BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,232,157	T→C	S35S (AGT→AGC)	b2146 →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,232,157	0	T	C	99.5%	611.7 / NA	190	S35S (AGT→AGC)	b2146	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (91/98); minor base A (1/0); total (92/98)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

TTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCG  >  NC_000913/2232067‑2232245

ttATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCa                                                                                        <  2:560799/93‑1 (MQ=255)

  aTCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAgg                                                                                      >  2:1112536/1‑93 (MQ=255)

   tCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGc                                                                                     >  1:988546/1‑93 (MQ=255)

     tGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCTATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCtt                                                                                   <  1:2776165/93‑1 (MQ=255)

      ggATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGACAGGCTTg                                                                                  >  1:2570757/1‑93 (MQ=255)

       gATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGc                                                                                 <  1:4934197/93‑1 (MQ=255)

        aTGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGcc                                                                                <  1:854435/93‑1 (MQ=255)

         tGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGccc                                                                               >  1:5016251/1‑93 (MQ=255)

            actcactcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGc                                                                            >  2:4297324/1‑93 (MQ=255)

            actcactcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGc                                                                            <  1:812831/93‑1 (MQ=255)

            actcaatcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTAACGACGCTCCCTGTAGCCAGGCTTGTGCGGc                                                                            >  2:4409549/1‑93 (MQ=255)

              tcactcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGcac                                                                          <  2:3669764/93‑1 (MQ=255)

                actcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAg                                                                        >  2:4442408/1‑93 (MQ=255)

                 ctcGGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGa                                                                       <  1:4192105/93‑1 (MQ=255)

                 ctcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGa                                                                       >  2:1365468/1‑93 (MQ=255)

                 ctcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGa                                                                       >  1:1280141/1‑93 (MQ=255)

                 ctcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGa                                                                       >  2:655973/1‑93 (MQ=255)

                  tcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGAc                                                                      <  2:1732388/93‑1 (MQ=255)

                  tcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGAc                                                                      >  1:1249984/1‑93 (MQ=255)

                   ccGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGAcc                                                                     >  1:730298/1‑93 (MQ=255)

                    cGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCg                                                                    >  1:2565137/1‑93 (MQ=255)

                      gCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGAt                                                                  >  1:2098700/1‑93 (MQ=255)

                       cTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATTTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATc                                                                 <  1:1944672/93‑1 (MQ=255)

                       cTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATc                                                                 >  2:2289548/1‑93 (MQ=255)

                        ttttACGTCTTTACTGGCGATTAAATAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATcc                                                                <  2:4018982/93‑1 (MQ=255)

                        ttttACGTCTTTACTGGCGATTAAAGAATCCTCTCGCTTTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATcc                                                                <  1:3501408/93‑1 (MQ=255)

                        ttttACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATcc                                                                >  1:3953294/1‑93 (MQ=255)

                         tttACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCg                                                               <  2:3604/93‑1 (MQ=255)

                          ttACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCgg                                                              <  1:2431399/93‑1 (MQ=255)

                          ttACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCgg                                                              <  2:297054/93‑1 (MQ=255)

                           tACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCggg                                                             >  1:5654676/1‑93 (MQ=255)

                           tACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCggg                                                             <  2:2165384/93‑1 (MQ=255)

                           tACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCggg                                                             <  1:4126116/93‑1 (MQ=255)

                            aCGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCgggg                                                            <  2:1597817/93‑1 (MQ=255)

                             cGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGa                                                           >  1:4802113/1‑93 (MQ=255)

                              gTCTTTACTGGCGATTAAAGTAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGaa                                                          <  2:1343797/93‑1 (MQ=255)

                               tCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGCTCCGGGGaaa                                                         >  2:5123173/1‑93 (MQ=255)

                                 tttACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAAtt                                                       >  1:5976429/1‑93 (MQ=255)

                                   tACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTa                                                     >  1:4582360/1‑93 (MQ=255)

                                     cTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTAtt                                                   <  2:2387956/93‑1 (MQ=255)

                                     cTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTAtt                                                   <  1:2753965/93‑1 (MQ=255)

                                     cTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTAtt                                                   <  2:4777751/93‑1 (MQ=255)

                                      tGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTc                                                  <  1:395522/93‑1 (MQ=255)

                                      tGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTc                                                  >  2:2315790/1‑93 (MQ=255)

                                      tGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTc                                                  <  2:5167924/93‑1 (MQ=255)

                                      tGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTc                                                  >  1:4857100/1‑93 (MQ=255)

                                      tGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTc                                                  <  2:5373373/93‑1 (MQ=255)

                                        gCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGc                                                <  1:3843466/93‑1 (MQ=255)

                                          gATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTc                                              >  1:2549095/1‑93 (MQ=255)

