BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,207,023	A→G	D346G (GAC→GGC)	yehP →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,207,023	0	A	G	99.5%	638.3 / NA	198	D346G (GAC→GGC)	yehP	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (100/97); minor base T (0/1); total (100/98)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

TACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATC  >  NC_000913/2206933‑2207112

tACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGcc                                                                                         >  1:1663575/1‑93 (MQ=255)

 aCTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCcg                                                                                        >  2:4090352/1‑93 (MQ=255)

 aCTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCcg                                                                                        >  2:5384325/1‑93 (MQ=255)

     aCGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGAt                                                                                    >  1:4381480/1‑93 (MQ=255)

      cGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATa                                                                                   <  1:1418101/93‑1 (MQ=255)

       gCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATAc                                                                                  >  1:4554640/1‑93 (MQ=255)

          tCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGc                                                                               >  2:3890320/1‑93 (MQ=255)

            aGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGccc                                                                             >  1:1531433/1‑93 (MQ=255)

            aGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGccc                                                                             <  1:3581848/93‑1 (MQ=255)

            aGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGccc                                                                             <  1:2983813/93‑1 (MQ=255)

              gTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAg                                                                           <  2:5266569/93‑1 (MQ=255)

                 aaaaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGgcg                                                                        >  2:131876/1‑93 (MQ=255)

                 aaaaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGgcg                                                                        <  2:891964/93‑1 (MQ=255)

                  aaaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGgcgc                                                                       <  1:6112306/93‑1 (MQ=255)

                  aaaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGgcgc                                                                       >  1:4050881/1‑93 (MQ=255)

                  aaaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGgcgc                                                                       >  1:5397670/1‑93 (MQ=255)

                   aaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCt                                                                      >  2:2923410/1‑93 (MQ=255)

                   aaaGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCt                                                                      >  2:954667/1‑93 (MQ=255)

                     aGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTgg                                                                    <  1:5888521/93‑1 (MQ=255)

                          gtCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAAt                                                               <  2:3542997/93‑1 (MQ=255)

                           tCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATg                                                              <  1:1844067/93‑1 (MQ=255)

                            ccAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGt                                                             >  2:5886122/1‑93 (MQ=255)

                            ccAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGt                                                             <  2:1821833/93‑1 (MQ=255)

                             cAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTc                                                            >  2:1644611/1‑93 (MQ=255)

                              agagCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCg                                                           >  1:3735680/1‑93 (MQ=255)

                              agagCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCg                                                           <  2:619741/93‑1 (MQ=255)

                                agCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGgc                                                         <  1:1005323/93‑1 (MQ=255)

                                 gCGGCATCAAAGTGCTGGGACTGGCAGCTCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGgcg                                                        <  1:2533887/93‑1 (MQ=255)

                                 gCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGgcg                                                        >  1:5785744/1‑93 (MQ=255)

                                  cGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACATTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGgcgc                                                       <  1:2402162/93‑1 (MQ=255)

                                   ggCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGGGca                                                      >  1:1195994/1‑93 (MQ=255)

                                    gCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGcac                                                     >  1:5527946/1‑93 (MQ=255)

                                     cATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGcaca                                                    >  1:208472/1‑93 (MQ=255)

                                       tCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACaaa                                                  <  1:3220406/93‑1 (MQ=255)

                                       tCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACaaa                                                  >  1:3965692/1‑93 (MQ=255)

                                       tCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACaaa                                                  <  2:5296630/93‑1 (MQ=255)

                                        cAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAAt                                                 <  2:1556281/93‑1 (MQ=255)

                                         aaaGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATa                                                >  2:2179388/1‑93 (MQ=255)

                                          aaGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAg                                               >  1:1044709/1‑93 (MQ=255)

                                           aGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAgc                                              <  1:4166496/93‑1 (MQ=255)

                                           aGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAgc                                              <  2:490111/93‑1 (MQ=255)

                                            gTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAgcc                                             <  2:1039596/93‑1 (MQ=255)

                                            gTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAgcc                                             <  2:424515/93‑1 (MQ=255)

                                               cTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAgccgcc                                          <  2:2038321/93‑1 (MQ=255)

                                               cTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAgccgcc                                          <  1:3144853/93‑1 (MQ=255)

                                               cTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAgccgcc                                          <  2:1476979/93‑1 (MQ=255)

                                                tGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCa                                         >  1:2909956/1‑93 (MQ=255)

                                                tGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCa                                         <  1:6166342/93‑1 (MQ=255)

                                                 gggACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGTCGCCAt                                        >  2:5111391/1‑93 (MQ=255)

                                                 gggACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCAt                                        >  2:315463/1‑93 (MQ=255)

                                                  ggACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATg                                       >  2:2541281/1‑93 (MQ=255)

