471/1‑93 (MQ=255)

                                                ttttATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGaacaac                                         <  1:2459258/93‑1 (MQ=255)

                                                   tATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGAc                                      >  2:6208893/1‑93 (MQ=255)

                                                    aTGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGAcc                                     <  2:1074502/93‑1 (MQ=255)

                                                    aTGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGAcc                                     <  1:5413592/93‑1 (MQ=255)

                                                    aTGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGAcc                                     <  2:5217222/93‑1 (MQ=255)

                                                     tGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCt                                    >  2:2091478/1‑93 (MQ=255)

                                                     tGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCt                                    >  2:4068772/1‑93 (MQ=255)

                                                      gCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTg                                   <  1:5980622/93‑1 (MQ=255)

                                                         tACAACAAGATCCTTGAGGCTAAGCTGCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTgcgc                                >  2:1790541/1‑93 (MQ=255)

                                                          acaacaAGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGcc                               <  1:131466/93‑1 (MQ=255)

                                                           caacaaGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCg                              >  2:3851447/1‑93 (MQ=255)

                                                           caacaaGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCg                              <  2:1452171/93‑1 (MQ=255)

                                                            aacaaGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCgg                             <  1:3159757/93‑1 (MQ=255)

                                                              caaGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCgggg                           >  1:1923936/1‑93 (MQ=255)

                                                              caaGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCgggg                           >  2:6280052/1‑93 (MQ=255)

                                                               aaGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGga                          <  1:14697/93‑1 (MQ=255)

                                                                aGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGgag                         >  1:252162/1‑93 (MQ=255)

                                                                aGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGgag                         >  1:348460/1‑93 (MQ=255)

                                                                aGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGgag                         >  2:1365942/1‑93 (MQ=255)

                                                                aGATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCCGGgag                         >  2:1064170/1‑93 (MQ=255)

                                                                 gATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGgaga                        <  2:3890815/93‑1 (MQ=255)

                                                                 gATCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGgaga                        <  2:5337578/93‑1 (MQ=255)

                                                                  aTCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGAt                       <  2:481483/93‑1 (MQ=255)

                                                                  aTCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGAt                       <  1:4624759/93‑1 (MQ=255)

                                                                   tCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGAtt                      <  2:4461849/93‑1 (MQ=255)

                                                                   tCCTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGAtt                      >  2:3690415/1‑93 (MQ=255)

                                                                   tCCTTGAGCCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGAtt                      <  2:5461669/93‑1 (MQ=255)

                                                                    ccTTGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGAttt                     <  1:4068772/93‑1 (MQ=255)

                                                                      ttGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGc                   <  2:5604599/93‑1 (MQ=255)

                                                                      ttGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGc                   <  2:2793471/93‑1 (MQ=255)

                                                                      ttGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGc                   >  2:1977413/1‑93 (MQ=255)

                                                                       tGAGGCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCt                  <  1:3626582/93‑1 (MQ=255)

                                                                           gCTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGttt              <  1:4414923/93‑1 (MQ=255)

                                                                            cTAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGtttt             >  1:3722382/1‑93 (MQ=255)

                                                                             tAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGttttt            >  2:3393809/1‑93 (MQ=255)

                                                                             tAAGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGttttt            >  2:4445403/1‑93 (MQ=255)

                                                                              aaGCTCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTc           >  2:4043276/1‑93 (MQ=255)

                                                                                  tCCCGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCata       <  2:3161057/93‑1 (MQ=255)

                                                                                   cccGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCatat      >  2:590144/1‑93 (MQ=255)

                                                                                   cccGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCatat      <  2:2617357/93‑1 (MQ=255)

                                                                                   cccGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCatat      <  1:1384979/93‑1 (MQ=255)

                                                                                    ccGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCATATc     <  1:5059729/93‑1 (MQ=255)

                                                                                    ccGCGCGCGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCATATc     >  1:307813/1‑93 (MQ=255)

                                                                                       cgcgcgGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCATATCcac  <  2:2652346/93‑1 (MQ=255)

                                                                                       cgcgcgGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCATATCcac  <  1:908415/93‑1 (MQ=255)

TGGGGCGGTACGCGGCCTGATAAAGGCTTTGACTGTAGCGGGTTGGTTTTTTATGCCTACAACAAGATCCTTGAGGCTAAGCTCCCGCGCACGGCCAATGAGATGTACCACTATCGCCGGGCAACGATTGTGGCGAACAACGACCTGCGCCGGGGAGATTTGCTGTTTTTCCATATCCAC  >  NC_000913/247117‑247296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`