                                            ttAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCaa                                            <  2:4203089/93‑1 (MQ=255)

                                            ttAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCaa                                            <  1:2078637/93‑1 (MQ=255)

                                             tAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAAt                                           <  2:4506840/93‑1 (MQ=255)

                                             tAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAAt                                           >  2:1753461/1‑93 (MQ=255)

                                              aaaGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATc                                          <  2:3736334/93‑1 (MQ=255)

                                               aagaagCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCt                                         >  2:1780905/1‑93 (MQ=255)

                                                agaagCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTa                                        >  1:2115168/1‑93 (MQ=255)

                                                agaagCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTa                                        >  2:2928285/1‑93 (MQ=255)

                                                   agCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACtt                                     <  1:284492/93‑1 (MQ=255)

                                                   agCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACtt                                     <  1:3622482/93‑1 (MQ=255)

                                                   agCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACtt                                     <  1:5059624/93‑1 (MQ=255)

                                                   agCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACtt                                     >  1:1097044/1‑93 (MQ=255)

                                                     ccTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTc                                   <  2:3264362/93‑1 (MQ=255)

                                                     ccTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTc                                   <  1:1437412/93‑1 (MQ=255)

                                                      ctctCGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCg                                  <  1:1745822/93‑1 (MQ=255)

                                                       tctcGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGt                                 >  1:974264/1‑93 (MQ=255)

                                                       tctcGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGt                                 >  2:1865512/1‑93 (MQ=255)

                                                       tctcGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGt                                 >  1:1850344/1‑93 (MQ=255)

                                                       tctcGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGt                                 >  1:4801896/1‑93 (MQ=255)

                                                          cGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAt                              <  2:3169554/93‑1 (MQ=255)

                                                          cGCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAt                              >  2:4111228/1‑93 (MQ=255)

                                                           gCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAtt                             >  2:4258964/1‑93 (MQ=255)

                                                           gCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAtt                             >  1:531092/1‑93 (MQ=255)

                                                           gCTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAtt                             >  2:5665169/1‑93 (MQ=255)

                                                            cTGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAttt                            <  1:4257977/93‑1 (MQ=255)

                                                             tGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAtttt                           >  2:2094176/1‑93 (MQ=255)

                                                             tGTTTATTATGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAtttt                           >  1:5474312/1‑93 (MQ=255)

                                                                ttattaGGTCACGACGCTCACTGTAGCTAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTaaa                        <  2:1480188/93‑1 (MQ=255)

                                                                  attatGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAgg                      <  1:238315/93‑1 (MQ=255)

                                                                  attatGTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAgg                      <  1:4697693/93‑1 (MQ=255)

                                                                       gTCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTg                 >  1:5262559/1‑93 (MQ=255)

                                                                        tCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGc                <  1:642300/93‑1 (MQ=255)

                                                                        tCACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGc                <  2:2606851/93‑1 (MQ=255)

                                                                         cACGACGCTCCCTGTAGCCAGGCTTGCCCGGCACCGACCGATCCGGGGAAATTTATTCGCTCCATCTACTTTCGTTATTTTCACGGCGCTGcc               >  1:5920906/1‑93 (MQ=255)

                                                                           cgacgCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCga             <  2:3532944/93‑1 (MQ=255)

                                                                            gacgCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCgag            <  2:5557503/93‑1 (MQ=255)

                                                                            gacgCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCgag            >  2:6094525/1‑93 (MQ=255)

                                                                             acgCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCgaga           >  2:1199888/1‑93 (MQ=255)

                                                                              cgCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGAc          <  2:5857626/93‑1 (MQ=255)

                                                                              cgCTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGAc          <  1:4457527/93‑1 (MQ=255)

                                                                                cTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACaa        >  1:157959/1‑93 (MQ=255)

                                                                                cTCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACaa        <  2:2734950/93‑1 (MQ=255)

                                                                                 tCCCTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAAt       >  2:5884254/1‑93 (MQ=255)

                                                                                  cccTGTAGCCAGGCTTGCCCGGCTCAGGCCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAAtt      <  2:1962055/93‑1 (MQ=255)

                                                                                  cccTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAAtt      <  1:3897056/93‑1 (MQ=255)

                                                                                  cccTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAAtt      <  1:5363703/93‑1 (MQ=255)

                                                                                   ccTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTc     <  1:1032272/93‑1 (MQ=255)

                                                                                   ccTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTc     >  2:808334/1‑93 (MQ=255)

                                                                                    cTGTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTcg    >  2:3803644/1‑93 (MQ=255)

                                                                                      gTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTcgcg  >  2:3289409/1‑93 (MQ=255)

                                                                                      gTAGCCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTcgcg  >  1:1109817/1‑93 (MQ=255)

TTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCG  >  NC_000913/2232067‑2232245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`