                                                   gACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGa                                      <  2:6052814/93‑1 (MQ=255)

                                                      tGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGc                                   <  2:3820756/93‑1 (MQ=255)

                                                      tGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGc                                   >  2:3675974/1‑93 (MQ=255)

                                                      tGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGc                                   <  2:1911945/93‑1 (MQ=255)

                                                       ggCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGcc                                  <  1:3396304/93‑1 (MQ=255)

                                                       ggCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGcc                                  >  2:330251/1‑93 (MQ=255)

                                                        gCAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCg                                 <  2:664754/93‑1 (MQ=255)

                                                         cAGCGCTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCgg                                <  2:5941955/93‑1 (MQ=255)

                                                            cgcTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCg                             <  2:688083/93‑1 (MQ=255)

                                                             gcTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGa                            >  2:5354626/1‑93 (MQ=255)

                                                             gcTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGa                            >  2:6129468/1‑93 (MQ=255)

                                                              cTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAg                           >  1:941287/1‑93 (MQ=255)

                                                              cTCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAg                           >  2:4292195/1‑93 (MQ=255)

                                                               tCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGc                          >  2:4762340/1‑93 (MQ=255)

                                                               tCGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGc                          >  1:5334961/1‑93 (MQ=255)

                                                                cGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCt                         <  1:196414/93‑1 (MQ=255)

                                                                cGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCt                         <  1:793875/93‑1 (MQ=255)

                                                                cGATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCt                         <  2:4730874/93‑1 (MQ=255)

                                                                 gATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTg                        >  2:521132/1‑93 (MQ=255)

                                                                 gATAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTg                        >  2:4302397/1‑93 (MQ=255)

                                                                  aTAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTgg                       >  1:2260640/1‑93 (MQ=255)

                                                                  aTAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTgg                       >  2:5567428/1‑93 (MQ=255)

                                                                   tAGCACCGCAACACGTTGCTATGGCCGCGATCCGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGc                      <  1:4705800/93‑1 (MQ=255)

                                                                   tAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGc                      <  1:983737/93‑1 (MQ=255)

                                                                   tAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGc                      >  2:861198/1‑93 (MQ=255)

                                                                   tAGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGc                      <  1:6153167/93‑1 (MQ=255)

                                                                    aGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGca                     <  2:3454930/93‑1 (MQ=255)

                                                                    aGCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGca                     >  1:327265/1‑93 (MQ=255)

                                                                     gCACCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcat                    >  2:4026595/1‑93 (MQ=255)

                                                                       aCCGCAACCCGTTGCTATGGCCGCTATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcatca                  <  2:1396173/93‑1 (MQ=255)

                                                                       aCCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcatca                  >  1:3681867/1‑93 (MQ=255)

                                                                       aCCGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcatca                  <  2:2786123/93‑1 (MQ=255)

                                                                        ccGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGcatcat                 <  1:3090396/93‑1 (MQ=255)

                                                                         cGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATg                >  1:5917185/1‑93 (MQ=255)

                                                                         cGCAACACCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATg                <  2:5333496/93‑1 (MQ=255)

                                                                             acacCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCtt            >  1:4679417/1‑93 (MQ=255)

                                                                              cacCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCttt           >  2:5677534/1‑92 (MQ=255)

                                                                               acCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCtttt          >  2:55329/1‑91 (MQ=255)

                                                                               acCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCtttg          >  2:3629155/1‑91 (MQ=255)

                                                                               acCTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGc          <  1:5779432/93‑1 (MQ=255)

                                                                                ccTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCttttg         >  2:1611698/1‑90 (MQ=255)

                                                                                 cTTGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCgg        <  1:5286436/93‑1 (MQ=255)

                                                                                  ttGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTTCggg       >  1:5013153/1‑92 (MQ=255)

                                                                                  ttGCTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGga       <  1:6260646/93‑1 (MQ=255)

                                                                                     cTATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGaa    <  1:5622169/93‑1 (MQ=255)

                                                                                      tATGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTTCGGGGaaa   >  1:1714440/1‑92 (MQ=255)

                                                                                      tATGGCCGCGATACAGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTTTGGAGGAt   >  2:3849985/1‑93 (MQ=255)

                                                                                       aTGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATc  <  2:5135245/93‑1 (MQ=255)

                                                                                       aTGGCCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATc  <  2:4554640/93‑1 (MQ=255)

TACTGACGCATCAGGTGAAAAAGTGTGTCCAGAGCGGCATCAAAGTGCTGGGACTGGCAGCGCTCGATAGCACCGCAACACCTTGCTATGACCGCGATACGGCCCAGGCGCTGGTTAATGTCGGCGCACAAATAGCCGCCATGACGCCGGGCGAGCTGGCATCATGGCTTGCGGAGAATC  >  NC_000913/2206933‑2207112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`